Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,512,183	T→C	E54E (GAA→GAG)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,512,183	0	T	C	100.0%	305.0 / NA	81	E54E (GAA→GAG)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/30);  total (51/30)

CCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGC  >  NC_000913/3511944‑3512427                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     aattctggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGtttt                                                                                                                                                                                                                                           <  1:739023‑M2/69‑1 (MQ=255) aattctggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccggtacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGtttt                                                                                                                                                                                                                                           >  1:334085‑M2/183‑251 (MQ=255)    tctggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCa                                                                                                                                                                                                                                         >  1:680310‑M2/180‑250 (MQ=255)      tggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAAcg                                                                                                                                                                                                                                      >  1:377270‑M2/178‑251 (MQ=255)       ggccttcttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAAc                                                                                                                                                                                                                                       <  1:468928‑M2/73‑1 (MQ=255)          cttcttaatgcatctttttctatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGc                                                                                                                                                                                                                                  >  1:172941‑M2/174‑251 (MQ=255)              ttaatgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGc                                                                                                                                                                                                                              >  2:120716‑M2/170‑251 (MQ=255)                 atgactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTc                                                                                                                                                                                                                           >  1:746126‑M2/167‑251 (MQ=255)                   gactctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTc                                                                                                                                                                                                                           >  2:497197‑M2/165‑249 (MQ=255)                       ctttttatatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGcc                                                                                                                                                                                                                      >  1:637204‑M2/161‑250 (MQ=255)                             atatattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATATCTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGAt                                                                                                                                                                                                               <  2:213102‑M2/97‑1 (MQ=255)                               atattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATg                                                                                                                                                                                                              >  1:699803‑M2/153‑250 (MQ=255)                                tattcagcaaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGa                                                                                                                                                                                                             >  1:722645‑M2/152‑250 (MQ=255)                                       caaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCNGCCTGCGTCAGGCCAACGGATGAACGGcatt                                                                                                                                                                                                     >  2:378562‑M2/145‑251 (MQ=255)                                        aaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttccaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGTTGAACGGcattc                                                                                                                                                                                                    >  1:485341‑M2/144‑251 (MQ=255)                                        aaataaaacatagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGcattc                                                                                                                                                                                                    <  2:80298‑M2/108‑1 (MQ=255)                                                 atagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGcc                                                                                                                                                                                             >  1:33961‑M2/135‑249 (MQ=255)                                                  tagccccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGc                                                                                                                                                                                          >  1:315819‑M2/134‑251 (MQ=255)                                                  tagccccttaataaatattatctgctaatgggttatattcaccgttacaacgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGc                                                                                                                                                                                          >  1:428831‑M2/134‑251 (MQ=255)                                                      cccttaataaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCAt                                                                                                                                                                                      >  2:288048‑M2/130‑251 (MQ=255)                                                              aaatattatctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGtt                                                                                                                                                                              <  2:115356‑M2/130‑1 (MQ=255)                                                                      tctgctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCg                                                                                                                                                                      >  2:538260‑M2/114‑251 (MQ=255)                                                                          ctaatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGTCCAACGGATGAACGGCGTTCATNTGCCAGCCCATCTATGGTTTTCAATCGAtt                                                                                                                                                                   >  2:395052‑M2/110‑250 (MQ=255)                                                                            aatgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTAcc                                                                                                                                                                >  2:221514‑M2/108‑251 (MQ=255)                                                                             atgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatatccCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCATGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCg                                                                                                                                                               <  1:723224‑M2/145‑1 (MQ=255)                                                                              tgggttatattcaccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGGTTACCGc                                                                                                                                                              >  1:373542‑M2/106‑251 (MQ=255)                                                                                         caccgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCGGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAAGCGATTACCGCACGTTTCAccc                                                                                                                                                   <  2:543623‑M2/157‑1 (MQ=255)                                                                                            cgttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCcaac                                                                                                                                                >  2:8680‑M2/92‑251 (MQ=255)                                                                                              ttacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCcaaca                                                                                                                                               >  1:747754‑M2/90‑250 (MQ=255)                                                                                               tacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCcaacaa                                                                                                                                              >  2:739703‑M2/89‑250 (MQ=255)                                                                                               tacagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCcaacaa                                                                                                                                              >  1:89904‑M2/89‑250 (MQ=255)                                                                                                acagcgacttacaaaattaagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAAt                                                                                                                                            >  1:529559‑M2/88‑251 (MQ=255)                                                                                                            aaaattaagtcattcgctcactgacgccaattaaatattcctgtatagttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAAAGGGCCAGcg                                                                                                                                >  2:610446‑M2/76‑251 (MQ=255)                                                                                                                 taagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtg                                                                                                                           >  1:184816‑M2/71‑251 (MQ=255)                                                                                                                  aagtcattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtg                                                                                                                           >  2:536853‑M2/70‑250 (MQ=255)                                                                                                                      cattcgctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGTATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgac                                                                                                                      <  2:237119‑M2/186‑1 (MQ=255)                                                                                                                         tctctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCGCTTTTCACCCAACAAATAACGGGCCAGCTTCGTGATgacg                                                                                                                     <  2:746126‑M2/187‑1 (MQ=255)                                                                                                                           gctcactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGc                                                                                                                 >  1:470453‑M2/61‑251 (MQ=255)                                                                                                                              cactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCctt                                                                                                              >  1:293320‑M2/58‑251 (MQ=255)                                                                                                                              cactgacgccaattaaatattcctgtatcgttcttaacgagcagtcttcggatattcCTCGACCAGAGAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCctt                                                                                                              <  2:16096‑M2/194‑1 (MQ=255)                                                                                                                               ccttacgtcaattaaatatgcctgtatcgttcttaacgagcagtcttcgtattttcCCAGACCAGATACCTGCCATTGCGCCCACGGCGTTCAATCCGTCTCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttc                                                                                                             <  2:680310‑M2/195‑1 (MQ=255)                                                                                                                                  gacgccaattaaatattcctttatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttct                                                                                                            <  2:637204‑M2/196‑1 (MQ=255)                                                                                                                                  gacgccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCcttct                                                                                                            <  1:536853‑M2/196‑1 (MQ=255)                                                                                                                                     gccaattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCACGACCAGATAACTGCCATTGCGCCCACGGGGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCtttttt                                                                                                       <  1:673359‑M2/201‑1 (MQ=255)                                                                                                                                        aattaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGAt                                                                                                    >  1:277499‑M2/48‑251 (MQ=255)                                                                                                                                          ttaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCAc                                                                                                                                                     <  2:729263‑M2/155‑1 (MQ=255)                                                                                                                                          ttaaatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCAc                                                                                                                                                     >  1:729263‑M2/46‑200 (MQ=255)                                                                                                                                             aatattcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACg                                                                                               >  1:581144‑M2/43‑251 (MQ=255)                                                                                                                                                 ttcctgtatcgttcttaacgagcagtcttcgtatattcCACGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTAGCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTc                                                                                            <  2:89904‑M2/212‑1 (MQ=255)                                                                                                                                                  tcctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCg                                                                                           >  2:94900‑M2/38‑250 (MQ=255)                                                                                                                                                   cctgtatcgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAc                                                                                         <  1:252752‑M2/215‑1 (MQ=255)                                                                                                                                                          cgttcttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCataat                                                                                  >  2:764790‑M2/30‑251 (MQ=255)                                                                                                                                                           gttcttaacgagcagtcttcgtatcttcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCACCGGCTGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGTCTACCCCTTCTTTTTTGCTAAACGTTCGCCgcatact                                                                                  >  2:167365‑M2/29‑248 (MQ=255)                                                                                                                                                               ttaacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCATGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATCAAtt                                                                             >  2:20826‑M2/25‑251 (MQ=255)                                                                                                                                                                taacgagcagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAAttt                                                                            >  1:407575‑M2/24‑251 (MQ=255)                                                                                                                                                                       cagtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTa                                                                      >  2:397013‑M2/17‑250 (MQ=255)                                                                                                                                                                         gtcttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAt                                                                   <  1:221514‑M2/237‑1 (MQ=255)                                                                                                                                                                            ttcgtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTg                                                                 >  1:345804‑M2/12‑250 (MQ=255)                                                                                                                                                                            ttcggatattcCTCGACCAGATAACTGCCATTGAGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATCTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACACAACAAATAACGGGCCCGCGTCGTGATGTCGACGCCTTCTTTTTTGATAAACGTTCGCCGCATAATAAATTTTCAATAAATTg                                                                 >  2:15362‑M2/12‑250 (MQ=255)                                                                                                                                                                               gtatattcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCCCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATATTAAATTTTCAGTAAATTGaatt                                                             >  2:301654‑M2/9‑250 (MQ=255)                                                                                                                                                                                     tcCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat                                                        <  1:497197‑M2/248‑1 (MQ=255)                                                                                                                                                                                      cCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTatat                                                      >  2:465692‑M2/2‑251 (MQ=255)                                                                                                                                                                                       ctcGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATc                                                     >  1:192295/1‑251 (MQ=255)                                                                                                                                                                                         cGACCAGATAACGGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt                                                   <  2:529559/251‑1 (MQ=255)                                                                                                                                                                                             cAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacga                                                                                                                    >  2:512622/1‑182 (MQ=255)                                                                                                                                                                                             cAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacga                                                                                                                    <  1:512622/182‑1 (MQ=255)                                                                                                                                                                                                   aCTGCCATTGCGCCCCCGGCGTGCAATCCGTATCGCTCCGATTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCTCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTATTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTg                                         <  2:479944/251‑1 (MQ=255)                                                                                                                                                                                                     tGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGa                                        >  1:507345/1‑250 (MQ=255)                                                                                                                                                                                                        cATTGCGCCCACGGCTTTTAATCCGTATCGCTCCGCTTGACTCTATCGTTTTCAACGCGGCCTGCGTCAGGCCATCGGATGATCGGCATTCATTTGCCAGCTCAGCTATGGTTTTCGATCGATTACCTCACTTGTCACCCATCAAATAACGGGCCAGCGTCGTGATGACGTCGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGGATTTGAAGAt                                    <  2:736415/251‑1 (MQ=255)                                                                                                                                                                                                         aTTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCttttt                                                                                                        <  2:570235/182‑1 (MQ=255)                                                                                                                                                                                                         aTTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCttttt                                                                                                        >  1:570235/1‑182 (MQ=255)                                                                                                                                                                                                         aTTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGAt                                    >  1:316011/1‑250 (MQ=255)                                                                                                                                                                                                                            aTCCGTATCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtgt                >  1:615576/1‑251 (MQ=255)                                                                                                                                                                                                                               cggaTCGCTCCGCTTGACTCCAGCGGTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACTACGGCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTGCAGGAATGTGAAGATAAGGCGCAACAGCGAGATGTGGa             <  2:74659/248‑1 (MQ=255)                                                                                                                                                                                                                                  aTCGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAAc          <  2:581144/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACgg        <  1:739703/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGc       <  1:395052/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCTCCGCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGc       <  2:407575/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccGCTTGACTCCAGCGTTTTCAACGCGGCCTTCGTCAGGCCAACGGATGTACGGCATTCATTTGCCAGCGCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTTAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAg    <  2:624660/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCCACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTAGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGc  >  1:593855/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCTTGACTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGc  >  1:5251/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTGCACATACCACGTTGTTGATATCCACATGAGTCAGCAATGCTTTGTTATCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGC  >  NC_000913/3511944‑3512427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑