Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,790,153 C→T P58S (CCC→TCC)  lhgD → L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,790,1530CT96.0% 392.1 / NA 126P58S (CCC→TCC) lhgDL‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (68/52);  minor base G (3/2);  total (72/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

CCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTG  >  NC_000913/2789911‑2790398
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
ccaacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGc                                                                                                                                                                                                                                                  >  1:138393‑M2/53‑251 (MQ=255)
    cgaaaagctctattgtttccgttgggcctaaacgcagggagactccccGCGAAAAGGAATTGAGCGGATGTATGATTTTGTGATTATGGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑a                                                                                                                                                                                                                                             <  1:330246‑M2/203‑1 (MQ=255)
      aaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑a                                                                                                                                                                                                                                             >  1:604562‑M2/47‑249 (MQ=255)
          gctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAACAA‑GAAGCCGCCCCGGCCTGTCCCC‑AGACGGGCCACCAAC‑GCGGCGTGGTCCATGCCGGGGTCTTTTCCCCgtccggcagccgtaaagcgc                                                                                                                                                                                                                                        >  2:667820‑M2/43‑242 (MQ=255)
          gctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGgcgc                                                                                                                                                                                                                                       <  1:100151‑M2/209‑1 (MQ=255)
          gctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTG‑AAGgcgc                                                                                                                                                                                                                                       >  1:306129‑M2/43‑251 (MQ=255)
            tctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGgcgc                                                                                                                                                                                                                                       >  1:701092‑M2/41‑249 (MQ=255)
                 ggtttactttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGCCCTCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGCCCGCCCCGGCCTGTCGCC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATTCCGGTGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGtttt                                                                                                                                                                                                                                 <  1:582510‑M2/215‑1 (MQ=255)
                           gggcctcaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTTGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACCGCGGCGTGATCCATGCCGGGGTCTATTACACCTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGcgggg                                                                                                                                                                                                                      >  2:607325‑M2/26‑250 (MQ=255)
                           gggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCCTGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGcgggg                                                                                                                                                                                                                      >  1:184274‑M2/26‑250 (MQ=255)
                           gggcctaaacgaagggagactccccgcgcAACGGAATTGCGCGGATGTATGCTTTTGTGATTATTGGCGGCGGCATCAGCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCCGTCACC‑CGACGGGCCG‑CAACAGCGGCGGGGACCCTGCCGGGGTCCACTCCACGTCCCGCACCCTG‑AAAGCGCAGCTTTTCCCGGCggg                                                                                                                                                                                                                       >  2:457740/26‑250 (MQ=255)
                                 aaacgcagggagactccccGCGCAAAGGANTTGAGCGGATTTATGATTTTGGGATTATGGGCGGCGCCATCATCGGAATGTCGACCGCA‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATGG‑CGTTGGTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAAcc                                                                                                                                                                                                                 <  2:380753‑M2/230‑1 (MQ=255)
                                     gcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGACC‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTAAACGTCCAGCAGCCTT‑CAAGCGCAGTTTTGCCTGGCGGG‑CAACCCCGcc                                                                                                                                                                                                            >  2:401261‑M2/16‑250 (MQ=255)
                                      cagagagcctccccGCGCAAAGGAATTGAGCGGATGTTTGATTTTGTGATTATTGGCGGCGGCATCATCGTCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTACCCGGACGCCCACATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCCCC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGccac                                                                                                                                                                                                          <  1:357869‑M2/237‑1 (MQ=255)
                                       agggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGccacc                                                                                                                                                                                                         >  1:47542‑M2/14‑251 (MQ=255)
                                             actccccGCGCAAAGGAATTGAGCGGATGTCTGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑CTGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCC‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AACGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCCCCAAAG‑cc                                                                                                                                                                                                  >  1:673315‑M2/8‑251 (MQ=255)
                                                 cccgcccaaggaaatttgccGGATGTATGATTTTGTGATTATTGGCGGCGGCTTCAGCGGCATGCCGACCGCT‑GTGCAACTGTTTG‑ATGTCTATCCGGACGCCCGCATTG‑CTTTGCTGAAAAAA‑GAGTCCGCCCCGTCCTGTCCCC‑AAACGGTCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCTGG‑AAACCGCGCCACCAAAG‑CCtttt                                                                                                                                                                                              <  1:302366/232‑1 (MQ=255)
                                                   cgcgcAATGGAAGTGAGCGGATGTAGGATGGTGTGATTATGCGCGGCGACATCGTGGGCATGTCGACCCCC‑AGGCATCTGATTG‑ATGTCTACCCGTCCGCCCGCATTG‑CGTTGCGGGAAAAA‑GAGTCCGCCCCGCCCTGCCACC‑GGACGGTCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGc                                                                                                                                                                                            <  2:333307/250‑1 (MQ=255)
                                                   cGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGc                                                                                                                                                                                            >  1:712340‑M2/2‑251 (MQ=255)
                                                      gcAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCggt                                                                                                                                                                                         >  2:501322/1‑249 (MQ=255)
                                                           ggAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGGGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGGTCaaa                                                                                                                                                                                     >  2:539250/1‑250 (MQ=255)
                                                           ggAATTGAGCGGATGTATGATTTTGTGATTATGGGCGGCGGCATCATCGGATTGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCGGCATGG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGCCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCaaaa                                                                                                                                                                                    <  1:476621/251‑1 (MQ=255)
                                                            gAATTGGGCGGAGTTATGAGTTTGTGGTTATTGGCGGCGGCATCATCGGCATGTCGCCCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCTTTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAAc                                                                                                                                                                                   <  2:178939/251‑1 (MQ=255)
                                                             aaTTGAGCGGATGTATGATTTTGTGATGATTGGGGGGGGCATCATCGGCATGTCGACCGCC‑ATGCAACGGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCGGGAAAAA‑GAGCCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACg                                                                                                                                                                                  <  1:509701/251‑1 (MQ=255)
                                                                 gagcggtggTATGATTTTGTGATTTTGGGCGGCGCCATCCGCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGCCGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGCCGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCAt                                                                                                                                                                              <  2:667576/243‑1 (MQ=255)
                                                                 gAGCGGATGTATGATTTTGTGATTATTGGAGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCCAAG‑CCTTTTGCGATCCAAACGGCAt                                                                                                                                                                              >  1:620161/1‑251 (MQ=255)
                                                                  ggCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGCCCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCAtt                                                                                                                                                                             <  1:667820/250‑1 (MQ=255)
                                                                  aGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATGATCGGCATTGCGACCGAC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑AGGTGCTGGAAAAA‑GGGACCGCCCCGGCCTGTCTCC‑AGACGGGCCA‑CACCAGCGGCGTGATGCATGCCGGGGGCTATTACACGAACGGCACCCTA‑AAGGCGCAGATTTGCCTGGCGGG‑AAACCGCGCCACCAAAT‑CCTTTTGCGATAAAAGCGGCAtt                                                                                                                                                                             >  1:683080/1‑251 (MQ=255)
                                                                  aGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCAtt                                                                                                                                                                             >  1:497728/1‑251 (MQ=255)
                                                                   gCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑GAACCGCGCCCCCCAAG‑ccaaatcgggagagcgtcgggt                                                                                                                                                                              >  2:471922/1‑229 (MQ=255)
                                                                    cGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGCAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGGCCGGCAGCCCG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCNCCAAAG‑CCTTTTTCGGTCAAACAGGCATTCg                                                                                                                                                                           >  2:350041/1‑251 (MQ=255)
                                                                      gATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCA‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                                         <  1:744695/251‑1 (MQ=255)
                                                                       attattattttggggATTATTGGCGGCGGCNTCACCGGCATGGCG‑CCGCCCAGCCAACT‑ATTGGATGTCAACCCGG‑CGCCCCGATTG‑GGGTGCCGGAAAAAAGAGTCCGCCCCGGCCGGTCACC‑GGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCGGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                                         <  2:342144/236‑1 (MQ=255)
                                                                       aTGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                                         >  1:627660/1‑250 (MQ=255)
                                                                       aTGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCACGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGACACCAAAG‑CCATTTGCGATCAAAACGGCATTAGCt                                                                                                                                                                         >  1:111458/1‑250 (MQ=255)
                                                                        tGTATGATTTTGTGATTATTGGCGGCGGCATCCTCGGCATGGCTACCGCC‑ATGCAACTGATTG‑ATGTCTCTCCGGGCCCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGACCCGGCCTGTCACC‑AGGCGGGCCA‑CAACAGAGGCGGGATCCCAGCCGGGGGCTATTACACGTCCGGTAGCCTGCAAGG‑GCAGTTTTGCCAGGGGGG‑AAACCCCGCCCACAAAG‑CCTATTGCGAGCAAAAccgccatcgcta                                                                                                                                                                        >  2:504600/1‑239 (MQ=255)
                                                                              aTTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTTCCTTGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAAAGGCCTTCGCTACGACAAc                                                                                                                                                                 >  1:377548/1‑251 (MQ=255)
                                                                              aTTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAAc                                                                                                                                                                 >  1:290092/1‑251 (MQ=255)
                                                                                tttGTGATTATTGGGGGCGGCACCATCGGCATGTCGTCCGCC‑ATGCAACGGATTG‑ATGTCTACCCGGACGCCCGCATTG‑CGTGGCGGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACt                                                                                                                                                                <  2:627660/250‑1 (MQ=255)
                                                                                tttGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGCCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑GAACCGCGCCACCCAAG‑CCTTTTGCGATCAAAACGGCATTCGcctcggcaactg                                                                                                                                                               >  2:82034/1‑240 (MQ=255)
                                                                                   gtgATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCgg                                                                                                                                                            >  1:552489/1‑251 (MQ=255)
                                                                                      attattGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCa                                                                                                                                                          >  1:549103/1‑250 (MQ=255)
                                                                                         attGGCGGCGGCATCCTCGGCATGGCGACCGCC‑CTGCAACTTATTG‑ATGTCTATCCGGACGCCCCCCTTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCC‑CAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGGCCGGCAACCCG‑AAGGCGCAGTTTTTCCCGGCGGG‑GAACCGCACCCCCAAAGACCTTTGGC‑ATCAAAACGGCACTCGCTACGACAACTGccgcc                                                                                                                                                          >  2:246449/1‑243 (MQ=255)
                                                                                           tGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGc                                                                                                                                                    >  1:156922/1‑251 (MQ=255)
                                                                                            ggcggcggcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCt                                                                                                                                                   >  1:295629/1‑251 (MQ=255)
                                                                                            ggcggcggcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCt                                                                                                                                                   >  1:9281/1‑251 (MQ=255)
                                                                                            ggcggcggcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑ACTTTTGCGATCAAAACGGCATTCGCCACGGCAAcctcggacagatgcc                                                                                                                                                   >  2:534048/1‑237 (MQ=255)
                                                                                            ggcggcggcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCCCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCCAGAttct                                                                                                                                                   >  2:737124/1‑248 (MQ=255)
                                                                                                   gcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGcc                                                                                                                                            <  1:269060/251‑1 (MQ=255)
                                                                                                   gcATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGCCCGCCCCGGCCTGCCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGcc                                                                                                                                            <  1:82034/251‑1 (MQ=255)
                                                                                                     atcatcGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGCTCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGCTCAACACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGacac                                                                                                                                          >  2:308397/1‑251 (MQ=255)
                                                                                                        atcGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGGCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CCACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCCG‑AAGGCGCAGTTTTGCCTGGCGGGGAAACC‑CGCCACCCAAC‑CCTTTTTCCGTCAAAACGGCATTCGCTACGACCAATGCGGCCAGaatctgggtgcaacggc                                                                                                                                       >  2:23722/1‑235 (MQ=255)
                                                                                                                gTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCg                                                                                                                               >  1:710959/1‑251 (MQ=255)
                                                                                                                      cGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCCCC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATgg                                                                                                                         <  1:93291/251‑1 (MQ=255)
                                                                                                                       gCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATgg                                                                                                                         >  1:72484/1‑250 (MQ=255)
                                                                                                                              cAACTGATTG‑ATGTCTATCCGGACCCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTT‑AAGGCGCAGTTTTGCCTGGCGGT‑ATACCGCGCCACCAAAG‑CCTTTTGCGATCAAATCGGCATTCGCGGCGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAt                                                                                                                  <  1:699380/251‑1 (MQ=255)
                                                                                                                                       g‑atGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCtt                                                                                                         >  1:622644/1‑251 (MQ=255)
                                                                                                                                           gTCTCTCCGGACGCC‑GCCTTGGCGTGGCGGGAAAAA‑GAGTCCGCCCCGGCCTGTCCCC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATg                                                                                                      <  2:72484/251‑1 (MQ=255)
                                                                                                                                           gTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATg                                                                                                      >  1:554922/1‑251 (MQ=255)
                                                                                                                                               aTCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGa                                                                                                   >  2:205875/1‑250 (MQ=255)
                                                                                                                                                  cGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                                               <  1:501322/251‑1 (MQ=255)
                                                                                                                                                  cGGACGCCCGCATGG‑CGTTGCTGGAAAAA‑GAGCCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                                               <  1:599963/251‑1 (MQ=255)
                                                                                                                                                      ccgcccggattgggttgtagaaaaaa‑aAGCCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcaca                                                                                            <  2:712340/230‑1 (MQ=255)
                                                                                                                                                             aTTG‑CGTTGCTGGCAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGNTGCGCGCCTTATGGGAACGCACAGGGGCGa                                                                                     >  2:370505/1‑250 (MQ=255)
                                                                                                                                                                g‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAGACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                                 >  1:347248/1‑203 (MQ=255)
                                                                                                                                                                g‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCAGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTCCCACAACTGCGGCAGGATGCTGGTTGCAACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCCAAGTGGCGAACgg                                                                                 >  2:524441/1‑251 (MQ=255)
                                                                                                                                                                    ttGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCCCAGTGGCGAACGGTAt                                                                              >  2:478016/1‑251 (MQ=255)
                                                                                                                                                                    gtgatGGAAAAA‑GGGGCCTCCCCGGCATGTCCCC‑TGCCGGGCCA‑CAACAGCGGCGTTATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTGGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCGTTTGCGATCAAAACGGCATTCGCTACGACAACGGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCTCCGTATGGGAACGCACAGTGGCGAACGGTa                                                                               <  2:703042/246‑1 (MQ=255)
                                                                                                                                                                       cgggAAAAA‑GGGCCCCCCCCGGCC‑GGCACCCAGCCGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGa                                                                           <  2:549103/249‑1 (MQ=255)
                                                                                                                                                                          gAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcg                                                                        >  1:268486/1‑251 (MQ=255)
                                                                                                                                                                             aaa‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAA‑ACTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCCCGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAAAGGTATCGAGcgcg                                                                      >  1:737791/1‑250 (MQ=255)
                                                                                                                                                                                 gagTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATg                                                                  >  1:516194/1‑251 (MQ=255)
                                                                                                                                                                                  ggcccGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATgg                                                                 <  1:607325/248‑1 (MQ=255)
                                                                                                                                                                                  cgcccGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATgg                                                                 <  2:407505/248‑1 (MQ=255)
                                                                                                                                                                                     cggCCCCGACCTGTCCCC‑GGACGGGCCA‑CCACAGCGGCGTGAGCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AACCCGCGCCTCCAAAG‑CCGTTTGCGATCAAAACGGCATTCGCTACGACAGCTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                              <  2:750180/249‑1 (MQ=255)
                                                                                                                                                                                     ccGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                              <  1:478016/251‑1 (MQ=255)
                                                                                                                                                                                      cccccccggctgtgcacaagccGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACCCGTCCGGCAGCCTG‑AAGGCTCAGGTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGGGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                              <  2:620161/230‑1 (MQ=255)
                                                                                                                                                                                         cccGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                          >  1:164530/1‑251 (MQ=255)
                                                                                                                                                                                          ccgcccTGTCACC‑AGGAGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCACAGTGGTGAACGGTATCGATCGCGAATGGCTTAACGc                                                         <  2:701092/247‑1 (MQ=255)
                                                                                                                                                                                              ccTGCCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                                     <  2:723181/251‑1 (MQ=255)
                                                                                                                                                                                                   cACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                                 <  1:459131/250‑1 (MQ=255)
                                                                                                                                                                                                   cACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGGCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCGTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                                                <  1:350041/251‑1 (MQ=255)
                                                                                                                                                                                                        aGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgc                                            >  1:735373/1‑251 (MQ=255)
                                                                                                                                                                                                         gACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                          >  2:158384/1‑236 (MQ=255)
                                                                                                                                                                                                         gACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACg                                                          <  1:158384/236‑1 (MQ=255)
                                                                                                                                                                                                            gggCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagc                                        <  2:156922/251‑1 (MQ=255)
                                                                                                                                                                                                                    aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAcc                                 <  1:112159/251‑1 (MQ=255)
                                                                                                                                                                                                                    aacaGCGGCGTGATACATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTAGAAATGGAACGGATGCGCGCCTTATGGGAACGCCCAGAGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGTCGAACTGCGGGAGCGGGAAcc                                 >  2:81818/1‑251 (MQ=255)
                                                                                                                                                                                                                        gcggcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAat                             <  1:205875/251‑1 (MQ=255)
                                                                                                                                                                                                                         cggcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAata                            <  1:23722/251‑1 (MQ=255)
                                                                                                                                                                                                                           gcgTGATCCATGCCGGGGCCTATTACACGTCCGGCAGCCTG‑AAGGAGCAGTTTTGCCTGGCGGG‑ACACCGCGCCCCCCAAG‑GCTTTTGCGATCACAACGGCCTTCGCTACGACAACTGCGGCAAGATGCTGGATGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTAGGGGACCGCACAGTGGCCAACGGTATCGAGCGCGAATGACTTAACGCCCACCAACTTCCCCAGCGCGCACCGAACATc                          >  2:424853/1‑251 (MQ=255)
                                                                                                                                                                                                                               gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                       <  1:246449/250‑1 (MQ=255)
                                                                                                                                                                                                                                    aTGCCGGGGTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAACG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTCTGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGACCCGAATATCACCGGGCTc                 >  1:110281/1‑251 (MQ=255)
                                                                                                                                                                                                                                        cGGGGTCTATTAAACGTCCGGCAGCCTG‑AAGGAGCATTTTTGACTGGCGGG‑AAACCGCGCCACCAAAA‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCGAGATGCTGGTTGCCACGTACGCTCTCGAAATGGTACGGAGGCGCGACTTATGGTAACGCACAGTGGCGAACGGTATCGAGCGAGAGTCGCTTAACCCCGAAGAACGGCGCACACGCGAAACGGATATCAACGGGCTcggcg             >  2:454336/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gggTCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCggcggc           <  1:485442/251‑1 (MQ=255)
                                                                                                                                                                                                                                             tCTATTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt        >  1:27085/1‑251 (MQ=255)
                                                                                                                                                                                                                                                atttcacGTCCGGCAGCCGG‑GAGGCGCAGTGTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGACGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTAGGCGGCAttttg     <  2:226903/247‑2 (MQ=255)
                                                                                                                                                                                                                                                aTTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAttttt     >  1:420753/1‑251 (MQ=255)
                                                                                                                                                                                                                                                aTTACACGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt        <  1:599998/248‑1 (MQ=255)
                                                                                                                                                                                                                                                   acacGTCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGGATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGCAACTCAACGGGCTCGGCGGCATTTTtgtg  >  1:765057/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        
CCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCC‑ATGCAACTGATTG‑ATGTCTATCCGGACGCCCGCATTG‑CGTTGCTGGAAAAA‑GAGTCCGCCCCGGCCTGTCACC‑AGACGGGCCA‑CAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTG‑AAGGCGCAGTTTTGCCTGGCGGG‑AAACCGCGCCACCAAAG‑CCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTG  >  NC_000913/2789911‑2790398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑