Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,249	A→T	intergenic (+1088/+10)	yafP → / ← pepD	putative N‑acetyltransferase YafP/peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,249	0	A	T	100.0%	201.6 / NA	61	intergenic (+1088/+10)	yafP/pepD	putative N‑acetyltransferase YafP/peptidase D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/34);  total (27/34)

TATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCA  >  NC_000913/254004‑254494                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGcatatca                                                                                                                                                                                                                                                   >  1:195701/1‑250 (MQ=255)           aaGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACtt                                                                                                                                                                                                                                        <  1:345703/251‑1 (MQ=255)                    gcagAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAAt                                                                                                                                                                                                                               <  1:175094/250‑1 (MQ=255)                      agagCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAACTAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAAttt                                                                                                                                                                                                                             >  2:418109/1‑251 (MQ=255)                           gTAGTCAGCATGCTAACCAGCGGCTCGCACGATTGCTGCTTGCCTGGAAGCTGGAGCAACAGCAAAAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACCGGGATGGCTTTTATCGAAGGATAATAACGGTTGAAACTGCCGGAAGTCGATTAAACGCCACCCGCCAGCANATCAAATAATTACATCGCCGGACtttctttc                                                                                                                                                                                                                        >  2:214738/1‑251 (MQ=255)                                 aGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCCTCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcagg                                                                                                                                                                                                                  >  2:368922/1‑250 (MQ=255)                                 aGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGt                                                                                                                                                                                                                  >  1:481742/1‑251 (MQ=255)                                      gccaaCAAGCGGCTGGCACGATGGCTGATTTCCTGGAAGCGGGAGCAACAGCAACAGGAAATGAGCGCGGCGCTGAAATCGCAGCGGCGAATGTGCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATTGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTGTCAGCAGTtcag                                                                                                                                                                                                              <  2:41052/247‑1 (MQ=255)                                         accAAGCGGCTGTCCCGTTTGCTGATTGCATGGAAGCGGGAGAAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGAGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGGCGGACATTTACAGGGATGGCTTTTATCGAAGGCTAATGAAGGATGCAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtcag                                                                                                                                                                                                          <  2:138303/249‑1 (MQ=255)                                         aaCAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagtc                                                                                                                                                                                                            <  2:80134/249‑1 (MQ=255)                                                       aCGATTGCTGATTGCCTGTAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCAATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGGGTCCAGTAAt                                                                                                                                                                                            >  2:402974/1‑251 (MQ=255)                                                          aTTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGAc                                                                                                                                                                                         >  2:428602/1‑251 (MQ=255)                                                             gCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGAGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAAATGCCGGAAGGCGATTAAAAGCCATCCGGCATCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCt                                                                                                                                                                                       >  1:460249/1‑250 (MQ=255)                                                                      cTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCg                                                                                                                                                                              >  2:396575/1‑250 (MQ=255)                                                                           aGCTGGAGCACCAGCAACAGGAAACTAGAGCGCCGCTGAAATCGCAGCGGCGAATGTTCCATAACCAGATTGCACGTGGCAACCCGCGACGGACATCTACAGGGATGGCTTTTATCGAAGGATAATGAAGAATGAAACTGCCGGAAGGCGATTAAACACCATCCGGCAGCATCACAAATAATTACTTCGCCGGAATTTCTTTCAACAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtgtg                                                                                                                                                                         >  2:393095/1‑250 (MQ=255)                                                                             cTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                                                                                                                                      >  1:35987/1‑251 (MQ=255)                                                                               ggAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACt                                                                                                                                                                     <  1:244718/250‑1 (MQ=255)                                                                                gAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTg                                                                                                                                                                   <  2:343839/251‑1 (MQ=255)                                                                                 agcaacagcaacAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGc                                                                                                                                                                  >  1:251241/1‑251 (MQ=255)                                                                                  gcaacagcaacaGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCTATTAAACGCCATCCGGCAGCATATCAAATAATTTCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCt                                                                                                                                                                 <  1:235728/251‑1 (MQ=255)                                                                                   caacagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGAAGGAAATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAACTAATTAATTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGACGTGTCCAGCAATGACCTACGCTTTCGATGTGAACTTGCTc                                                                                                                                                                >  2:488315/1‑251 (MQ=255)                                                                                       agcaacagGAACAGAGCGTGGCGCGGACATAGCAGCGGCCAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGAGGGCTTTTATCGAAGGACAATGAAGGATGCAACTTCCGGAAAGCGATTAAACGCCATCCGGCAGCATATCAAATAATTGCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCATCAGTGTCCAGTAATGTCCTACGCTTTCGATGTGAACTTGCTCATcc                                                                                                                                                            <  2:88813/251‑1 (MQ=255)                                                                                          aacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGga                                                                                                                                                         <  1:145510/251‑1 (MQ=255)                                                                                                aaaaTAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGACCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAgtgt                                                                                                                                                   <  1:268524/251‑1 (MQ=255)                                                                                                     aGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAcc                                                                                                                                              <  1:31412/251‑1 (MQ=255)                                                                                                              atGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAAACCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGt                                                                                                                                     >  2:430182/2‑251 (MQ=255)                                                                                                                     cGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                                                                                                                               <  1:14291/250‑1 (MQ=255)                                                                                                                          cggcgAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTAGCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTGCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGa                                                                                                                         <  2:209490/251‑1 (MQ=255)                                                                                                                                attttCCATCACCAGATTGAACGTGGCAACCNGCGACGGACATTTACAGGGGTGGCTTGTATCGAAGGATAAGGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCAt                                                                                                                   <  2:222391/248‑1 (MQ=255)                                                                                                                                   ttCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGAGATAGTTGGCCCGATAGAAACCATGTc                                                                                                                >  1:203022/1‑251 (MQ=255)                                                                                                                                     ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTcc                                                                                                               >  1:226264/1‑250 (MQ=255)                                                                                                                                          aCCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGAGAATGAAGGATGAAACTTGCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTGTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTcc                                                                                                         <  2:268442/251‑1 (MQ=255)                                                                                                                                              gATTGAACGTGGCAAACCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGAt                                                                                                     <  2:469107/251‑1 (MQ=255)                                                                                                                                                      gTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttt                                                                                             >  1:204674/1‑251 (MQ=255)                                                                                                                                                      gTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGtttt                                                                                             <  1:2935/251‑1 (MQ=255)                                                                                                                                                               cgcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAAc                                                                                       >  1:251385/1‑248 (MQ=255)                                                                                                                                                                 ggACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGCCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAcc                                                                                  <  1:418109/250‑1 (MQ=255)                                                                                                                                                                  gACGGACATTTACAGGGATGGCTTTTATCGAAGGACAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGAcc                                                                                  >  2:145031/1‑250 (MQ=255)                                                                                                                                                                    cGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACcac                                                                                >  2:392247/1‑250 (MQ=255)                                                                                                                                                                              cAGGGATGGCTTTTAACGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGccc                                                                     <  2:481742/251‑1 (MQ=255)                                                                                                                                                                                                 aGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGt                                                  <  2:345952/251‑1 (MQ=255)                                                                                                                                                                                                   gATAATGAAGGATGAAACTGCCTGAAGGCGANTAAACGCCATCCGGCAGCATAGCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTc                                                <  2:242477/251‑1 (MQ=255)                                                                                                                                                                                                   gATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTc                                                <  1:354383/251‑1 (MQ=255)                                                                                                                                                                                                   gATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTc                                                <  1:104358/251‑1 (MQ=255)                                                                                                                                                                                                       aTGAAGGTGGAAACAGCCGGATGGCGATTAAACGCCAGCCGGCCGCATATCAAATAATTACTGCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGCGAACTTGCTCAGCCGGAGAGTGTGGAACGGTGATAGTTGGCCCGATAGAAACCATGGCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTCCGGCg                                            <  2:466182/251‑1 (MQ=255)                                                                                                                                                                                                       aTGAAGGATGAATCTTCCGGAAGGCGATTAAACGCCAACCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGAGTCCAGTAATGACTTACGCTTTCGGTGGGAACTTGCTCATCCGGAGAGTGTGGACCGGTGCTAGTTGGCCCGATAGAAACCATGTCAATTTACGGATACGGTTTTTTGAGCAGACCACATTCCAAGCCCGCGTTGATAATCTGGATGTTTGGCg                                            >  2:466941/1‑251 (MQ=255)                                                                                                                                                                                                       aTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCg                                            <  1:52905/251‑1 (MQ=255)                                                                                                                                                                                                           aGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCtt                                        <  1:266306/251‑1 (MQ=255)                                                                                                                                                                                                              aTGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATAACAAATAATTGCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGTCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgtt                                     <  2:203022/251‑1 (MQ=255)                                                                                                                                                                                                                gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                    >  1:363568/1‑250 (MQ=255)                                                                                                                                                                                                                      gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGTAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAGCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGTATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                              <  1:367771/250‑1 (MQ=255)                                                                                                                                                                                                                           aagtcGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGgcgc                           >  1:255812/5‑248 (MQ=255)                                                                                                                                                                                                                           aaGGCGATTAAACGCCATCCGGCAGCATTTCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAAATTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATCCGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACCGGAGCTg                         >  2:296200/1‑250 (MQ=255)                                                                                                                                                                                                                            aGGCGATTAAACCCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGGCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGTACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTGTTTTGAACAGACCACATTCCAGGCCCGCTTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAt                       <  2:372176/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTTAAATGCCATCCCGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGCCCTACGCTTTCGATGTGAACTTTCTCATCCGGAGAGTGTGGCCCGGTGATAGTTGGCCCGATAGAAACCATGGCCATTTCCGGATACGGTTTTTTGAACAGCCCACATTCCAGGCCCGCGTGGATAATATGGATGTTCGGAGTCTTGTTGAACAGGCGCTGATAGGtt                  >  2:397336/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaaCGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGACTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGCTGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGTATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTc                >  2:209054/1‑250 (MQ=255)                                                                                                                                                                                                                                       cGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCGGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGt            <  2:35987/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTATTTCAGCAGTTCAGTCAGCAGAGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGTTAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGt            >  2:269151/1‑250 (MQ=255)                                                                                                                                                                                                                                         ccATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCACGACCATTTACGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGCTAATCTGGATGTTAGGCGTCTTGTTGAACAGGCGCTGATAGGCTTCACGTAc          >  2:91843/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACAACATTCCAGGCCCGCGTGGATACTCTGGATGTTCGGAGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAca         >  2:9701/1‑249 (MQ=255)                                                                                                                                                                                                                                               ggCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATg    <  1:428602/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATg    <  1:368922/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cagcaTATAAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCa  <  2:376298/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TATTAGCGTGAAGGTTCAGTCAGAGCGTAGTCAGCATGCTAACAAGCGGCTGGCACGATTGCTGATTGCCTGGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCA  >  NC_000913/254004‑254494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑