Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,851,841	C→T	Q328* (CAA→TAA)	ansA →	L‑asparaginase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,851,841	0	C	T	98.1%	184.9 / NA	52	Q328* (CAA→TAA)	ansA	L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/30);  minor base G (1/0);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTA  >  NC_000913/1851596‑1852087                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aTGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAAc                                                                                                                                                                                                                                                   <  1:51996/251‑1 (MQ=255)   gcgcCACAAATCAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGTTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTTAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCAGTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCt                                                                                                                                                                                                                                                 <  2:299352/251‑1 (MQ=255)   gcgcCACAAAACAAATCTTTCCTTCAAGAANTACAAGAAGCCAGCGATCGCGGTATTTGGGGGGTCAACCTGGCACAATGTATTTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTTGATACTGAAACCATTCGCAAGGCCATGAGCTAAAAcc                                                                                                                                                                                                                                                  <  2:248832/250‑1 (MQ=255)   gcgcCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAAcc                                                                                                                                                                                                                                                  <  1:160308/250‑1 (MQ=255)       cacaAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTgcgc                                                                                                                                                                                                                                             <  1:434022/251‑1 (MQ=255)        ccaaaacaaaGCCTACCTGCAGGAATTACAAGAAGCCAGCGAGCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCGCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTTCCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGACATGAGCTAAAACCTGcgcg                                                                                                                                                                                                                                            <  2:37903/250‑1 (MQ=255)                   ccTTCCTGCAGGAATTACAAGAAGCCAGCGAGCGCGTTATTGTGGAGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACg                                                                                                                                                                                                                                 <  2:453862/251‑1 (MQ=255)                        cTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGCGATAAAACCTGCGCGGCGAACTGAAGCtttg                                                                                                                                                                                                                            >  2:440666‑M1/1‑247 (MQ=255)                          gCAGGAATTACAAGAAGCCAGCGAGCGCGGTATTGTGGTGGTCAACCTGACACAATGTATTGCCGTTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgc                                                                                                                                                                                                                           <  2:469078‑M1/250‑6 (MQ=255)                             ggAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcac                                                                                                                                                                                                                       <  1:198507‑M1/251‑10 (MQ=255)                                              gCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtg                                                                                                                                                                                                      <  1:440666‑M1/251‑27 (MQ=255)                                               cGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgc                                                                                                                                                                                                     <  2:371772‑M1/251‑28 (MQ=255)                                               cGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgc                                                                                                                                                                                                     >  1:102733‑M1/1‑224 (MQ=255)                                                gATCGCGGTATTGTGGGGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGGGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATAATGAAACCCTTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTTNCGCtttgctcaactcccactttaaccgtgcc                                                                                                                                                                                                    >  2:233142‑M1/1‑223 (MQ=255)                                                 aTCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcac                                                                                                                                                                                                                >  1:134911‑M1/1‑222 (MQ=255)                                                 aTCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGGCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcac                                                                                                                                                                                                                <  2:134911‑M1/238‑17 (MQ=255)                                                            gtggtgGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtga                                                                                                                                                                                        <  1:433012‑M1/251‑41 (MQ=255)                                                             gggtggtcAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgac                                                                                                                                                                                       <  2:145961‑M1/250‑42 (MQ=255)                                                                    cAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattct                                                                                                                                                                                <  2:131136‑M1/251‑49 (MQ=255)                                                                       ccTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGCACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgggactgattctaac                                                                                                                                                                             >  2:465138‑M1/1‑200 (MQ=255)                                                                          gACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaa                                                                                                                                                                           >  1:42557‑M1/1‑197 (MQ=255)                                                                               aaTGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatcccactttaatcgtgctcacattacgtgactgattctaacaaaacatt                                                                                                                                                                     >  2:34922‑M1/1‑192 (MQ=255)                                                                                         cGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaact                                                                                                                                                           >  1:221504‑M1/1‑182 (MQ=255)                                                                                                  gAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaat                                                                                                                                                   <  1:465138‑M1/250‑78 (MQ=255)                                                                                                    aCATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaatttt                                                                                                                                                >  1:264119‑M1/1‑171 (MQ=255)                                                                                                    aCATGGGTGGTTATGCCACCGGTAACGCCCGCGCCCAGGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaatttt                                                                                                                                                <  2:88823‑M1/251‑81 (MQ=255)                                                                                                     cATGTGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCAGTACCGGCTTAGCCAGGAACTGGATTCTGAATCCATGCGCAAGGCCATGAGCTAAAACCGGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttg                                                                                                                                               <  1:460466‑M1/251‑82 (MQ=255)                                                                                                      aTGGGTGGTTATGCCCCCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgt                                                                                                                                              <  1:52133‑M1/251‑83 (MQ=255)                                                                                                       tGGGTGGTTATGCCACCGGTAACGCCCTCTCCCATGCCGGCGTAATTGGCGGGGCAGATATGACTGTAGCCTCCACGCTAACCAATCTGCATTACCTGCTTAGCCAGGAACTGGCTACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTTCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcccattacttgactgattctaccaaaccattaccaccacctggcaaccttttgtc                                                                                                                                             >  2:55547‑M1/1‑168 (MQ=255)                                                                                                                     aCCGGTAACGCCCTCGCCAATGCCGGCGTAATTGGCGGCGCAGGAATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTTAAACAATTCGCAAGGCCATGCGCGAAAAACTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgataccattacgcgacttattctaacaaaacattaacaccaactggcaacagtttgtcctaaacttgatctc                                                                                                                               >  2:187277‑M1/1‑154 (MQ=255)                                                                                                                        ggTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgaatgattctaacaaaaccttaacaccaacctgcaaaattttgtcctaaacttgatctcgac                                                                                                                            >  2:447926‑M1/1‑151 (MQ=255)                                                                                                                                   cGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgc                                                                                                                 <  1:259982‑M1/251‑112 (MQ=255)                                                                                                                                   cGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggct                                                                                                                   <  1:447057‑M1/249‑110 (MQ=255)                                                                                                                                        aTGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcaccta                                                                                                            <  2:114685‑M1/251‑117 (MQ=255)                                                                                                                                             ggCGTAAGTGGCGGTGCAGATATGACTGTAGAAGCAACGCTAACCAAACTGGATTGCCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATTAGCTAAAACCTGCGCGGCGAACTGTCGCtttgctaacatctcactttaaaagtgctctcattacgtgactgattctaacaaaacattatcacctactggcaaaattgtgtcctaaacttgagctcaacgaattggctgcacctaaatct                                                                                                       <  2:422681‑M1/251‑122 (MQ=255)                                                                                                                                                gTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGAAAGGCCATGAGCTAAAACCCGCGCGGCGAAATGACGCtttgcgcaaatctcactttaatcgtgctcacattaggtgactgattctaacaaaaaattaacaccaacagacaaaaatttgtcctaaacttgatctcgccgaaatggctgccccgaaattgagg                                                                                                    >  2:92732‑M1/1‑127 (MQ=255)                                                                                                                                                                    gACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGCACTGGATACTGAAAACATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctaactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcg                                                                                >  2:423231‑M1/1‑107 (MQ=255)                                                                                                                                                                        gTAGAAGCCACGCTATCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAGCCTGCGCGGCTAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacgtgatctcgacgaaatggctgaacctaaatcgtgatgaaaatcacatttttatcgtaat                                                                            <  2:430600‑M1/251‑149 (MQ=255)                                                                                                                                                                         tAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACACTTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaatt                                                                           >  2:67486‑M1/1‑102 (MQ=255)                                                                                                                                                                               ccACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgcgcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaatcgccctt                                                                     <  1:290813‑M1/251‑156 (MQ=255)                                                                                                                                                                                    cTAACCAAACTGCATTACATGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaa                                                                 >  2:142318‑M1/1‑91 (MQ=255)                                                                                                                                                                                      aaCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTTACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaat                                                               <  1:58801‑M1/250‑162 (MQ=255)                                                                                                                                                                                      aaCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaacattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcaaatttttatcgtaattgccctttaaaatt                                                              >  2:173947‑M1/1‑89 (MQ=255)                                                                                                                                                                                         cAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcgg                                                           <  1:440504‑M1/251‑166 (MQ=255)                                                                                                                                                                                                   tACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacctgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgac                                                  <  1:447926‑M1/250‑175 (MQ=255)                                                                                                                                                                                                                  gAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctca                                    >  2:177479‑M1/1‑61 (MQ=255)                                                                                                                                                                                                                    aCTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcgaggcgccgaccccatgtggtctcaagcc                                >  1:228414‑M1/1‑59 (MQ=255)                                                                                                                                                                                                                        gATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgcttctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgcagaccccatgtggtctcaagcccaa                             >  2:193907‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                                   cATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagtgag                 <  1:34922‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                                          aaGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagtgaggcgagtc          >  2:412522‑M1/1‑37 (MQ=255)                                                                                                                                                                                                                                          aaGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagtgaggcgagtc          >  2:86635‑M1/1‑37 (MQ=255)                                                                                                                                                                                                                                                  gAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagtgaggcgagtcagtcgcgt  <  2:3784‑M1/251‑223 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTA  >  NC_000913/1851596‑1852087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑