Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,466	T→C	E101E (GAA→GAG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,466	0	T	C	100.0%	176.1 / NA	51	E101E (GAA→GAG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/34);  total (17/34)

GTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCC‑ATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCC  >  NC_000913/3815221‑3815686                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             gTGATGATAGAGATCACTTTGCAGTTGGAAGCACGCTCAACTTCCTGAATCGCCGAAAGCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCttt                                                                                                                                                                                                                         <  2:36264/251‑1 (MQ=255)      gatAGAGATCACTTTGCAGTTGTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                                                                                                    <  1:122296/251‑1 (MQ=255)      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                                                                                                    <  1:87967/251‑1 (MQ=255)      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                                                                                                    <  1:468255/251‑1 (MQ=255)      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                                                                                                                                                                    >  1:435376/1‑251 (MQ=255)          gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCCGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                                                                                                                                                                                 >  1:283666/1‑250 (MQ=255)             aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGACTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGTTAAAGc                                                                                                                                                                                                              >  1:360322/1‑250 (MQ=255)                  ttttcAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCAGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAACCTGAATAATCTCC‑ATCGATTAGCGAATCGCCGTTCCGGCGGCGATCACAGCCTCTACCAGCTATACGCGACCTTCTTACG‑CGCTACCAACCAGAATGCCGCCTTCACCGTGGGCTTTTCCCTCttttctgtttactcagtacg                                                                                                                                                                                                        >  2:296712/5‑234 (MQ=255)                      cAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGc                                                                                                                                                                                                      >  1:345057/1‑249 (MQ=255)                        gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                                                                                                                                                                          <  2:470121/227‑1 (MQ=255)                        gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                                                                                                                                                                          >  1:470121/1‑227 (MQ=255)                            tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTTCGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGGGGGCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGccgggcaa                                                                                                                                                                                               >  2:83703/1‑243 (MQ=255)                                 ccGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGcaggtcaaggtcat                                                                                                                                                                                         <  1:152678/250‑3 (MQ=255)                                       aaCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCt                                                                                                                                                                                   <  1:17763/251‑1 (MQ=255)                                           tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCACGCGCCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCCGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTTAAGCAGTACGGCAGGTCAAGGTCATGATCCTCCGc                                                                                                                                                                               >  1:439511/1‑251 (MQ=255)                                            ccTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCCGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGGTAAAGAAGTACGGcagggtaagggcctggatgcacccc                                                                                                                                                                              >  2:60538/1‑230 (MQ=255)                                                aaTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAgcg                                                                                                                                                                          >  1:349876/1‑249 (MQ=255)                                                        aaaTCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGGCAGGTCATGATGCTCCGCCCGCGCcacgggg                                                                                                                                                                  >  1:228067/1‑247 (MQ=255)                                                        aaaTCTCGCCGCGCCCCCGGTCCTGACGAGCAAGGGAAATCAATACGCCAGCAAGGGTCGCGCGATGGGGCTGAGTAGTCGCC‑AGCGATGCGCGAATCGCCGTTCGGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAGCG‑CGCTACCAGCCAGATGGCCGCCTTCACCGGGGTCTTGTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGgtggtggtggtcggggggggcggcgggg                                                                                                                                                                  <  2:158919/251‑23 (MQ=255)                                                                                  cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCttt                                                                                                                                        <  1:100923/251‑1 (MQ=255)                                                                                    atcaaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGt                                                                                                                                      <  1:36864/247‑1 (MQ=255)                                                                                          cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAg                                                                                                                                <  1:61057/251‑1 (MQ=255)                                                                                          cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTCTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCGCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAg                                                                                                                                <  1:397270/251‑1 (MQ=255)                                                                                             aatcaatccGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGc                                                                                                                             <  2:317140/243‑1 (MQ=255)                                                                                                caatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAACCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCaa                                                                                                                          <  2:177370/247‑1 (MQ=255)                                                                                                    ccGCCAGCAAGCGTCGCGCCATGAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGTTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTTAGCAGGGCCAAAca                                                                                                                       <  2:429597/249‑1 (MQ=255)                                                                                                             aGCGTCGCGCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTGCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTGTGTAAGCAGGGCCAAACAGCAGATCGaa                                                                                                             <  1:396111/251‑1 (MQ=255)                                                                                                                  cgcgCCATTAGCCTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCaa                                                                                                        <  1:149029/251‑1 (MQ=255)                                                                                                                   ccgccatgagCCTGAATAA‑CTCCCATCGATTCTCGAATCTCCGTGCCGGCGGTGGTCACACCATCTACCAGCATTCCGCGTCCTT‑TTACGTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATGGGGATCCCTTTGTAAGCATGGCCAAACTGCAGAGCGAACTCAAt                                                                                                       <  2:475419/242‑1 (MQ=255)                                                                                                                              cTGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCCCCGCCAGCGCCACGGCGGGAGGGGGCGCCAACGGGGTCCCTTTGTAAGCAGGGGCAAAACGCAGATAGAACTccattgcagaatccc                                                                                            >  1:171302/1‑237 (MQ=255)                                                                                                                               tGAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGaa                                                                                                             <  2:356827/233‑1 (MQ=255)                                                                                                                                gAATAATCTCC‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCTGTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATTCCAGAATcctcc                                                                                          <  2:257230/251‑4 (MQ=255)                                                                                                                                         cc‑ATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCCATCGGGATCCCCTTTTAAACAGGGCCAAACAGCAGAACGAACTCAACTCCAGAATTCACCAAAGCTTc                                                                                  >  1:377798/1‑250 (MQ=255)                                                                                                                                                  ccgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCGCCGCGAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTGTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAATGCTTCAGCGTaaaa                                                                         <  2:439511/250‑1 (MQ=255)                                                                                                                                                      gAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCaa                                                                                                                          <  2:75943/198‑1 (MQ=255)                                                                                                                                                          cGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCt                                                                  <  1:258214/250‑1 (MQ=255)                                                                                                                                                                tCCGTCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGt                                                            <  1:74729/250‑1 (MQ=255)                                                                                                                                                                    gcgttgttcatctcagctcccAGCATGACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCTTGGTCTTTTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCTCCGCCAGCGCCACGGCTGTAGTGTTCGCAATCGGGATCCCTTTATAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCGTAAATACTGCCTAACAGGGCCAg                                                       <  2:402945/232‑1 (MQ=255)                                                                                                                                                                       gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAggtc                                                    <  1:60538/251‑4 (MQ=255)                                                                                                                                                                             ccatcatCTACCAGCATTACGAGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTTAGGATGCTCCGCCAGCGCCACGGCAGTAGTGGGCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGccc                                              <  2:356393/250‑1 (MQ=255)                                                                                                                                                                                    cTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGGTCCCTTTGTAAGCAGGGCCAAACAGCAGATCCAACTCAATGCCAGAATCCACCAATGCTTCAACGTAAAAAAGGCCTAACAAGGCCAGGGCGCGCCCGGGAtt                                        >  1:262865/1‑250 (MQ=255)                                                                                                                                                                                      tcccgCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGACTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                       <  2:283666/245‑1 (MQ=255)                                                                                                                                                                                      aCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTAtt                                        <  1:456851/248‑1 (MQ=255)                                                                                                                                                                                      aCCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTaa                                      <  1:274004/250‑1 (MQ=255)                                                                                                                                                                                                        tttagcg‑cgCTCCCAACCAGAGTTCCGCCTTAAACGTGGTCTGTTGCCTATTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGTTGCGCCGGCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCGTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGCAATCCACAAATGCTACAGCGTAAAAACGGCCTAACAGTCCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaag                     <  2:358261/244‑1 (MQ=255)                                                                                                                                                                                                        tGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGGAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCTTAAAAACGGCCTAACAGTGCCAGGTCGCGCCGGGTATTAAACAGCCCGGCGTTgaag                     <  2:435376/249‑1 (MQ=255)                                                                                                                                                                                                             cg‑cgCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCCGAAACCACCCATGCTTCCGCGTAAAAACGGCCTAACAGTGCCAGGGCGCGCCCGGGATTAAACAGCCCGGCGTTGGAGAAATAgg              >  1:183821/1‑251 (MQ=255)                                                                                                                                                                                                                  cTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTACAGCAGTACGGCAGGTAACGGTCAGGATGCTCCGCCAGCGCCCCGGCGGTAGTTGGCGCCATCCGGATCCCTTTTTAAGCAGGGGCAAAAAACAAATAGAAATCAAATCCAAAAACCACCAAAGCTTCAACGTCAAAAAGACCCAAAAATGCCAGGTCGCGCCCCGGATTAAACAACCCCGCCGTGAAGAAAtcagggct          >  1:248260/1‑244 (MQ=255)                                                                                                                                                                                                                    accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCaatg                                                                                      <  1:472985/173‑3 (MQ=255)                                                                                                                                                                                                                     ccaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGTCGCAATTGGGATCCCCTTGTAAGCAGGGCCAAACAGCCAAACGGAATCAAAGCCCGAATCCACCAATGCTTTAGCGGAAAAAAGGCCCAACAAGGCCAGGTCGCCCCCCGTATTAAACCACCCCGCGGTGAAGAAAAAGGGGGtttt       >  2:407299/1‑251 (MQ=255)                                                                                                                                                                                                                        accaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCCTGATGCTCCGCCAGCGCCACGGCGGGAGGGGGCGCAATCCGGATCCCTTTGTAAGCAGGGGCAAAAAGCCGAACGGACTCAAAGCCAGAATCCCCCAATGGTTCAACGTAAAAACGGCCTAACAATGCCCGGTCGCCCCCCGGGTTAAACAGCCCCGCGtttgagaaaaaaggggtttttcc    >  1:74925/1‑230 (MQ=255)                                                                                                                                                                                                                          caGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGcc  <  1:192732/251‑1 (MQ=255)                                                                                                                                                                                                                                gccgccgcctccGTGGTCTTTTCCCTCTTTGCGGTTAAAGCAGTACTGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGTTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACACAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                      <  2:197342/215‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             GTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCC‑ATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACG‑CGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCC  >  NC_000913/3815221‑3815686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑