Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,976	C→T	intergenic (+1419/‑225)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,976	0	C	T	100.0%	253.6 / NA	72	intergenic (+1419/‑225)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/34);  total (38/34)

CGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTC  >  NC_000913/3951729‑3952218                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc                                                                                                                                                                                                                                                 >  1:54549/1‑251 (MQ=255)   gAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCGCGCGACCAAAAGATACCGTCGTCTGTATTTCCCGGGACGGCTCTTTCATGTTGAATGTGCAAGAGCTGGGCACCGTAAAAAGCAAGCAGTTACCGTTGAAAAACGTCTTACTCGATAAACAACGGTTTGGGATGGGTTGTCAATGGCCGCAACTTTTTTTCCAGGAACGATATAGcc                                                                                                                                                                                                                                                >  2:126472/1‑249 (MQ=255)      aaTTTCAGCACCTCCAGCGGCTTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCTACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAac                                                                                                                                                                                                                                            <  1:234853/251‑1 (MQ=255)              caCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTa                                                                                                                                                                                                                                    <  1:248868/251‑1 (MQ=255)                ccTCCAGCGGCTTAGGCACCATGGGTTTTGGTTGAACGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCATCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTAcc                                                                                                                                                                                                                                  <  1:349136/251‑2 (MQ=255)                      gCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTGGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGAGGGTTCGCCAATGGCAGCAACTGTTTTGCCAGGAACGATATAGCGAAACCACCCTTACCGATAAc                                                                                                                                                                                                                            <  2:172151/251‑1 (MQ=255)                         gcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAcccc                                                                                                                                                                                                                         <  2:176540/249‑1 (MQ=255)                                  ccATGGTTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGAGGAATGTGCAAGAGCTGGGCACCGTAAACCGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTc                                                                                                                                                                                                                <  2:277833/251‑1 (MQ=255)                                    aTGGGTTTTGGTTTCCCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAt                                                                                                                                                                                                              <  2:79566/251‑1 (MQ=255)                                    aTGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATCCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTGCCTCAt                                                                                                                                                                                                              <  2:189470/251‑1 (MQ=255)                                      gggTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACCCAAGCAGTTACCGGTGAAAATCGTCTTAATCGATAACCAACGGGTAGGGCTGGTTCGACAATGGCAGCAAATGTTTTTCCAGGAACGAAATAACGGAACCACCCTTACCGCTAAACCCGATTTCCTCaagt                                                                                                                                                                                                            >  2:100892/1‑248 (MQ=255)                                        gTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGtt                                                                                                                                                                                                           >  1:376604/1‑250 (MQ=255)                                               tttACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCCAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGCTATAGCGAAAACAACCTTACNCATAACCCCGATTTCCTCCTGtttaccaccc                                                                                                                                                                                                   >  2:226047/1‑243 (MQ=255)                                                           ggTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATcc                                                                                                                                                                                       <  1:40625/251‑1 (MQ=255)                                                               ggCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTgg                                                                                                                                                                                   <  1:31058/251‑1 (MQ=255)                                                               ggCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTgg                                                                                                                                                                                   >  1:150998/1‑251 (MQ=255)                                                                  gcgcaAGTCGAGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCCGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGcc                                                                                                                                                                                 >  2:197122/4‑250 (MQ=255)                                                                        gTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACAt                                                                                                                                                                          <  2:115677/251‑1 (MQ=255)                                                                             gcgacaaaaCGATACCGTCGTCTGTATCTCCGGTAACGGCTCTTTCATGAAGAATGTGCAAGAGCTGGGCACCGTAACACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCCGCAACTGTTTTTCCAGGAACGCTATAGCGAAACCACCCTTACCTATAACCCCGATTTCCTCATGTTCGCCAGCGCCTTCGGCATCCCTGGCCCACACATCcccc                                                                                                                                                                     >  2:373882/8‑251 (MQ=255)                                                                                 ccaaaCGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACACTGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCgta                                                                                                                                                                  >  1:207574/4‑248 (MQ=255)                                                                                  caaaCGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCCGCGCCTTCGGCATCCCTGGCCAACACATCACCCgtaa                                                                                                                                                                 >  2:358652/3‑247 (MQ=255)                                                                                    aaCGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCgtaa                                                                                                                                                                 >  2:325845/1‑245 (MQ=255)                                                                                          aCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGt                                                                                                                                                        >  1:137642/1‑251 (MQ=255)                                                                                           ccGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCCCCCGTAAAGCCCAgggt                                                                                                                                                       >  2:69005/1‑249 (MQ=255)                                                                                            cgtcgTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCACTGGCCAACACATAACCCGTAAAGACCAggctg                                                                                                                                                      >  2:33738/5‑248 (MQ=255)                                                                                                          ccGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATTCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGac                                                                                                                                        <  1:161344/251‑1 (MQ=255)                                                                                                            ggggACGTCTCTTTCATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGACGGTTCGACAATGGCTGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCTCTCGacac                                                                                                                                      <  2:96026/248‑1 (MQ=255)                                                                                                                     tctTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGa                                                                                                                              >  1:476428/1‑250 (MQ=255)                                                                                                                           atgatgAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGGCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGa                                                                                                                       <  1:408201/251‑1 (MQ=255)                                                                                                                              atgaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGGTGAAGCGGCACTCGACACCATGCTGAACAGGGATgg                                                                                                                    >  2:350823/1‑251 (MQ=255)                                                                                                                               tgaATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGCTTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGAGACACTCGACACCATGCTGAACAGTGATggg                                                                                                                   >  2:17005/1‑251 (MQ=255)                                                                                                                                  aTGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTTGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGcc                                                                                                                 >  1:481076/1‑250 (MQ=255)                                                                                                                                  aTGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGcc                                                                                                                 >  1:352268/1‑250 (MQ=255)                                                                                                                                      gCAAGAGCTGGTCACCGTAAAACGCAAGCAGTTACCGTTGAAAAGCGGCTTACACGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCTCCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATAcc                                                                                                            <  2:81010/251‑1 (MQ=255)                                                                                                                                        aaGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCATCCTTACCGCTAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTg                                                                                                          >  1:394210/1‑251 (MQ=255)                                                                                                                                           agCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCtt                                                                                                       <  1:325845/251‑1 (MQ=255)                                                                                                                                            gCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTc                                                                                                      <  1:149849/251‑1 (MQ=255)                                                                                                                                               gggCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCa                                                                                                     <  1:20118/249‑1 (MQ=255)                                                                                                                                               gggCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCAt                                                                                                    <  1:438103/250‑1 (MQ=255)                                                                                                                                               gggCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTGTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACACGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATg                                                                                                   <  1:34557/251‑1 (MQ=255)                                                                                                                                                      gTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAAt                                                                                            <  1:126472/251‑1 (MQ=255)                                                                                                                                                                 gCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACAt                                                                                                                                                                          >  2:72757/1‑162 (MQ=255)                                                                                                                                                                    gTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGCACGATATAGCGAAACCACCCCTACCGATAACCCCGATTTCCTCATGTTAGCCACCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGATGAACAGTGATGGGCCATAACTGCTTCATGTCTCAATCGACGAACTTg                                                                                 >  2:125312/1‑248 (MQ=255)                                                                                                                                                                       aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCAt                                                                                                               >  1:67534/1‑215 (MQ=255)                                                                                                                                                                       aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCAt                                                                                                               <  2:67534/215‑1 (MQ=255)                                                                                                                                                                       aCCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACg                                                                           >  1:151987/1‑251 (MQ=255)                                                                                                                                                                          gTTGAAACTCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATTTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACCGTGATGGGCCATACCTGCTTCCTGTCTCAATCGCCGACCTTGAGAACGTcc                                                                        >  2:167854/1‑250 (MQ=255)                                                                                                                                                                             gAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGc                                                                     >  1:375310/1‑251 (MQ=255)                                                                                                                                                                              aaaaTCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGcc                                                                    <  1:52726/251‑1 (MQ=255)                                                                                                                                                                                    gTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGt                                                              >  1:77614/1‑251 (MQ=255)                                                                                                                                                                                       ttACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTgcc                                                           >  1:195566/1‑251 (MQ=255)                                                                                                                                                                                             gATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGa                                                                                                                              <  2:341265/178‑1 (MQ=255)                                                                                                                                                                                             gATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGa                                                                                                                              >  1:341265/1‑178 (MQ=255)                                                                                                                                                                                                    aaCGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGt                                              <  1:226047/251‑1 (MQ=255)                                                                                                                                                                                                      cGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTaa                                            <  1:34521/251‑1 (MQ=255)                                                                                                                                                                                                                 ggTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCCCTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGGCTGGCCGCTGGTGCCGCCTGGTGCCCGTAATTCCGAAATGt                                 >  2:120174/1‑251 (MQ=255)                                                                                                                                                                                                                 ggTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAGGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATg                                  <  2:375310/250‑1 (MQ=255)                                                                                                                                                                                                                  gTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACACTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGCTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATAACTGCTTCCTGTCTCCATCAACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGtt                                >  2:466322/1‑251 (MQ=255)                                                                                                                                                                                                                      gACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCTGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGgag                            <  1:458653/251‑1 (MQ=255)                                                                                                                                                                                                                      gACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGgag                            <  2:315695/251‑1 (MQ=255)                                                                                                                                                                                                                       aCAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTgg                              >  1:432901/1‑248 (MQ=255)                                                                                                                                                                                                                         aaTGGCAGTAACTGTTTTTCCAGGAACGATATAAAGAAACCACACTTCCCTATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCCACACATCACCCGTAAACACCTGGTTGGAGCGGCCCTCGACAACATGCTGCACAGTGATGGGCCATACCTGCTTCAGGTCTCAATCGAAGAACATGAGAACATCTGCCCGCTGGAGCCGACAGGAGCCAGTAATTAAGCAATGTTGgaaac                          >  2:364643/1‑247 (MQ=255)                                                                                                                                                                                                                         aaTGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCGCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGaaa                         <  1:167854/251‑1 (MQ=255)                                                                                                                                                                                                                             gcagcaACTGTTTTGCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTAt                     <  2:54549/251‑1 (MQ=255)                                                                                                                                                                                                                                gcaACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCat                  <  2:376604/251‑1 (MQ=255)                                                                                                                                                                                                                                 caACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatg                 <  1:100892/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaCTGTTTTTCCAGGACCGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCAAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGGCACCATGCTGAACAGTGATGGGACATACCTGCTTCATGTCTCAATCGACGAACTTGATAACGTCGTGCAGCTGGTGCCGCCTGGTGCACNAAATTAAGAAAGGTTGGAGAAATTCTCa                   >  2:240403/1‑248 (MQ=255)                                                                                                                                                                                                                                   aCTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCatgat               >  1:246124/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttccAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAt        >  1:379659/4‑251 (MQ=255)                                                                                                                                                                                                                                            ccAGGAACGATATAGCGAAACCTCCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCAGCCCTGGCCAACACATCTCCCGTAATGACCAGGTTGAAGCGGCACTCGACTCCATGCTGAACAGTGATGTGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTTGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATc       <  1:225744/249‑1 (MQ=255)                                                                                                                                                                                                                                                 aaCGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGCACTCGACACAATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTc  >  1:119493/1‑250 (MQ=255)                                                                                                                                                                                                                                                 aaCGATATAGCGAAACCACCCTTACAGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCACTGGCCAACACATCACCCGTAAAGACCAGGCTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGAGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTc  >  1:169146/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACATCAGGTC  >  NC_000913/3951729‑3952218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑