Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA28.2% 54.3 / 11.7 39E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (24/4);  new base A (0/11);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 8.14e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATT  >  NC_000913/3177505‑3177993
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
cccccGGGTTTCCCGGGAAACTTAACCCCGGTTTGCCCCGTTTGCCTCTCATCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCGCGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                                                                                                                                <  1:232036/251‑1 (MQ=255)
  cccGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTc                                                                                                                                                                                                                                              >  2:289696/1‑251 (MQ=255)
                gAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAt                                                                                                                                                                                                                                >  1:308253/1‑251 (MQ=255)
                                             cTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGATCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                                                                                                                    >  1:131087/1‑250 (MQ=255)
                                                  aGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCAGCCGCACCTCATGCCTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                                                                                                                               >  1:413489/1‑250 (MQ=255)
                                                    ccccGCCTCTTCAACCGCTTCGGGACGGGCAACATCTTCCACCCTTTCCCCCTCTCCAATCACCCCGGCACCCATC‑CCCAGAAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCGCGCGCGGGGTAAAA‑GGGG‑AGCAAGACTGCGGGGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                                                                                                                            <  1:25048/250‑1 (MQ=255)
                                                        cccttttCAATCGCTCCGCGCCGGGCAACATCTTCCCCCCTTTCACCCTCTTCAATCCCCCCGTCACCCATC‑TCCAGTAGCCAGGGGGTTTCGCTGGTGTCTTCCGCGGCAATCCGATTCTGTCCAATCACCACACCTCCATCCCGCCCGGGGTCAAA‑GGGT‑AGCAAGCCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCAGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                                                                                                                        <  1:269867/246‑1 (MQ=255)
                                                         ccTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGCAATCtata                                                                                                                                                                                        >  2:378858/1‑250 (MQ=255)
                                                              tCAATCGCTCCGCGCCGGGCAACATCTTCCACACTTTCCCCCTCTTCAACCACCCCGG‑AACCATCCTCCAGAAGCCAAGGGTTTCCTCTGGTGTCGTACGCGCCAATCCGAATCTGCTCAATCAGCACAATTTCATCCCGCACGGGGTAAAA‑GGGT‑AGCAAGACTGCGGCGTCACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                                                                                                                                   <  1:180219/250‑1 (MQ=255)
                                                              ccaaccGCTCCGCGACGGGCAACATCTTCCACCCTTCCCCCCTCTACAATCCCCCCGGCACCCACC‑CCCAGTAGCAAGGGGGTTTCGCTGGGGTCGTACGCGGCAACCCGAATCTGCTAAATCAGCACAACTTCATCACGCACTGGGTAAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                                                                                                                                  <  1:346817/246‑1 (MQ=255)
                                                                aaTCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                                                                                                                                 >  1:330355/1‑250 (MQ=255)
                                                                  ccctttccccatgtgaaaaaTCTTCCACACTTTCCCCCTTTTCAATCACCCCGCCAACCATC‑TCAAATGGCCAGGGGGTTTCGCTGGTTTCGTACTCTGCAATCCGAATCTGCGCAAACAGCAAAACTCCACCCCTCACTGGTACAAA‑GGGA‑AGCAAGCCTGCGGCTTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                                                                                                                              <  2:164864/232‑1 (MQ=255)
                                                                   cGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAANAGACGATGACGAAATCTATAAAGATCTAATg                                                                                                                                                                             >  2:241597/1‑251 (MQ=255)
                                                                        cccgACGGGCAACCTCTTCCACACTTTCCCCCTCTTCAATCACCCCGCCACCCACC‑TCAGGTAGCCAAGGGGTTTCTCTGGTGCCGTACGCGGCAATCCGAATCTGCTCAATCAGCCCACCTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                                                                                                                        <  1:104747/249‑1 (MQ=255)
                                                                                        ttCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                                                                                                                        >  1:343664/1‑251 (MQ=255)
                                                                                         cccccattctcccccTCTACAATCACCCCGACAACCAAC‑CCAAGAAGCAAGGGGGTTTCGCAGGTGGCGAACGCGAAAACCCAAATCGGCACAATCAACAAAACTACAACACGCAC‑GGGTAAAAAGGGA‑AGCAAGACGGGGGCGTGCCCGCGCAAAAAAATTACCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                                                                                                                         <  2:330355/237‑1 (MQ=255)
                                                                                                  tcccccTCTTCAATCCCCCCGGAACCCATC‑CCCAGTAGCCAATGGTTTTCGCGGGTGCCGTACGCGCCAATCAGATTCTGCTCAACCACCACAACTCAATCACTCACTGGGTCAAA‑GGGT‑AGAAATACTGCGGCGGTACCGCTCTAAAAAATTCCCCGCCGCTCCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                                                                                                                               <  1:254999/247‑1 (MQ=255)
                                                                                                     cccTCTTCAATCACCCCGGCACCCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGACAAA‑GGGT‑AGCAAGACTGCGGCGGGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                                                                                                                           <  1:326692/251‑1 (MQ=255)
                                                                                                                   cccGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt                                                                                                                             >  1:84304/1‑251 (MQ=255)
                                                                                                                     cGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAANTGTTTCTCGTGCAATAATTTCTACATCGttt                                                                                                                           >  2:215325/1‑251 (MQ=255)
                                                                                                                        cAACCATC‑TCCAGTAGCCAGGGGGTTCCGCGGGGGCCGTCCGCGGAAACCAGAATCGGCAAAACCAGCACAACTTCCTCCCTCTCGGGGTAAAG‑GGGT‑AGCAAGACGGCGGCGTCACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                                                                                                                        <  1:190509/251‑1 (MQ=255)
                                                                                                                             aTC‑TCCAGTAGCCAAGAGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATAAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTACCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGAGCAATAATTTCTACATCGTTTTTGCCaa                                                                                                                    >  2:471196/1‑250 (MQ=255)
                                                                                                                               c‑tcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATg                                                                                                                 >  1:50856/1‑251 (MQ=255)
                                                                                                                                 tcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTAAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGGTTTTGCCAAATGt                                                                                                                >  1:295669/1‑251 (MQ=255)
                                                                                                                                      tAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg                                                                                                           >  1:83320/1‑251 (MQ=255)
                                                                                                                                        cccacgggtttcctgtgttggctgaCGCGGCAATCCGAATCTGCTCAACCAGCGCAATTTCATCACGCACTGGGT‑AAA‑GGGTTAGCAAGTCTGCGGCGCGACCCCTATAAAAATTTTCCCGCCGCACCGCATGACTCATTTGCCCGTTGAATAGACGATTACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc                                                                                                         <  2:134048/227‑1 (MQ=255)
                                                                                                                                                    cgcgggtttgttACGGGGCAATCCGAATCCGCAAAATCAGCAAAACTCCATCCCCAACTGGCTAAAG‑GGGT‑AGAAAAACGGGGGCGGGACCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGc                                                                                             <  1:333306/240‑1 (MQ=255)
                                                                                                                                                           gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTTTAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCAAAATGTAACGGGCAGGTTGTCTGGCTTAAGc                                                                                       >  2:341664/1‑250 (MQ=255)
                                                                                                                                                                          cctaaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTAACCGCGCTCCAAAATTTCCCGCCGCACCTCATGACACATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGCAAAAAAGCCGCGATAAAGTGTTTCTAGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTgg                                                                       >  2:356601/4‑251 (MQ=255)
                                                                                                                                                                          ccGAATCTGCTCAATCAGCACACCTCCACCACCCACGGGGTAAAA‑GGGT‑AGCAAGACTGCGGCTTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTgg                                                                       <  1:412206/251‑1 (MQ=255)
                                                                                                                                                                                       aTCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGa                                                           >  1:164198/1‑250 (MQ=255)
                                                                                                                                                                                           gCACAACTTCATCACGCACTGGGGAAAA‑GGGT‑AGAAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAACAAGCCGCGATACAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGAAACGGGCAGGTTGTCTGGCTTAAGCATTGTTATTGTCCTGGCACTAATAGTGAATTa                                                       >  2:389542/1‑250 (MQ=255)
                                                                                                                                                                                             acaACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                                                    >  1:62602/1‑251 (MQ=255)
                                                                                                                                                                                             acaACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                                                    >  1:480269/1‑251 (MQ=255)
                                                                                                                                                                                                aCTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGAATGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtgtg                                                 >  1:135858/1‑251 (MQ=255)
                                                                                                                                                                                                                 gTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCCCGCTCAAAACTTTCCCGCTGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTATTGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCTTTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGACTTTCAGCGACGTTTg                                >  1:264278/1‑251 (MQ=255)
                                                                                                                                                                                                                               cAAGACTGCGGCGAGACCGCGCTCAAAAATTTCCAGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATACTTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCCCTCATAGTGAATTAAATGTGAATTTAAGCGACGTTTGACTGCCGTTTg                     >  2:258284/1‑250 (MQ=255)
                                                                                                                                                                                                                                       cggcttccccgccaacAAACATTACCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAGAAAGTGTTTCGCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCAt            <  2:132765/236‑1 (MQ=255)
                                                                                                                                                                                                                                        ggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCNGCGACGTTTGACTGCCGTTTGAGCAGTCAtg           >  2:221361/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 ccgcTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAAtt  <  1:215325/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
CCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATC‑TCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATT  >  NC_000913/3177505‑3177993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑