Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,476,629	A→G	intergenic (+119/+65)	torI → / ← dsdC	prophage CPS‑53 recombination directionality factor and response regulator inhibitor/DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,476,629	0	A	G	100.0%	189.4 / NA	52	intergenic (+119/+65)	torI/dsdC	prophage CPS‑53 recombination directionality factor and response regulator inhibitor/DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

CATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATC  >  NC_000913/2476387‑2476874                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      cATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaa                                                                                                                                                                                                                                                <  1:178621/250‑1 (MQ=255)  aTCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATACACGGGGGAGCAAGATGGTTATATCGTGACCATTGTGAATTCACAAATAAGCTCTTACGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCATTCAATCCCCTGCTGCTTCACGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaat                                                                                                                                                                                                                                              >  1:629967/1‑251 (MQ=255)      aTGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATACGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaattaa                                                                                                                                                                                                                                           >  1:302953/1‑250 (MQ=255)          ccGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCg                                                                                                                                                                                                                                      <  1:389352/251‑1 (MQ=255)                tAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTa                                                                                                                                                                                                                                 <  1:184938/250‑1 (MQ=255)                      aGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGcc                                                                                                                                                                                                                          <  1:98159/251‑1 (MQ=255)                         cGACCTGCAAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATAAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGa                                                                                                                                                                                                                        >  1:579133/1‑250 (MQ=255)                                          aaGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAAc                                                                                                                                                                                                      <  1:180712/251‑1 (MQ=255)                                                         gAGCAAGATGGTTATAACGTGACCATTGTGTATTCAAACATAAGCTCTTAAGCCGCGCCAATGGGAAAAATAGCGGGTAACATATTTTTAACATCTCAAAAACACCATTCAAATCAATCACCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATACTTGGGATTAGCCCTAAAACCACATAAAGACCGAAAATTAAATTGAAACTAGCATGCCGAATAAGGCACACCAGGCAATCGTGGTTTTATTc                                                                                                                                                                                        >  2:410716/1‑250 (MQ=255)                                                           gCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCGCTTAAGCCGCGCCAATGGGTAAAAGAGCGGGGAAAATATTTCTCACATCTAAAAAACCCCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCAtt                                                                                                                                                                                     <  2:9569/251‑1 (MQ=255)                                                                  ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAg                                                                                                                                                                               <  1:124768/250‑1 (MQ=255)                                                                  ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCAGTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGt                                                                                                                                                                              <  2:44/251‑1 (MQ=255)                                                                     tataTCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGt                                                                                                                                                                           >  1:617728/1‑251 (MQ=255)                                                                           gTGACCATTGTGAATTCAAAAATAAGCTCGTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAACAACACCATTCCAATCAATCCCCTGCTGCGTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGATCTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAGTCATTATGAAGTGGTTTTTAc                                                                                                                                                                     <  2:244354/251‑1 (MQ=255)                                                                                    gtgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAAAACCATTCCAATCCATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTTGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTctct                                                                                                                                                            >  1:708558/1‑251 (MQ=255)                                                                                     tgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCtt                                                                                                                                                           >  1:298772/1‑251 (MQ=255)                                                                                        aTTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGc                                                                                                                                                        >  1:234203/1‑251 (MQ=255)                                                                                          tCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                                                                                                                                                       >  1:474745/1‑250 (MQ=255)                                                                                           cAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTGTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAg                                                                                                                                                     <  2:160856/251‑1 (MQ=255)                                                                                             aaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGat                                                                                                                                                   <  1:642163/251‑1 (MQ=255)                                                                                                    gCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCCTTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAAc                                                                                                                                            >  2:231612/1‑251 (MQ=255)                                                                                                    gCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAAc                                                                                                                                            <  1:459182/251‑1 (MQ=255)                                                                                                      tctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGc                                                                                                                                          >  1:664418/1‑251 (MQ=255)                                                                                                          aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCATGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTc                                                                                                                                       <  2:238950/250‑1 (MQ=255)                                                                                                             ccGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAACCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCATATAATAAACGCTTCAAtt                                                                                                                                   >  2:10187/1‑251 (MQ=255)                                                                                                              cgcgCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCaactt                                                                                                                                  >  1:304681/1‑248 (MQ=255)                                                                                                                cgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAAttttt                                                                                                                                <  1:649649/251‑1 (MQ=255)                                                                                                                 gcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTg                                                                                                                               <  2:302953/251‑1 (MQ=255)                                                                                                                        ggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCActg                                                                                                                        >  1:481921/1‑251 (MQ=255)                                                                                                                        ggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCActg                                                                                                                        >  2:238121/1‑251 (MQ=255)                                                                                                                           aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATACCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCActgcct                                                                                                                     >  2:241043/1‑251 (MQ=255)                                                                                                                            aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCActgcctg                                                                                                                    <  1:568950/251‑1 (MQ=255)                                                                                                                                       aaaaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAAGAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAAtgt                                                                                                         <  1:202086/251‑1 (MQ=255)                                                                                                                                                  cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGta                                                                                                <  2:190408/249‑1 (MQ=255)                                                                                                                                                       cTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGc                                                                                         >  1:369505/1‑251 (MQ=255)                                                                                                                                                        aaaaaaaCCCCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCt                                                                                        <  2:708558/250‑1 (MQ=255)                                                                                                                                                         aaaaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTaataat                                                                                           <  1:245074/247‑1 (MQ=255)                                                                                                                                                            aaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATg                                                                                    <  1:238121/251‑1 (MQ=255)                                                                                                                                                                     tCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCAc                                                                           >  2:152577/1‑251 (MQ=255)                                                                                                                                                                      gcaatcaatCCCCTGCTGCTGCAAGTAGATGTCTGCAGGGAACCCCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCTGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTAGGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAGTAAACGCTTCAATTTTGGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCAcc                                                                          <  2:467693/250‑1 (MQ=255)                                                                                                                                                                           caatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCat                                                                     <  2:474745/251‑1 (MQ=255)                                                                                                                                                                             atcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCatat                                                                   >  1:251318/1‑251 (MQ=255)                                                                                                                                                                             atcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCatat                                                                   >  2:529815/1‑251 (MQ=255)                                                                                                                                                                                cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGc                                                                >  1:653755/1‑251 (MQ=255)                                                                                                                                                                                cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGATTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATCTCGc                                                                >  1:167212/1‑251 (MQ=255)                                                                                                                                                                                                                cacCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGACGACTAAGGCACACCAGGAACTCGTGGTTTTATTCTTTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATACTAAACGCTTAAATTTTTGGACACTGACTGACAGGTAATGTGGAGATGTAATCATGCTGATGGCATTTCACCGTAATATCGCAAAAAGGAGCGACGAGCTCACAAATGGCAAGc                                >  1:368381/1‑251 (MQ=255)                                                                                                                                                                                                                         tgtgTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCtttttt                         <  1:693020/249‑1 (MQ=255)                                                                                                                                                                                                                                   ccTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGtt             >  1:549332/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaaCCACATAAAGACCGAAAATTAAATTCGAACTAGCAGCACGAATAAGGCACACCAGGAACTCGTGGCTTTATTCATTATGAAGTGGTTTATACCTGTTCCCTTAGCCAGCTAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTAACCGTCATATCGCCAAACGTCGCGCAGAGCTCACCACTGGCACGCCTTTTTTGTACCAGGCGtttcc          >  2:646745/1‑249 (MQ=255)                                                                                                                                                                                                                                        aaCCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACAAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTA‑TTATTCTGATGGCATTTCACCGTCATATCGCCAAAACGCCCGACGAGCCCACCACTGGCAAGCCTTTTTTTTACCAGGCGTTttcttc       >  1:495569/1‑251 (MQ=255)                                                                                                                                                                                                                                         aCCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTttcttc       >  1:354609/1‑251 (MQ=255)                                                                                                                                                                                                                                              aTAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATc  <  1:43188/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATC  >  NC_000913/2476387‑2476874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑