Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,269,250	A→G	I9T (ATT→ACT)	ldrA ←	small toxic polypeptide LdrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,269,250	0	A	G	100.0%	43.2 / NA	17	I9T (ATT→ACT)	ldrA	small toxic polypeptide LdrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

GGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG  >  NC_000913/1269004‑1269329                                                                                                                                                                                                                                                       |                                                                                ggATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCAt                                                                             >  2:353378/1‑251 (MQ=255)         gCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt                                                                     <  1:221550/251‑1 (MQ=255)                    tttttGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGc                                                             <  2:99160/248‑1 (MQ=255)                      tttGCTTGAAAAATAAAGTATTAGCGTTATGCGTTAAGACTTTTTTCATGGGTGCAGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGGTTCGGGCGCATATTGCCATCCCGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGCCAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCCGGGCGTGCCAGAAAGTCATTGCCAACTgcgacagcggcc                                                         >  2:291507/1‑241 (MQ=17)                             gAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATTGCCAGCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATGGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAgcc                                                  <  1:312740/250‑1 (MQ=255)                                 aataaatgattggCGTTCTGCTTTAGGCTTTNTTTCGGGGGTGACGGAACCAAAAAAGGCCGTGGGCTGTTCCCCCTGCGGCCGTTTTCGGGCCCATATTGCCATCACGGCCGCCTGACGCCCGTTTCCCCCTTACTCCCGGTTACGCCACCAGCGGACAAGCGCGGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCTAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCtt                                             <  2:236319/239‑1 (MQ=2)                                  aTAAAGTATTAGCTTTCGGGGTTAATCCTTTTTTCGTGGGTGCGGGAGAAAAAAAAGGCCGCAGGCTGTCACCCCTGCGGCCGGTTTCGGGCGCAGATTGCCATCCCGGCAGCCTGCCCCCCGTTTTCCCCTTTCGTCCGGTTCCGCCACCAGCTTACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg                                            <  2:287183/251‑1 (MQ=11)                                       gTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTAACGCGGCCAGCCGACAAGCGCTGCAGTAGTGATTCCCGCCGGGGTCGGCGCTTCCCTGACATGCCAGAAAGACTAGGCAAACTGCGCGGGCGTCAGATAgacgcaaggatgt                                        >  2:155695/1‑242 (MQ=12)                                               gTTCTGCGTTAAGTCTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGGTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTCCGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTTTAATGacaac                               <  1:366090/251‑1 (MQ=255)                                                    gCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGAAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCCTCCCGGCAGCCTGACGCCCGTTTTCACCTTCCTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAACCTGCGCGCCCGACCTATAGCCGCCTGGCTGTAATGACGACCtttt                          >  2:476779/1‑251 (MQ=14)                                                     cGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCTGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttgtt                                          >  2:154305/1‑234 (MQ=255)                                                     cGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTGTCGGGCGCATAGTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttgtt                                          <  1:154305/234‑1 (MQ=255)                                                         aaGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATAGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTATTGACAACATTTTGCGGc                     >  2:471360/1‑251 (MQ=255)                                                           gACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAgcc                                                  <  1:311876/220‑1 (MQ=255)                                                           gACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAgcc                                                  >  2:311876/1‑220 (MQ=255)                                                                         gggCCGGATACAAAAAGGGCCGCAGGTTGTTCCCCCTGCGGCCGGTTGCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTGCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTGGAAgtgtct     <  1:323719/249‑3 (MQ=11)                                                                            ccGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGaagtggctggg  >  1:258326/1‑242 (MQ=12)                                                                                                                                                                                                                                                       |                                                                                GGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG  >  NC_000913/1269004‑1269329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑