Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,638,571	G→A	H33Y (CAT→TAT)	cspI ←	cold shock‑like protein CspI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,638,571	0	G	A	100.0%	164.7 / NA	47	H33Y (CAT→TAT)	cspI	cold shock‑like protein CspI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

CATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACAC  >  NC_000913/1638325‑1638817                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       atcaataccctggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACa                                                                                                                                                                                                                                                    <  1:288347‑M2/109‑1 (MQ=255)     ataccctggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAac                                                                                                                                                                                                                                                 >  1:464466‑M2/139‑250 (MQ=255)          ctggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCt                                                                                                                                                                                                                                           >  1:379059‑M2/134‑251 (MQ=255)            ggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCttt                                                                                                                                                                                                                                         <  1:52736‑M2/120‑1 (MQ=255)                   atgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGcc                                                                                                                                                                                                                                   >  1:465670‑M2/125‑250 (MQ=255)                      ccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCACATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGGCAAACACATCTTTGCTGCCATCt                                                                                                                                                                                                                               >  1:214355‑M2/122‑251 (MQ=255)                        ggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCttt                                                                                                                                                                                                                             >  2:302196‑M2/120‑251 (MQ=255)                            accccaatctatgccggactgaatgtcccccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGc                                                                                                                                                                                                                         <  1:47092‑M2/136‑1 (MQ=255)                               caaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGtg                                                                                                                                                                                                                      <  2:26636‑M2/139‑1 (MQ=255)                                        gccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAAcc                                                                                                                                                                                                              >  1:116925‑M2/104‑250 (MQ=255)                                        gccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCa                                                                                                                                                                                                             <  1:47311‑M2/148‑1 (MQ=255)                                           ggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCaaaa                                                                                                                                                                                                          <  2:10960‑M2/151‑1 (MQ=255)                                              atgaaggtcccccttcagtaatttttatacccggggtaattgccgggttaatgcttgtcacaaaaaagtgttagactcatgcagttaaccctctcgcTACAAGAACAGCGGCATGTCCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGCGAAATATACAATCACATCTTTGCTGCCATCTTACGGCGTGATGAAACCAAAAcc                                                                                                                                                                                                        <  2:267127‑M2/153‑1 (MQ=255)                                                      ccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGTGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAgg                                                                                                                                                                                               >  2:341023‑M2/90‑251 (MQ=255)                                                                 aatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAtt                                                                                                                                                                                    <  1:144930‑M2/173‑1 (MQ=255)                                                                  atttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAACTAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                                                                                                                                                   >  1:268897‑M2/78‑251 (MQ=255)                                                                  atttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGTCAAAAACAACTTTGCTGTCATCTTTCGGCGTGATGAATCAAAAACCTTTTTTatggttaaatccttt                                                                                                                                                                                   >  2:131417‑M2/78‑245 (MQ=255)                                                                                   aactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATGCCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAATCCAAATCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAttt                                                                                                                                                                  <  2:83173‑M2/191‑1 (MQ=255)                                                                                   aactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTGGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                                                                                                                                                   <  1:208665‑M2/190‑1 (MQ=255)                                                                                   aactgccgggttattgcttgtcacaaaaaaggggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATAACAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCNGGGTTAAACCATTTCACTAAACCAGTCAttt                                                                                                                                                                  >  2:226952‑M2/61‑251 (MQ=255)                                                                                      tgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt                                                                                                                                                               >  2:292076‑M2/58‑251 (MQ=255)                                                                                                           aaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtttt                                                                                                                                          >  1:446742‑M2/37‑251 (MQ=255)                                                                                                               aagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaag                                                                                                                                      <  2:423994‑M2/219‑1 (MQ=255)                                                                                                                agtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTga                                                                                                                                        >  2:2809‑M2/32‑248 (MQ=255)                                                                                                                   ggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt                                                                                                                                  <  1:292076‑M2/223‑1 (MQ=255)                                                                                                                    gtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt                                                                                                                                 <  1:98501‑M2/224‑1 (MQ=255)                                                                                                                               cagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGc                                                                                                                      <  2:87841‑M2/235‑1 (MQ=255)                                                                                                                                 gttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGc                                                                                                                      >  1:87793‑M2/15‑249 (MQ=255)                                                                                                                                    aacctactcacttcatgtccagtggCAGGACCTTTAGGTCCGTTCTCAATAACAAATTCAACTTCCTGATTCTCAGTTAAAGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAATCACATCTTTGCTGCCATCTTTCTGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAGCCATTTCACTAAACCCGGCTGTTTGTTAGACATAATTATTACCTATTGAAGACAGTAGCCCGTGGGCAGAAt                                                                                                                 <  2:255257/228‑1 (MQ=255)                                                                                                                                                       cAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTACTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATc                                                                                               >  1:379904/1‑250 (MQ=255)                                                                                                                                                                      tAGGTCCGTTCTCAATACCAAATTCAACTGCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGTCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACcaa                                                                                <  1:406038/250‑1 (MQ=255)                                                                                                                                                                         gTCCGTTCTCAATACCAAATTCAACTTCCTGNTTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAg                                                                            <  2:217475/251‑1 (MQ=255)                                                                                                                                                                              ttCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAt                                                                       >  1:102567/1‑251 (MQ=255)                                                                                                                                                                               tctcAATACCAAATTCAACTTCCTGAGTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAt                                                                       <  2:478737/250‑1 (MQ=255)                                                                                                                                                                                  cAATTCCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAAtctc                                                                    <  2:348907/250‑1 (MQ=255)                                                                                                                                                                                          aaTTCAACTTCCTGATTCTCAGATAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAACCTCCTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTAACTTTTGAAGAAATTAGCCCTTGTGCAGAATGGTCCGAAAAAAAATATCATAGAGAAAAACCAACAAGGAAAGCTCCATAGGTat                                                           <  2:465670/251‑2 (MQ=255)                                                                                                                                                                                                        aTTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTTATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATta                                             <  2:74414/251‑1 (MQ=255)                                                                                                                                                                                                        aTTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATta                                             <  1:255728/251‑1 (MQ=255)                                                                                                                                                                                                          tctcAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGACATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACAAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGACGCAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCACCAAGGAAATCTCAAGAGGTACAAATAATAAAATtat                                            >  2:153485/1‑250 (MQ=255)                                                                                                                                                                                                                        tttaaaTCGTTTCTCTGAATGGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTACGTCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATTTTCCGAAAAAAAATATCAGAGAGATAAACCAACAAGGAAATCTCAAGCGTTTCAAATAATAAAATTATAACAATGACTGCtt                              <  2:456024/247‑1 (MQ=255)                                                                                                                                                                                                                         tGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTc                             >  1:199640/1‑250 (MQ=255)                                                                                                                                                                                                                          gAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTc                             >  1:192790/1‑249 (MQ=255)                                                                                                                                                                                                                                       tGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTaa               >  2:432897/1‑250 (MQ=255)                                                                                                                                                                                                                                        gAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAAc              >  1:364689/1‑250 (MQ=255)                                                                                                                                                                                                                                        gAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAAc              >  1:140833/1‑250 (MQ=255)                                                                                                                                                                                                                                        gAATTGCTGAGAAATAGACAAACACATCTTTGCGGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAGTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTTTAACAATGTCTGCTTCAGATAAAGTTGTa                <  2:464466/248‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaTAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTNCTTCAGATAAATTTGTAACAAACCAGAacac  >  2:222296/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACAC  >  NC_000913/1638325‑1638817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑