Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,851,841	C→T	Q328* (CAA→TAA)	ansA →	L‑asparaginase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,851,841	0	C	T	100.0%	284.7 / NA	75	Q328* (CAA→TAA)	ansA	L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/42);  total (33/42)

AATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTG  >  NC_000913/1851595‑1852085                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaTGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTaaaa                                                                                                                                                                                                                                                  <  2:458982/251‑1 (MQ=255)   tGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCCGATAAGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGTTACTAAAAACATTCGCAAGGCCATGAGATAAAAcc                                                                                                                                                                                                                                                >  2:499972/1‑251 (MQ=255)      gcCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGGTATTACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGGTACTGAAACCATTCGCAAGGCCCTGAGCTAAAACCTgc                                                                                                                                                                                                                                             >  2:171549/1‑251 (MQ=255)           aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCgcggc                                                                                                                                                                                                                                        <  1:636916/251‑1 (MQ=255)                aaaGCCTGCCTGCAGTAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCGACCGTACACAATGTATGTCCGGTAAAGTGAACATTGGTGGTTATGCCACCGGTAACGCCCTCTCCCATGCCGGCGTAATTGGCGGTGCATATATGACTGTAGAAGCCACGCTAACCAAACTGCCGTGCCTGCTTAGCCAGGAACTGTATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGTCGatcg                                                                                                                                                                                                                                   <  2:92254/251‑4 (MQ=255)                    ccTTGCTGCAGGAATTACAAGATGCCAGCGATCGCGGTATTGTTGTGGGCAACCTGACACAATTGATGTCCGGTAAAGTGAACATGGGTGTTTATGCCACCGGTACCGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGATGCCACGCTAACCAATCTGCATTGCCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGAc                                                                                                                                                                                                                                <  2:193413/250‑1 (MQ=255)                     cTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGc                                                                                                                                                                                                                              >  2:186174/1‑251 (MQ=255)                      ttCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCt                                                                                                                                                                                                                             >  1:99053‑M1/1‑250 (MQ=255)                           gCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttg                                                                                                                                                                                                                          <  1:377353‑M1/249‑5 (MQ=255)                            cAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctc                                                                                                                                                                                                                       <  1:258823‑M1/251‑8 (MQ=255)                                    aCAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctc                                                                                                                                                                                                                <  1:171549‑M1/250‑15 (MQ=255)                                    aCAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctc                                                                                                                                                                                                                <  1:409348‑M1/250‑15 (MQ=255)                                        gaagCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcacttt                                                                                                                                                                                                           <  2:99053‑M1/251‑20 (MQ=255)                                          agCCAGCGATCGCGGTATTGTGGTGGTCAACATGACACCATGTATGTACGGTAAAGTGAACCTGGGTGGTTATGCCACCGGTAACGCCCTCGACCATGCCGGCGTAATTGGCGGTGGAGATATGCCTTTAGAAGCCAAGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGCTAATGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCTGCGCACTGACGCtttgctcccatctcactttac                                                                                                                                                                                                         >  2:29919‑M1/1‑230 (MQ=255)                                            ccAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatc                                                                                                                                                                                                       >  2:545077‑M1/1‑228 (MQ=255)                                             cAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcg                                                                                                                                                                                                      <  1:545077‑M1/251‑25 (MQ=255)                                                cGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCCGGAACTGGATACTGAAACCATTCGCAAGGCCCTGAGCTAAAACCGGCGCGGCGACCTGTCGCtttgctcacctctcactttaatcgtgc                                                                                                                                                                                                   >  2:29965‑M1/1‑224 (MQ=255)                                                      cgGTATTGGGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacat                                                                                                                                                                                             <  2:506272‑M1/251‑34 (MQ=255)                                                       ggTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGGTAATGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacatt                                                                                                                                                                                            >  1:348110‑M1/1‑217 (MQ=255)                                                       ggTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacatt                                                                                                                                                                                            <  1:248004‑M1/251‑35 (MQ=255)                                                       ggTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcctgctcacatt                                                                                                                                                                                            >  1:409956‑M1/1‑217 (MQ=255)                                                          aTTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTTAAAACATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgttctcacattacg                                                                                                                                                                                         >  2:731434‑M1/1‑214 (MQ=255)                                                            tgtgGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGACCATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgcccacatctcactttcatcgtgctcacattacgt                                                                                                                                                                                        <  2:629156‑M1/250‑39 (MQ=255)                                                                     cAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattc                                                                                                                                                                               <  1:224204‑M1/250‑48 (MQ=255)                                                                             cacaATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAAACATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaacc                                                                                                                                                                      >  2:186373‑M1/1‑195 (MQ=255)                                                                                     atgtCCGTGAAAGGGATCATGGGGGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCTTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTTCCTGCTTAGCCAGGATCTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGATGCtttgctctcatctcacgttaaacgtgctcacattacgtgacggattctaacaaaacactaacac                                                                                                                                                              <  2:504996‑M1/251‑65 (MQ=255)                                                                                     atgtCCGGTAAAGTGAACATGGGTGGTTCTGCCACCGGTAACGCCCTCGCCACTGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCTCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacatgacgtgactgactctaacaaaacattaaca                                                                                                                                                               <  2:638240‑M1/250‑64 (MQ=255)                                                                                     atgtCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCCTGCCGGCGTAATTGGCGGTGCAGATATAACTGTAGCAGCCACGCTAACCAAACTGCATTACCTGCTTCGCCAGGAAATGGATACTGAAACCATTCGCAAGGCCATGCGCTCAAACCTGCGCGGCGAACTGACGCtttgctcacacctcactttaatcttgctcacattacgtgccttattcttcccaacccttacccc                                                                                                                                                              >  2:273721‑M1/1‑187 (MQ=255)                                                                                         ccGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGTCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctctctttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaac                                                                                                                                                          <  2:454083‑M1/251‑69 (MQ=255)                                                                                           ggTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactg                                                                                                                                                        >  1:682190‑M1/1‑181 (MQ=255)                                                                                              aaaGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggc                                                                                                                                                      >  1:719674‑M1/1‑178 (MQ=255)                                                                                                      cATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttg                                                                                                                                             <  1:273721‑M1/251‑82 (MQ=255)                                                                                                       aTGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgt                                                                                                                                            >  1:514487‑M1/1‑169 (MQ=255)                                                                                                          ggtggtTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCCTTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaanacattaacaccaactggcaaaattttttcct                                                                                                                                         >  2:335086‑M1/1‑166 (MQ=255)                                                                                                            gggttATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTTTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaa                                                                                                                                       <  2:527011‑M1/250‑88 (MQ=255)                                                                                                            gggttATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGGGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccacctggcaaaattttgtcctaa                                                                                                                                       <  2:331370‑M1/250‑88 (MQ=255)                                                                                                                 aTGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaactt                                                                                                                                   <  2:551715‑M1/250‑92 (MQ=255)                                                                                                                   gCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgat                                                                                                                                >  1:146080‑M1/1‑157 (MQ=255)                                                                                                                          gTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacg                                                                                                                         >  1:224284‑M1/1‑150 (MQ=255)                                                                                                                             aCGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaa                                                                                                                       >  1:343949‑M1/1‑147 (MQ=255)                                                                                                                                 ccTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggc                                                                                                                  <  2:37820‑M1/251‑109 (MQ=255)                                                                                                                                      cccATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgtcgaaatggctgcac                                                                                                             <  1:29919‑M1/251‑114 (MQ=255)                                                                                                                                         aggccGGCGTAATTGGCGGTGCAGATATGACTGGAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcaccta                                                                                                          <  2:224284‑M1/249‑117 (MQ=255)                                                                                                                                             cGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatc                                                                                                      <  1:186174‑M1/251‑121 (MQ=255)                                                                                                                                              ggCGTAAGTGGCGGTGCAGATATGACTTTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATGCGCGAGGCCATGAGCTAAAACCTTCGCGGGGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaagcttgatctcgacgaaatggctgctcctaaatcg                                                                                                     <  2:271752‑M1/251‑122 (MQ=255)                                                                                                                                                   aaTTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatg                                                                                                <  1:499972‑M1/251‑127 (MQ=255)                                                                                                                                                       ggCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaa                                                                                             <  1:349350‑M1/250‑130 (MQ=255)                                                                                                                                                         cGGTGCAGATATGACTGTAGAAGACACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGTTAAAACCTGCGCGGCGATCTGACGCtttgctcacatctcactttaagcgtgctcacagtacgtgactgattctaacaaaacattaacaccaactggcaaaatgttgtcctaaacttgatctcggcgaaatggctgcggctacatcgggatgaaaatc                                                                                          <  2:504504‑M1/251‑133 (MQ=255)                                                                                                                                                          ggTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatc                                                                                          >  1:220950‑M1/1‑118 (MQ=255)                                                                                                                                                              cAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgctgaaaatcacatt                                                                                     >  2:604280‑M1/1‑114 (MQ=255)                                                                                                                                                                     gACTGTAGAAGCCACGCTAACCAAACTGCATTTCCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcg                                                                              <  2:514487‑M1/251‑145 (MQ=255)                                                                                                                                                                     gACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcg                                                                              >  1:482336‑M1/1‑107 (MQ=255)                                                                                                                                                                      aCTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgt                                                                             <  2:682190‑M1/251‑146 (MQ=255)                                                                                                                                                                       cTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgt                                                                             >  2:164274‑M1/1‑105 (MQ=255)                                                                                                                                                                         gTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACAGGATACTGAACCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcacttcaatagtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctcaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaat                                                                          >  1:651608‑M1/1‑103 (MQ=255)                                                                                                                                                                           aGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattg                                                                        >  2:190262‑M1/1‑101 (MQ=255)                                                                                                                                                                           aGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattg                                                                        >  1:657541‑M1/1‑101 (MQ=255)                                                                                                                                                                             aaGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcc                                                                      <  1:186373‑M1/251‑153 (MQ=255)                                                                                                                                                                               gCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccct                                                                    <  1:590509‑M1/251‑155 (MQ=255)                                                                                                                                                                                 cACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcccttt                                                                  >  2:20516‑M1/1‑95 (MQ=255)                                                                                                                                                                                  ccgCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcccttta                                                                 <  1:164274‑M1/250‑158 (MQ=255)                                                                                                                                                                                   cGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcccttta                                                                 >  2:312677‑M1/1‑93 (MQ=255)                                                                                                                                                                                    gCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaa                                                               <  1:731434‑M1/251‑160 (MQ=255)                                                                                                                                                                                     cTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcccttta                                                                 <  2:102622‑M1/248‑158 (MQ=255)                                                                                                                                                                                     cTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgcccttta                                                                 >  1:102622‑M1/1‑91 (MQ=255)                                                                                                                                                                                        ggcaaACTGCATTACCTGCTTAGCCAGGAACTGGATACTTAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTTACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaat                                                             <  2:484689‑M1/247‑162 (MQ=255)                                                                                                                                                                                           aaaCTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggg                                                        >  1:7447‑M1/1‑85 (MQ=255)                                                                                                                                                                                                gCATTGCCTGCTTAGCCAGTAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgcc                                                   <  1:491848‑M1/251‑172 (MQ=255)                                                                                                                                                                                                          cTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccacctggcaaaattttgtcctaaccttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgt                                         >  2:564387‑M1/1‑70 (MQ=255)                                                                                                                                                                                                                     aCTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttttcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcc                              <  1:475370‑M1/251‑193 (MQ=255)                                                                                                                                                                                                                        ggATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgcctgattctaacaaaacattaacaccaactggcaaaattttgtcctacacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtagtctcaagccca                            >  2:74990‑M1/1‑56 (MQ=255)                                                                                                                                                                                                                                aaaCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGAAGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattaggggcgccgaccccatgtggtctcaagcccaaaggaag                     >  2:505944‑M1/1‑48 (MQ=255)                                                                                                                                                                                                                                        cGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagtgaggcga           <  1:335086‑M1/251‑212 (MQ=255)                                                                                                                                                                                                                                          cAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatcgcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtcgcaagcccaaaggaagagtgaggcgagt         <  1:74990‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                                                 aTGAGCTAAAACCTGCGCTGCGAACTTACGCtttgctcacatctccctttaatcgtgctcacattacgtgactgattctaacaaaacattaactccaactggcaaaatgttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttgtatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaaggaagagttaggcgtgtcagtcgc  <  2:482336‑M1/251‑221 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTG  >  NC_000913/1851595‑1852085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑