Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,2340GT30.6% 46.8 / 11.5 36L25F (TTG→TTT) ycgYuncharacterized protein YcgY
Reads supporting (aligned to +/- strand):  ref base G (4/21);  new base T (11/0);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 2.27e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTA  >  NC_000913/1244993‑1245481
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                  
aGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCtt                                                                                                                                                                                                                                                           <  1:485546/251‑1 (MQ=255)
  ccGGATAGCGATACAGAAGCCGTAATCCGCTTTCACATCAGAACGTATACTCTACTCTTGCTTTACGGGTGAAAAAAATCAATATAGCCCTTCGATATTCGATAAAGGCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCTTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCTTTAAAAATGGGGCTGATAGTGAGGCGGTTTTGGCTTCGCTTAt                                                                                                                                                                                                                                                         <  2:31501/250‑1 (MQ=255)
    ggATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTGGCTgacgtttttt                                                                                                                                                                                                                                                       >  1:247571/1‑242 (MQ=255)
              aCAGATGCCGCTATCCGCTTTCACATCAGACCGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGa                                                                                                                                                                                                                                             <  2:377376/251‑1 (MQ=255)
                          aTCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGTA‑TTGA‑TCAATc                                                                                                                                                                                                                                <  1:367605/250‑1 (MQ=255)
                          aTCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATc                                                                                                                                                                                                                                <  2:60006/250‑1 (MQ=255)
                                    acaTCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTGGCCGCGCTTCTTGAACCttttggagttgtcaattctttttgccg                                                                                                                                                                                                                        >  1:80212/1‑226 (MQ=255)
                                             cGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATATCACGTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCATGCGTTAAAAATTGGGCTGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCaa                                                                                                                                                                                                         <  2:337017/251‑1 (MQ=255)
                                                ataCTCGACACCTTCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAAt                                                                                                                                                                                                      <  2:362021/251‑1 (MQ=255)
                                                ataCTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTCGGCGTATTGTACCATTAGAA‑TTGT‑TTAATACTTCtttccacttaagccaaaat                                                                                                                                                                                                         >  2:122916/1‑233 (MQ=255)
                                                                                                  atcagATAAAGGCGGTCCCCGGTAACAACATCAGAATCCTGTTTTAAGTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTGGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTGCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCtt                                                                                                                                                <  2:471278/247‑1 (MQ=255)
                                                                                                    ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAACAATGGGGCGGATAGTGAGGCGGTTTTTGGTGGGTTTATTGTAAATTTTGAA‑TTAAATCA‑TCCTTCTTGCTG‑ACTAT‑A‑CCAAAATA‑CCCGCTTGGCCATGCTTCATGTT‑CGTGACG‑AGGAATGGGCTGCA‑TTCtttt                                                                                                                                              >  2:172134/1‑251 (MQ=255)
                                                                                                            gTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTg                                                                                                                                      <  1:11010/251‑1 (MQ=255)
                                                                                                                       gTAACAACAACAGAATCCTTTTTTAATTATTNTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTGTGGTTGCTAAATTctct                                                                                                                           <  2:247571/251‑1 (MQ=255)
                                                                                                                             acaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAACATTTTGAA‑TTGA‑TCAATACTTCTTGGAG‑ATTAA‑G‑CCAAAAAAACCCGTT‑GGCAATGCTTTA‑GTTACGCGAAG‑AGGAATGGTTTGAA‑ATCTTTTTTTTGTTGCTAAATTCTCTGGAggg                                                                                                                     >  1:70742/1‑249 (MQ=255)
                                                                                                                              caacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTTCGCTTATTTAACATTTAGAA‑ATGA‑TCAATCCCTTTGGCAG‑ATTAC‑A‑CCAAAAAA‑ACCGTTCGGCCATGCTTCA‑GTTACGCGCAG‑AGGGATGGTCTGAA‑TTCTTTTTTTTGTTGCTAAATTTTCTGGAgggt                                                                                                                    >  2:348840/1‑248 (MQ=255)
                                                                                                                                      tCCTTTTTTAATTATTGTTTCTTGGTTGTTGTTGTGATTCGTAAAATAAATATTTTTGACAGGAGCCAGTCATGTGTTAAAAATGGGGCGGATATTTAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TTAATCCTTCTGGCAT‑AGTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGTAG‑AGGATTGGTCTGAA‑TGCTTTTTTTGTTTGCTAAATTCTCTGGAGTGTCAGGAtt                                                                                                             <  2:460093/250‑1 (MQ=255)
                                                                                                                                          tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtg                                                                                                          <  1:423272/249‑1 (MQ=255)
                                                                                                                                                attattGGTTCGTTGTTTTGGGTGTGATTCAGAAAAGAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGTGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATC‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATTGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGattatt                                                                                                 <  2:316633/251‑1 (MQ=255)
                                                                                                                                                  tattGGTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGTGCGTATTGGAAATTATGAA‑TTGG‑TCAATCCTTCTTGCAGGATTAA‑‑‑CCAAAAAA‑CCCCTTCGGCAATTCTTCT‑GTTACGAGACG‑AGGAATGGCCTGGA‑TTTTTTTTTTTGTTTCTTAATNTTCTGGAgggtaggggttttgtggttattt                                                                                                 >  2:230776/1‑228 (MQ=255)
                                                                                                                                                   attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTa‑a                                                                                              <  2:80212/250‑1 (MQ=255)
                                                                                                                                                   attGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTGGGTGGGCTTTTTGAAAATTTTGAA‑TTGA‑TCAAACCTTCTTGGAG‑GATAA‑A‑CCAAAAAA‑CCCGTTTGGCAAAGGTTTA‑GTTAAGCGAAGGAGGA‑TGGGCTGAAATTTTTTTTTTGGT‑GTTAAATTTTCTGGAggggttgggattttggtttttttat                                                                                               >  1:175742/1‑227 (MQ=255)
                                                                                                                                                    ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTATTTAACATTTTGAA‑TTGA‑TCAATCCTTCTTGGAG‑AATAA‑G‑CCCAAAAA‑ACCGGTCGGCCAAGCTTCC‑GATTCGCGAAG‑AGGGAAGGTCTGAA‑TTCTTTTTTTGGTTTCTTAATTTTCTTGGGGGGCTGGGTTTTG‑TGGTTATTA‑Att                                                                                            >  1:386167/1‑251 (MQ=255)
                                                                                                                                                                   tGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGATTATTTAAAAATTAGAA‑TTTA‑GCCAACCTTCTTGCAG‑GTTAA‑GGCCAAAA‑A‑CCCGTTCGGCCAAGCTTCA‑GGTACGCGGAG‑AGGGATTGGCTGGA‑ATCTTTTTTTGGTTGCTAAATTTTCTGGGGTGTCTGGGTTTTGGTG‑TTATTA‑ATTTAACTCCCGaaaac                                                                              >  1:140138/1‑248 (MQ=255)
                                                                                                                                                                      tgtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCCCTTATTGGACATTTTGAAATTG‑‑TCAAATCTTCTTGCCG‑ATTAAAC‑CCAAAAAC‑CCCCTTGGGAATTCCTTAA‑GTCA‑GCGAAA‑AGGAATGGTCTGAA‑TTTTTTTTTTTGTTGCTAAATTCTCTGGGGTGTCCGGATTTTG‑TGGTTATTT‑ATTTTAATCCAGAAAGCaaa                                                                           >  1:145123/1‑250 (MQ=255)
                                                                                                                                                                                     aaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGt                                                            <  1:263343/250‑1 (MQ=255)
                                                                                                                                                                                             tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTGAACATTTAGAA‑ATGA‑TCAATCCTTTTGGCAG‑ATTAA‑G‑CCAAAAAAACCCGT‑CGGCAATGGTTCC‑GTTACGCGGAA‑AGGGAAGGTCTGAA‑TTCTTTTTTTTGTTGCTAAATTCTCTTGAGGGTCTGGTTTTTG‑TGATTATTTTATTAAATTCC‑GAAAACAAAAAAAAGGTGATGAGTGAAAACCgc                                                   >  1:52897/1‑250 (MQ=255)
                                                                                                                                                                                                  aGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTGAAAATTTATAA‑TTGA‑TAAAACCTTCTGGGCG‑ATTAA‑A‑CCAAAAAA‑CACCTTCGGCAATGCTTCA‑GATACGCGCAG‑AGGGATAGTCTGAA‑TTCTTTTTTTGTGTGCTAAAATCTCTTGGGTGTCTGGGTTATG‑TGGTTTTTA‑ATTTATATCCCGAAAGACAAAAAACGTTGATGAGTGaaaaacgcaaac                                               >  2:348727/1‑240 (MQ=255)
                                                                                                                                                                                                      gCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATAtt                                          <  1:108300/251‑1 (MQ=255)
                                                                                                                                                                                                          gTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGCTTTTGCTGGGCTTTTTGAACATTTCGAA‑TTGG‑TCAATCCTTCTGGCCG‑GTTAA‑G‑CCCAAATA‑ACCGTTCGGCAAAGCTTCA‑GGTACGCGAAG‑AGGGAAGGTCTGGA‑TTCTTTTTTTTGGTGCTAAATTATCTGGAGAGTCTGGATTATT‑GGGTTTTTT‑ATTTAACTCCAGAAAGCAAAAAAAAGTTGATGAGTGAAAAACGTAACAATATTTAgg                                      >  1:183010/1‑251 (MQ=255)
                                                                                                                                                                                                            cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCGTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTTAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ACTTAACTCCAGAAAGCAAAAAAACGGTGATGAGTGAACACCGTAACAATATTCAGGt                                     <  1:166507/250‑1 (MQ=255)
                                                                                                                                                                                                                 gTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAAt                               <  1:172134/251‑1 (MQ=255)
                                                                                                                                                                                                                  ttAAAAAGGGGGCGGATAGTGATGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGTA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGATTGTCTGGATTATG‑TGATTATTA‑ATTTAACGCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCaa                                <  2:406974/249‑1 (MQ=255)
                                                                                                                                                                                                                          ggggCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                       <  1:348840/250‑1 (MQ=255)
                                                                                                                                                                                                                                       aGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGt         <  1:291482/251‑1 (MQ=255)
                                                                                                                                                                                                                                               ttGGCTGCGCTTATTGCAAATTTAGAA‑TAGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGACG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑CTTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATATACAACGTCGGCGTa  >  2:404065/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                  
AGGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAA‑TTGA‑TCAATCCTTCTGGCAG‑ATTAA‑G‑CCAAAATA‑CCCGTTCGGCAATGCTTCA‑GTTACGCGAAG‑AGGAATGGTCTGAA‑TTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATG‑TGATTATTA‑ATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTA  >  NC_000913/1244993‑1245481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑