| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,951,796 | A→G | intergenic (+1239/‑405) | ilvX → / → ilvM | uncharacterized protein IlvX/acetolactate synthase II subunit IlvM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,951,796 | 0 | A | G | 97.0% | 173.5 / ‑3.3 | 66 | intergenic (+1239/‑405) | ilvX/ilvM | uncharacterized protein IlvX/acetolactate synthase II subunit IlvM |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (24/40); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
ACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGG > NC_000913/3951553‑3952041 | acacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTTCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCTCATCGCCCACACTCGCCCGGAAAATTTCATCTCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTc < 1:373605/250‑1 (MQ=255)acacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGGGCGCAAGTcg > 1:62104/1‑251 (MQ=255) gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGAt > 1:191923/1‑250 (MQ=255) cGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATAc < 1:213212/251‑1 (MQ=255) gTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCCGTTGGCGCGCAAGTCGCGCGACCAAACGATAcc > 1:315695/1‑251 (MQ=255) gatgaACATGCCTGTCGGTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCTGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGt < 2:14752/251‑1 (MQ=255) aCATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCt < 1:296083/251‑1 (MQ=255) aCATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATAACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCCAAccgcaccgtcgtct > 2:20118/1‑238 (MQ=255) cctgccTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTAGTTGTTAAAACAACTGTCAGTACGTAAACCTTCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATTTGGTCTGCGCAACACATCGCCCCCACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGTGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑g < 2:177726/247‑1 (MQ=255) ggCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGGTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTcc < 2:288172/251‑1 (MQ=255) gACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACgg < 1:28529/251‑1 (MQ=255) cccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGc < 2:149572/250‑1 (MQ=255) aCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCGGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGc < 2:69403/251‑1 (MQ=255) gTGACGCTATCTACGCTCCGTTGTTGTTAAAACCACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCCCATCTCCCCCACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTGGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCatg < 1:470692/251‑1 (MQ=255) cTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCT‑GTATCTCCCGTGACGGCTCTTTCctgatgca > 2:63307/1‑248 (MQ=255) gcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATAACGGCGGCT‑GTATCTACGGGGACGGCTCTTTCATGATGAATGAGGAagagg > 2:52726/1‑250 (MQ=255) tgttAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCAc < 1:99127/250‑1 (MQ=255) ggtAAAACTACTGTCAGATTGTAACCCTGCGGATTGCGTCGTGACGACATATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACCCTGGCCCGGAGAATTTCATCCCCGCCAGCGGCTTAGTCGCCATGGGGGTTGGGTGACCGGCGTTGGGTGGCGCGCAAGGCGCGCGACCAAACTATATCGGTGTCG‑GTATCTCCGGTGACGGCTCTTTCGTGATGAATGTGCAAGAGCTGGGCAcc < 1:188275/248‑1 (MQ=255) aaaCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGGGCACAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGGACCGTaa > 1:202389/1‑250 (MQ=255) aacaacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTcc < 2:451648/203‑1 (MQ=255) aacaacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTCGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGGAAGTCGCGCGACCAAACGACACCGGCGTCC‑GTATCTcc > 1:451648/1‑203 (MQ=255) acTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGAGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCTGCGGTTGGCGCGCAAGGAGCGCGACCAAACCATACCGTCGTCT‑GTCTCTCCGGTCACGGCCCTTTCCTGATGAATGGTCTAGAGCTggggcccggataacgcc > 2:86215/1‑237 (MQ=255) gtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCGCCAGACGCGCGACCCAACCACACCCACGTCTTGT‑TATCCGGCGCCGGCTCTTTCATGATGACTGTGCAAGTGCTGGGccacgcacaaagtccgc > 2:94338/5‑235 (MQ=255) gtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGCGCACCGTAAAACG‑CAAGc < 2:409858/247‑1 (MQ=255) cGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCCAACGATACCGGCGGCT‑GTATCTCCGGGGACGGCTCTTTCAATATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑ccaacaggtacc > 2:467456/1‑240 (MQ=255) ccTGGGGATTGCTGCGTGACCCCAGAGGTGGGGCAGCGCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCTGCTTAGGCACCATTGGTTGTGGTTTACCGGCGGCGGTTGGCGCGCAATTCGCGCGACCAAACGATACCGTCGTCT‑GTAGCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCGGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGat < 2:78858/251‑2 (MQ=255) ggATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGCGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAAGATACCGTCGTCT‑GTTTCCCCGGTGACAGCCCTTTCATGATGAATGTGCAACAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCCTTGTAAAt > 1:175486/1‑249 (MQ=255) tGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCt < 1:114142/250‑1 (MQ=255) gCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTAACCGTTGAAAATCGTCtt < 2:401999/250‑1 (MQ=255) gtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACt > 1:195108/1‑251 (MQ=255) gtcgtGAACACCGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCCCAGCGCCCACTCTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTGTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCGTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAAGCGTCTTACt < 2:266724/251‑1 (MQ=255) ccACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAc < 1:252089/251‑1 (MQ=255) aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc < 1:381738/251‑1 (MQ=255) aggtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCGCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACgg < 2:202389/249‑1 (MQ=255) tgtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCACGCCAATCGCGCCACCAAAAGATACCGTCGTCT‑GTATCTCCGGTCACCGCTCTTTCATGATGAATGGGCAAGAGCTGGGCAACGTAAAACGACAA‑CAGTTATCATTGAAAATCGACTTACTCCATACCCAACggg > 2:346111/1‑250 (MQ=255) ggggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAg < 1:63307/251‑1 (MQ=255) gggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAgg < 1:148946/251‑1 (MQ=255) ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACCGTTatgg > 2:149849/1‑248 (MQ=255) cagcaCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGAt < 2:335708/251‑1 (MQ=255) aCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGtt < 1:238240/251‑1 (MQ=255) aCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAAGATAACGTCGTCT‑GTATCTCCGGGGACGGCTCTTTCATGATTAATGTGCAAGAGCTGGGCCCCGTAAAACG‑CAAGCAGTTACCGGTGAAAATCGTCCTACTCGATAAACAACGGGTAGGGAttggt > 2:34521/1‑247 (MQ=255) gATGTGGGCTGCGCAGCACATCGCCCCCACTNGCCCGGAAAATTTCATCACCTCCAGCGGATTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTCCTCGATAGCCAACGGTTAGGGATGGTTCGAc < 2:227896/251‑1 (MQ=255) gtgtgGGCTGCGCAGCACAGCGCCCACACGCNCCCGTAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGATTTTGGTGTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGGCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCTAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTAGCGTTGAAAATCGTCTTACTCCATAACCAACGGTTATGGATGGTTCGACa < 2:234096/250‑1 (MQ=255) agctcaTCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTg < 1:241764/247‑1 (MQ=255) caTCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAATCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCCGTTAACGTTGAAAATCGTCTTACTCGAAAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGtttt > 1:96026/1‑251 (MQ=255) aTCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGAACAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTGCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGttttt < 2:459278/251‑1 (MQ=255) cGCCCACACTCGCCCGGAAAATTTCATCACNCCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGtttttc < 2:224874/250‑2 (MQ=255) cGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGa < 2:195108/249‑1 (MQ=255) ccGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCACGTCGCGCGAGCCAACGCTAACGTCGTCT‑GTATCTCCGGGGAAGGCTCCTTAATGATGAATGTGCAAGAGCCGGGCACCGTAAAACG‑CAAGCAGCTACCGTTGAAAATCGTCTTACTCGATAACCAACGGGTAGGGGTGGGTCGACAANGGCAGAAACTGGTTTTTCAGGaaaggtct > 2:234853/1‑243 (MQ=255) cGGAACATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGCTACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTTATGTTGAATGTGCAAGAGCTTGGCACCGTAACACG‑CCAGCCGTTACCGTTGAAAATCGTATTAATCGATCAACAACGGTTCGGGATGGTTTGACCAANGCCGCAACTGTTTTTTCCAGAACGATaata > 2:222677/1‑247 (MQ=255) cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGc > 1:54549/1‑251 (MQ=255) gAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCGCGCGACCAAAAGATACCGTCGTCT‑GTATTTCCCGGGACGGCTCTTTCATGTTGAATGTGCAAGAGCTGGGCACCGTAAAAAG‑CAAGCAGTTACCGTTGAAAAACGTCTTACTCGATAAACAACGGTTTGGGATGGGTTGTCAATGGCCGCAACTTTTTTTCCAGGAACGATATAGcc > 2:126472/1‑249 (MQ=255) aaTTTCAGCACCTCCAGCGGCTTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCTACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAac < 1:234853/251‑1 (MQ=255) caCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTa < 1:248868/251‑1 (MQ=255) ccTCCAGCGGCTTAGGCACCATGGGTTTTGGTTGAACGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCATCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTAcc < 1:349136/251‑2 (MQ=255) gCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTGGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGAGGGTTCGCCAATGGCAGCAACTGTTTTGCCAGGAACGATATAGCGAAACCACCCTTACCGATAAc < 2:172151/251‑1 (MQ=255) gcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAcccc < 2:176540/249‑1 (MQ=255) ccATGGTTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGAGGAATGTGCAAGAGCTGGGCACCGTAAACCG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTc < 2:277833/251‑1 (MQ=255) aTGGGTTTTGGTTTCCCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAt < 2:79566/251‑1 (MQ=255) aTGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATCCCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTGCCTCAt < 2:189470/251‑1 (MQ=255) gggTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCCCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACC‑CAAGCAGTTACCGGTGAAAATCGTCTTAATCGATAACCAACGGGTAGGGCTGGTTCGACAATGGCAGCAAATGTTTTTCCAGGAACGAAATAACGGAACCACCCTTACCGCTAAACCCGATTTCCTCaagt > 2:100892/1‑248 (MQ=255) gTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGtt > 1:376604/1‑250 (MQ=255) tttACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CCAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGCTATAGCGAAAACAACCTTACNCATAACCCCGATTTCCTCCTGtttaccaccc > 2:226047/1‑243 (MQ=255) ggTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATcc < 1:40625/251‑1 (MQ=255) ggCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTgg < 1:31058/251‑1 (MQ=255) ggCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTgg > 1:150998/1‑251 (MQ=255) | ACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCT‑GTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACG‑CAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGG > NC_000913/3951553‑3952041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |