BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	98.0%	373.6 / NA	102	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (58/42); minor base G (0/2); total (58/44)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCA  >  NC_000913/3485961‑3486442

aGTAATACATTGATGTACTGCATGTATGCCAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                                >  1:240673/1‑251 (MQ=255)

aGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                                >  1:509603/1‑251 (MQ=255)

aGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                                >  1:651686/1‑251 (MQ=255)

 gTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAtt                                                                                                                                                                                                                                               >  2:200594/1‑251 (MQ=255)

     tACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCAcc                                                                                                                                                                                                                                           >  1:458977/1‑251 (MQ=255)

        aTTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAgg                                                                                                                                                                                                                                        >  1:80352/1‑251 (MQ=255)

          ggaggTACTGCAGGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGt                                                                                                                                                                                                                                      <  1:365899/247‑1 (MQ=255)

            aTGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGCAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACNCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                     >  2:394648/1‑250 (MQ=255)

            aTGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCACTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTATTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                     >  1:33699/1‑250 (MQ=255)

                  tGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGc                                                                                                                                                                                                                              >  2:89748/1‑251 (MQ=255)

                   gCATGTATGCAAAGGACGTCACATTACCGTGCAGTACCGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCg                                                                                                                                                                                                                             >  1:626697/1‑251 (MQ=255)

                   gCATGTATGAAAAGGACGTCCCATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGGAGCGGGACGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGGGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAg                                                                                                                                                                                                                               <  2:553921/249‑1 (MQ=255)

                     aTGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGCGAAAAAGCgg                                                                                                                                                                                                                            >  1:361342/1‑250 (MQ=255)

                       gTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGNAAGCTTATTCACCAGGGGGAAAAAGCGGaa                                                                                                                                                                                                                          >  2:354724/1‑250 (MQ=255)

                         aTGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTGGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                                                                                                                                        <  2:189695/250‑1 (MQ=255)

                         aTGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTCCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACg                                                                                                                                                                                                                       <  1:394648/251‑1 (MQ=255)

                             aaaGGCCGTCACATTACCGTGCATGACAGTTGATAGCCCCTCCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGTCTCTGGAAAAAGCTTATAACAGAG‑GATAACCGCGCATGTTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGaaa                                                                                                                                                                                                                         <  2:167736/245‑1 (MQ=255)

                             aaaGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGaaa                                                                                                                                                                                                                         >  1:167736/1‑245 (MQ=255)

                              aaGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGAACCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                                                                                                   >  2:633238/1‑250 (MQ=255)

                                      cacaTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCGGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                                                                                                                                                                                                           <  1:398782/250‑1 (MQ=255)

                                      cacaTTACCGTGCAGTACAGTTGAGAGCCCCTCCCCAGGTAGCGGGAAGCATATTCCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                                                                                                                                          <  2:240673/251‑1 (MQ=255)

                                      cacaTTACCGGCCAGTACAGTCGATACCCCCTCCCCAGGTAGCGGGAAGTATATTTCGTCAATCCAGAGCCAGCGCCTTTTTCTGCCCCTGGATAAAGCTTATACCATAG‑GATAACCGCCCATGGTGCTTGGCACACGGCAAACAGGCCCTACTCGCGAATGGTTCGTGTCTCAGTGCCACATTCATAAGGACCCATCCACGAGCAAGCTTATTCGCCAGGTTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                                                                                                                                          <  2:31742/251‑1 (MQ=255)

                                       ccaTTCCCGTGCAGTACGTTTGAAAGCCCCTTCCCAGGTAGCGGGAGGCTTATTCCGGCAAACCAGAGACGGCGGCGTTTGCTGGCTTTGGAGAAAGCTTATACCAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATGCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                                                                                                                                                                                                         <  2:546441/250‑1 (MQ=255)

                                        caTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                                                                                                                                        >  1:565209/1‑251 (MQ=255)

                                        caTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGATTCTTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                                                                                                                                        >  2:165069/1‑251 (MQ=255)

                                         atgcccGTGCAGTACAGTTAATACCCCCTTCCCAGGTAGCTGGAAGCATATGCCGGCAATCCAGAGACTGCGGCGTTATCTGGCCCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGATCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTGCTACATCGtga                                                                                                                                                                                                       <  2:131702/247‑3 (MQ=255)

                                           tACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                                                                                                                                      >  2:614852/1‑250 (MQ=255)

                                               gTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGcc                                                                                                                                                                                                >  2:663951/1‑250 (MQ=255)

                                               gTGCAGTACAGTTGATAGCCACTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATAGTTAAA‑GGct                                                                                                                                                                                                >  1:11873/1‑251 (MQ=255)

                                                 gCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGctct                                                                                                                                                                                              >  1:705215/1‑251 (MQ=255)

                                                 gCAGTACAGTTGAGAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGctct                                                                                                                                                                                              <  2:289492/251‑1 (MQ=255)

                                                        aGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCAACAGGGTGAAACAACGGAAACGCTGTACTACATCGTTAAA‑GGCTCTTTGG‑c                                                                                                                                                                                        >  2:728623/1‑249 (MQ=255)

                                                           tGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAACGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑c                                                                                                                                                                                  >  1:40448/1‑251 (MQ=255)

                                                            gATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑Ct                                                                                                                                                                                 <  1:85250/251‑1 (MQ=255)

                                                            gATAGCCCCTTCCCAGGTAGCGGGAAGCATATTCCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑Ct                                                                                                                                                                                 <  1:354724/251‑1 (MQ=255)

                                                                gCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAGAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATc                                                                                                                                                                             <  1:199572/251‑1 (MQ=255)

                                                                  cccTTCCCCGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACCGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTAATTCACCAGGGAGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCa                                                                                                                                                                            >  1:139292/1‑250 (MQ=255)

                                                                   ccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTCTCTGGCTCTGGAGAAAGCTTATAACAGAG‑GCTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATACGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCaa                                                                                                                                                                           >  2:174652/1‑250 (MQ=255)

                                                                      tCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAg                                                                                                                                                                         <  1:308148/249‑1 (MQ=255)

                                                                         cAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGaa                                                                                                                                                                    <  1:368606/251‑1 (MQ=255)

                                                                          aGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGa                                                                                                                                                                     >  1:309011/1‑249 (MQ=255)

                                                                            gTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAag                                                                                                                                                                   >  1:245751/1‑249 (MQ=255)

                                                                               gCGGGGAGCATATTTCGGCAATCCGGATGCAGGGTCTTTATCTGGTTCTGGAAAAAGCTTATACCAGAG‑GATAGCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTGCATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGt                                                                                                                                                              <  2:509603/251‑1 (MQ=255)

                                                                                   gcaGCATATTTCGGAAATCCAGAGCCAGAGCCGTTATCTGGCTCCGGAGATAGCCTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACAGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGCACCCATCCAAGAGCAAGCTTATTCACCCAGGTGAAAAAGCGGCAACGATGTACTACATCGTTAAA‑GTCTCTGTGG‑CAGTG‑CTAACCAAAGACGTAGAgggaaacg                                                                                                                                                          >  2:720069/3‑246 (MQ=255)

                                                                                   gAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTaa                                                                                                                                                            >  2:316407/1‑249 (MQ=255)

                                                                                      gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGaaa                                                                                                                                                       <  2:119423/251‑1 (MQ=255)

                                                                                          attacGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGTTTATAACAGAG‑GATAACCGCGCAGGGTGCTTGGCAAACCGAAACCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGAt                                                                                                                                                  <  2:245751/247‑1 (MQ=255)

                                                                                              cGGCAATCCAGAGACAGGGGCGTTATCTGGNCCGGGAGAATGCTAATAACAGAG‑GAACACCGCGCATGGTGCTTGGCAACCCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCGTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCct                                                                                                                                               <  2:361342/250‑1 (MQ=255)

                                                                                                 cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTc‑c                                                                                                                                          >  1:95161/1‑251 (MQ=255)

                                                                                                 cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGAGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTc‑c                                                                                                                                          >  1:77161/1‑251 (MQ=255)

                                                                                                  aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑Ct                                                                                                                                         >  1:458317/1‑251 (MQ=255)

                                                                                                  aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑Ct                                                                                                                                         >  1:373415/1‑251 (MQ=255)

                                                                                                   atcagtagccagcgccgttttccacctctGGAGAAAGCTTAGAACAGAG‑GATACCCTCGCAGGGTTCTGGGCAAACCCCAAACAGACCCTACTCTCGAAAGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCTGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTT‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTa                                                                                                                                        <  2:276335/226‑1 (MQ=255)

                                                                                                   aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGACAGCTTATAAAAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGATTATTCACCAGGGTTAAAAAGAGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGAG‑ATGATCAAAGTCGACGAGGGTAAAGAAA‑TGATCCTCTC‑Ctt                                                                                                                                        >  2:601854/1‑250 (MQ=255)

                                                                                                   aTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGAG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑Ct                                                                                                                                         >  1:48825/1‑250 (MQ=255)

                                                                                                     ccAGAGCCAGCGGCGTTATCTGGCTCTGGAAAAATCTGATAACA‑AGAGATAACCGGTCGTGGTGCTTGGCAAACCGCAAACAGTCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACAGTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTTAAAAAGCGGAAACGCTGTACTACAGCGTTAAA‑GTCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATc                                                                                                                                      <  2:290043/251‑1 (MQ=255)

                                                                                                      caggagcagcgactttATCTGGCTCTGGAGAAAGCTTTAAACAGAG‑GATAACAGCGCATGGTGCTTGGAAAGCCGCAAAACGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCGTATTCACCAGGGTGAAAAATCGGAAACGCTGTAATACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGCTCCTCTC‑CTATCt                                                                                                                                     <  2:101771/237‑1 (MQ=255)

                                                                                                       agagACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTg                                                                                                                                    >  1:302022/1‑251 (MQ=255)

                                                                                                          gaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAAt                                                                                                                                 >  2:21966/1‑251 (MQ=255)

                                                                                                            aaGCGGCGTTAGCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCa                                                                                                                               <  1:89748/250‑1 (MQ=255)

                                                                                                                ggcgTTATCTGGTTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGt                                                                                                                           <  2:651686/251‑1 (MQ=255)

                                                                                                                   gTTATCTGGCTCTGGAGAAAGCTTATAAGAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGa                                                                                                                                                                                                                                    >  1:56355/1‑148 (MQ=255)

                                                                                                                   gTTATCTGGCTCTGGAAAAAGCTAATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCCCCATGGTGa                                                                                                                                                                                                                                    <  2:56355/148‑1 (MQ=255)

                                                                                                                          ggCTCGGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGAtttt                                                                                                                     <  1:200594/247‑1 (MQ=255)

                                                                                                                           gccctGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAAAAGACCAGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTAACCAGGGTGAAAAAGCGGAAACGCTATACTACATCGTTAAC‑GGCTCTGTGG‑CAGTG‑CTTATCAACGACGAAGGGGGTCAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGATGATTTTATTgg                                                                                                                >  2:555873/4‑251 (MQ=255)

                                                                                                                             tatGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAAAGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATAAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTCTCTGAATCAGGGTGATTTTATTGGCg                                                                                                              >  2:644323/3‑251 (MQ=255)

                                                                                                                                ggAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGTTTATTCACCAGGGTGAAAACGCGGAAACGCTGTACTACAACGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCCGGGCGATTTTATTGGCGaa                                                                                                            >  2:560623/1‑250 (MQ=255)

                                                                                                                                   gaAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                                                                                                        <  1:374780/251‑1 (MQ=255)

                                                                                                                                          tataACAGAG‑GATAACCGCGCATGGTGNTTGGCAACCCACAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATATGAATCAGGGTGATTTTATTGGCGAACTGGGca                                                                                                     <  2:347578/247‑2 (MQ=255)

                                                                                                                                           ataACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGttt                                                                                                <  2:80352/251‑1 (MQ=255)

                                                                                                                                                 gag‑GATAACCGCGCTTGTTTCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CGGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAagag                                                                                          <  2:11873/251‑1 (MQ=255)

                                                                                                                                                  ag‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAgg                                                                                         >  1:421293/1‑251 (MQ=255)

                                                                                                                                                   g‑gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGGGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAggg                                                                                        >  1:419764/1‑251 (MQ=255)

                                                                                                                                                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAC‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGGTTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGcc                                                                                      >  2:311897/1‑249 (MQ=255)

                                                                                                                                                        aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg                                                                                    <  1:614852/251‑1 (MQ=255)

                                                                                                                                                           cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGAGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑a                                                                                 >  2:306838/1‑250 (MQ=255)

                                                                                                                                                            gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AAc                                                                               <  1:663951/251‑1 (MQ=255)

                                                                                                                                                                aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGt                                                                             >  1:498507/1‑249 (MQ=255)

                                                                                                                                                                   gTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAgcg                                                                         >  1:128610/1‑250 (MQ=255)

                                                                                                                                                                    ggctgggaaaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGGG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGAGCa                                                                       <  2:626697/243‑1 (MQ=255)

                                                                                                                                                                     ccttagCAAACCGCAACAAGTCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGTGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGTGTTATTTTATTGGCGAACTGGGCCGGTTTGAAGATGGCCAGG‑AACGTAGCGCAt                                                                      <  2:686533/246‑1 (MQ=255)

                                                                                                                                                                      cTTGGCACACCGGAAATAGACCCGTCTCTCGAATGGTTCTTGTTTAATTGCCACATTCATAAGTACCTATCAAAAAACAAGCTTATTCACAAGGGTGAAAAAGCGGAAACGCTGTACTACATAGTTAAA‑GGATCTGTGG‑CAGTG‑CTGATCAAAGACGACAAGGGTAAAGAAA‑TGATCCTCAC‑CTAGCTGAATCAGGGTGATTTTATTGGCAAACAGGACCTTGTTGAAGAGGGACTGG‑AACGTTGCGCATg                                                                     >  2:145032/1‑251 (MQ=255)

                                                                                                                                                                        tGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAAGCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATg                                                                     <  1:633238/249‑1 (MQ=255)

                                                                                                                                                                        gggaaaaCCGCAAACAAACCCGACTCTCGAANGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATggg                                                                   <  2:348474/247‑1 (MQ=255)

                                                                                                                                                                         ggCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATggg                                                                   <  2:95161/250‑1 (MQ=255)

                                                                                                                                                                          gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGt                                                                  >  1:722386/1‑250 (MQ=255)

                                                                                                                                                                            aacccGCAAACAGAGCCGACTCTCGAATGGTTCTTGTCTCAGTGCCACATTCATAAGTACCCAGCCAAGAGCAAGCTTATTCCCCAGGGTGAAAAAGTGGAAACGCAGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CGGATCAAAGACGAAGAGGGTAAAGAAA‑TTATCCTCTC‑CTATCTGAATCAGCGGGATGTTATTGGCGAACTGGGCCTTTTTGAATAGGGCCAGG‑AACGTAGCGCATGGGT‑ACg                                                              <  1:641654/248‑1 (MQ=255)

                                                                                                                                                                                          cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGGGCGAAAACCGCCt                                                <  2:309011/251‑1 (MQ=255)

                                                                                                                                                                                                 ctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCCGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAgg                                         >  2:79510/1‑250 (MQ=255)

                                                                                                                                                                                                    gAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGc                                      >  2:493257/1‑251 (MQ=255)

                                                                                                                                                                                                         gTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGACTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAACACCGCCTGTGAAGTGGCTGaa                                  >  1:365/1‑250 (MQ=255)

                                                                                                                                                                                                                   cATTGCCACATTCATAAGTCCCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACa                        >  2:406429/1‑250 (MQ=255)

                                                                                                                                                                                                                        ccACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAt                  >  1:370984/1‑251 (MQ=255)

                                                                                                                                                                                                                            aTTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCg              >  1:643771/1‑251 (MQ=255)

                                                                                                                                                                                                                             ttCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGc             >  1:304213/1‑251 (MQ=255)

                                                                                                                                                                                                                                aTAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCa           >  1:285675/1‑250 (MQ=255)

                                                                                                                                                                                                                                  aaGTACCCACACAAGAGCAGGCTTATCCCCCGGGGGGAAAAAGCGGAAACGCGGTACTACA‑CTTTAAAAGGCTCTGTGC‑AAGTC‑TTGATAAAAGACGAAGGGGGAAAAGAAA‑TG‑TCCCTTCTCAACCGGAATCAGGGTGTTTTTATTGGAGAACGGGGCCTGTTTAAAG‑GGGCCAGGGAACGTGGCTC‑TGGGTTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt        <  2:302022/251‑1 (MQ=255)

                                                                                                                                                                                                                                   agaccccATCCAAGAGAAGGCTTCTCCACCAGGGTAAAAAGGCGGAACCGCTGTTCTACATCTTTAAA‑GGCT‑TGGGG‑GAATGGCTGATAAAAGACGAAG‑GGGTAAAAAAAAGGACCCTCTC‑TTTCCGGAACCAGGGTGATTTTATTGGCGATCGGGGCTTGTTTGAAGGGGGCCAGG‑AACTTAGCGCAGGGGT‑AGGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg       <  1:306838/247‑1 (MQ=255)

                                                                                                                                                                                                                                   aGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg       >  1:427748/1‑251 (MQ=255)

                                                                                                                                                                                                                                        ccATCAAAGAGCAAGCTTTTTCACCGGGGTAAAAAAGAGAAAACGCTGTTATCCATC‑TTAAA‑GGGTTTGTGGGCCTGG‑CTGATAAAGGGCGAAGAGGTTAAAGAAT‑TGCTCCTCTC‑CTTACTGAATCGGGGTGTTTTTATTGGCGAACTGGGCTTTTTTGAAGGGGGGCAGG‑ACCGTAGCGC‑TGGGTTACGGGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCa  <  2:48825/251‑1 (MQ=255)

AGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAG‑GATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGG‑CAGTG‑CTGATCAAAGACGAAGAGGGTAAAGAAA‑TGATCCTCTC‑CTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGG‑AACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCA  >  NC_000913/3485961‑3486442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`