Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	214.9 / NA	61	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/29);  total (32/29)

CAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACA  >  NC_000913/2112932‑2113417                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cttggacattacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGATAACTgcgtgcat                                                                                                                                                                                                                                             >  2:714952‑M2/136‑251 (MQ=255)                   cggtcaggtgggattaccgcgctgaatgcgcgcggcctggaatgttcgcaaagaagtatccaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGGGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGc                                                                                                                                                                                                                           <  2:240731‑M2/134‑1 (MQ=255)                                   ccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCt                                                                                                                                                                                                           <  1:289221‑M2/150‑1 (MQ=255)                                     gcgctgaatgctcgcggcttggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCAGGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTaa                                                                                                                                                                                                         <  1:653897‑M2/152‑1 (MQ=255)                                        ctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAAcc                                                                                                                                                                                                      >  2:524305‑M2/97‑251 (MQ=255)                                              gctcgcggcctggaatgttcgcaaataagtattcaaattctttattaaagtaaaaatagcttatccatgcttataggcttacggctttatTTATGCCATATTCCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaaaa                                                                                                                                                                                                <  1:524305‑M2/161‑1 (MQ=255)                                                  gtggcctggaatgttcgtaaataagtatacaatgtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTCCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTTATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTc                                                                                                                                                                                            <  2:578566‑M2/165‑1 (MQ=255)                                                   cggcctgaaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTGAAGAGCATGCGGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTCCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCGAAACCCGAAAAATTc                                                                                                                                                                                            <  2:561004‑M2/165‑1 (MQ=255)                                                    ggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCa                                                                                                                                                                                           >  1:90505‑M2/85‑250 (MQ=255)                                                      acctgcgggaagagacataagtagacaaagtncttattaaattataaatagcttatccatgcttatatacttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCGACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAc                                                                                                                                                                                        <  2:352938‑M2/169‑1 (MQ=255)                                                                   caaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTACTTGTCtt                                                                                                                                                                            >  2:318252‑M2/70‑250 (MQ=255)                                                                   caaacaagtatacaaagtacttattaaagtataaatagcttaccctggcttatatgcttacggctttatTTATTCCATATTAATACAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGATCGTTACCCACGCGCGCTAAACCCGAAAAATTCAAACGCTTATTTTCTTa                                                                                                                                                                           <  2:720214‑M2/182‑1 (MQ=255)                                                                    aaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAc                                                                                                                                                                          >  2:495789‑M2/69‑251 (MQ=255)                                                                     aataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                                         <  1:512922‑M2/184‑1 (MQ=255)                                                                     aataaggttacaaagtacttattaaagtataaatagcttttccatgcttatatgcttacggctttatTTATTCCATATTACGTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                                         <  2:480312‑M2/184‑1 (MQ=255)                                                                            atacaaagaacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGCTGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATACGc                                                                                                                                                                  >  2:431400‑M2/61‑251 (MQ=255)                                                                                       ttattaaagtataaatagcttatcaatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACAGCCCCTGGCTTTACAACTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACAAATCCGCTCTGGAAACa                                                                                                                                                        >  2:168161‑M2/50‑250 (MQ=255)                                                                                            aaagtatgaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGaa                                                                                                                                                   >  1:719280‑M2/45‑250 (MQ=255)                                                                                               gtataaatagcttatcaatgctaatatgctaacggctgtatTTATTTCATATTACTTCAGAGCATGCTGATAAATAAGCGGCTCTCA‑GTTTGAGTAATAGTGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAGCGGTAATTGTCTTGCCAAACCGCTCTGGAAACAAGGAAAATc                                                                                                                                               <  2:469295‑M2/210‑1 (MQ=255)                                                                                                    aatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGACTTACCAATCCGCTCTGGAAACAAGGAAAATCCTgg                                                                                                                                           >  2:432705‑M2/37‑250 (MQ=255)                                                                                                    aaatgctaatccatgcttttatgtttacggctttatTTATACCATATTCCTTCAGAGCTTGTGGTGAAATAATCGGCTCTCA‑GTTTAATTAAGGGAGGTATTAATGCACCCTACCGCCCCTGGCTTTACAGCTACCAGAGCACAGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGGCTTACCAATCCGCTCTGGAAACAAGGATAATCCTGGa                                                                                                                                          <  2:487811‑M2/215‑1 (MQ=255)                                                                                                     aaagcttctccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCAAGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAACCCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAAtccttga                                                                                                                                          >  2:187593‑M2/36‑247 (MQ=255)                                                                                                        gcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAAc                                                                                                                                      >  1:78836‑M2/33‑251 (MQ=255)                                                                                                              ccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGa                                                                                                                                 >  1:83750‑M2/27‑250 (MQ=255)                                                                                                                   cttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaaa                                                                                                                            >  1:531758‑M2/22‑250 (MQ=255)                                                                                                                        atgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCt                                                                                                                       >  2:9555‑M2/17‑250 (MQ=255)                                                                                                                         ttcttacgtttttttttATTCCTTATTTCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCT‑GTTTGATTAATAGGGATATTAATGCCCGCTACCGCCCCTTGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTAGGATGTGACGTGCGTTACCCACGCGCGCATAACCCGAAAAGTTCAAACGCTAATTGTCTTACCAATCCTCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTa                                                                                                                      <  2:750101/236‑1 (MQ=255)                                                                                                                         ggcttacggctttatTTATTCCTTATTACTCCAGAGCTTGATGGGAAATAAGCGTCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGTCTTTACAGCTACCAGATCATTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCTCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTa                                                                                                                      <  2:669897‑M2/235‑1 (MQ=255)                                                                                                                              acggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCt                                                                                                                 <  1:625443‑M2/240‑1 (MQ=255)                                                                                                                               cggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCGAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAACCTTTGAATAAAACCCTACTGCTa                                                                                                                >  1:309861‑M2/10‑250 (MQ=255)                                                                                                                                    ttatTTATTCCATATTACTGCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTc                                                                                                            <  1:538824‑M2/245‑1 (MQ=255)                                                                                                                                        ttATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAACCTTTGAATAAAACCCTACTGCTAACTCgttttt                                                                                                      >  2:447749/1‑248 (MQ=255)                                                                                                                                        ttATTCCAGATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACCCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgttgt                                                                                                       <  2:76765/250‑1 (MQ=255)                                                                                                                                          aTTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACACTACTGCTAACTCGTTGTTAt                                                                                                    >  1:423726/1‑251 (MQ=255)                                                                                                                                           ttCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGAAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTAtt                                                                                                   >  2:759399/1‑251 (MQ=255)                                                                                                                                           ttCCATATTACTTCAGAGCATGCTGCGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGAATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATACGCTCTGGAAACAAGGAACATCCTGGAAAACTTTGAATAAAACCCTACTGCGAATCCgttgttctt                                                                                                   >  2:648089/1‑248 (MQ=255)                                                                                                                                            tccatatttcgacagggCATGCTGTTAAATCAGCTGCACTCATGTTT‑ATTGAGAGCGGTATTCATGCCCGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTAtt                                                                                                   <  2:554030/234‑1 (MQ=255)                                                                                                                                            tCCATATTACTTCGGAGCATGCTGTGAAATAAGCGGCTCTCC‑GTTTGATTAATAGAGGTATTACTGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATACTGGCAAACTTTGAATACAACCCTACTGCTAATTCGTTGTTATTc                                                                                                  >  2:728861/1‑251 (MQ=255)                                                                                                                                                 atTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCATATGATGTGACGAGCGTTACCCACTCGCGCTAACCCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGCAAACTTTGAATAAAACACTACTGCTAACTCGTTGTTATTCTGATg                                                                                             >  1:682169/1‑251 (MQ=255)                                                                                                                                                  tttcTTCAGAGCATGCTGTTAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTATACCCGAAAAATTCAATCGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATg                                                                                             <  2:423726/247‑1 (MQ=255)                                                                                                                                                    aCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGt                                                                                           <  1:558868/250‑1 (MQ=255)                                                                                                                                                          gagCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTtatat                                                                                     >  1:601119/1‑250 (MQ=255)                                                                                                                                                            gCATGCTATGAAATAAGCGGTCCTCA‑GTGTGATAAATAGATGTATTTATGACCGGTACCGCCCCTGGCTTTACAGATACCAGAGCACTGCGTGCATGCCTAGGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTTCCAAGCCGCTCTTGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAATCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATaaa                                                                                  <  2:506476/251‑1 (MQ=255)                                                                                                                                                                 cTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTCTTCTGATGGTTTATATAAaacaac                                                                             >  2:496928/1‑251 (MQ=255)                                                                                                                                                                   gtgAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                                             <  1:495789/249‑1 (MQ=255)                                                                                                                                                                            gCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCACTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGGTGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGa                                                                      >  1:599830/1‑247 (MQ=255)                                                                                                                                                                              ggCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTCTTCTGATGGTTTATCTACAACAACGGCATGACGAttt                                                                >  1:371541/1‑250 (MQ=255)                                                                                                                                                                                         ttGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTTCCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTATTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAAt                                                       <  1:431400/250‑1 (MQ=255)                                                                                                                                                                                           gATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAACCTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATg                                                                                             >  2:225340/1‑210 (MQ=255)                                                                                                                                                                                           gATTAATAGAGGTATTAAGGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATg                                                                                             <  1:225340/210‑1 (MQ=255)                                                                                                                                                                                                               cACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTTCGAATTTTCACt                                <  2:83750/251‑1 (MQ=255)                                                                                                                                                                                                                    tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                                             >  1:112534/1‑201 (MQ=255)                                                                                                                                                                                                                    tACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                                             <  2:112534/201‑1 (MQ=255)                                                                                                                                                                                                                        gCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTtat                        >  1:633819/1‑250 (MQ=255)                                                                                                                                                                                                                         ccccTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGTCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATaa                      <  1:446126/251‑1 (MQ=255)                                                                                                                                                                                                                          cccTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGCCGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAAt                     >  1:276512/1‑251 (MQ=255)                                                                                                                                                                                                                               gCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCt                >  1:226945/1‑251 (MQ=255)                                                                                                                                                                                                                               gCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCt                >  1:385549/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGTTTTATATAAAACAACGTCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATc            <  2:434244/250‑1 (MQ=255)                                                                                                                                                                                                                                         tACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGt      >  2:106593/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTa    <  1:318252/251‑1 (MQ=255)                                                                                                                                                                                                                                              gagCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAACCCCGAAAAATTCAAACGCTAATTGTCTTACCAATCAGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGCAGATTTGCAAAAAATTACTTTTTCTGCTAATTTTCACTCCCGATATAATTTTCTTATAAACCGTTACa  >  2:551813/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCA‑GTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACA  >  NC_000913/2112932‑2113417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑