Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,499,391	G→T	intergenic (+190/‑57)	cysG → / → yhfL	siroheme synthase/DUF4223 domain‑containing lipoprotein YhfL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,391	0	G	T	100.0%	248.7 / NA	69	intergenic (+190/‑57)	cysG/yhfL	siroheme synthase/DUF4223 domain‑containing lipoprotein YhfL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/22);  total (47/22)

ATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGC  >  NC_000913/3499148‑3499634                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    attGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTatat                                                                                                                                                                                                                                              >  1:210725/1‑251 (MQ=255)     gTCGGGTTGTTGGCCTGCGCGATAAACTGAANTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCct                                                                                                                                                                                                                                          <  2:339596‑M1/251‑3 (MQ=255)       cGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcg                                                                                                                                                                                                                                        >  1:736415‑M1/1‑247 (MQ=255)                                 gaactgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacg                                                                                                                                                                                                              <  2:239526‑M1/251‑31 (MQ=255)                                 gaactgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacg                                                                                                                                                                                                              >  2:252752‑M1/1‑221 (MQ=255)                                    ctgGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcg                                                                                                                                                                                                           >  1:115356‑M1/1‑218 (MQ=255)                                       gTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttc                                                                                                                                                                                                        >  1:164119‑M1/1‑215 (MQ=255)                                       gTTCTCCAACCATTAATTGAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttc                                                                                                                                                                                                        <  2:159367‑M1/251‑37 (MQ=255)                                            ccAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTCTATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatcc                                                                                                                                                                                                   >  1:74659‑M1/1‑210 (MQ=255)                                                 cATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatc                                                                                                                                                                                              <  2:315857‑M1/251‑47 (MQ=255)                                                      aTTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctcc                                                                                                                                                                                         >  1:630465‑M1/1‑200 (MQ=255)                                                                        aaTTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgtttt                                                                                                                                                                       <  1:739023‑M1/251‑70 (MQ=255)                                                                        aaTTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGGTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgtttt                                                                                                                                                                       >  1:334085‑M1/1‑182 (MQ=255)                                                                           tCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttca                                                                                                                                                                     >  1:680310‑M1/1‑179 (MQ=255)                                                                             tGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacg                                                                                                                                                                  >  1:377270‑M1/1‑177 (MQ=255)                                                                              ggCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaac                                                                                                                                                                   <  1:468928‑M1/249‑74 (MQ=255)                                                                                 cttcttAATGCATCTTTTTCTATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggc                                                                                                                                                              >  1:172941‑M1/1‑173 (MQ=255)                                                                                     ttAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATAtt                                                                                                                                                                                                                                             >  1:602116/1‑168 (MQ=255)                                                                                     ttAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATAtt                                                                                                                                                                                                                                             <  2:602116/168‑1 (MQ=255)                                                                                     ttAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgc                                                                                                                                                          >  2:120716‑M1/1‑169 (MQ=255)                                                                                        aTGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtc                                                                                                                                                       >  1:746126‑M1/1‑166 (MQ=255)                                                                                          gACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtc                                                                                                                                                       >  2:497197‑M1/1‑164 (MQ=255)                                                                                              ctTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggcc                                                                                                                                                  >  1:637204‑M1/1‑160 (MQ=255)                                                                                                    atatatTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagatatctgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggat                                                                                                                                           <  2:213102‑M1/251‑98 (MQ=255)                                                                                                      atatTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatg                                                                                                                                          >  1:699803‑M1/1‑152 (MQ=255)                                                                                                       tatTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatga                                                                                                                                         >  1:722645‑M1/1‑151 (MQ=255)                                                                                                              cAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcngcctgcgtcaggccaacggatgaacggcatt                                                                                                                                 >  2:378562‑M1/1‑144 (MQ=255)                                                                                                               aaaTAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTCCAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggttgaacggcattc                                                                                                                                >  1:485341‑M1/1‑143 (MQ=255)                                                                                                               aaaTAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattc                                                                                                                                <  2:80298‑M1/251‑109 (MQ=255)                                                                                                                        aTAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgcc                                                                                                                         >  1:33961‑M1/1‑134 (MQ=255)                                                                                                                         tAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagc                                                                                                                      >  1:315819‑M1/1‑133 (MQ=255)                                                                                                                         tAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAACGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagc                                                                                                                      >  1:428831‑M1/1‑133 (MQ=255)                                                                                                                             cccTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcat                                                                                                                  >  2:288048‑M1/1‑129 (MQ=255)                                                                                                                                     aaataTTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggtt                                                                                                          <  2:115356‑M1/251‑131 (MQ=255)                                                                                                                                             tCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcg                                                                                                  >  2:538260‑M1/1‑113 (MQ=255)                                                                                                                                                 cTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcagtccaacggatgaacggcgttcatntgccagcccatctatggttttcaatcgatt                                                                                               >  2:395052‑M1/1‑109 (MQ=255)                                                                                                                                                   aaTGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattacc                                                                                            >  2:221514‑M1/1‑107 (MQ=255)                                                                                                                                                    aTGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATCCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcatgccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccg                                                                                           <  1:723224‑M1/251‑146 (MQ=255)                                                                                                                                                     tGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcggttaccgc                                                                                          >  1:373542‑M1/1‑105 (MQ=255)                                                                                                                                                                cACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccggcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaagcgattaccgcacgtttcaccc                                                                               <  2:543623‑M1/251‑158 (MQ=255)                                                                                                                                                                   cGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaac                                                                            >  2:8680‑M1/1‑91 (MQ=255)                                                                                                                                                                     ttACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaaca                                                                           >  1:747754‑M1/1‑89 (MQ=255)                                                                                                                                                                      tACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaa                                                                          >  2:739703‑M1/1‑88 (MQ=255)                                                                                                                                                                      tACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaa                                                                          >  1:89904‑M1/1‑88 (MQ=255)                                                                                                                                                                       aCAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaat                                                                        >  1:529559‑M1/1‑87 (MQ=255)                                                                                                                                                                                   aaaaTTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATAGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataaagggccagcg                                                            >  2:610446‑M1/1‑75 (MQ=255)                                                                                                                                                                                        tAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtg                                                       >  1:184816‑M1/1‑70 (MQ=255)                                                                                                                                                                                         aaGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtg                                                       >  2:536853‑M1/1‑69 (MQ=255)                                                                                                                                                                                             cATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggtattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgac                                                  <  2:237119‑M1/251‑187 (MQ=255)                                                                                                                                                                                                tctctcaCTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcgcttttcacccaacaaataacgggccagcttcgtgatgacg                                                 <  2:746126‑M1/246‑188 (MQ=255)                                                                                                                                                                                                  gCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgc                                             >  1:470453‑M1/1‑60 (MQ=255)                                                                                                                                                                                                     cACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgcctt                                          >  1:293320‑M1/1‑57 (MQ=255)                                                                                                                                                                                                     cACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGGATATTCctcgaccagagaactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgcctt                                          <  2:16096‑M1/251‑195 (MQ=255)                                                                                                                                                                                                      ccttacgtcaattaaatATGCCTGTATCGTTCTTAACGAGCAGTCTTCGTATTTTCccagaccagatacctgccattgcgcccacggcgttcaatccgtctcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttc                                         <  2:680310‑M1/243‑196 (MQ=255)                                                                                                                                                                                                         gACGCCAATTAAATATTCCTTTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttct                                        <  2:637204‑M1/249‑197 (MQ=255)                                                                                                                                                                                                         gACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttct                                        <  1:536853‑M1/249‑197 (MQ=255)                                                                                                                                                                                                            gCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCcacgaccagataactgccattgcgcccacggggttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttctttttt                                   <  1:673359‑M1/251‑202 (MQ=255)                                                                                                                                                                                                               aaTTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgat                                >  1:277499‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                 ttAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcac                                                                                 >  1:729263‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                 ttAAATATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcac                                                                                 <  2:729263‑M1/200‑156 (MQ=255)                                                                                                                                                                                                                    aaTATTCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacg                           >  1:581144‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                        ttCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCcacgaccagataactgccattgcgcccacggcgttcaatccgtagcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttc                        <  2:89904‑M1/250‑213 (MQ=255)                                                                                                                                                                                                                         tCCTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcg                       >  2:94900‑M1/1‑37 (MQ=255)                                                                                                                                                                                                                          ccTGTATCGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgac                     <  1:252752‑M1/251‑216 (MQ=255)                                                                                                                                                                                                                                 cGTTCTTAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgacgcataat              >  2:764790‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                  gTTCTTAACGAGCAGTCTTCGTATCTTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaccggctgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgtctaccccttcttttttgctaaacgttcgccgcatact              >  2:167365‑M1/1‑28 (MQ=255)                                                                                                                                                                                                                                      ttAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcatgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgacgcataatcaatt         >  2:20826‑M1/1‑24 (MQ=255)                                                                                                                                                                                                                                       tAACGAGCAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgacgcataataaattt        >  1:407575‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                                              cAGTCTTCGTATATTCctcgaccagataactgccattgcgcccacggcgttcaatccgtatcgctccgcttgactccagcgttttcaacgcggcctgcgtcaggccaacggatgaacggcattcatttgccagctcatctatggttttcaatcgattaccgcacttttcacccaacaaataacgggccagcgtcgtgatgacgacgccttcttttttgataaacgttcgacgcataataaattttcagta  >  2:397013‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCGGGCCAGAGAATTCTGGCCTTCTTAATGACTCTTTTTATATATTCAGCAAATAAAACATAGCCCCTTAATAAATATTATCTGCTAATGGGTTATATTCACCGTTACAGCGACTTACAAAATTAAGTCATTCGCTCACTGACGCCAATTAAATATTCCTGTATCGTTCTTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGC  >  NC_000913/3499148‑3499634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑