Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	156.7 / NA	48	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/16);  total (32/16)

TTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCC  >  NC_000913/674274‑674763                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       ttGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCtt                                                                                                                                                                                                                                                  >  1:393187/1‑251 (MQ=255)          cgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCAAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAACGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                          >  1:217417/1‑250 (MQ=255)              aGTACGGTATGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATTTTGGCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCATGCCTTCCGCAGGCAGAcc                                                                                                                                                                                                                                     <  2:407873/251‑1 (MQ=255)                   ggtttGGTCGTCCGGGCACCAGTTGACCGCAGAGGTTTTCTTATATACCAGGCTTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTCCGCCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAAcg                                                                                                                                                                                                                                <  2:3910/247‑1 (MQ=255)                       tGGTCGTTCGGGCACCATTTTACGCCAGAANTCTTCTTATATACCAGGC‑TTTTTT‑GTAACAGCGCGGGAAAAAATTTCGGTCCCCAGCGGAATTATTCCGG‑TGTACGGGTTGCCAGCTTGCGGCTCCAGTCAAAA‑CCAAAGCCCATCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTCCGTCCCGGG‑TGCCGGAGCTGTGTTGTGTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGt                                                                                                                                                                                                                             <  2:230120/250‑1 (MQ=255)                            gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                       >  1:92396/1‑251 (MQ=255)                            gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                       >  2:155006/1‑251 (MQ=255)                                 ggccccAGTTGCCCGCAGATGCTTTCTTA‑CTNCCCGGCCTTTTTTTATC‑CAGCCCGGGAAAGAATTTCGGTTCCCAACGGTGGTTTTCCGG‑CTGACAGGTTCCCACCCCGCGCCTCCAGCCATAA‑CCAAACCCCATCATTTTGGGCTGGTTTTACAAA‑TACGCTAGGTTGACGTACGCCCGCGG‑GGCGGGGGCGGTTTGGTTTTC‑CGCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                  <  2:217417/247‑1 (MQ=255)                                 ggCACCAGTTGACCGTAGAATTCTTCTTATATACCAGGCCTTTTTT‑ATC‑CAGCTCGGTGAAGAATTTCTTTTCCCAACGGTAGTATTCCGG‑CTTACAGGTTGCCAGCTCGCGGCTCCATTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTTTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc                                                                                                                                                                                                                  <  1:318684/251‑1 (MQ=255)                                           gACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAggc                                                                                                                                                                                                        >  1:112358/1‑250 (MQ=255)                                                   aaGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTg                                                                                                                                                                                                >  1:375661/1‑251 (MQ=255)                                                             atatcCCAGGCCTTTTTT‑ATA‑CAGCTGGGTGAAGATTTTCTTTTCCAAACGGTAGAATTCCGG‑CTTACAGGTTGCCACCCCGCGGCTCCAGTCATAC‑CCAAAGCCCAGAATTTTGAGCTGTTTTTCCATA‑TACGCTTTGTTGCCGTACGCCCCCGG‑GGCCGGGGCGGT‑TTGTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGGCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATa                                                                                                                                                                                      <  2:375434/250‑1 (MQ=255)                                                             atataCCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATAc                                                                                                                                                                                     >  1:248905/1‑251 (MQ=255)                                                                  ccAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTgg                                                                                                                                                                                >  1:243210/1‑251 (MQ=255)                                                                  ccAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTgg                                                                                                                                                                                >  1:16944/1‑251 (MQ=255)                                                                    aGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGATGCAGGACGTTTTTGCCCAG‑CATACGCTggg                                                                                                                                                                               >  2:347707/1‑249 (MQ=255)                                                                      cccTTTTTA‑ATA‑CCGCGCGGTGAAAAATTTCTGTCCCCAAGGGTAGTATCCCGG‑CTTACGGGTTGCCAGCTCGCGGCACCATTCATAA‑CCAAAGCCCATCATTTTG‑GGTGGGTTTTCAAAATACGCGTTGTTGTCGGACGCCCACGG‑TGCCGGAGCGGTGTGGTTTTC‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGc                                                                                                                                                                            <  2:112358/250‑1 (MQ=255)                                                                        cTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCgg                                                                                                                                                                          >  1:147367/1‑251 (MQ=255)                                                                          ttttt‑ATC‑CAGCTGGGTGAGGAATTTCTGTCCTCAACGGTA‑TTTTCCGGGCGAAAAGTTTGCCGGCTCGCGGTTCCAGT‑ATAAACCAAAGCCCAGCATTTGGAGTTGTTTTTTCATA‑TCCGCGATGTTGCCGT‑CCTCCCCGGGTGCCGGGGCGTTTTTTTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCggg                                                                                                                                                                         <  2:16944/250‑1 (MQ=255)                                                                                     aGCTCGGTGAAGAATTTCTTTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCaaa                                                                                                                                                                                                                                  <  1:20110/185‑1 (MQ=255)                                                                                     aGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCaaa                                                                                                                                                                                                                                  >  2:20110/1‑185 (MQ=255)                                                                                             gaagaaTTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGGCTTCCGCAGGAAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGACA‑ACGCTGGTAGCGGCGGATCACGTCACCGaggg                                                                                                                                                       >  2:88015/1‑248 (MQ=255)                                                                                                  aTTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGCTGgtgt                                                                                                                                                    >  1:53443/1‑249 (MQ=255)                                                                                                       ttttCCCAACGGTGTTTTTCCGG‑GGTACGGGTGTCCAGCTCGCGCCCCCAGTAATAA‑CAAAACCCCGCCTTTTTGGGCGGGTTTTTTATA‑TCCGGGTTGTGGTCGCCGGTCCACGG‑GGCGGGGGGGGTGTTTTTTTC‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtac                                                                                                                                              <  2:393187/248‑1 (MQ=255)                                                                                                       tGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacg                                                                                                                                             >  1:252513/1‑251 (MQ=255)                                                                                                          tCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtac                                                                                                                                          >  1:161062/1‑251 (MQ=255)                                                                                                          tCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCGCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGCGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTGCCCAG‑CATACGCTGGTCGCGGGCGCTCACGTCACCAGTGGTGTATgtacgtac                                                                                                                                          >  2:272551/1‑251 (MQ=255)                                                                                                              aaCGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAACGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCCAACGCTTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGGGGAGTTACGCACGTgg                                                                                                                                      >  2:13288/1‑251 (MQ=255)                                                                                                               aCGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGc                                                                                                                                     >  1:46596/1‑251 (MQ=255)                                                                                                                 ggTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑GGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCAAGTCACCGATGGTGTAGTTACGTACGTGGccc                                                                                                                                   >  2:336924/1‑251 (MQ=255)                                                                                                                   aagtaTTCCGG‑CGTACAGGTTGCCAGCCCGCGGCTCCAGACATAA‑CAAAAGCCAAGCATTTTGGGCTGGTTTTTCATA‑TCCGCGATGTTGTCGTACGTCCCCGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAt                                                                                                                                 <  1:51225/250‑1 (MQ=255)                                                                                                                    agtaTTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtg                                                                                                                                >  1:157063/1‑251 (MQ=255)                                                                                                                       aTTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATgtgt                                                                                                                             >  1:222150/1‑251 (MQ=255)                                                                                                                                    gggTTGCCGTCTCGCGGCTCCAGTCATAA‑CAAAATCCCAGCATTTTGAGTTGGTTTTTCATA‑TACGCGATGTTGGCGTACGTCCACGG‑TGCCGGGGCGGTTTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGaa                                                                                                                 <  1:58674/250‑1 (MQ=255)                                                                                                                                          ccAGCTCGCGGCTCCAGTCATAA‑CCAGAGCCCAGCATTTTGAGTTGGTTTTTAATA‑TACGCGATGTTGTCGTACGTCCACGG‑GGCCGGGGCGGTTTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAg                                                                                                           <  1:469606/251‑1 (MQ=255)                                                                                                                                           cAGCTGGCGGCTCCAGTCAAAA‑CCAAAGCCCATCATTTTGAGTTGGTTTTTCATA‑TGCGGGATGTTGTCGTACGTCCACGG‑GGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAgg                                                                                                          <  1:255329/251‑1 (MQ=255)                                                                                                                                                            cATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGGGGCCCATGTGTAGTCGCCCAGAAGGATAGGGAAGCATAgcacagc                                                                                           >  2:38260/1‑243 (MQ=255)                                                                                                                                                              aaa‑CCAAAGCCCACCTTTTGGGGTTGTTTTTTCATA‑TACGCGATGTTGTCGTCCGCCCCCGG‑GGCCGGAGCGGTGTTGTTTTT‑CCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTa                                                                                       <  2:157063/250‑1 (MQ=255)                                                                                                                                                                     aaGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAAGGCGTCCCAGAAGAGAGGCTGCAGGAAGGATTTGCCCAG‑GAGAAGCTGGTAGGGGGGGACAACGTCACCGATGGTGTAGTTACGTACGGGGAACATGTGTAGGAGAACAGAAGGAAAGGGAAGCATAGGAAGGGAGTAATAca                                                                                  >  2:186092/1‑249 (MQ=255)                                                                                                                                                                     aaGCCCAGCATTTTGAGATGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGAGCTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCCTCCCAGCCGATAGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCAAGTCACCGATGGTATAGTTACGTACGTGCACCATGGGTAGTCGACAAGAAGGATAGGGAAGCATAGACAGCCAGTAATACtt                                                                                 >  1:206940/1‑251 (MQ=255)                                                                                                                                                                                               cATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCATGGGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAATTCTCTTTGCTCTCGTCTTCGGGTACtt                                                       >  2:3779/1‑251 (MQ=255)                                                                                                                                                                                                  a‑taCGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGACCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTCCGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCCGTAATACTTCTCTTTGCTCTCGTCTTCGGGTACTTCa                                                     >  1:299100/1‑250 (MQ=255)                                                                                                                                                                                                                 cGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGGAGTTACGTACGTGGCCCATGGGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGAGAGCTtctc                                       >  2:253474/1‑250 (MQ=255)                                                                                                                                                                                                                         cACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATg                              >  1:467487/1‑251 (MQ=255)                                                                                                                                                                                                                               gccggggggtgttttgttTTC‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATTCTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCGTCCCAATGAAGCt                         <  2:164097/238‑1 (MQ=255)                                                                                                                                                                                                                                gCCGGAGCGGTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAATTCTCTTTGCTCTCGTATTCGGTTACTTCCAATGTGAGCTTCTCATCCCAATGAAGCTg                        >  2:36196/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggTGTTGTTTTT‑CACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGAGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTCCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTgg                >  2:169379/1‑251 (MQ=255)                                                                                                                                                                                                                                               gtttttacccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGCTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCt           <  1:270759/242‑1 (MQ=255)                                                                                                                                                                                                                                                      aCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTc   >  2:256877/1‑251 (MQ=255)                                                                                                                                                                                                                                                       ccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTcc  >  2:334987/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       TTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTT‑ATA‑CAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGG‑CGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAA‑CCAAAGCCCAGCATTTTGAGCTGGTTTTTCATA‑TACGCGATGTTGTCGTACGTCCACGG‑TGCCGGAGCGGTGTTGTTTTT‑CACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAG‑CATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCC  >  NC_000913/674274‑674763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑