Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,289	A→G	Y235H (TAT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,289	0	A	G	100.0%	216.6 / NA	60	Y235H (TAT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/24);  total (36/24)

TGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAG  >  NC_000913/4312048‑4312527                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               ttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGt                                                                                                                                                                                                                                      >  1:539105‑M2/10‑251 (MQ=255)    acgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTc                                                                                                                                                                                                                                   <  1:4869‑M2/245‑1 (MQ=255)    acgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTc                                                                                                                                                                                                                                   >  2:4869‑M2/7‑251 (MQ=255)      gtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGc                                                                                                                                                                                                                                 >  1:7793‑M2/5‑251 (MQ=255)                      agTAACTCCGGGATGGCTGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTAAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGAGCGGCCAGTTTCGCTATCGGTGAATGGTa                                                                                                                                                                                                                  <  2:275410/250‑1 (MQ=255)                                 gttGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACATAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTAGCGGTGAATGGTAGCTATGGGTta                                                                                                                                                                                                       <  1:454001/249‑1 (MQ=255)                                  aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTtat                                                                                                                                                                                                      <  1:38813/251‑1 (MQ=255)                                   tGGCGGCATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTtat                                                                                                                                                                                                      >  2:318328/1‑250 (MQ=255)                                      cGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACaa                                                                                                                                                                                                  <  2:24883/251‑1 (MQ=255)                                       ggAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACaa                                                                                                                                                                                                  >  1:491912/1‑250 (MQ=255)                                       ggAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGACAGTTTCGCTATCGGGGAATGGTAGCTATGGGTTATACaa                                                                                                                                                                                                  >  1:576776/1‑250 (MQ=255)                                             aCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATActct                                                                                                                                                                                           >  1:389677/1‑251 (MQ=255)                                                   ttCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGCTTCGCTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGccc                                                                                                                                                                                     >  2:400031/1‑251 (MQ=255)                                                        aTTTCAGCGCGCCTGGTGATAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTGCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAATGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAAGATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTTTGGGTTATACAAATAATCTTTGCCCCTTTc                                                                                                                                                                                <  2:464801/251‑1 (MQ=255)                                                        aTTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGGGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGAAGCTATTGGGTATTCAAATAATCTTTGcccatttc                                                                                                                                                                                >  2:414327/1‑246 (MQ=255)                                                                 cgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGcc                                                                                                                                                                       <  1:649433/251‑1 (MQ=255)                                                                 cgcCTGGTGAGAAGAAATCAACAATTGCGACGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGcc                                                                                                                                                                       >  1:749784/1‑251 (MQ=255)                                                                               aaaTCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGc                                                                                                                                                         >  1:402709/1‑251 (MQ=255)                                                                               aaaTCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGc                                                                                                                                                         >  2:429844/1‑251 (MQ=255)                                                                                 aTCAACAATTGCGCCGGTTTTTTCCATATTATTATTAGCTTGTTCAATTTTGAGCTGGGCGACAGAGACAAAAAAAGCTTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGCCCTAATAACGGGGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCAAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATTTTTTCCCCGTTCTTTTTTGCAATTTCTACGGCCGCtt                                                                                                                                                       >  2:111037/1‑251 (MQ=255)                                                                                 aTCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGATACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAACTAATATGATCGGCCAGTTTCGCTATCGGTGAATGGCAGCTATGGGTTATACAAATAATATTTCCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCtt                                                                                                                                                       >  2:503238/1‑251 (MQ=255)                                                                                  acaacaaTTGCGCCGGTTTTATCCATATTAATAGTAGATTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGACCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATCCAAATTATCTTTGCCCCTTTCTTTTTTGCTAGTTCTACGGCCGCttt                                                                                                                                                      <  1:221581/250‑1 (MQ=255)                                                                                                 gTTTTTTCCATATTAATATCAGCTTGTTCAATGTTGAGCTGGGCGACAGAGCCAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATCATATGGTCGGCCAGTTTCGCTATCGGTGCATGGTAGCTATGGGTTATACCACTAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGtttc                                                                                                                                       >  1:363981/1‑249 (MQ=255)                                                                                                  ttttttCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGc                                                                                                                                      >  2:757156/1‑251 (MQ=255)                                                                                                     tttccATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGccc                                                                                                                                    <  1:757156/246‑1 (MQ=255)                                                                                                            attaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGGAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGACTggg                                                                                                                            >  2:197925/1‑251 (MQ=255)                                                                                                                     aGCTTGTTCAATGGTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAAAATTCTTCCCGAGGCATTCCGCCCTAATAACGGCGTTTCCGGGGCTGGTAAGCAAATAATAAGATCGGCCAGTTTCGCAATAGGGGAATGGAAGCTTTGGGTTATCCAAATAATCTTTGCCCCGTTCTTTTTTGCCGGTTCTACGCCCGCTTTTCCATCCCTGGTTCACCCGGATTgggcccctaca                                                                                                                    >  1:42921/1‑242 (MQ=255)                                                                                                                         tGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGcac                                                                                                               >  1:680815/1‑251 (MQ=255)                                                                                                                              aaTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAt                                                                                                          <  1:705413/251‑1 (MQ=255)                                                                                                                               aTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCAACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtc                                                                                                         <  1:616287/251‑1 (MQ=255)                                                                                                                                     aGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttc                                                                                                   <  1:318328/251‑1 (MQ=255)                                                                                                                                         gggCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttcct                                                                                                 <  2:7793/249‑1 (MQ=255)                                                                                                                                              aCAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaacaa                                                                                          <  1:558353/251‑1 (MQ=255)                                                                                                                                                 gagaCAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGa                                                                                       <  1:400031/251‑1 (MQ=255)                                                                                                                                                         aaaaGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGCGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGCATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCggcc                                                                                >  2:294221/1‑248 (MQ=255)                                                                                                                                                               gTCCAGCAAGGTTGGGTGAAATATTCTTGCCGAGGCATTACGACCTCATAACGGCGTTTCCGGGGCTGGTGAGCACATAATATGATCAGCCAGTTTCGCTATATGTGCATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTATTTTTTGCCAGTTCTACAGCCGCTTTTACATAACTGGGTCGCCCGGGATGGGTCACTACCAGCCCAACACCTCCCTCATGTAACAACGAAGCGGAcatcatc                                                                          >  2:594848/1‑250 (MQ=255)                                                                                                                                                                tCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatcat                                                                        >  1:343599/1‑251 (MQ=255)                                                                                                                                                                     gaaggttggttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCGGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCGCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgt                                                                   <  1:645680/243‑1 (MQ=255)                                                                                                                                                                      aaggttggttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgtg                                                                  >  1:371734/8‑251 (MQ=255)                                                                                                                                                                      aaggttggttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGAtgtg                                                                  >  1:26818/8‑251 (MQ=255)                                                                                                                                                                           tggttGTAATATTCTTGCCGAGGCATTACGCCCTAATAACGGCGTTTACGGGGCTGGTGAGCAAATAATATGCTCGGCCAGTTTCGGCATCGGTGAATGGTAGCTAAGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTCCGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCCCTACCAGAACAACATCTCCTTCCTGTAAAAACGAAGCGGACCTCATCATGCTGCGAGCAt                                                             >  2:328128/3‑251 (MQ=255)                                                                                                                                                                            ggttGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATc                                                            <  1:202942/250‑1 (MQ=255)                                                                                                                                                                                gttatatTCTTGCCGGGGTTGTACGGCATAATAACGGTGTTTCAGGGGCTGGTGAACAAATAAGATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATTGTTTATACAAATAATCATTGCCCCGATCTTTTTGGCCAGTTCTACGGCCGCTGTTACATCACTGTTTCGCCCTTAATGGGTCACTACCAGCACAACATCTCCTCCCTGGAACAACGAAGCGGCCATCATCAGGATGTGATCTTCAGGa                                                        <  2:42921/248‑1 (MQ=255)                                                                                                                                                                                   attttCTGGCCGAGTCATTACGACCTAATAACGGCGTTTCCGGGGCTGGGGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCCCTGGTTCGCCCGGAATGGGGCACTACCAGCACAACAGCTCCTGCCGGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa                                                      <  2:539105/247‑1 (MQ=255)                                                                                                                                                                                            ccGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCa                                                                                                                   >  1:222637/1‑180 (MQ=255)                                                                                                                                                                                            ccGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTGTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCa                                                                                                                   <  2:222637/180‑1 (MQ=255)                                                                                                                                                                                             cGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATATTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGc                                           >  1:267200/1‑251 (MQ=255)                                                                                                                                                                                                   aTTACGTCCTAACAACGGCGTTTCCGGGGCTGGTGTGCAAATAATATGATCGGCCAGTTTCGCTATCGGTTAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCTTTCTTTTTTGCCAGTTCTACGGCTGCTTTTACATCACTGGTGCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAATCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGc                                     <  2:679141/251‑1 (MQ=255)                                                                                                                                                                                                    ttACGACCTAATAACGGCGTTTCCGGTGCTGGTGAGCAAATAATATGATCGGCAAGGTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAAGGCAGCTTTTACATCACTGGTTCGCCCGGAATGGGGAACTACCAGCACAACATCTCCTTCATGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGACTGACAGCGtccacc                                    >  2:423843/1‑246 (MQ=255)                                                                                                                                                                                                                aaCGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGa                         >  1:445655/1‑250 (MQ=255)                                                                                                                                                                                                                  cGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGATTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACt                      >  1:509530/1‑251 (MQ=255)                                                                                                                                                                                                                   ggCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACtt                     >  2:6149/1‑251 (MQ=255)                                                                                                                                                                                                                      gTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTtgtg                  >  1:261100/1‑251 (MQ=255)                                                                                                                                                                                                                         tCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCCCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATACCCTTCAGGTAACAACGAAGCGGACATCATCCTGATGTGAGCATCAGGATAGGCCTGACAGCGTACACCAATGGGAAAGACCTtgctatg               >  2:71519/1‑246 (MQ=255)                                                                                                                                                                                                                          ccGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGt              >  1:18003/1‑251 (MQ=255)                                                                                                                                                                                                                           cGGGGCTGGGGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGCTCTACGGCCGCTTTTACATCACTGGCTCGCCCGGACTGGGTCACTACCCGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCACGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGt              >  2:699173/1‑250 (MQ=255)                                                                                                                                                                                                                               gCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATc         >  1:600308/1‑251 (MQ=255)                                                                                                                                                                                                                                     gAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcaca   <  1:414327/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcac    <  1:429844/250‑1 (MQ=255)                                                                                                                                                                                                                                      aGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAATCGGACATCATCATGAGGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAg  <  2:491912/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               TGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAG  >  NC_000913/4312048‑4312527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑