Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,954	A→G	intergenic (+23/+78)	yoeF → / ← tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,954	0	A	G	100.0%	193.2 / NA	52	intergenic (+23/+78)	yoeF/tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

GCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATC  >  NC_000913/2078709‑2079197                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    actttctcgtgttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGgatgat                                                                                                                                                                                                                                                <  1:101486‑M2/30‑1 (MQ=255)   tttctcgtgttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGatgatg                                                                                                                                                                                                                                               >  1:6562‑M2/220‑250 (MQ=255)           gttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGAt                                                                                                                                                                                                                                       <  1:400706‑M2/39‑1 (MQ=255)            ttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgagttctgaaatccaggctattgattcaacgtcagcgacagctctacgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATac                                                                                                                                                                                                                                     >  1:440190‑M2/211‑251 (MQ=255)              aacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATacac                                                                                                                                                                                                                                   >  1:16060‑M2/209‑251 (MQ=255)                cgtccgacaaaatgcgtcattgacagcggtttatgtggcttgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaccttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATacac                                                                                                                                                                                                                                   >  2:110707‑M2/207‑249 (MQ=255)                 gtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATacac                                                                                                                                                                                                                                   >  1:40801‑M2/206‑248 (MQ=255)                          aatgcgtcattgacagcggtttctgtggattgacacatttcattccgataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTAGGATGAGGATGATGACGGTGATACACAGCTAAGAATc                                                                                                                                                                                                                        >  2:164652‑M2/197‑250 (MQ=255)                             gcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatg                                                                                                                                                                                                                    <  2:63375‑M2/58‑1 (MQ=255)                                               tctgtggattgacacatttaattcagagaatgaattaatgcgactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCg                                                                                                                                                                                                   <  2:340216‑M2/75‑1 (MQ=255)                                                  gttaagggccacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCa                                                                                                                                                                                               <  1:367805‑M2/79‑1 (MQ=255)                                                        tgacacatgtcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaagagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt                                                                                                                                                                                          <  2:476999‑M2/84‑1 (MQ=255)                                                            acatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTg                                                                                                                                                                                     >  1:309330‑M2/163‑251 (MQ=255)                                                                   attcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATaaa                                                                                                                                                                               >  1:486218‑M2/156‑250 (MQ=255)                                                                             tgaagtaatgctactgccggaacaaccagcacacgggcattgagttctgaaatccaggctattgattcaacgccagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggagtggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACAGAGATAAGAATCAATGAGCAATGGGGGTTGCCGGGCAACCCTCATTGAATAAAACGGAAATTAc                                                                                                                                                                    >  2:338202‑M2/146‑251 (MQ=255)                                                                                    atgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgctt                                                                                                                                                             <  2:472715‑M2/113‑1 (MQ=255)                                                                                    atacgactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggatattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattattcggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgctt                                                                                                                                                             <  2:16449‑M2/113‑1 (MQ=255)                                                                                     tgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgcttc                                                                                                                                                            >  2:57012‑M2/138‑251 (MQ=255)                                                                                      gcttctggcggaacaaacagcaaacgggcattgtgtgctgaaatccaggctagtgattcaccgtcagcgacagatctgcgctgtaaaagaaattgtaaaacaacgtaatagaattatacggaatggtggcgactatAATTTCGATTGGGTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCGCATTGAATACATCGGGAattacttcgcatct                                                                                                                                                           <  2:348724‑M2/115‑5 (MQ=255)                                                                                          ctgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGt                                                                                                                                                       >  2:47218‑M2/133‑251 (MQ=255)                                                                                              cggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGttttt                                                                                                                                                   >  1:389001‑M2/129‑251 (MQ=255)                                                                                                gaacaaccagcaaacgggcattgtgttctgaaatcaaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaaaggtggcgactatAATTTCGATTGGTTCTCGGCTGAGGATGATGACGGCGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAACCGGGAATTACTTAGCTTCGTCGTTTTTc                                                                                                                                                  >  1:479306‑M2/127‑250 (MQ=255)                                                                                                   caaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCAttt                                                                                                                                              <  1:225613‑M2/128‑1 (MQ=255)                                                                                                     accagcaaacgggcattgtgttctgaaatccnggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCg                                                                                                                                            <  2:234084‑M2/130‑1 (MQ=255)                                                                                                          caaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCAt                                                                                                                                       <  2:40801‑M2/135‑1 (MQ=255)                                                                                                           aaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAAGCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATa                                                                                                                                      <  1:445515‑M2/136‑1 (MQ=255)                                                                                                            aacgggcattgtgttctgaaatccaggctattgagtcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATGACTTCGCTTCGCCGGTTTTCATTTCGCCCATAg                                                                                                                                     <  2:387900‑M2/137‑1 (MQ=255)                                                                                                               gggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggactggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCATCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCtt                                                                                                                                  >  1:23089‑M2/112‑251 (MQ=255)                                                                                                                      gtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCtt                                                                                                                           >  2:184415‑M2/105‑251 (MQ=255)                                                                                                                      gtgttctgaaatccaggcgattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCtt                                                                                                                           <  1:487430‑M2/147‑1 (MQ=255)                                                                                                                         ttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCttttt                                                                                                                        >  1:92539‑M2/102‑251 (MQ=255)                                                                                                                             gaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTgg                                                                                                                      >  1:198462‑M2/98‑249 (MQ=255)                                                                                                                               aatccaggcgattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattaaccggaatggtggcgactatAAGTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGat                                                                                                                  <  2:272193‑M2/156‑1 (MQ=255)                                                                                                                                    aggctattgattgaacggcagcgacagatctgcgctgtaaaagaacttgtaaaacatcgtaatagaagtatccggaatggtggcgactatAATTTCGAGTGGTTCTCGGATGAGGATGATGACGGTGATACGCAGATAAGAATCAATTAGCAATGAGGGTTGCCGGGCCACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCATTTTTCATTTCTCCCCTAGCTTTCAGCTTTTTGGAGATAgcgc                                                                                                             <  2:276552‑M2/161‑1 (MQ=255)                                                                                                                                        tattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCgcggcg                                                                                                         >  2:136145‑M2/87‑251 (MQ=255)                                                                                                                                               tcaacgtcagcgacagatctgggctgtaaaagaacgtgtaaaacaacgtaatagaattatccggaagggtggcgactatAATGTCGATTGGTTCTAGGATGAGGATGATGACGGTGATACAGAGATAAGAACCAAGGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAAGTACTTCGCTTCGCCGTTTTTCATTGCGCCCATAGCTGTCAGCTTTTTGGAGATATCGCGGCGTTCttt                                                                                                   <  2:28059‑M2/171‑1 (MQ=255)                                                                                                                                               tcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCttt                                                                                                   <  2:272194‑M2/171‑1 (MQ=255)                                                                                                                                                    gtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactctAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGgag                                                                                               >  1:166820‑M2/75‑249 (MQ=255)                                                                                                                                                          gacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGCAACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAAAGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAAATATCGCGGCGATCTTTGGAGAGCTCgg                                                                                        >  2:393457‑M2/69‑250 (MQ=255)                                                                                                                                                             agatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGNTATCGCGGCGTTCTTTGGAGAGCTCGGCAtt                                                                                    >  2:225491‑M2/66‑251 (MQ=255)                                                                                                                                                                           aaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAAt                                                                      <  2:49184‑M2/200‑1 (MQ=255)                                                                                                                                                                               aacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTc                                                                  <  1:219303‑M2/204‑1 (MQ=255)                                                                                                                                                                                acttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCAGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTc                                                                  >  2:375318‑M2/47‑250 (MQ=255)                                                                                                                                                                                    gtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAAcgc                                                             >  1:261073‑M2/43‑251 (MQ=255)                                                                                                                                                                                        aacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATc                                                         >  1:324395‑M2/39‑251 (MQ=255)                                                                                                                                                                                              gtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTtgat                                                                              <  1:279113‑M2/192‑1 (MQ=255)                                                                                                                                                                                              gtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTtgat                                                                              >  2:279113‑M2/33‑224 (MQ=255)                                                                                                                                                                                                 atagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGCTGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTc                                                >  2:286558‑M2/30‑251 (MQ=255)                                                                                                                                                                                                    gaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCAcc                                             <  1:184620‑M2/225‑1 (MQ=255)                                                                                                                                                                                                         atccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTc                                         <  1:184415‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                atggtggcgactatAATTTCGATTGGTTATCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGc                                 >  1:321389‑M2/15‑251 (MQ=255)                                                                                                                                                                                                                                       tGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCa           <  2:150856/250‑1 (MQ=255)                                                                                                                                                                                                                                             tcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATAAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTc     >  1:267137/1‑250 (MQ=255)                                                                                                                                                                                                                                               ggatgaggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATc  <  1:331293/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTCATC  >  NC_000913/2078709‑2079197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑