Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,549	T→C	intergenic (+63/‑37)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,549	0	T	C	100.0%	173.2 / NA	51	intergenic (+63/‑37)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aaaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAt                                                                                                                                                                                                                                                <  1:84771/250‑1 (MQ=255)  aaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                                                                              >  2:119164/1‑251 (MQ=255)       ggTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaa                                                                                                                                                                                                                                         <  1:364423/251‑1 (MQ=255)            ccTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCg                                                                                                                                                                                                                                    <  1:194250/251‑1 (MQ=255)            ccTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCg                                                                                                                                                                                                                                    >  1:296894/1‑251 (MQ=255)                            aaTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGt                                                                                                                                                                                                                     <  2:75160/250‑1 (MQ=255)                             aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTGAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTa                                                                                                                                                                                                                   <  1:177294/251‑1 (MQ=255)                               ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                                 >  1:40789/1‑251 (MQ=255)                               ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                                 <  2:315070/251‑1 (MQ=255)                                         gcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCGTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTTTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTg                                                                                                                                                                                                       <  2:285605/251‑1 (MQ=255)                                            aaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                                                                     >  1:9416/1‑250 (MQ=255)                                             accgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAt                                                                                                                                                                                                   <  1:119164/251‑1 (MQ=255)                                                  gaactgtagacattaattgTGATTATCTCATNAATGTAATCGAAAATTCTTACAACAACGGGTGTGTTGAGTTTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTTCGTTGGGTGGTTGCTTTCCGTGAAATCTGTA‑CGCAATTCAGAGAAGGCTTTTTTAGGCCTATAAGTGGTCATGGGATCTTGTTGTCCGAATTCAGGAAAGGCGAGATTTACAAATGGTTAATTATGAAACTGGGaggtatt                                                                                                                                                                                              <  2:216494/240‑7 (MQ=255)                                                   aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGCAAATTCTGACTACAACGGTTAGGTTGGGTGTGAATATAAACCCCAAACCACCACGGCAGCCGGTTTACGCTGGATGGATCCGTAACGTTAAAAC‑GTGACGCTATTCTGACAATGCTTTTTTAGGCCGCTAAGTAGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTCTACAACTGGGATTTATTg                                                                                                                                                                                             >  2:439327/1‑251 (MQ=255)                                                                    aTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagt                                                                                                                                                                            >  1:245724‑M1/1‑246 (MQ=255)                                                                           tctcTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggc                                                                                                                                                                     >  1:483866‑M1/1‑239 (MQ=255)                                                                                  aaGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGGTGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                                                                              <  1:428071‑M1/251‑20 (MQ=255)                                                                                   aGGTAATCGAAAATTCTGACTACAAAGGTAGGATTGGGTGTGAATATCAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACAGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGACCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGTATTAgtagtagtaggcattagtc                                                                                                                                                              >  1:411835‑M1/1‑231 (MQ=255)                                                                                       aaTCGAAAAATCTGACTAAAACGTTTGGGTTGGGTGTGAATATAAACCCCAAACCTCCACGGAAGACGTTTTACGCTGGATGGATTCGTCCCGGTAAAAC‑GTGACGCTATTCAGACTATGCTTTTTTAAGCCGCTTAGTTGGCAGGGGATCGTGTTGTCCGAATACAGTAAAATCGAAAGTTAAAAGAGGTTAATTATGAAACTGAGATTTATTGTCTTAGGCATTagtagtcgtaggcattagacatttg                                                                                                                                                         <  1:208130/251‑25 (MQ=255)                                                                                                  cTGACTACAACGGATGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATAGTGTTGTCCGAATTCAGGAATAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTCGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcg                                                                                                                                              >  2:160320‑M1/1‑216 (MQ=255)                                                                                                        aCAACGGTTGGGTTGGGTGTGAATATAACCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgctt                                                                                                                                        <  1:98967‑M1/251‑42 (MQ=255)                                                                                                                 gggttgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtct                                                                                                                               <  1:76773‑M1/251‑51 (MQ=255)                                                                                                                     tgggtGTGAATATAAACCCCAATCCACCACTGTAGCCGGGTTACGCTGGATGGATCCGTACCGTTAAAAC‑GGTACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATGGATTGGCTTATGCATTAgtagtcgtagacattagacatttgttccttatgcgacttgtttcgcgtcttat                                                                                                                            <  2:316517‑M1/250‑54 (MQ=255)                                                                                                                     tgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttat                                                                                                                            <  2:9416‑M1/250‑54 (MQ=255)                                                                                                                                         aaaCCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgc                                                                                                       >  1:443913‑M1/1‑177 (MQ=255)                                                                                                                                                    gAAGCCTGTTTACGATGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATGCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttataaggcgtacaaccggtgccgcatccggcaatt                                                                                            <  1:264574‑M1/251‑86 (MQ=255)                                                                                                                                                     aaGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcctccggcaattg                                                                                           >  1:293850‑M1/1‑165 (MQ=255)                                                                                                                                                         cGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattag                                                                                                                                                                >  2:86457‑M1/1‑161 (MQ=255)                                                                                                                                                         cGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattag                                                                                                                                                                <  1:86457‑M1/178‑18 (MQ=255)                                                                                                                                                           gTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcac                                                                                     >  2:159800‑M1/1‑159 (MQ=255)                                                                                                                                                             ttACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcaca                                                                                    <  2:100975‑M1/250‑94 (MQ=255)                                                                                                                                                                           tCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGGAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggagcacaatgcctgatgcgatg                                                                     >  1:311083‑M1/1‑143 (MQ=255)                                                                                                                                                                             cGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgct                                                                   <  2:296894‑M1/251‑111 (MQ=255)                                                                                                                                                                              gTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                                  >  2:141869‑M1/1‑140 (MQ=255)                                                                                                                                                                              gTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                                  >  2:347236‑M1/1‑140 (MQ=255)                                                                                                                                                                                      aaaaC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcat                                                           <  1:455689‑M1/250‑119 (MQ=255)                                                                                                                                                                                         aC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttt                                                       >  1:266241‑M1/1‑129 (MQ=255)                                                                                                                                                                                               aCGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTa                                                                                                                                                                                        >  1:71534/1‑117 (MQ=255)                                                                                                                                                                                               aCGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTa                                                                                                                                                                                        <  2:71534/117‑1 (MQ=255)                                                                                                                                                                                                       cAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaat                                          >  1:12800‑M1/1‑116 (MQ=255)                                                                                                                                                                                                        aGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGAGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTGAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctttacgcatcttatcaggcctacactg                                         >  2:384711‑M1/1‑115 (MQ=255)                                                                                                                                                                                                          aCAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatggg                                       >  1:223726‑M1/1‑113 (MQ=255)                                                                                                                                                                                                              tGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGATGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                   >  1:153047‑M1/1‑109 (MQ=255)                                                                                                                                                                                                                    tttAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAGTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatc                              <  1:262028‑M1/250‑148 (MQ=255)                                                                                                                                                                                                                       aGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggt                           <  1:293825‑M1/250‑151 (MQ=255)                                                                                                                                                                                                                        ggCCGCTAAATTGGCAGGGGATCTTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtag                         <  2:128596‑M1/251‑153 (MQ=255)                                                                                                                                                                                                                             cTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgctgcgatgcttgacgcatcttatcaggcctacaatgggtcccggatcggtaggccgg                    >  2:315510‑M1/1‑94 (MQ=255)                                                                                                                                                                                                                             cTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcctcttatcaggcctacaattgttaccggatcggtaggccgg                    >  2:315501‑M1/1‑94 (MQ=255)                                                                                                                                                                                                                               aaGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgcaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggat                  >  2:44142‑M1/1‑92 (MQ=255)                                                                                                                                                                                                                                        ggggATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggaaaaggagttt         >  2:290754‑M1/1‑83 (MQ=255)                                                                                                                                                                                                                                               gtgtTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGGTAATTATGAACCTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagtcatttgtgcctgatgctacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgc  <  2:138751‑M1/251‑176 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   AAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGT  >  NC_000913/536304‑536791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑