BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,730,870	T→C	noncoding (288/1542 nt)	rrsG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,730,870	0	T	C	97.4%	87.8 / ‑7.0	38	noncoding (288/1542 nt)	rrsG	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/26); minor base G (0/1); total (11/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCA  >  NC_000913/2730703‑2731102

cccTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                                                                                                                                                         <  1:102651/249‑1 (MQ=11)

    tcctccCCGCTGAAAGTGCGTTACACCACGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTccc                                                                                                                                                    <  1:482917/250‑1 (MQ=9)

    tcctccCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTccc                                                                                                                                                    <  1:42120/250‑1 (MQ=14)

       tacccGCTGAAAGAACTTTACAACCCGAAGGCCTTCTTCATCCACGCGGCATGCCTGCATCAGGCTTGCGCCCATTGCGCACTATTCCCCTCCGCTGCCTCCCGTAGGAGGCTGGACCGTGTCTCAGTTCCAGCG‑TGG‑CTGGTCCTCCTCTCAGACCCGCCAGGGATCGTCCCCTCGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGTTGGCAAGTGGCCCGAAGGTACCCctc                                                                                                                                                >  2:23014/3‑251 (MQ=11)

        ccccGCTGAAAGTACTTTACATCCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCct                                                                                                                                                 <  1:169298/249‑1 (MQ=14)

        ccccGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct                                                                                                                                               <  1:184214/251‑1 (MQ=14)

               gAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt                                                                                                                                        <  2:356150/251‑1 (MQ=14)

                  aGTACTTTACAACCCGAAGGCCTTCGTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc                                                                                                                                     <  1:37566/251‑1 (MQ=14)

                         tACAACCCGAAGGACTTCTTCATACTCGCGGCATGGCGGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TTG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGATGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAtt                                                                                                                               <  2:374035/250‑1 (MQ=255)

                            accccGAAAGCCTTCTACATTCACGCGTCTGGGCTGCTTCGGTCTTGCGCCCATTGTGCAATAGCCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCCGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGCCCAGCCAGGTATCGTCGCCTTGGTGGGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATTGCCGTAGGCCCGAAGGGCCCCCTCTTTGGTCTTGCTACATTATGc                                                                                                                           <  2:269748/249‑1 (MQ=11)

                                    ggCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGCGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTAtt                                                                                                                     >  1:266587/1‑249 (MQ=18)

                                     gCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTaac                                                                                                                  <  1:99137/251‑3 (MQ=18)

                                         tcttcATACACGCGGCATGGCTGCATCAGGCTTTCTCCCATTGTGCAATATGCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGAGCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCAGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAc                                                                                                               <  2:371273/250‑1 (MQ=255)

                                         tcttcATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAcc                                                                                                              >  2:112454/1‑251 (MQ=18)

                                             cATACACGCGGCATGGCCGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGTTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGtt                                                                                                           <  2:329811/250‑1 (MQ=18)

                                                acacGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAGCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTcc                                                                                                        <  1:468982/250‑1 (MQ=18)

                                                 cacGCGGCATGGATGCTCCAGGCTTGTGCCCATTGTGCAATATCCCCCTCTGCTGCCTCCCGTAGGAGTCTGGACCGTGGCTCATTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGCCCTGCCAGGGATCGCCGCCTTGGTGAGCCGTTACCCCACCACCAAGCTAATCCCATCTGGGCACCTCCTATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTCCCGTTTCCag                                                                                                      <  2:74643/251‑1 (MQ=11)

                                                     cgGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCagtagt                                                                                                  <  1:98600/251‑1 (MQ=18)

                                                                caacAGGCTGGCGCCCATTGTGCTATAGTCCCCACTGCTGCCTCCCGTAGGTGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCATGGATCGTCGCCTTGGTGAGCCGTTACCCCGCCAACAAGCTAACCCCATCTGGGCACATCCTATGGCATGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCttc                                                                                       <  2:85912/248‑3 (MQ=11)

                                                                    aGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATc                                                                                    >  1:28296/1‑250 (MQ=18)

                                                                            gcCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTGCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCCCCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCGCCATCAGGCAGCtt                                                                           <  2:194785/251‑1 (MQ=255)

                                                                             cccATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTc                                                                          >  2:11983/1‑251 (MQ=21)

                                                                                   gtgCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGAc                                                                    <  1:253945/251‑1 (MQ=21)

                                                                                        atatTCCCCACTGCTGCCGCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTAc                                                               <  1:402544/251‑1 (MQ=21)

                                                                                               ccACTGCTGCCTCCCGTAGGAGTCTTGACCGNGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCCCCCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCg                                                        <  2:220033/251‑1 (MQ=255)

                                                                                                   tgctgcCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTccc                                                    >  1:145434/1‑250 (MQ=21)

                                                                                                    gctgcCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTcc                                                     <  1:173262/249‑1 (MQ=25)

                                                                                                           cccGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTc                                              <  1:357814/249‑1 (MQ=25)

                                                                                                             cGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCa                                          <  1:99459/251‑1 (MQ=21)

                                                                                                              gTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTc                                           >  1:47972/1‑249 (MQ=25)

                                                                                                                         gACCGGGTCGCAGTTCC‑GTGGTGG‑CTGTTCCTCCTCCCAGCCCAGCCAGGGATCGCCGCCTTGGTGAGCCGTTCCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGCCATTATGCGGTATTCGCTACCGTTTCCAGTAGTTACCCCCCCCCAGCAGGCAGCTTCCCAGACATTTCTCACCCGTCCGCCACTCGTCAGCGAAGCAGCaa                              <  2:47972/251‑1 (MQ=11)

                                                                                                                               gtCTCAGTTC‑AGGG‑TGGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTGGGTGAGCCGTTCCCCCCCCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTGTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctgc                         <  2:145434/250‑1 (MQ=11)

                                                                                                                                tctcAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCtt                       >  2:451387/1‑251 (MQ=38)

                                                                                                                                   cAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCt                    >  1:122384/1‑251 (MQ=38)

                                                                                                                                            ggttgG‑CTGGTCATCCTCGCAGACCAGCGAGGGCTCGTCGCCTTGGTTGGCCGTTACCCCCCCAACAAGCTAACCCCTTCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCGCTTTGGTCTGGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGGCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt            <  2:260661/248‑1 (MQ=14)

                                                                                                                                             g‑tgG‑CTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc          >  1:451070/1‑251 (MQ=38)

                                                                                                                                                    tGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCACCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCCGGCAGCTTCCCCGACATTACTCACCCGTCCGCCACTCGTCACCGAAACAGCAAGCTGCTTCCTGTTACCGTTCGACtt     >  2:324826/1‑251 (MQ=255)

                                                                                                                                                        cATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTCCCCCACCAACAAGCTAATCCCATCGGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa  <  1:428059/250‑1 (MQ=255)

CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG‑TGG‑CTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCA  >  NC_000913/2730703‑2731102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`