Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,861	A→G	intergenic (+79/+6)	C0293 → / ← ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,861	0	A	G	100.0%	117.1 / NA	37	intergenic (+79/+6)	C0293/ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

ATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTC  >  NC_000913/1196630‑1197102                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aTTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgatta                                                                                                                                                                                                                                 >  1:471350‑M1/1‑237 (MQ=255)           attaGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttc                                                                                                                                                                                                                      <  2:623341‑M1/251‑25 (MQ=255)               ggACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcg                                                                                                                                                                                                                  <  1:248875‑M1/251‑29 (MQ=255)                   aaTTATGAATTACCACTCCTTACACCTGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagt                                                                                                                                                                                                              <  1:251156‑M1/251‑33 (MQ=255)                       aTGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagtttt                                                                                                                                                                                                           <  2:548182‑M1/250‑36 (MQ=255)                         gAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgt                                                                                                                                                                                                        >  2:266963‑M1/1‑213 (MQ=255)                           aTTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCCCCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCTGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgttt                                                                                                                                                                                                      <  2:495948‑M1/251‑41 (MQ=255)                            ttACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgttt                                                                                                                                                                                                      >  2:264330‑M1/1‑210 (MQ=255)                                 aCTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCACCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccgtcaacgattaaactgcgcttcggcgcagttttcgtttacagg                                                                                                                                                                                                 >  1:379398‑M1/1‑205 (MQ=255)                                               cTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaa                                                                                                                                                                                   >  2:700505‑M1/1‑191 (MQ=255)                                                    atatTGTTAAAGTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattct                                                                                                                                                                             <  2:21849‑M1/251‑66 (MQ=255)                                                      atttttaaatTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctgg                                                                                                                                                                           <  1:126177‑M1/247‑68 (MQ=255)                                                      atTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACCGACAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcacagattaaactgcgcttcggcgcaattttcgtttacaggatgttgaaagggaaaattatgg                                                                                                                                                                           >  1:336018‑M1/1‑184 (MQ=255)                                                      atTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctgg                                                                                                                                                                           >  1:439775‑M1/1‑184 (MQ=255)                                                            aaaTTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaa                                                                                                                                                                      >  1:371154‑M1/1‑178 (MQ=255)                                                                    ggTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgacagggaaaattctggggcaaaaaaagccc                                                                                                                                                             >  2:559269‑M1/1‑170 (MQ=255)                                                                        ttGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaacattctggggcaaaaaaagcccgcc                                                                                                                                                          >  1:432780‑M1/1‑166 (MQ=255)                                                                        ttGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgcc                                                                                                                                                          >  1:165563‑M1/1‑166 (MQ=255)                                                                         tGTATCAACTACTCACCCGGGCCGCGCCAGGGGACAGCAAACAGGGATTGGGTGCAAGCACCTTAGCTTTTAGGTACATGCGGAATGGAAAAAAGGCCGCGAGCGCTGTCCCTACCCATACATCTTTAGTACTGAGACTGTTTAATCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcaggtttcgtttacaggatgtggaaagggaaaattctggggcaaaaaaagcccgcca                                                                                                                                                         <  2:167007‑M1/250‑86 (MQ=255)                                                                           tATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCACCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagtt                                                                                                                                                      >  1:327119‑M1/1‑163 (MQ=255)                                                                                          cGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggcaaaccc                                                                                                                                       >  1:583364‑M1/1‑148 (MQ=255)                                                                                             gACTCGCCAGGGGACAGCCAACAGGCATTGGGTGAAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAACAAGGCCGCGAGCGCGGCCCCTTCACATACATATTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacgtagggagaactcat                                                                                                                                     >  2:571052‑M1/1‑145 (MQ=255)                                                                                                     aGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaaacccaccccatgggg                                                                                                                            >  1:645591‑M1/1‑137 (MQ=255)                                                                                                         gACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGTTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGTCTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaac                                                                                                                        <  2:198729‑M1/251‑119 (MQ=255)                                                                                                         gACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAAGGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaac                                                                                                                        <  1:323444‑M1/251‑119 (MQ=255)                                                                                                             gCCAACAGGCATTGGGGGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCTCATACATCTTTAGGACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgctgcggcgcagttttcgtttacaggatgttgaaagggaaaattcttgggtaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatg                                                                                                                    <  1:34941‑M1/251‑123 (MQ=255)                                                                                                                    ggCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatg                                                                                                             <  1:401290‑M1/251‑130 (MQ=255)                                                                                                                                   cACCTTAGCGTTCAGGTACAGGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTCccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttat                                                                                              <  2:471350‑M1/251‑145 (MQ=255)                                                                                                                                        tAGCGTCCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcat                                                                                           <  2:264211‑M1/249‑148 (MQ=255)                                                                                                                                                    aCATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaaccccctcccattggtgaacaatgaataaagaaaattcgggggtattatctcccagcatattc                                                                             >  1:742638‑M1/1‑90 (MQ=255)                                                                                                                                                      aTGCGGAATGTAAAAAAGGCCGCGAGCGCGGNCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccat                                                                           <  2:371154‑M1/251‑164 (MQ=255)                                                                                                                                                                          cgcgAGCGCGGCCCCTTCACATACATCTTTANTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttattta                                                       <  2:344835‑M1/251‑184 (MQ=255)                                                                                                                                                                                   ggCCCCTTCACATTCATCTTTAGTACTGAGACTTTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggtgttatcatctcccagtatatccatactaacaataaggttatttactcaaccag                                              <  1:700505‑M1/251‑193 (MQ=255)                                                                                                                                                                                    gCCCTTTCACATACATCTTTAGTACTGAGACNGTTTAACCTAGGGAATTTTTGTCGTCccagcaacgataaaccggagcttcggcgcagttttcggttacaggatgttgaaagggaaaattttggggcaaaaaaagaccgccagttacgtcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttagttactcaaccagg                                             <  2:379398‑M1/251‑194 (MQ=255)                                                                                                                                                                                                      ttAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgtttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttt                             <  2:645591‑M1/249‑210 (MQ=255)                                                                                                                                                                                                                          ccTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttttgtgcgtgggaacagccttaag       <  1:738131‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                               ggAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacgggatgttgaaagggaaaattctggggcaaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttttgtgcgtgggaacagccttaaggtgt  <  1:288759‑M1/251‑237 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTC  >  NC_000913/1196630‑1197102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑