Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,887,644	C→T	W116* (TGG→TGA)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,887,644	0	C	T	100.0%	203.9 / NA	63	W116* (TGG→TGA)	rnd	RNase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

CATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAACCCCAGCTGCGGGTAATAA  >  NC_000913/1887397‑1887884                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 cATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCAt                                                                                                                                                                                                                                               >  1:105347/1‑251 (MQ=255)  aTCTTCCGGCGCAACGACTTCCTGACGCCGCATTTGCATCAGGCGGCATACATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTTGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATTGCAGCGAAACCTCAtt                                                                                                                                                                                                                                              >  1:266637/1‑250 (MQ=255)  aTCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGAAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCAGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCCGGCCAGCCAGTCGGTGCGCGATTCAATCTTGTCCAGCGTAACGCAGGAATACTCTTCCACCATGGAAGCGAAACCTCATg                                                                                                                                                                                                                                              >  1:437116/1‑251 (MQ=255)      tCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCCCCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTCCCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACAGCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGAc                                                                                                                                                                                                                                            <  2:253225/249‑1 (MQ=255)       ccGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCAAGGAAGCGAAACCTCATGACATc                                                                                                                                                                                                                                         >  1:218683/1‑251 (MQ=255)            gcAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCg                                                                                                                                                                                                                                    <  1:126729/251‑1 (MQ=255)             cAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt                                                                                                                                                                                                                                   <  1:272177/251‑1 (MQ=255)                      ccTGACGACGCATTTGCATCAGGCGGCATTCATCCAGAGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATACGCCGCTGCGGATTCACACTGACGTTCGGGCAGCGGTCTGGCCCGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACCCCGGCATACTCCTCCACCATGGAAGCGAAAACTCATGACATAGGGCGTCCGccga                                                                                                                                                                                                                            >  2:416256/1‑246 (MQ=255)                        tGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAAACTCATGACATCGGGCGTCCGCAGAAGgc                                                                                                                                                                                                                        >  1:181352/1‑251 (MQ=255)                         gacgacGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGGGATCGGGAACAGATACCAGACATCCGACGCTGCGTATTCACACTGACGTTCGGTCAGCGGGCTGGCCAGCCAGACGGTGCGCGATTCACTCTTGTCCAGCGTAACCCCGGAATACTCTTCCACCATGGAAGCGAAACCTCCTGACATCGGGCGTCCGCAGAAGgcc                                                                                                                                                                                                                       >  2:285322/1‑250 (MQ=255)                          ccgacgCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCCCACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGgcag                                                                                                                                                                                                                      <  2:169301/250‑1 (MQ=255)                                              ggcATTCATCCAGCGCCGCAGTGAGCCAGCCGGAGGCCCCCGTTTCTACCATAAGCTTGGCGGGGATCGGTAACAGATACCAGACATCCGCCGCTGCTTATTCACACTGACTTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCTCCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc                                                                                                                                                                                                  <  1:27001/251‑1 (MQ=255)                                                 aTTCATCCAGCGCCCGAGGTGGCCAGCCGGAGGCCTCCTTTCCTACCATAAGTTGGGCGGTGATCTGTAACAGATACCAGACCTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAg                                                                                                                                                                                               <  1:88980/251‑1 (MQ=255)                                                  ttCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAgg                                                                                                                                                                                              >  1:261645/1‑251 (MQ=255)                                                    cATCCAGCGCCGCAGGGAGCGAGCCGGAGTCCTCCGTTTCTACCATAAGCTGTGCGGTGACCGGTAACAGATCCCAGGCATCCGCCGCTGCGTATTCACACTGACGTGCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGt                                                                                                                                                                                            <  2:263507/251‑1 (MQ=255)                                                     aTCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTTGGCGGTAACAGATACCAGACAACCTCCGCTGCGAATTCACACTGACGATCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGCTTCACTCTTGGCCAGCGTAACGCCGGAATACTCTTCCACCACGGAAGCGAAACCTCATGACATCGGGCGTCCTCAGAGGGGACCAAGGCTTTGCGGGTCAAGCAAggggt                                                                                                                                                                                           >  2:19673/1‑249 (MQ=255)                                                            gccgcAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGATCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCCATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGCAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAAGATTTGCGTGTCAATCAAGGGTTGTggaa                                                                                                                                                                                     >  2:86000/1‑248 (MQ=255)                                                                         agccGAAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGGTGTGGTAATTCGCCAAAGAc                                                                                                                                                                        >  1:144871/1‑250 (MQ=255)                                                                                ggCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTCACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGCTTTGCGTGTCAATCAAGGGTTGGGGGAATTCGCCAAAGACATTGAgg                                                                                                                                                                 >  2:164146/1‑250 (MQ=255)                                                                                   cTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTCACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTTCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGGAGCAAGGATTTGAGTGTCAATCAAGGGTTGGGGTAATTCGCCAAAGACATTGAGGAac                                                                                                                                                              >  1:120530/1‑250 (MQ=255)                                                                                          tCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCAATCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGGACACTTcc                                                                                                                                                        >  2:371476/1‑249 (MQ=255)                                                                                             aCCATAAGCTTCGCGGTGATCGGTAACAGAGACCAGACATCCGCCGCTGCGTATTCACAATGACGTTCGGTCAGCGGTCAGGCCAGACAGTCGGCGCGCGATTCACTCTTGTCCCGCGTAACGCCGGAATACTCTTCAATCATGGAAGCGAAACCTCCTGAAATCGGGAGTCCGCAAAAAGCAGCAAGGATTAGCGTGTCAATCACGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTcc                                                                                                                                                        >  2:286346/1‑246 (MQ=255)                                                                                              ccATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCCAAGACATTGAGGAACACTTCCAGATCt                                                                                                                                                  >  2:82606/1‑251 (MQ=255)                                                                                               cATAGGCTGGGCGGTGATCGGTAACAGATCCCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCtt                                                                                                                                                 <  2:45494/251‑1 (MQ=255)                                                                                                aTAAGCTTGGCGGTGATCGGGAACCGATACCAGACATCCGCCGCGGCGTCTTCACGCTGACGCTCGGTCAGGGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGCATAAGCTTCCACCATGGAAGCGAAAACTCATGGCATCGGGCGTCCGCAGAAAGCAGCAAGGATTTGCGTGTCAATCACGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCAAGacattg                                                                                                                                                >  2:140153/1‑246 (MQ=255)                                                                                                       gggCGGTGTTCGGTAACAGATACCAGCCTTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCActgcct                                                                                                                                         <  2:478453/250‑1 (MQ=255)                                                                                                       gggCGGTGTGCGGTAACAGTTACCATACATCCGGCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGGCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAACCCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACAGTGAGGAACACTTCCAGATCTTCActgcc                                                                                                                                          <  2:259008/249‑1 (MQ=255)                                                                                                            gggcccGGTAACACACATCAGACATCCGCCGCTGCGTATTAACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTTGGTGCGCGATTCACTCTTGGCCAGCGTAACGCCGGAATACTCGTCCACCATGGAAGCGAGACCTCATGACATCGGGAGGCCGCAGAAGGCAGCAAGGATTTGCTTGTCAATCAAGGGTGGTGGTAATTCGCCAAAGACATTGAGGAGCCCTTCCAGATGTGCACTGCCTGTATg                                                                                                                                    <  2:305490/246‑1 (MQ=255)                                                                                                               aTCGGTAAAAGATCCCAGGCCTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATgtag                                                                                                                                 <  2:91765/251‑4 (MQ=255)                                                                                                                gcagTAACAGATACAAGACCCCCGCCGCTGCGTATTCACGCTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACTATGGAAGCGTAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGTCATTGATGAACTCTTCCAGATCTTCACTGCCTGCATGgaga                                                                                                                                <  1:379397/248‑1 (MQ=255)                                                                                                                 cggtaccagagcccggccttcccccgcGGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCGTCACTGCCTGCATGgaga                                                                                                                                <  2:273184/227‑1 (MQ=255)                                                                                                                 cGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGgaga                                                                                                                                >  1:12052/1‑250 (MQ=255)                                                                                                                       cAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTtg                                                                                                                         >  2:483232/1‑251 (MQ=255)                                                                                                                        aGATCCCAGACCTCCGCCGCTGCGTATTCACCCTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTtgt                                                                                                                        <  2:131888/251‑1 (MQ=255)                                                                                                                        aGATCCCAGACACCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTtgt                                                                                                                        <  2:261645/251‑1 (MQ=255)                                                                                                                          aTACCCGACATCCGCCGCTGCGTATTCACACTGAAGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTACCGCCGGAATACTCTTCCACCATGGAAGCGACACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGCTTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTtgtg                                                                                                                       >  2:283004/1‑250 (MQ=255)                                                                                                                               agccttCCGCCGCGGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGAc                                                                                                                 <  2:430656/246‑1 (MQ=255)                                                                                                                                gACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACg                                                                                                                >  1:449311/1‑251 (MQ=255)                                                                                                                                   aTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGAt                                                                                                             >  1:6182/1‑251 (MQ=255)                                                                                                                                        ccgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCAt                                                                                                                                     >  2:390721/1‑222 (MQ=255)                                                                                                                                        ccgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCAt                                                                                                                                     <  1:390721/222‑1 (MQ=255)                                                                                                                                              cGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATc                                                                                                  <  1:19673/251‑1 (MQ=255)                                                                                                                                                tATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGc                                                                                                >  2:70644/1‑251 (MQ=255)                                                                                                                                                 aTTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCt                                                                                               >  1:87752/1‑251 (MQ=255)                                                                                                                                                  ttCACACTGTCGTCCGATCTGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCAACATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAAGTCGCCAAAGCCATTGATGAACACTTCCAGGTCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCt                                                                                               <  2:83493/250‑1 (MQ=255)                                                                                                                                                             gTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGAc                                                                                   >  2:363523/1‑251 (MQ=255)                                                                                                                                                                cGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCa                                                                                 <  1:435537/250‑1 (MQ=255)                                                                                                                                                                     aGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGGGACCCGTCGGt                                                                           >  2:449523/1‑251 (MQ=255)                                                                                                                                                                      gCGGTCTGGCCAGCCAGTCGGTGCGCGAGTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGTAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGGCGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTg                                                                          <  1:177545/251‑1 (MQ=255)                                                                                                                                                                          tCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGCTCGCTTTCAGCGGTGACCAGTCGGTGATcc                                                                      >  2:55060/1‑251 (MQ=255)                                                                                                                                                                               ccagccagTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGt                                                                 <  1:458964/251‑1 (MQ=255)                                                                                                                                                                                cagccagTCGGTGCGCGATTCACTCTTGTCCAGCATAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCCCTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGGGATCCCGAGTg                                                                >  2:347002/1‑251 (MQ=255)                                                                                                                                                                                 agccagTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTgg                                                               <  1:464951/251‑1 (MQ=255)                                                                                                                                                                                            gcgcGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAgc                                                     <  1:372834/250‑1 (MQ=255)                                                                                                                                                                                                   tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCTCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGTACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGAt                                              <  1:286346/250‑1 (MQ=255)                                                                                                                                                                                                          tGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATGTTTTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGGGATCCCGAGTGGATCGAGGAGCGCCAGAGGCTCGcc                                       <  2:411652/250‑1 (MQ=255)                                                                                                                                                                                                            gccAGCGTAACGCCGGAATCCTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATc                                    <  2:105347/250‑1 (MQ=255)                                                                                                                                                                                                                  gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGGCCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCACGcccacgaaa                                >  2:398158/1‑242 (MQ=255)                                                                                                                                                                                                                             aTACTCTTCCACCATGGAAGCTAAACCTCATGACATCGGGCGGCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACCCTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGTATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAAcc                   <  2:6182/251‑1 (MQ=255)                                                                                                                                                                                                                               aCTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAAcccc                 >  1:47868/1‑251 (MQ=255)                                                                                                                                                                                                                                ctctTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAAcccc                 >  1:297928/1‑250 (MQ=255)                                                                                                                                                                                                                                            ggAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAACCCCAGCTGCGGGtaat    <  1:354178/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaGCGAAACCTCATGACAACGGGTGTCCGCAGAAGGCAGCAAGGATTTGCTTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGTCATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGAGGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGTGCCAGATGCTCGCCATCGAAAAGTTGAATCAACCCCAGCTGCGGGtaataa  <  2:422487/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCATCCAGCGCCGCAGGTAGCCAGCCGGAGGCCTCCGTTTCTACCATAAGCTTGGCGGTGATCGGTAACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCAAAGACATTGAGGAACACTTCCAGATCTTCACTGCCTGCATGGAGAAATTTTGTGATGGACGGATCGCGCAGGATCGCTTTCAGCGGTGACCAGTCGGTGATCCCGAGTGGATCGATTAGCGCCAGATGCTCGCCATCGAAAAGTTGAATCAACCCCAGCTGCGGGTAATAA  >  NC_000913/1887397‑1887884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑