Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,492	A→G	intergenic (+6/‑94)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,492	0	A	G	97.7%	150.2 / ‑6.5	44	intergenic (+6/‑94)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (23/19);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAG  >  NC_000913/536248‑536738                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aTCATATGCAGCGGATGGAAGGAGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGGAATCGAAAATTCTGACTACAACGGTTGGGTTGGGAGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGAAACCGTACCGTTAAAAC‑GTGACGca                                                                                                                                                                                                                                                   >  1:300718/1‑249 (MQ=255)      atGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTc                                                                                                                                                                                                                                               >  1:370586/1‑249 (MQ=255)         cAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGAc                                                                                                                                                                                                                                           >  1:128596/1‑250 (MQ=255)                                     ccATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGACGAGCCGGGAACCGGAGAAATTAATTCTGATTATCTCTTTAAGGTAATCGAACATTCTGACTACAATGGTTGGGTTGGGTGTGAATATAAACCCAAAACCACCGCGGAAGCCGGTTTACGCTGGACGGATCCGTACCGTTAAACC‑GTGAAGCTATTAAGAAACCGCTTTTTTAGGCCTCTAAGATGGc                                                                                                                                                                                                                >  2:365040/1‑249 (MQ=255)                                       aTGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCTCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAAGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAggg                                                                                                                                                                                                            <  1:474651/251‑1 (MQ=255)                                       aTGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAggg                                                                                                                                                                                                            >  1:315070/1‑251 (MQ=255)                                         gACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAgggg                                                                                                                                                                                                           >  1:75160/1‑250 (MQ=255)                                                         aaaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAt                                                                                                                                                                                           <  1:84771/250‑1 (MQ=255)                                                          aaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                         >  2:119164/1‑251 (MQ=255)                                                               ggTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaa                                                                                                                                                                                    <  1:364423/251‑1 (MQ=255)                                                                    ccTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCg                                                                                                                                                                               >  1:296894/1‑251 (MQ=255)                                                                    ccTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCg                                                                                                                                                                               <  1:194250/251‑1 (MQ=255)                                                                                    aaTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGt                                                                                                                                                                <  2:75160/250‑1 (MQ=255)                                                                                     aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTGAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTa                                                                                                                                                              <  1:177294/251‑1 (MQ=255)                                                                                       ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                            <  2:315070/251‑1 (MQ=255)                                                                                       ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                            >  1:40789/1‑251 (MQ=255)                                                                                                 gcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCGTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTTTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTg                                                                                                                                                  <  2:285605/251‑1 (MQ=255)                                                                                                    aaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                >  1:9416/1‑250 (MQ=255)                                                                                                     accgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAt                                                                                                                                              <  1:119164/251‑1 (MQ=255)                                                                                                          gaactgtagacattaattgTGATTATCTCATNAATGTAATCGAAAATTCTTACAACAACGGGTGTGTTGAGTTTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTTCGTTGGGTGGTTGCTTTCCGTGAAATCTGTA‑CGCAATTCAGAGAAGGCTTTTTTAGGCCTATAAGTGGTCATGGGATCTTGTTGTCCGAATTCAGGAAAGGCGAGATTTACAAATGGTTAATTATGAAACTGGGaggtatt                                                                                                                                         <  2:216494/240‑7 (MQ=255)                                                                                                           aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGCAAATTCTGACTACAACGGTTAGGTTGGGTGTGAATATAAACCCCAAACCACCACGGCAGCCGGTTTACGCTGGATGGATCCGTAACGTTAAAAC‑GTGACGCTATTCTGACAATGCTTTTTTAGGCCGCTAAGTAGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTCTACAACTGGGATTTATTg                                                                                                                                        >  2:439327/1‑251 (MQ=255)                                                                                                                            aTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagt                                                                                                                       >  1:245724‑M1/1‑246 (MQ=255)                                                                                                                                   tctcTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggc                                                                                                                >  1:483866‑M1/1‑239 (MQ=255)                                                                                                                                          aaGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGGTGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                         <  1:428071‑M1/251‑20 (MQ=255)                                                                                                                                           aGGTAATCGAAAATTCTGACTACAAAGGTAGGATTGGGTGTGAATATCAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACAGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGACCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGTATTAgtagtagtaggcattagtc                                                                                                         >  1:411835‑M1/1‑231 (MQ=255)                                                                                                                                               aaTCGAAAAATCTGACTAAAACGTTTGGGTTGGGTGTGAATATAAACCCCAAACCTCCACGGAAGACGTTTTACGCTGGATGGATTCGTCCCGGTAAAAC‑GTGACGCTATTCAGACTATGCTTTTTTAAGCCGCTTAGTTGGCAGGGGATCGTGTTGTCCGAATACAGTAAAATCGAAAGTTAAAAGAGGTTAATTATGAAACTGAGATTTATTGTCTTAGGCATTagtagtcgtaggcattagacatttg                                                                                                    <  1:208130/251‑25 (MQ=255)                                                                                                                                                          cTGACTACAACGGATGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATAGTGTTGTCCGAATTCAGGAATAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTCGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcg                                                                                         >  2:160320‑M1/1‑216 (MQ=255)                                                                                                                                                                aCAACGGTTGGGTTGGGTGTGAATATAACCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgctt                                                                                   <  1:98967‑M1/251‑42 (MQ=255)                                                                                                                                                                         gggttgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtct                                                                          <  1:76773‑M1/251‑51 (MQ=255)                                                                                                                                                                             tgggtGTGAATATAAACCCCAATCCACCACTGTAGCCGGGTTACGCTGGATGGATCCGTACCGTTAAAAC‑GGTACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATGGATTGGCTTATGCATTAgtagtcgtagacattagacatttgttccttatgcgacttgtttcgcgtcttat                                                                       <  2:316517‑M1/250‑54 (MQ=255)                                                                                                                                                                             tgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttat                                                                       <  2:9416‑M1/250‑54 (MQ=255)                                                                                                                                                                                                 aaaCCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgc                                                  >  1:443913‑M1/1‑177 (MQ=255)                                                                                                                                                                                                            gAAGCCTGTTTACGATGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATGCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttataaggcgtacaaccggtgccgcatccggcaatt                                       <  1:264574‑M1/251‑86 (MQ=255)                                                                                                                                                                                                             aaGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcctccggcaattg                                      >  1:293850‑M1/1‑165 (MQ=255)                                                                                                                                                                                                                 cGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattag                                                                                                           <  1:86457‑M1/178‑18 (MQ=255)                                                                                                                                                                                                                 cGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattag                                                                                                           >  2:86457‑M1/1‑161 (MQ=255)                                                                                                                                                                                                                   gTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcac                                >  2:159800‑M1/1‑159 (MQ=255)                                                                                                                                                                                                                     ttACGCTGGATGGATCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcaca                               <  2:100975‑M1/250‑94 (MQ=255)                                                                                                                                                                                                                                   tCCGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGGAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggagcacaatgcctgatgcgatg                >  1:311083‑M1/1‑143 (MQ=255)                                                                                                                                                                                                                                     cGTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgct              <  2:296894‑M1/251‑111 (MQ=255)                                                                                                                                                                                                                                      gTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt             >  2:141869‑M1/1‑140 (MQ=255)                                                                                                                                                                                                                                      gTACCGTTAAAAC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt             >  2:347236‑M1/1‑140 (MQ=255)                                                                                                                                                                                                                                              aaaaC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcat      <  1:455689‑M1/250‑119 (MQ=255)                                                                                                                                                                                                                                                 aC‑GTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttt  >  1:266241‑M1/1‑129 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAAC‑GTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAG  >  NC_000913/536248‑536738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑