Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,917	C→T	Q246Y (CAG→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,917	0	C	T	100.0%	241.1 / NA	68	Q246Y (CAG→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/39);  total (29/39)

TGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACC  >  NC_000913/1301676‑1302154                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                tgatgaTCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCt                                                                                                                                                                                                                                        >  2:576085/1‑251 (MQ=255)   atgatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTAt                                                                                                                                                                                                                                      <  2:183968/251‑1 (MQ=255)     gatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                                                                                                                                                                                                    <  1:6736/251‑1 (MQ=255)          cacaTTAGAAGGCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                                                                                                                                                                                               <  2:706166/251‑1 (MQ=255)           acaTTAGAAGTCACCTTAAGTGAACCCGTTCNGTACTTCTATAAATTACTTGTTCCCCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                                                                                                                                                                                              <  2:368994/251‑1 (MQ=255)           acaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAAGTACTTGTTCACCCAGCATCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                                                                                                                                                                                              <  2:703527/251‑1 (MQ=255)                  aaGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                                                                                                                                                                                                       >  1:472995/1‑251 (MQ=255)                        ccTTTAGTGAACCCGTTCCGTACGTCTAGAAATTACTTTTTCACCCATCAACTTCTCCGGTGCCAAAAGCCTCTATCGATAAATTCGGCGAAAAATGGACCCAGCTTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAGTGGGTCGTAAACGAACGAAGCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAGTGCTTCTGaa                                                                                                                                                                                                                    <  2:185047/248‑1 (MQ=255)                        ccTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTTTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                                                                                                                                                                                                 <  1:674541/251‑1 (MQ=255)                        ccTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                                                                                                                                                                                                 >  1:468135/1‑251 (MQ=255)                              gTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGAt                                                                                                                                                                                                          >  1:567940/1‑251 (MQ=255)                                     cgttccgttcTTCTATAAATTACTTGTTCACCCATAAACTTCACCGGTGCCAAAAGCCGCTATCGAAAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTATAAGATTGGGTCGTAAACGAACGAATCTTACTTGAACGCAGCCCGACCTACTGTAAC‑GACGCGAAGACCTTTATTACCTATGTATCCTATTTGGCTATTGCTTCTGAAGTTA‑CCGATGTCatcc                                                                                                                                                                                                   <  2:481128/251‑4 (MQ=255)                                         ccGTACTTCTATAAATTACTTGGTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCt                                                                                                                                                                                                <  1:39169/250‑1 (MQ=255)                                             atttCTATAAATTACTTGTTCACCCATCAACTTCCCCGGTGACAAAAGCAGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTACACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATGGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGt                                                                                                                                                                                           <  2:588329/249‑1 (MQ=255)                                                   ataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAgtgg                                                                                                                                                                                      <  1:576085/250‑1 (MQ=255)                                                      aaTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGaa                                                                                                                                                                                  <  2:567940/251‑1 (MQ=255)                                                        ttACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCACTACCGTAGTGGTGAAAt                                                                                                                                                                                >  1:209279/1‑251 (MQ=255)                                                          aCTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCg                                                                                                                                                                              >  1:748187/1‑251 (MQ=255)                                                           cTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCg                                                                                                                                                                              >  1:740722/1‑250 (MQ=255)                                                                 cACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                                                                                                                                                                                                        >  1:150464/1‑203 (MQ=255)                                                                 cACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                                                                                                                                                                                                        <  2:150464/203‑1 (MQ=255)                                                                 cACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCGACCGTAGTGGTGAAATCGACATGAc                                                                                                                                                                       <  2:495565/251‑1 (MQ=255)                                                                  aCCCATCAACTTCACCTGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTATCCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACg                                                                                                                                                                      <  2:740722/251‑1 (MQ=255)                                                                          aCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAAc                                                                                                                                                                >  1:46462/1‑249 (MQ=255)                                                                                ccggtttcAAAAGCCGCTATCGAGAAATTCGGCGAAAAAAGGACCCAGCCTGGTAATAGCGTCCCCAACGGTGCCTATACCTTAAAAGAGTGGGTCGTGAACGAACGAATCGTTCTTTAACGCACCCCGACCTCCTTGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTTCTTCTGAAGTTC‑CCGATGTCACCCGCTACCGTAGTGGTGAAATCTACATTCCGAATAACAGCATGcc                                                                                                                                                        <  2:1181/244‑1 (MQ=255)                                                                                    ggCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATc                                                                                                                                                    <  2:75182/250‑1 (MQ=255)                                                                                     tcaaaaaGCAGCTATCGAGAAATGCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCCACAACGGTGCCTATAACTTAAAAGATTGTGTTGGAAACGTGCCAATCTTGTTTGAACGCCGACCGCCCTACTGGATC‑ACCGCGAAAACCGTTCGTAACTATGTAACCTATTTGCCTATTGCTTCTAAAGTTA‑CAGATGTCACCCGCTACCGAAGTGGTGAAATCGACATGACGTATAACAGccgacctttc                                                                                                                                                    <  2:221416/247‑10 (MQ=255)                                                                                     gCCAAAAGCCGCTATCGAGAAAGTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCg                                                                                                                                                   <  2:119459/251‑1 (MQ=255)                                                                                          aaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAAttt                                                                                                                                              >  1:174957/1‑250 (MQ=255)                                                                                              cGCTAGCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTTAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTcc                                                                                                                                          <  2:598870/251‑1 (MQ=255)                                                                                               gCTATCGAGAAATTCGGCGAAAAACGGACCCAGCCTGGCAATATCGTCACACACCGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTC‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGACAAACAGCATGCCGCTCGAATTGTTcc                                                                                                                                          >  1:290530/1‑250 (MQ=255)                                                                                                    cGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGc                                                                                                                                    <  1:608979/251‑1 (MQ=255)                                                                                                            tCGGCGAAAAATGGACCCAGCCTGGTAATANCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                             <  2:341288/250‑1 (MQ=255)                                                                                                             cGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                             >  1:639302/1‑249 (MQ=255)                                                                                                                 gAAAAATGGACCCAGCCGGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAAGAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                                                                                       <  2:748187/251‑1 (MQ=255)                                                                                                                    aaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                                                                                                    <  2:233696/251‑1 (MQ=255)                                                                                                                     aaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                   <  2:516835/251‑1 (MQ=255)                                                                                                                        ggACCCAGCCTGGTAATATCTTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGAc                                                                                                                <  2:468135/251‑1 (MQ=255)                                                                                                                                        tatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATc                                                                                                >  1:551672/1‑251 (MQ=255)                                                                                                                                         atCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATcc                                                                                               >  2:264136/1‑251 (MQ=255)                                                                                                                                           cGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTAATGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                             >  1:658649/1‑251 (MQ=255)                                                                                                                                             tCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                           >  2:128425/1‑251 (MQ=255)                                                                                                                                              cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                          <  2:46462/251‑1 (MQ=255)                                                                                                                                                     ggTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAc                                                                                  >  1:718648/1‑251 (MQ=255)                                                                                                                                                     ggTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAc                                                                                  >  1:74854/1‑251 (MQ=255)                                                                                                                                                          cTATACCTTAACACACTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGCCCTCCTGGAACCAAC‑CGAAAACCGTCATTCACTATGTAACCTATTTGCCTATTCCTTCCGAAGTTA‑CCGCTGTAAACCGCTACCTTAGTGGTGCAATCGACATGACGAATAACAGCATGCCGGTCGAATTGTTCCAGAAGCTGAAACAAGAGATCCCGGACGCAGTTCACGTTGATCACTACCTGTG‑CACTTACt                                                                             >  2:694441/1‑251 (MQ=255)                                                                                                                                                               ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTAc                                                                        <  2:248928/251‑1 (MQ=255)                                                                                                                                                                  tAAAAGATTGGGTCGTTAACGAACGCATCGTGCTTGAACGCAGCCCGACCTCCTGGAAC‑AACGCCAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTCCCGTAGTGGTGAAATCAACCTGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTTCGAAGTTCTCGTTGATCCTTACCTGTG‑CACTTACTATTACGa                                                                      <  1:86768/250‑1 (MQ=255)                                                                                                                                                                     aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACTGACTGGAAC‑AACGCGAAATCCGTTATTAACTATGTAACCTATTTGCTTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGACCGAATTGTTTCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGGTGATCCATCCCTGTG‑CACCTATTATTACGAAAtt                                                                  <  2:632644/251‑1 (MQ=255)                                                                                                                                                                     aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAAtt                                                                  <  2:643622/251‑1 (MQ=255)                                                                                                                                                                        aTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTaac                                                               <  2:316965/251‑1 (MQ=255)                                                                                                                                                                        aTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTaac                                                               >  1:74188/1‑251 (MQ=255)                                                                                                                                                                                  aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAAc                                                     >  1:26203/1‑251 (MQ=255)                                                                                                                                                                                   aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAGCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAAcc                                                    <  2:73812/251‑1 (MQ=255)                                                                                                                                                                                        acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTTCAGAAGCTGAAACAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTCCTATTACGAAATTAACAACCAGAAACCGCCAt                                               >  1:246828/1‑251 (MQ=255)                                                                                                                                                                                          gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTTTTACg                                                                       >  2:670393/1‑225 (MQ=255)                                                                                                                                                                                          gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACg                                                                       <  1:670393/225‑1 (MQ=255)                                                                                                                                                                                          aaaTCGTGCTTGAACGCAGCCCCACCGACGGGAAA‑AAGGCAAAAACCGTTATTAACTAGGTAACCTATTTGCATATTGCTTCGGAAGTTA‑CCGATGTCATCCGCTGCCGTAGTGGTGAAATCGACATGGCGAATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAATTTCACGTGGATCCATGCC‑GTGGCACTTACTATTGCGAAATTAACAACCAGAAACCGCCATTc                                             <  2:459506/250‑1 (MQ=255)                                                                                                                                                                                              cGTTCTTGAACGCAGCCCGACCTACTGGAAA‑NACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAACCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                         <  2:369817/251‑1 (MQ=255)                                                                                                                                                                                               gTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTAAAATCGACATGACGAATAACAGCATGACGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAAACAGAAACCGCCATTCAACg                                         >  1:494859/1‑250 (MQ=255)                                                                                                                                                                                                  ctatatcGCAGCACGCCCTACTGGAAT‑AACGCTAAATCCCTCATTAACTATGCAACCTATTTGCCTATTACTTCTGAAGTTA‑CCAATGTTAACCGCTACCGTAGTGGTGAAATGGACGTGTCTTATAACAGTATGCCGATCGAATTGTTCCAGAACATGAATGCAGAGTTCCCGGAAGAAGTTCACGTTAATCCATACCTGTG‑CACTTACTATTACGAAATTTCAAACAAGAAACCGcaagtccacggtgt                                     >  2:429186/7‑238 (MQ=255)                                                                                                                                                                                                    tGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgt                                     <  1:31723/249‑1 (MQ=255)                                                                                                                                                                                                           aGCCCGACCTACTGGAAA‑AACGCGAAAACCTTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATAACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                            <  2:74854/251‑1 (MQ=255)                                                                                                                                                                                                            gCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTCTTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcttgtgctt                           >  2:684104/1‑249 (MQ=255)                                                                                                                                                                                                                          aac‑aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATCACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt             >  1:678832/1‑251 (MQ=255)                                                                                                                                                                                                                                 gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt       >  1:569262/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCAGATCGAATTGTTCCAGAAGCTGAAACAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGACACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGCATGGAc   >  1:286686/1‑251 (MQ=255)                                                                                                                                                                                                                                      acccGTTTTTAACTATGTCACCTATTTGCTTATTGCTTCTGAA‑TTATCCGAGGTCAACCGCTACCGTAGTGTTGAAATCGACATGACGAAAAACGGCATCCCGTGCGAATTGTTCCAGAAGCGGAAAAAAGAGATTCCGGACGAAGTCCACGTTGATCCATACCTGTG‑CCTTTACTATTACAAAATAAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATggtcc  <  2:498705/249‑4 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                TGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAAC‑AACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTA‑CCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACC  >  NC_000913/1301676‑1302154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑