Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,091	A→G	N153N (AAT→AAC)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,091	0	A	G	98.7%	259.8 / NA	75	N153N (AAT→AAC)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (49/25);  total (50/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACG  >  NC_000913/3815846‑3816324                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          cgccgcCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCACGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTgg                                                                                                                                                                                                                                       >  1:102560/1‑251 (MQ=255)  gccgccTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGt                                                                                                                                                                                                                                      >  1:451433/1‑251 (MQ=255)  gccgccTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGt                                                                                                                                                                                                                                      <  1:3097/251‑1 (MQ=255)     gccTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGtttt                                                                                                                                                                                                                                   >  1:58825/1‑251 (MQ=255)         tcggCGTCGCTACAATGGATTCGATTCCCCCGCGGGCCAGCGTCAACAAGGTGAGTAGTTCGTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAg                                                                                                                                                                                                                                <  2:333823/247‑1 (MQ=255)          cTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCACTTTCATCGGGTTGGTTTTAAGCt                                                                                                                                                                                                                              >  2:484868/1‑251 (MQ=255)            gCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATAGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTg                                                                                                                                                                                                                            >  2:54780/1‑251 (MQ=255)             cGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGGCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCCACGTATTCCAGATCGCAAATTGCTTCACCGTGTACAATACCGACAGAAACTGCGGCTACCATCCCCTTCATCGGATTGGTGTTAAGCTTGc                                                                                                                                                                                                                           >  2:441607/1‑251 (MQ=255)              gTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTAAGCtttcc                                                                                                                                                                                                                          >  2:336014/1‑248 (MQ=255)                                           ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGCGTCTTCAACGTATTCCAGATCGCCAATTGCTTCACCGTTTACAATACCGGCCGAAACTGCGGCTACCATCCCTTTCCTCGGGTTGGTTTTTAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtg                                                                                                                                                                                             >  2:3875/1‑250 (MQ=255)                                                 cgCCAACAAGGTGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGCCAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAgcg                                                                                                                                                                                       <  2:384951/250‑1 (MQ=255)                                                   ccAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGcc                                                                                                                                                                                     >  2:343680/1‑251 (MQ=255)                                                     aacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCAc                                                                                                                                                                                   >  1:359826/1‑251 (MQ=255)                                                                     ctTCATGGGTGCACGGCTCGCCTTCTGCCGTCCCCTGCAATTCATTGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGACTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTAACCGTTTACAATACCGACAGAAACTGCGGCTACCATCACTTTCATCGGGTTGGTTTTCAGCTTGCCGTGTTCCACCCGATTCTGGAGCGNATCTTACAGCGCCACGCACGCCCCCGAAATc                                                                                                                                                                   >  2:233229/1‑251 (MQ=255)                                                                       tCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGAGGCGCCCGGCTTCGGGCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGTCAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCATGCACCCGTAAt                                                                                                                                                                    <  2:463088/248‑1 (MQ=255)                                                                        cATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCCCCCGTAATCGAc                                                                                                                                                                >  1:120078/1‑251 (MQ=255)                                                                                   ggCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGt                                                                                                                                                     >  1:135351/1‑251 (MQ=255)                                                                                        gCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGccac                                                                                                                                                >  1:340805/1‑251 (MQ=255)                                                                                               gCCGTGCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGCCGCGGTACGCGTGCCACCATCAGc                                                                                                                                         >  1:318820/1‑251 (MQ=255)                                                                                                  gTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCACCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTg                                                                                                                                      >  2:53385/1‑251 (MQ=255)                                                                                                         gctcTTCAATGATGCGCCCGGCTTGGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTCCGCGTGCCACCATCAGCCTGAAGCACg                                                                                                                               <  2:451433/248‑1 (MQ=255)                                                                                                          cACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGt                                                                                                                              >  1:335636/1‑251 (MQ=255)                                                                                                           aCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTc                                                                                                                             >  2:263490/1‑251 (MQ=255)                                                                                                                   gatgCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGGACGCGTGCCACCATCAGCCTGACGCACGTCGCAGTccc                                                                                                                     >  2:258966/1‑250 (MQ=255)                                                                                                                       cgcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGt                                                                                                                 <  1:144854/251‑1 (MQ=255)                                                                                                                           cGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATg                                                                                                             <  1:12517/251‑1 (MQ=255)                                                                                                                            gTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATgg                                                                                                            <  1:204313/251‑1 (MQ=255)                                                                                                                              cTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTg                                                                                                          >  1:124303/1‑251 (MQ=255)                                                                                                                                tCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGa                                                                                                         <  1:343680/250‑1 (MQ=255)                                                                                                                                  ggTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACt                                                                                                      >  2:15107/1‑251 (MQ=255)                                                                                                                                     catcaCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAc                                                                                                   <  1:198140/251‑1 (MQ=255)                                                                                                                                            aCGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAgcgc                                                                                            >  2:483049/1‑251 (MQ=255)                                                                                                                                                tCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCTACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCttt                                                                                         <  1:72766/250‑1 (MQ=255)                                                                                                                                                tCATGTCGGTCTCTGCGGCTGAGCCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTc                                                                                        >  1:278440/1‑251 (MQ=255)                                                                                                                                                       ggTCTCTGCGGCTGAGACTTCAACGTATTCCAGCTCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCCGCTTGCCGTTTTCCACCAGCTTCTGCAGCGCATCTGCCAGCGCCACGCCGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCCCGTCGCCGTCCAGCGCAATGGCTAACTCACCCAGCGCTTTCAAATCt                                                                                  >  2:32553/1‑250 (MQ=255)                                                                                                                                                            ctGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGAGAACTCACCCAGCGCTTTCAACTCTACTGcc                                                                            >  2:74056/1‑251 (MQ=255)                                                                                                                                                              gCGGCTGATTCTTCAACGGATTCCAGAACGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCATGCACCCGTAATCGACGCGGTACGCGTGCCACCAGCAGCCTGAAGCACGTCGCAGTCCAGCTTAATGGTTAACTCACCCAGCGCTTTCAAATCTACTGc                                                                             <  1:414811/248‑1 (MQ=255)                                                                                                                                                                    gagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGa                                                                      >  2:120205/1‑249 (MQ=255)                                                                                                                                                                    gagTCTTCAACGTATTCCAGAACGCAAATTGCGTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAag                                                                    <  2:481161/251‑1 (MQ=255)                                                                                                                                                                     agTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGa                                                                      >  1:345256/1‑248 (MQ=255)                                                                                                                                                                        cTTCAACGTATTCCCGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCCCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCCCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGcc                                                                >  2:180192/1‑250 (MQ=255)                                                                                                                                                                          tCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGc                                                                 <  1:246107/248‑1 (MQ=255)                                                                                                                                                                          tCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGATGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCNCTTTCAAATCTACTGCCGCGCGAAGAGCACg                                                              >  2:217894/1‑251 (MQ=255)                                                                                                                                                                          gcAACGTATTCCAGATCGCAAAGTGCTGCAACGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCTCGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCAc                                                               <  2:395653/249‑1 (MQ=255)                                                                                                                                                                           cAACGTATTCCAGATCGCAAATTGCTGCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACTAGCTTCTGTAGCGCATCTTCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCTGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACgg                                                             <  1:402939/251‑1 (MQ=255)                                                                                                                                                                               gTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCg                                                          >  1:448941/1‑250 (MQ=255)                                                                                                                                                                                tATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCtt                                                                                                                                                                                                           <  1:300431/104‑1 (MQ=255)                                                                                                                                                                                tATTCCAGATCGCAAATTGCTTCACCGTATACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCtt                                                                                                                                                                                                           >  2:300431/1‑104 (MQ=255)                                                                                                                                                                                  ttCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGGGCACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCgctc                                                       >  2:463234/1‑247 (MQ=255)                                                                                                                                                                                     cAGATCGCAAATAGCTTCCCCGTTTACAAGACCGGCACAAACTGCGGCTTCCATCGCTTTCATAGGGTTGGGTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGTCACGCAGGGACCCGGAATCGGCGCGGGACGCGTGCCACCAGCAGCCTGGAGCATGGCGCAGTGTAGCGTAAGGGTTAACTCGCCCAGCGCTTTCAAATCTGCTTCCGCGCGTAGAGCACGGGCAGTCAGAc                                                   <  2:417956/251‑1 (MQ=255)                                                                                                                                                                                       gATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGc                                                 >  1:20121/1‑251 (MQ=255)                                                                                                                                                                                           gcaaattgCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTgg                                              >  2:362145/8‑250 (MQ=255)                                                                                                                                                                                            aaaagtgctgcaccGTTTACAATACCGACAGAAACTGCGGCTACCAGCCCTTTCACCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGAt                                            <  1:63/239‑1 (MQ=255)                                                                                                                                                                                               attgCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTc                                         <  2:335636/248‑1 (MQ=255)                                                                                                                                                                                               attgCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTc                                         >  2:118316/4‑251 (MQ=255)                                                                                                                                                                                                ttgCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTcc                                        >  1:448125/3‑251 (MQ=255)                                                                                                                                                                                                    ttcaccGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGCTCAGACGCTGGATTTCCATtg                                    >  2:375470/5‑251 (MQ=255)                                                                                                                                                                                                      caccGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATtgtg                                  >  1:16600/3‑251 (MQ=255)                                                                                                                                                                                                       accGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGc                                 <  1:252826/250‑1 (MQ=255)                                                                                                                                                                                                         cGTTTACAATCCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGt                               <  1:74056/251‑1 (MQ=255)                                                                                                                                                                                                          gtttacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGGCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTc                              >  2:148606/5‑251 (MQ=255)                                                                                                                                                                                                           tttacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTcc                             >  1:427521/4‑251 (MQ=255)                                                                                                                                                                                                             tacaataacGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACAAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCATGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGATTTCAAATCTACTGCCGCGCGCAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAc                           >  2:162531/9‑251 (MQ=255)                                                                                                                                                                                                              acaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAcc                          >  1:330244/1‑251 (MQ=255)                                                                                                                                                                                                                      gACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTAc                  <  1:148649/251‑1 (MQ=255)                                                                                                                                                                                                                       aCAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTAcc                 >  1:45632/1‑251 (MQ=255)                                                                                                                                                                                                                         aGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCCAATCTCCTGCCGCACGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCtt               >  2:112804/1‑251 (MQ=255)                                                                                                                                                                                                                         aGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCtt               >  1:408025/1‑251 (MQ=255)                                                                                                                                                                                                                         aGAAACTGCGGCTAACATCACTTTCAGCGGGATGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCAGTAGCGCATCTACTAGCGCCCCGCAGGCACCCGGACTCGACCCGGGACGCGTGCCACCATCAGCCTGCAGCACGTCGCAGTCCAGCGTGATGGTGAACTCACCAAGCGCTTTCAAAGCTCATGCCTCGCGTAGAGCACAGGCCCTCAGACGCTGGATTTCCATTGTGCGCCGACACCGCTTACCtt               >  2:350966/1‑251 (MQ=255)                                                                                                                                                                                                                             aCTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTcgc           <  1:256339/251‑1 (MQ=255)                                                                                                                                                                                                                                gCGGCTACCATCCCTTTCCTCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCCGCTTCTGTAGCGCATCTGCCAGCGCCCCGCCGGCCCCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCCGCGTAATGGTGAACTCACCCCGCGCTTTCACATCTACTGCCGCGCGACGAGCACGGGCGCTCCGACGCTGAATTTCCCTTGGGCGTCCACCCTGCTTCCCTTTcgccgc        >  2:153855/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTc     >  1:274276/1‑251 (MQ=255)                                                                                                                                                                                                                                     tACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCAc   >  1:345824/1‑251 (MQ=255)                                                                                                                                                                                                                                      aCCATCCCTTTCTTCTGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGGCGCAGTCCAGCTTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAATAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCAc   <  1:433933/250‑1 (MQ=255)                                                                                                                                                                                                                                        cATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACg  >  1:438415/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACG  >  NC_000913/3815846‑3816324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑