Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,307	G→A	L154L (CTC→CTT)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,307	0	G	A	100.0%	205.4 / NA	59	L154L (CTC→CTT)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/26);  total (33/26)

GCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTT  >  NC_000913/3815063‑3815552                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGCG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGCTGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCCGATCTTTCAGAGTGATGATAGAGATCCCTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCATGACGATCAAGCGa                                                                                                                                                                                                                                                  >  2:36864/1‑250 (MQ=255) aacGCTAATTGAGGCACAGTTGCAGTAATATGACGCCGGGTGACATTTCATCCGGCGCG‑TTTCTTTATACGCCAAAATCTTCGCGATATGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTAAAGATAGGCAATCAGATCTTTCATGGTGATGCTAGAGATCCCTTTGCAGTTGTAATAACGCTCAACTTCCTGACTCGCCGAAATCTCGCCGCGCCCCCGTTCATGCCGATCAAGCGa                                                                                                                                                                                                                                                  >  2:276761/3‑250 (MQ=255)    gCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCTCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAAt                                                                                                                                                                                                                                               >  1:140440/1‑250 (MQ=255)        atggCGGCACAGTTGCAGTAAAATGCCGCCGTATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTTTTGGCGATAGGCCTTAACCTCCTCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCaata                                                                                                                                                                                                                                          <  2:267491/248‑3 (MQ=255)                           aaTATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGGGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGACGCGcc                                                                                                                                                                                                                       >  2:89547/1‑251 (MQ=255)                                gactccggatagcTTTTCATCCGGCGAT‑TTTCTTTACACGCCCAACTCTTCGCGATAGGCCTTAACCGCAGCCAGATGTTCAGCCATCTCCGGCTTCTCTTGCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAAGCTAGCCGAGCCCCCGTTCCTGACGAACAAGCGACATCAATACGCCAGCAAGCTTCGCGCCAtt                                                                                                                                                                                                                    >  2:445219/13‑249 (MQ=255)                                         tGACTTTTCATCCG‑TGAGGTTTCTTTAAACGCTAAACTCGCCGGGAGAGGCCTTACCCGCCGCAAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGaata                                                                                                                                                                                                          <  2:163479/250‑1 (MQ=255)                                            cTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtct                                                                                                                                                                                                      >  1:278356/1‑251 (MQ=255)                                             ttttCATCCGGCGAG‑TTTCTTTAAAAGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtctc                                                                                                                                                                                                     >  1:339336/1‑251 (MQ=255)                                               ttCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTcc                                                                                                                                                                                                    >  1:317140/1‑250 (MQ=255)                                                 cATCCGGCGAG‑TTTCGTTAAACGCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGCTAGGCAATCAGCTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCGGCGCCCCCGTTCCTGACGATCAAGCGAAATCAAAACGCCAGCAAGCGTCGCGCCATTAGCCTGAAAAATCTcctcc                                                                                                                                                                                                 >  2:355585/1‑248 (MQ=255)                                                         gAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCATCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTcgcg                                                                                                                                                                                         <  1:126951/251‑1 (MQ=255)                                                                 ttaactccacaacctctTCGCGATGTGTCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTACTCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCCATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGc                                                                                                                                                                                   <  2:251352/237‑1 (MQ=255)                                                                   caacccccaaacccttccgaaTAGGCCTTACCCGCCGCCAGAGGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCACCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                                                 <  2:160803/230‑1 (MQ=255)                                                                      aCGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGAGCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTcc                                                                                                                                                                             <  2:95855/251‑1 (MQ=255)                                                                        cccAAACTCTACGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCcgg                                                                                                                                                                           <  1:363546/250‑1 (MQ=255)                                                                           aatctctTCGCGAGAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCGCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAAGCGCCGAAATCGCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCAGCGATTCGCGAATCGCCGTTCcggcgg                                                                                                                                                                        <  2:97337/248‑1 (MQ=255)                                                                              ctctTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTg                                                                                                                                                                      >  1:356393/1‑250 (MQ=255)                                                                                  tCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATcac                                                                                                                                                                 >  2:152678/1‑251 (MQ=255)                                                                                           gCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTAc                                                                                                                                                        <  1:22246/251‑1 (MQ=255)                                                                                             cTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATc                                                                                                                                                                   <  1:113953/238‑1 (MQ=255)                                                                                             cTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATc                                                                                                                                                                   >  2:113953/1‑238 (MQ=255)                                                                                                    gccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCATCTACCAGCAttcc                                                                                                                                               >  2:74729/1‑249 (MQ=255)                                                                                                    gccgccAGAGGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAc                                                                                                                                               <  2:130700/251‑1 (MQ=255)                                                                                                        ccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGt                                                                                                                                           <  1:481731/251‑1 (MQ=255)                                                                                                               ttacGCCATTTCCGACTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGAAGATAGAGATCAATTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTACATGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCACCATTAGCCTGACTAATCTCCATCgcttc                                                                                                                                                                                            >  1:448458/4‑191 (MQ=255)                                                                                                               ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGattc                                                                                                                                                                                            >  1:448454/1‑192 (MQ=255)                                                                                                               ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGattc                                                                                                                                                                                            <  2:448454/195‑4 (MQ=255)                                                                                                               ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGt                                                                                                                                     >  1:168690/1‑250 (MQ=255)                                                                                                                  cgctaTTTCCGGGTTCTCTTCCAGATACGCAATCAGATCTTTCAGGGTGATGATAGACAACACTTTGCAGTTGTAATCACGCTCAACTTGCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAACCCAATACTCCAGCAACCGTCGCGCCATTAGCCTGACTCATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGGACACATCATATACCCGCATTACCCGTccacgtaa                                                                                                                                   >  2:482581/5‑243 (MQ=255)                                                                                                                    ccATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAAcgcc                                                                                                                               >  1:158919/1‑250 (MQ=255)                                                                                                                        ttCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTa                                                                                                                            <  1:146978/250‑1 (MQ=255)                                                                                                                             gCTTCTCTTCCAGATAGCCAATCAGCTCTTTAAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCAAGCTCAACTTCCTGAATCGACGAAATCTCGCCGCGCACCCGTTCCTGACGATCAAGCGAACTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCCACGTTCCGGCGGTGATCCCCTCATCTAACAGCATTACGCCTCCTTGTAACGCGCTaccaacc                                                                                                                      >  2:274004/1‑251 (MQ=255)                                                                                                                                           aaggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAATCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTc                                                                                                        <  2:146079/248‑1 (MQ=255)                                                                                                                                            aggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTcc                                                                                                       >  2:258214/3‑250 (MQ=255)                                                                                                                                                 aTCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCATCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTg                                                                                                  <  1:73052/251‑1 (MQ=255)                                                                                                                                                   cagatCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCACCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTgg                                                                                                 <  2:421247/246‑1 (MQ=255)                                                                                                                                                        cTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTAACAACCAGATTGCCGCCTTCACCGTGGTCtttt                                                                                           >  1:350526/1‑251 (MQ=255)                                                                                                                                                             aGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATAATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctc                                                                                      >  1:390435/1‑248 (MQ=255)                                                                                                                                                                gTGATGATAGAGATCACTTTGCAGTTGGAAGCACGCTCAACTTCCTGAATCGCCGAAAGCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCttt                                                                                   <  2:36264/251‑1 (MQ=255)                                                                                                                                                                     gatAGAGATCACTTTGCAGTTGTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                              <  1:122296/251‑1 (MQ=255)                                                                                                                                                                     gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGt                                                                              <  1:87967/251‑1 (MQ=255)                                                                                                                                                                     gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                              >  1:435376/1‑251 (MQ=255)                                                                                                                                                                     gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                                              <  1:468255/251‑1 (MQ=255)                                                                                                                                                                         gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCCGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                                           >  1:283666/1‑250 (MQ=255)                                                                                                                                                                            aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGACTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGTTAAAGc                                                                        >  1:360322/1‑250 (MQ=255)                                                                                                                                                                                 ttttcAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCAGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAACCTGAATAATCTCCATCGATTAGCGAATCGCCGTTCCGGCGGCGATCACAGCCTCTACCAGCTATACGCGACCTTCTTACGCGCTACCAACCAGAATGCCGCCTTCACCGTGGGCTTTTCCCTCttttctgtttactcagtacg                                                                  >  2:296712/5‑234 (MQ=255)                                                                                                                                                                                     cAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGc                                                                >  1:345057/1‑249 (MQ=255)                                                                                                                                                                                       gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                                    >  1:470121/1‑227 (MQ=255)                                                                                                                                                                                       gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                                                    <  2:470121/227‑1 (MQ=255)                                                                                                                                                                                           tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGGGGGCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGccgggcaa                                                         >  2:83703/1‑243 (MQ=255)                                                                                                                                                                                                ccGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGcaggtcaaggtcat                                                   <  1:152678/250‑3 (MQ=255)                                                                                                                                                                                                      aaCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCt                                             <  1:17763/251‑1 (MQ=255)                                                                                                                                                                                                          tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCACGCGCCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCCGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTTAAGCAGTACGGCAGGTCAAGGTCATGATCCTCCGc                                         >  1:439511/1‑251 (MQ=255)                                                                                                                                                                                                           ccTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCCGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGGTAAAGAAGTACGGcagggtaagggcctggatgcacccc                                        >  2:60538/1‑230 (MQ=255)                                                                                                                                                                                                               aaTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAgcg                                    >  1:349876/1‑249 (MQ=255)                                                                                                                                                                                                                       aaaTCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGGCAGGTCATGATGCTCCGCCCGCGCcacgggg                            >  1:228067/1‑247 (MQ=255)                                                                                                                                                                                                                       aaaTCTCGCCGCGCCCCCGGTCCTGACGAGCAAGGGAAATCAATACGCCAGCAAGGGTCGCGCGATGGGGCTGAGTAGTCGCCAGCGATGCGCGAATCGCCGTTCGGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAGCGCGCTACCAGCCAGATGGCCGCCTTCACCGGGGTCTTGTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGgtggtggtggtcggggggggcggcgggg                            <  2:158919/251‑23 (MQ=255)                                                                                                                                                                                                                                                 cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCttt  <  1:100923/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTT  >  NC_000913/3815063‑3815552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑