Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,517	T→C	A84A (GCA→GCG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,517	0	T	C	100.0%	143.8 / NA	44	A84A (GCA→GCG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/36);  total (8/36)

AAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCC‑ATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTG  >  NC_000913/3815276‑3815756                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                aaaTCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGGCAGGTCATGATGCTCCGCCCGCGCcacgggg                                                                                                                                                                                                                                        >  1:228067/1‑247 (MQ=255)                           cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCttt                                                                                                                                                                                                              <  1:100923/251‑1 (MQ=255)                             atcaaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGt                                                                                                                                                                                                            <  1:36864/247‑1 (MQ=255)                                   cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAg                                                                                                                                                                                                      <  1:61057/251‑1 (MQ=255)                                   cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTCTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCGCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAg                                                                                                                                                                                                      <  1:397270/251‑1 (MQ=255)                                      aatcaatccGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGc                                                                                                                                                                                                   <  2:317140/243‑1 (MQ=255)                                         caatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAACCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCaa                                                                                                                                                                                                <  2:177370/247‑1 (MQ=255)                                             ccGCCAGCAAGCGTCGCGCCATGAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGTTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTTAGCAGGGCCAAAca                                                                                                                                                                                             <  2:429597/249‑1 (MQ=255)                                                      aGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTGCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTGTGTAAGCAGGGCCAAACAGCAGATCGaa                                                                                                                                                                                   <  1:396111/251‑1 (MQ=255)                                                           cgcgCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCaa                                                                                                                                                                              <  1:149029/251‑1 (MQ=255)                                                            ccgccatgagCCTGAATAA‑CTCCCATCGATTCTCGAATCTCCGTGCCGGCGGTGGTCACACCATCTACCAGCATTCCGCGTCCTT‑TTACGTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATGGGGATCCCTTTGTAAGCATGGCCAAACTGCAGAGCGAACTCAAt                                                                                                                                                                             <  2:475419/242‑1 (MQ=255)                                                                       cTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCCCCGCCAGCGCCACGGCGGGAGGGGGCGCCAACGGGGTCCCTTTGTAAGCAGGGGCAAAACGCAGATAGAACTccattgcagaatccc                                                                                                                                                                  >  1:171302/1‑237 (MQ=255)                                                                        tGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGaa                                                                                                                                                                                   <  2:356827/233‑1 (MQ=255)                                                                         gAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCTGTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATTCCAGAATcctcc                                                                                                                                                                <  2:257230/251‑4 (MQ=255)                                                                                  cc‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCCATCGGGATCCCCTTTTAAACAGGGCCAAACAGCAGAACGAACTCAACTCCAGAATTCACCAAAGCTTc                                                                                                                                                        >  1:377798/1‑250 (MQ=255)                                                                                           ccgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCGCCGCGAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTGTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAATGCTTCAGCGTaaaa                                                                                                                                               <  2:439511/250‑1 (MQ=255)                                                                                               gAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCaa                                                                                                                                                                                                <  2:75943/198‑1 (MQ=255)                                                                                                   cGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCt                                                                                                                                        <  1:258214/250‑1 (MQ=255)                                                                                                         tCCGTCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGt                                                                                                                                  <  1:74729/250‑1 (MQ=255)                                                                                                             gcgttgttcatctcagctcccAGCATGACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCTTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCTCCGCCAGCGCCACGGCTGTAGTGTTCGCAATCGGGATCCCTTTATAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCGTAAATACTGCCTAACAGGGCCAg                                                                                                                             <  2:402945/232‑1 (MQ=255)                                                                                                                gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAggtc                                                                                                                          <  1:60538/251‑4 (MQ=255)                                                                                                                      ccatcatCTACCAGCATTACGAGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTTAGGATGCTCCGCCAGCGCCACGGCAGTAGTGGGCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGccc                                                                                                                    <  2:356393/250‑1 (MQ=255)                                                                                                                             cTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGGTCCCTTTGTAAGCAGGGCCAAACAGCAGATCCAACTCAATGCCAGAATCCACCAATGCTTCAACGTAAAAAAGGCCTAACAAGGCCAGGGCGCGCCCGGGAtt                                                                                                              >  1:262865/1‑250 (MQ=255)                                                                                                                               tcccgCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGACTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                             <  2:283666/245‑1 (MQ=255)                                                                                                                               aCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTAtt                                                                                                              <  1:456851/248‑1 (MQ=255)                                                                                                                               aCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTaa                                                                                                            <  1:274004/250‑1 (MQ=255)                                                                                                                                                 tttagcg‑cgCTCCCAACCAGAGTTCCGCCTTAAACGTGGTCTGTTGCCTATTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGTTGCGCCGGCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCGTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGCAATCCACAAATGCTACAGCGTAAAAACGGCCTAACAGTCCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaag                                                                                           <  2:358261/244‑1 (MQ=255)                                                                                                                                                 tGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCTTAAAAACGGCCTAACAGTGCCAGGTCGCGCCGGGTATTAAACAGCCCGGCGTTgaag                                                                                           <  2:435376/249‑1 (MQ=255)                                                                                                                                                      cg‑cgCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCCGAAACCACCCATGCTTCCGCGTAAAAACGGCCTAACAGTGCCAGGGCGCGCCCGGGATTAAACAGCCCGGCGTTGGAGAAATAgg                                                                                    >  1:183821/1‑251 (MQ=255)                                                                                                                                                           cTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTACAGCAGTACGGCAGGTAACGGTCAGGATGCTCCGCCAGCGCCCCGGCGGTAGTTGGCGCCATCCGGATCCCTTTTTAAGCAGGGGCAAAAAACAAATAGAAATCAAATCCAAAAACCACCAAAGCTTCAACGTCAAAAAGACCCAAAAATGCCAGGTCGCGCCCCGGATTAAACAACCCCGCCGTGAAGAAAtcagggct                                                                                >  1:248260/1‑244 (MQ=255)                                                                                                                                                             accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCaatg                                                                                                                                                            <  1:472985/173‑3 (MQ=255)                                                                                                                                                              ccaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGTCGCAATTGGGATCCCCTTGTAAGCAGGGCCAAACAGCCAAACGGAATCAAAGCCCGAATCCACCAATGCTTTAGCGGAAAAAAGGCCCAACAAGGCCAGGTCGCCCCCCGTATTAAACCACCCCGCGGTGAAGAAAAAGGGGGtttt                                                                             >  2:407299/1‑251 (MQ=255)                                                                                                                                                                 accaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCCTGATGCTCCGCCAGCGCCACGGCGGGAGGGGGCGCAATCCGGATCCCTTTGTAAGCAGGGGCAAAAAGCCGAACGGACTCAAAGCCAGAATCCCCCAATGGTTCAACGTAAAAACGGCCTAACAATGCCCGGTCGCCCCCCGGGTTAAACAGCCCCGCGtttgagaaaaaaggggtttttcc                                                                          >  1:74925/1‑230 (MQ=255)                                                                                                                                                                   caGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGcc                                                                        <  1:192732/251‑1 (MQ=255)                                                                                                                                                                         gccgccgcctccGTGGTCTTTTCCCTCTTTGCGGTTAAAGCAGTACTGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGTTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACACAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                                                                            <  2:197342/215‑1 (MQ=255)                                                                                                                                                                                                cctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATTATGCACCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACGCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACttt                                           <  2:278356/249‑1 (MQ=255)                                                                                                                                                                                                        cGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtg                                   <  1:89547/251‑1 (MQ=255)                                                                                                                                                                                                        cGGTTAAAGCAGTACGGCAGGTCAAGGTCAGGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtg                                   <  2:345057/251‑1 (MQ=255)                                                                                                                                                                                                            tAAAGGAGTACGTCAGGTCAAGGTGATGATGCTCCGCCAGCGCCACGGCGGTATTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCATATCGAACTCAATGCCAGAATCCACCAAGGCTTCAGCGTAAAAACGGCCTAACAGTTCCAGGTCGCGCCCGGTATTAAACAGCCCGGCTTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctg                                <  1:276761/250‑2 (MQ=255)                                                                                                                                                                                                                         caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                  <  2:183821/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACGTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAc          <  1:355585/251‑1 (MQ=255)                                                                                                                                                                                                                                  gtcatGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACt         <  2:168690/247‑1 (MQ=255)                                                                                                                                                                                                                                    catGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTgg       <  1:396520/249‑1 (MQ=255)                                                                                                                                                                                                                                          gCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg  <  1:83703/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                AAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCC‑ATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTG  >  NC_000913/3815276‑3815756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑