BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,310	C→T	A110V (GCG→GTG)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,310	0	C	T	97.5%	410.5 / NA	123	A110V (GCG→GTG)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (51/68); minor base G (3/0); total (55/68)
Fisher's exact test for biased strand distribution p-value = 8.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

ATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAG  >  NC_000913/2790066‑2790542

aTTGCGTTGCTGGCAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGNTGCGCGCCTTATGGGAACGCACAGGGGCGa                                                                                                                                                                                                                                       >  2:370505/1‑250 (MQ=255)

   gCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCAGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTCCCACAACTGCGGCAGGATGCTGGTTGCAACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCCAAGTGGCGAACgg                                                                                                                                                                                                                                   >  2:524441/1‑251 (MQ=255)

      ttGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCCCAGTGGCGAACGGTAt                                                                                                                                                                                                                                >  2:478016/1‑251 (MQ=255)

      gtgatGGAAAAAGGGGCCTCCCCGGCATGTCCCCTGCCGGGCCACAACAGCGGCGTTATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTGGCCTGGCGGGAAACCGCGCCACCAAAGCCGTTTGCGATCAAAACGGCATTCGCTACGACAACGGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCTCCGTATGGGAACGCACAGTGGCGAACGGTa                                                                                                                                                                                                                                 <  2:703042/246‑1 (MQ=255)

            gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcg                                                                                                                                                                                                                          >  1:268486/1‑251 (MQ=255)

             aaaaaGGGTCCGCCCCGGCCGGTCACAAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcgc                                                                                                                                                                                                                         <  2:268486/251‑1 (MQ=255)

             aaaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcgc                                                                                                                                                                                                                         >  1:606590/1‑251 (MQ=255)

               aaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAAACTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCCCGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAAAGGTATCGAGcgcg                                                                                                                                                                                                                        >  1:737791/1‑250 (MQ=255)

                  gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATg                                                                                                                                                                                                                    >  1:516194/1‑251 (MQ=255)

                  gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATg                                                                                                                                                                                                                    >  1:494370/1‑251 (MQ=255)

                   ggcccGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATgg                                                                                                                                                                                                                   <  1:607325/248‑1 (MQ=255)

                    gcccGCCCCGGCCGGCCCCCGGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGc                                                                                                                                                                                                                  <  2:91296/249‑1 (MQ=255)

                      cggCCCCGACCTGTCCCCGGACGGGCCACCACAGCGGCGTGAGCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAACCCGCGCCTCCAAAGCCGTTTGCGATCAAAACGGCATTCGCTACGACAGCTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                                                                                                                                                                <  2:750180/249‑1 (MQ=255)

                      ccgcccgcgcctctccccAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTAGTACACGGCCGGCAGCCTGAAGGCGCAGTTGTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGATCGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                                                                                                                                                                <  2:656403/235‑1 (MQ=255)

                      cccccccggctgtgcacaagccGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACCCGTCCGGCAGCCTGAAGGCTCAGGTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGGGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                                                                                                                                                                <  2:620161/230‑1 (MQ=255)

                      ccGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                                                                                                                                                                <  1:478016/251‑1 (MQ=255)

                          cccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                                                                                                                                                                            >  1:164530/1‑251 (MQ=255)

                           ccgcccTGTCACCAGGAGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCACAGTGGTGAACGGTATCGATCGCGAATGGCTTAACGc                                                                                                                                                                                                           <  2:701092/247‑1 (MQ=255)

                               ccTGCCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                                                                                                                                                                                       <  2:723181/251‑1 (MQ=255)

                                  gTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCCAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACCACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAAGGATGCGCGCCTTATGGGAACGCACAGGGGCGAACGGTATCGAGCGTGAACGGCATAACGcacacga                                                                                                                                                                                                     >  2:415647/1‑244 (MQ=255)

                                   cccccAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                                                                                                                                                                                   <  1:584018/248‑1 (MQ=255)

                                    cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                                                                                                                                                                                   <  1:459131/250‑1 (MQ=255)

                                    cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGGCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCGTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                                                                                                                                                                                                  <  1:350041/251‑1 (MQ=255)

                                        aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgc                                                                                                                                                                                              >  1:735373/1‑251 (MQ=255)

                                         gACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                                                                                                                                                                            >  2:158384/1‑236 (MQ=255)

                                         gACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                                                                                                                                                                            <  1:158384/236‑1 (MQ=255)

                                            gggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagc                                                                                                                                                                                          <  2:156922/251‑1 (MQ=255)

                                                  caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                                                                                                                                                                                    >  1:32503/1‑251 (MQ=255)

                                                   aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAcc                                                                                                                                                                                   <  1:112159/251‑1 (MQ=255)

                                                   aacaGCGGCGTGATACATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTAGAAATGGAACGGATGCGCGCCTTATGGGAACGCCCAGAGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGTCGAACTGCGGGAGCGGGAAcc                                                                                                                                                                                   >  2:81818/1‑251 (MQ=255)

                                                       gcggcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAat                                                                                                                                                                               <  1:205875/251‑1 (MQ=255)

                                                        cggcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAata                                                                                                                                                                              <  1:23722/251‑1 (MQ=255)

                                                         gtcgTGATCCATGCCGGGGTCTATGACACGTCCGGCAGCCGGAAGGCGCAGTTTCGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCTAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCTAatat                                                                                                                                                                             <  2:195748/249‑1 (MQ=255)

                                                          gcgTGATCCATGCCGGGGCCTATTACACGTCCGGCAGCCTGAAGGAGCAGTTTTGCCTGGCGGGACACCGCGCCCCCCAAGGCTTTTGCGATCACAACGGCCTTCGCTACGACAACTGCGGCAAGATGCTGGATGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTAGGGGACCGCACAGTGGCCAACGGTATCGAGCGCGAATGACTTAACGCCCACCAACTTCCCCAGCGCGCACCGAACATc                                                                                                                                                                            >  2:424853/1‑251 (MQ=255)

                                                              gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                                                                                                                                                                         <  1:246449/250‑1 (MQ=255)

                                                                   aTGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAACGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTCTGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGACCCGAATATCACCGGGCTc                                                                                                                                                                   >  1:110281/1‑251 (MQ=255)

                                                                     gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcg                                                                                                                                                                  <  1:539250/250‑1 (MQ=255)

                                                                     gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGGGGCGAACGGTATCGAGCGCGAATGGATTAACGCCGACGAACTGNGCGAGCGCGAACCGAATATCACCGGGCCcgg                                                                                                                                                                 >  2:355184/1‑251 (MQ=255)

                                                                       cGGGGTCTATTAAACGTCCGGCAGCCTGAAGGAGCATTTTTGACTGGCGGGAAACCGCGCCACCAAAACCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCGAGATGCTGGTTGCCACGTACGCTCTCGAAATGGTACGGAGGCGCGACTTATGGTAACGCACAGTGGCGAACGGTATCGAGCGAGAGTCGCTTAACCCCGAAGAACGGCGCACACGCGAAACGGATATCAACGGGCTcggcg                                                                                                                                                               >  2:454336/1‑251 (MQ=255)

                                                                         gggTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCggcggc                                                                                                                                                             <  1:485442/251‑1 (MQ=255)

                                                                            tCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt                                                                                                                                                          >  1:27085/1‑251 (MQ=255)

                                                                               atttcacGTCCGGCAGCCGGGAGGCGCAGTGTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGACGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTAGGCGGCAttttg                                                                                                                                                       <  2:226903/247‑2 (MQ=255)

                                                                               aTTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttttt                                                                                                                                                       >  1:420753/1‑251 (MQ=255)

                                                                               aTTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt                                                                                                                                                          <  1:599998/248‑1 (MQ=255)

                                                                                ctACACGTCCGGCCGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCACGATGCTGGTTGCCACGTCCGATCTCGGAATGGAACGGACGTGCGCATTATGGGAACGCACAGTGGCGAACGGTAGCGAGCGCGAATGGCTTAACGACGACGAACTGCGCGAGCGCGAACTGCATATCCCCGGGCCCGGCGGCAtttttt                                                                                                                                                      >  2:712044/2‑250 (MQ=255)

                                                                                 tACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAATGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTtgg                                                                                                                                                     >  1:700514/1‑250 (MQ=255)

                                                                                  acacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGGATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGCAACTCAACGGGCTCGGCGGCATTTTtgtg                                                                                                                                                    >  1:765057/1‑251 (MQ=255)

                                                                                         cGGCCGCCTGAAGGCGCAGGTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCa                                                                                                                                             <  2:306129/251‑1 (MQ=255)

                                                                                           gCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGc                                                                                                                                           <  2:753503/251‑1 (MQ=255)

                                                                                              gCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGc                                                                                                                                        >  1:276630/1‑251 (MQ=255)

                                                                                                  gAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAATCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGATCGGCGGCATTTTTGTGCCGTcccgagggattt                                                                                                                                    >  1:500577/1‑241 (MQ=255)

                                                                                                  gAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCCTTTTTGTGCCGTCCAGCGGCATTg                                                                                                                                    >  2:617649/1‑251 (MQ=255)

                                                                                                     ggCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTc                                                                                                                                  >  1:515119/1‑250 (MQ=255)

                                                                                                        gcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCt                                                                                                                              >  1:369877/1‑251 (MQ=255)

                                                                                                             tttGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCACGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACcg                                                                                                                          <  1:17313/250‑1 (MQ=255)

                                                                                                              ttGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACcgc                                                                                                                         <  1:308397/250‑1 (MQ=255)

                                                                                                              ttGCCGGGCGGGAAACCGCGCAACCAAAGCCTTTTGCGATCAAAACGGCAGTCGCGACGACCACTGCGGCAAGATGCTGGTTGCCACGTCCGATCACGAAACGGAACGGATGCGCGCCTTATGGTAACGCACAGTGGCGAACGGTATCGATCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCGCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGGCAGCTACcgc                                                                                                                         <  2:111458/250‑1 (MQ=255)

                                                                                                               tGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACcgcg                                                                                                                        <  1:534048/250‑1 (MQ=255)

                                                                                                                gCCTGGCGGGAAACCGCGCCCCCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGAc                                                                                                                      <  2:581673/251‑1 (MQ=255)

                                                                                                                gCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGAc                                                                                                                      <  2:138393/251‑1 (MQ=255)

                                                                                                                       gggAAACCGCTCCACCAAGGCCTTGTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAAGGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcga                                                                                                                                                                                            <  2:371066/174‑1 (MQ=255)

                                                                                                                       gggAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACCACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcga                                                                                                                                                                                            >  1:371066/1‑174 (MQ=255)

                                                                                                                           aaCCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAACGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACggcggc                                                                                                           >  1:306246/1‑251 (MQ=255)

                                                                                                                              cgcgCCACCAAAGCCTTTTGCGATCAAAACGGCAGTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGAt                                                                                                        <  2:552489/251‑1 (MQ=255)

                                                                                                                                cgcCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATgg                                                                                                      <  1:401261/251‑1 (MQ=255)

                                                                                                                                cgcCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGGCCAGCGGCATTGTCAGCTACCGCCACGCGAcggggggggtgg                                                                                                      >  2:744700/1‑242 (MQ=255)

                                                                                                                                           cctcttGCGATCAAAACGGCGTTCGCTACGACGACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACTGTATCGAGCGCGAATGGCTGAACGCCGTCGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGTCGTGACGGCGGCGATGGCAAAAATCTtc                                                                                           <  2:621806/247‑1 (MQ=255)

                                                                                                                                            cTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCCCGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGGGACGGCGGCGATGGCCAAAATCTtcc                                                                                          >  2:722938/1‑251 (MQ=255)

                                                                                                                                             ttttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtcc                                                                                          >  1:537934/1‑250 (MQ=255)

                                                                                                                                                 gCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCCGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagtcc                                                                                     >  1:734481/1‑251 (MQ=255)

                                                                                                                                                   gATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCCGCGATGGCAAAAATCTtccaggcccg                                                                                   >  2:307726/1‑246 (MQ=255)

                                                                                                                                                     tCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTtccagtccag                                                                                   >  1:63957/1‑249 (MQ=255)

                                                                                                                                                       aaaaCGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGc                                                                               <  1:524441/251‑1 (MQ=255)

                                                                                                                                                        aaaCGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCTTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCg                                                                              <  2:306246/251‑1 (MQ=255)

                                                                                                                                                         aaCGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGACAAAAATCTTCCAGTCCAGAGGCgg                                                                             >  1:511611/1‑251 (MQ=255)

                                                                                                                                                         aaCGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGAATTGTCCGCTACCGCGACGGGGCGGCGGCGATGGGAAAAATCTTCAAGTCCAGAGGCgg                                                                             >  2:552970/1‑251 (MQ=255)

                                                                                                                                                               aTTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGG‑aaa                                                                       >  1:759141/1‑250 (MQ=255)

                                                                                                                                                                   gCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGG‑AAATTATc                                                                  >  1:745350/1‑251 (MQ=255)

                                                                                                                                                                               gCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGa                                                       <  1:457740/250‑1 (MQ=255)

                                                                                                                                                                                 ggCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑GAATTATCTATAAAGccccagg                                                    >  1:322372/1‑246 (MQ=255)

                                                                                                                                                                                  gCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTc                                                   <  1:43894/251‑1 (MQ=255)

                                                                                                                                                                                  gCAAGATGCTGGTTGCCACGGCCGATCTCGAAAGGGAACGGATGGGCGCCTTTTGGTAACGCACAGTGGCGAACGGTATCGAGCGCTAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAGTTTTGTGCCGTCCAGCGGCATTGTCAGTTACCGCGACGTGGCGGCGGCGATGGCAAAAAGCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTc                                                   <  2:673315/251‑1 (MQ=255)

                                                                                                                                                                                    aaGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGACTGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATAACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGAGACGGCGGCGAGGGCAGAAATCTTGCAGTCCAAAGGCGGGGGAAATTAT‑TATaaaaccggaaga                                                   >  2:297894/1‑239 (MQ=255)

                                                                                                                                                                                    aaGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAg                                                 <  1:617649/251‑1 (MQ=255)

                                                                                                                                                                                       aTGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑GAATTTTCTATAAccccccaagtcacgg                                              >  1:640629/1‑237 (MQ=255)

                                                                                                                                                                                         gCTGGTTGCCACGGCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑c                                           <  1:504600/251‑1 (MQ=255)

                                                                                                                                                                                         gCTGGTGGCCACGGCCGATCTCGAAATGGAACGGATGCGCGCCGTAGGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCGCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCGTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑c                                           <  2:500577/251‑1 (MQ=255)

                                                                                                                                                                                          cgggTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑Ct                                          <  2:606590/249‑1 (MQ=255)

                                                                                                                                                                                            ggTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑Ctt                                         <  1:650174/250‑1 (MQ=255)

                                                                                                                                                                                            ggTTGCCACGTCCGATCTAGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGGGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGACAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑Ctt                                         <  2:511611/250‑1 (MQ=255)

                                                                                                                                                                                                       ccGAACTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGAGATGGGCCTAACGCCGACGAACTGCGCGGGCGCGAACCGAATATCACCGGGCTCGGGGGCATTTGTGTGCCGTCCAGCGGCAGTGTAAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGTG‑CTTAATGAGCATaa                              <  2:21059/250‑1 (MQ=255)

                                                                                                                                                                                                          aTCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑GAAATATCTATTAAGCCGCCGTCAGGGGGGCTTA‑TGAGGATaaaaa                           >  1:400121/1‑250 (MQ=255)

                                                                                                                                                                                                              cGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGc                       <  1:633491/250‑1 (MQ=255)

                                                                                                                                                                                                              cGAAATGGAACGGAGGCTCGCCTGATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATaaaa                            <  2:529903/245‑1 (MQ=255)

                                                                                                                                                                                                               gAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGGG‑AAATTATCTATAACGCCCAAGGCAGCGGG‑CTTAATgcggacaaaaaaagcgg                     >  2:154568/1‑235 (MQ=255)

                                                                                                                                                                                                                  aTGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCgtggt                  <  1:712044/251‑1 (MQ=255)

                                                                                                                                                                                                                     gAACGGATGCGCGCCTTATGGGAACGCACAGNGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGAt               <  2:369877/251‑1 (MQ=255)

                                                                                                                                                                                                                             gcgcgcCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGATACGTAccc       <  2:516194/251‑1 (MQ=255)

                                                                                                                                                                                                                               gcgcCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGt     <  1:497078/251‑1 (MQ=255)

                                                                                                                                                                                                                                  cctgaTGGGAACGCGCAGTGGCGAACGGTATCGAGCGCTAAGGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAg  <  2:554922/247‑1 (MQ=255)

ATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCCAGAGGCGGTG‑AAATTATCTATAACGCCGAAGTCAGCGGG‑CTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAG  >  NC_000913/2790066‑2790542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`