Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,292	G→C	R159R (CGC→CGG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,292	0	G	C	100.0%	226.7 / NA	63	R159R (CGC→CGG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (33/30);  total (33/30)

GATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCG  >  NC_000913/3815046‑3815526                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gatTTTGCCGCCCGCGGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAT‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGtt                                                                                                                                                                                                                                        <  2:122974/251‑1 (MQ=255)     ttGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCt                                                                                                                                                                                                                                     <  1:357445/250‑1 (MQ=255)          gCCCGCGGGCCGCTAATTGCGGCACAGTTGCATTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGAt                                                                                                                                                                                                                               <  1:296825/251‑1 (MQ=255)          ccccGCTGGCCGCTAATTGCTGCACGGTTGCAGTAATATGACGCCGGATGACTTTCCATCCGGCGAG‑TTTCTTTAAACGCCAACCTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGAt                                                                                                                                                                                                                               <  2:293824/250‑1 (MQ=255)           cccGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATc                                                                                                                                                                                                                              <  1:49258/251‑1 (MQ=255)                  gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGCG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGCTGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCCGATCTTTCAGAGTGATGATAGAGATCCCTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCATGACGATCAAGCGa                                                                                                                                                                                                                        >  2:36864/1‑250 (MQ=255)                  aacGCTAATTGAGGCACAGTTGCAGTAATATGACGCCGGGTGACATTTCATCCGGCGCG‑TTTCTTTATACGCCAAAATCTTCGCGATATGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTAAAGATAGGCAATCAGATCTTTCATGGTGATGCTAGAGATCCCTTTGCAGTTGTAATAACGCTCAACTTCCTGACTCGCCGAAATCTCGCCGCGCCCCCGTTCATGCCGATCAAGCGa                                                                                                                                                                                                                        >  2:276761/3‑250 (MQ=255)                     gCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCTCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAAt                                                                                                                                                                                                                     >  1:140440/1‑250 (MQ=255)                         atggCGGCACAGTTGCAGTAAAATGCCGCCGTATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTTTTGGCGATAGGCCTTAACCTCCTCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCaata                                                                                                                                                                                                                <  2:267491/248‑3 (MQ=255)                                            aaTATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGGGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGACGCGcc                                                                                                                                                                                             >  2:89547/1‑251 (MQ=255)                                                 gactccggatagcTTTTCATCCGGCGAT‑TTTCTTTACACGCCCAACTCTTCGCGATAGGCCTTAACCGCAGCCAGATGTTCAGCCATCTCCGGCTTCTCTTGCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAAGCTAGCCGAGCCCCCGTTCCTGACGAACAAGCGACATCAATACGCCAGCAAGCTTCGCGCCAtt                                                                                                                                                                                          >  2:445219/13‑249 (MQ=255)                                                          tGACTTTTCATCCG‑TGAGGTTTCTTTAAACGCTAAACTCGCCGGGAGAGGCCTTACCCGCCGCAAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGaata                                                                                                                                                                                <  2:163479/250‑1 (MQ=255)                                                             cTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtct                                                                                                                                                                            >  1:278356/1‑251 (MQ=255)                                                              ttttCATCCGGCGAG‑TTTCTTTAAAAGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAAtctc                                                                                                                                                                           >  1:339336/1‑251 (MQ=255)                                                                ttCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTcc                                                                                                                                                                          >  1:317140/1‑250 (MQ=255)                                                                  cATCCGGCGAG‑TTTCGTTAAACGCCAAACTCTTCGCGATAGGCCTTACCCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGCTAGGCAATCAGCTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCGGCGCCCCCGTTCCTGACGATCAAGCGAAATCAAAACGCCAGCAAGCGTCGCGCCATTAGCCTGAAAAATCTcctcc                                                                                                                                                                       >  2:355585/1‑248 (MQ=255)                                                                          gAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCATCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTcgcg                                                                                                                                                               <  1:126951/251‑1 (MQ=255)                                                                                  ttaactccacaacctctTCGCGATGTGTCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTACTCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCCATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGc                                                                                                                                                         <  2:251352/237‑1 (MQ=255)                                                                                    caacccccaaacccttccgaaTAGGCCTTACCCGCCGCCAGAGGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCACCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCg                                                                                                                                                       <  2:160803/230‑1 (MQ=255)                                                                                       aCGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGAGCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTcc                                                                                                                                                   <  2:95855/251‑1 (MQ=255)                                                                                         cccAAACTCTACGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCcgg                                                                                                                                                 <  1:363546/250‑1 (MQ=255)                                                                                            aatctctTCGCGAGAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCGCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAAGCGCCGAAATCGCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCAGCGATTCGCGAATCGCCGTTCcggcgg                                                                                                                                              <  2:97337/248‑1 (MQ=255)                                                                                               ctctTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTg                                                                                                                                            >  1:356393/1‑250 (MQ=255)                                                                                                   tCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATcac                                                                                                                                       >  2:152678/1‑251 (MQ=255)                                                                                                            gCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTAc                                                                                                                              <  1:22246/251‑1 (MQ=255)                                                                                                              cTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATc                                                                                                                                         <  1:113953/238‑1 (MQ=255)                                                                                                              cTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATc                                                                                                                                         >  2:113953/1‑238 (MQ=255)                                                                                                                     gccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCATCTACCAGCAttcc                                                                                                                     >  2:74729/1‑249 (MQ=255)                                                                                                                     gccgccAGAGGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAc                                                                                                                     <  2:130700/251‑1 (MQ=255)                                                                                                                         ccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGt                                                                                                                 <  1:481731/251‑1 (MQ=255)                                                                                                                                ttacGCCATTTCCGACTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGAAGATAGAGATCAATTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTACATGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCACCATTAGCCTGACTAATCTCCATCgcttc                                                                                                                                                                  >  1:448458/4‑191 (MQ=255)                                                                                                                                ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGattc                                                                                                                                                                  >  1:448454/1‑192 (MQ=255)                                                                                                                                ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGattc                                                                                                                                                                  <  2:448454/195‑4 (MQ=255)                                                                                                                                ttCCGCCATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGt                                                                                                           >  1:168690/1‑250 (MQ=255)                                                                                                                                   cgctaTTTCCGGGTTCTCTTCCAGATACGCAATCAGATCTTTCAGGGTGATGATAGACAACACTTTGCAGTTGTAATCACGCTCAACTTGCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAACCCAATACTCCAGCAACCGTCGCGCCATTAGCCTGACTCATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGCGGACACATCATATACCCGCATTACCCGTccacgtaa                                                                                                         >  2:482581/5‑243 (MQ=255)                                                                                                                                     ccATTTCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAAcgcc                                                                                                     >  1:158919/1‑250 (MQ=255)                                                                                                                                         ttCCGGCTTCTCTTCCAGATAGGCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTa                                                                                                  <  1:146978/250‑1 (MQ=255)                                                                                                                                              gCTTCTCTTCCAGATAGCCAATCAGCTCTTTAAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCAAGCTCAACTTCCTGAATCGACGAAATCTCGCCGCGCACCCGTTCCTGACGATCAAGCGAACTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCCACGTTCCGGCGGTGATCCCCTCATCTAACAGCATTACGCCTCCTTGTAACGCGCTaccaacc                                                                                            >  2:274004/1‑251 (MQ=255)                                                                                                                                                            aaggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAATCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTc                                                                              <  2:146079/248‑1 (MQ=255)                                                                                                                                                             aggCAATCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTcc                                                                             >  2:258214/3‑250 (MQ=255)                                                                                                                                                                  aTCAGATCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCATCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTg                                                                        <  1:73052/251‑1 (MQ=255)                                                                                                                                                                    cagatCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCACCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTgg                                                                       <  2:421247/246‑1 (MQ=255)                                                                                                                                                                         cTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTAACAACCAGATTGCCGCCTTCACCGTGGTCtttt                                                                 >  1:350526/1‑251 (MQ=255)                                                                                                                                                                              aGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATAATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctc                                                            >  1:390435/1‑248 (MQ=255)                                                                                                                                                                                 gTGATGATAGAGATCACTTTGCAGTTGGAAGCACGCTCAACTTCCTGAATCGCCGAAAGCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCttt                                                         <  2:36264/251‑1 (MQ=255)                                                                                                                                                                                      gatAGAGATCACTTTGCAGTTGTAATCCCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                    <  1:122296/251‑1 (MQ=255)                                                                                                                                                                                      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGt                                                    <  1:87967/251‑1 (MQ=255)                                                                                                                                                                                      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                    >  1:435376/1‑251 (MQ=255)                                                                                                                                                                                      gatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGt                                                    <  1:468255/251‑1 (MQ=255)                                                                                                                                                                                          gagaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACCTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCCGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTaa                                                 >  1:283666/1‑250 (MQ=255)                                                                                                                                                                                             aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGACTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGTTAAAGc                                              >  1:360322/1‑250 (MQ=255)                                                                                                                                                                                                  ttttcAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCAGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAACCTGAATAATCTCCATCGATTAGCGAATCGCCGTTCCGGCGGCGATCACAGCCTCTACCAGCTATACGCGACCTTCTTACGCGCTACCAACCAGAATGCCGCCTTCACCGTGGGCTTTTCCCTCttttctgtttactcagtacg                                        >  2:296712/5‑234 (MQ=255)                                                                                                                                                                                                      cAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGc                                      >  1:345057/1‑249 (MQ=255)                                                                                                                                                                                                        gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                          >  1:470121/1‑227 (MQ=255)                                                                                                                                                                                                        gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGcctctt                                                          <  2:470121/227‑1 (MQ=255)                                                                                                                                                                                                            tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCCTCTACCAGCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGGGGGCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGccgggcaa                               >  2:83703/1‑243 (MQ=255)                                                                                                                                                                                                                 ccGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGcaggtcaaggtcat                         <  1:152678/250‑3 (MQ=255)                                                                                                                                                                                                                       aaCTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCt                   <  1:17763/251‑1 (MQ=255)                                                                                                                                                                                                                           tCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCACGCGCCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATCTACCCGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTTAAGCAGTACGGCAGGTCAAGGTCATGATCCTCCGc               >  1:439511/1‑251 (MQ=255)                                                                                                                                                                                                                            ccTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCCGATTGCCGCCTTCACCGGGGTCTTTTGCCTCTTTGCGGGTAAAGAAGTACGGcagggtaagggcctggatgcacccc              >  2:60538/1‑230 (MQ=255)                                                                                                                                                                                                                                aaTCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAgcg          >  1:349876/1‑249 (MQ=255)                                                                                                                                                                                                                                        aaaTCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGGCAGGTCATGATGCTCCGCCCGCGCcacgggg  >  1:228067/1‑247 (MQ=255)                                                                                                                                                                                                                                        aaaTCTCGCCGCGCCCCCGGTCCTGACGAGCAAGGGAAATCAATACGCCAGCAAGGGTCGCGCGATGGGGCTGAGTAGTCGCCAGCGATGCGCGAATCGCCGTTCGGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAGCGCGCTACCAGCCAGATGGCCGCCTTCACCGGGGTCTTGTGCCTCGTTGCGGTTAAAGCAGTACGGCAGGTCAAGgtggtggtggtcggggggggcggcgggg  <  2:158919/251‑23 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAG‑TTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCG  >  NC_000913/3815046‑3815526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑