Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	186.8 / NA	53	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/23);  total (30/23)

GCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCC  >  NC_000913/2112931‑2113420                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gcttggacattacatactcctgtcaagtgtgntcaccgcgttgaatgctcgcggcctggaatgttcgcaattaagtatacaaagtacttattaaagtataaatatcttatccaggcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGCCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCActgcgtgct                                                                                                                                                                                                                                                  <  2:240880‑M2/115‑2 (MQ=255)     ggccattacatactcctgtaaggttggagcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTTCCTCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGcc                                                                                                                                                                                                                                              <  2:275430‑M2/119‑1 (MQ=255)           tacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTatga                                                                                                                                                                                                                                         <  1:348079‑M2/124‑1 (MQ=255)                      gtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttacccatgtttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCGCAGTTTGATTAATAGAGGTATTAATGCACGCGACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGc                                                                                                                                                                                                                               <  2:425569‑M2/134‑1 (MQ=255)                                 ccaccgcgctgaacgatcgtggcctggaatgtacgcaaataattatacaaagtacttattaaagtataaagagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGGATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTGACCCACgcgc                                                                                                                                                                                                                  <  2:295465‑M2/147‑1 (MQ=255)                                   cccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaattacttattaaagtttaaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCgcgc                                                                                                                                                                                                                <  1:229015‑M2/149‑1 (MQ=255)                                          tgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAAccc                                                                                                                                                                                                         >  1:333022‑M2/96‑251 (MQ=255)                                          tgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAAccc                                                                                                                                                                                                         >  2:273845‑M2/96‑251 (MQ=255)                                                     ggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCa                                                                                                                                                                                              >  2:72583‑M2/85‑250 (MQ=255)                                                         tggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGc                                                                                                                                                                                         >  1:352440‑M2/81‑251 (MQ=255)                                                          ggaatgttcgcaaataattatacaaagtacttattaaagtataaatagcttatccatgcttttatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGc                                                                                                                                                                                         <  1:400360‑M2/171‑1 (MQ=255)                                                           gaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCt                                                                                                                                                                                        >  2:174480‑M2/79‑250 (MQ=255)                                                                ttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTACTAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGt                                                                                                                                                                                  >  2:354146‑M2/74‑251 (MQ=255)                                                                ttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGTCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAACCCCG‑AAAACTTCAAACGCTAATTGt                                                                                                                                                                                  >  1:106823‑M2/74‑251 (MQ=255)                                                                  cgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTc                                                                                                                                                                                 <  1:384037‑M2/179‑1 (MQ=255)                                                                   gcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCtt                                                                                                                                                                               >  2:127345‑M2/71‑251 (MQ=255)                                                                       ataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTTGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                                            >  2:10188‑M2/67‑250 (MQ=255)                                                                       ataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                                            >  1:83510‑M2/67‑250 (MQ=255)                                                                       agaagtatacacagttcttattaacgtataaatatcttttccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAACTAAGCGTCTCTCAGTTTGCTGACTAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTTCAGCTACCCGAGCACTGCGTGCATGCCTATGATGTGACGAGCTTTACCCACGCGCGCTAAACCCG‑AAAAATTCAAACTCTAATTGTCTTACCa                                                                                                                                                                           <  2:171549‑M2/185‑1 (MQ=255)                                                                             atacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGc                                                                                                                                                                     >  2:482408‑M2/61‑251 (MQ=255)                                                                              tacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTCATTGTCTTACCAATCCgcc                                                                                                                                                                    >  1:450468‑M2/60‑250 (MQ=255)                                                                               acaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAAtt                                                                                                                                                                                                <  1:49997‑M2/164‑1 (MQ=255)                                                                               acaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAAtt                                                                                                                                                                                                >  2:49997‑M2/59‑222 (MQ=255)                                                                                      acttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAAc                                                                                                                                                            >  1:196927‑M2/52‑251 (MQ=255)                                                                                         tattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTATTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACa                                                                                                                                                           >  1:431153‑M2/49‑249 (MQ=255)                                                                                          attaaagtataaatagcttatccatgcttatatgcttacggatttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAACTTCAAACGCTNATTGTCTTACCAATCCGCTCTGGAAACAAgg                                                                                                                                                        >  2:222978‑M2/48‑251 (MQ=255)                                                                                                  ataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCt                                                                                                                                                >  2:393360‑M2/40‑251 (MQ=255)                                                                                                      atagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGaa                                                                                                                                            <  1:70554‑M2/216‑1 (MQ=255)                                                                                                          cttatccatgcttatatgcttacggctttatTTATTCCATATTAATTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACt                                                                                                                                        >  1:414828‑M2/32‑251 (MQ=255)                                                                                                            tatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACtt                                                                                                                                       <  1:49995‑M2/221‑1 (MQ=255)                                                                                                             atccatgcttatatgcttgcggctttaaTTATTCCATCTTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTAGATTAATAGAGATATTAATGCAAGCTACCACCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGGTGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCCAACGCTCATTGTCTTACCAATCCACTCTGGAAACAAGGAAACTCCTGGAAAAATTTg                                                                                                                                     >  2:410270‑M2/29‑251 (MQ=255)                                                                                                              tccatgcttatatgcttacggctttatTTATTCCTTATTCCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCATTTTGATTAATAGAGGTATTAATGCTCGCTACCGCCCCTGGATTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTg                                                                                                                                     <  1:56845‑M2/223‑1 (MQ=255)                                                                                                                 atgcttatatgcttacggctttatTTATTCCATATTACTTCAGAACATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCATGAAACAAGGAAAATCCTGGAAAACTTTGAAt                                                                                                                                  >  2:24684‑M2/25‑250 (MQ=255)                                                                                                                              tacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaa                                                                                                                                <  1:347381‑M2/228‑1 (MQ=255)                                                                                                                              tacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaa                                                                                                                                >  2:347381‑M2/12‑239 (MQ=255)                                                                                                                                   ctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGAc                                                                                                                                                                                                                                   >  1:346771‑M2/7‑136 (MQ=255)                                                                                                                                   ctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGAc                                                                                                                                                                                                                                   <  2:346771‑M2/130‑1 (MQ=255)                                                                                                                                            ttCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTAtt                                                                                                      >  1:283772/1‑251 (MQ=255)                                                                                                                                                atatTACTTCAGAGCAAACTGTGAAATAAGACGCTCTCAAATAGACTAATAGAAGAAAAACTGCACGAAAAAGACCCTGGCATTACACCTACCAGAGCACTACGTGCATGACACTGATGTGACGAGCAATACCCACTAAAACAACACCCGCAAAAAAACAA‑CGCTAATTGTCAAACCAATCCGCTCTGACAACAAGGAACACCCTGGAAAACTTTACATAAAACCCTACTGCTAACTCGTTGTTATTCTg                                                                                                   >  2:414479/1‑250 (MQ=255)                                                                                                                                                  atTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATg                                                                                                >  1:223992/1‑251 (MQ=255)                                                                                                                                                     aCTTCAGAGCATGCTGTGAAATAAGCGGCTATCAGTTTGATTAATAGAGGTATTAATGCACGCTACAGCCCCTGGCTTTACAGCTACCAGAGCACTGCGAGCATGCCTATGATGTTACGCGCGTTACCCACTCGCGCGAAACCCG‑AAAAATTCAAACGCTACTTGTCTTACCAAACCGCTCTGGAAACAAGGAAAAACCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTGGTTATTCTGATGGtt                                                                                             >  2:464039/1‑251 (MQ=255)                                                                                                                                                       tacatcgcatcaggagaaaataGCGCCTCTCATTGTGATTAATAGAGGTATCAATGCACTGTACCGACCCTGGCTTTACAGCTACCATAGCACTGCGTGCAGGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAACTCAAACGCTAATTGTCTTACCAATCCGCTCTGCAAACAAGTAAAATCCTGTAAAACTTGTACGAAAACCCTACTGCTAACTCGTTGTTATTTTGAGGGttt                                                                                            <  2:63393/229‑1 (MQ=255)                                                                                                                                                        tcagggcaagcgtgaaaaTAATCGGCTCTCAGTTTGATAAATAGAGGTATTACTGCACGCGACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATGCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTGTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATggatt                                                                                            <  2:354998/234‑4 (MQ=255)                                                                                                                                                                    gtgAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                                                <  1:310186/249‑1 (MQ=255)                                                                                                                                                                      gAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAAGGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACACCGGCa                                                                            <  1:73252/251‑1 (MQ=255)                                                                                                                                                                            aGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGCATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGaaa                                                                       >  2:482249/1‑249 (MQ=255)                                                                                                                                                                                     cAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGcaaca                                                             <  1:211174/251‑1 (MQ=255)                                                                                                                                                                                               aaTAGAGGTATTAATGCCCGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtttt                                                   <  2:196927/251‑1 (MQ=255)                                                                                                                                                                                                  agagGTATTATTGCATGATCCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGGGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCGAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgc                                                 <  2:254698/250‑1 (MQ=255)                                                                                                                                                                                                    agGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgctgc                                              >  1:307899/1‑251 (MQ=255)                                                                                                                                                                                                           aatgctcccTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAAttt                                        <  2:271893/242‑1 (MQ=255)                                                                                                                                                                                                                     aCCGCCCCTGGCTTTACAGCCACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAAGTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTTTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACGTTTTCTGCGAATGTTCACTTCCGtt                             <  1:143215/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCGACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTTTCAACCGtt        >  2:406033/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAACATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGGGAATTTTCAATGNCGTTATAATTTTCTTATCAACCGTTAAATcc  >  2:212044/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCG‑AAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCC  >  NC_000913/2112931‑2113420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑