BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,811	G→A	intergenic (+1254/‑390)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,811	0	G	A	96.6%	387.0 / NA	118	intergenic (+1254/‑390)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (39/74); minor base C (4/0); total (44/74)
Fisher's exact test for biased strand distribution p-value = 1.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCACAAGTCGCGCGACCGAAC‑GATACCGTTGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCG  >  NC_000913/3951566‑3952056

  gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GAt                                                                                                                                                                                                                                                      >  1:324719/1‑250 (MQ=255)

            catgccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGATAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGACAGGAAAATTTCATCAACTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCCCGCCACCAAAC‑GAAACCGtttct                                                                                                                                                                                                                                             >  1:361831/5‑246 (MQ=255)

                 cTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTAtct                                                                                                                                                                                                                                      >  1:128075/1‑251 (MQ=255)

                   ggCGTTACGACCATCCCGGTGACGCTAGCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTAtct                                                                                                                                                                                                                                      <  1:121960/249‑1 (MQ=255)

                      gTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑ggg                                                                                                                                                                                                                                >  1:25878/1‑250 (MQ=255)

                      gTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGt                                                                                                                                                                                                                                <  1:602588/251‑1 (MQ=255)

                       ttACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GGTACCGTCGTCTGTATCTCC‑gggg                                                                                                                                                                                                                               >  1:32431/1‑249 (MQ=255)

                            cccATCCCGGTGACGCGAGCTACGCGCCGTTGTTGTGAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGc                                                                                                                                                                                                                          <  2:712219/250‑1 (MQ=255)

                                  ccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTc                                                                                                                                                                                                                    <  1:573468/251‑1 (MQ=255)

                                  ccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGAACGTAACCCTGCGGATTGCGGCGTGACCACAGATGTGGGGCAGCTCCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCCCCTCCAGCGGCGTAGGCACCATGGGTTTTGGTGTCCCGGCCGCGG‑TTGGCGCGCGAGTCGCGCGGCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTc                                                                                                                                                                                                                    <  2:576619/251‑1 (MQ=255)

                                  ccGGGGACGCGATCTACGCGCCGTTGGTTTTAAAACAACTTGCAGAGCGTAAACCTGCGGATTGCGTCGGGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTc                                                                                                                                                                                                                    <  2:26417/251‑1 (MQ=255)

                                     gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGGCGCGCGACCCAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑atg                                                                                                                                                                                                                >  1:243637/1‑251 (MQ=255)

                                             cgcTACGGGCCGTTGTTGGTAAAACAACTGTCAGATCGTAATCATGCGGATTGCGTCGCGACCCCAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGAACATCGCCCACACT‑CGTCCGTCAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTTGCGCGCAAGTCGCGAGACCAAGC‑GTTAGCGTCGTCTGGATCTCC‑GGTGACGGCTCTTTC‑ATGATGAAtg                                                                                                                                                                                                         <  2:740172/248‑1 (MQ=255)

                                             aTCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑GTGGCGCGCCAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑CGTGACGGCTCTTTC‑ATGATGAAtgt                                                                                                                                                                                                        >  1:378951/1‑251 (MQ=255)

                                                 aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCaa                                                                                                                                                                                                    <  2:535633/251‑1 (MQ=255)

                                                   gcgcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTTGCGCGCAAGTCCCGCGCACAAAC‑GATACCGTCGTCTGTATCTCC‑CGGGGCGGCCTTTTC‑Atgatgacattgcaa                                                                                                                                                                                                    >  2:618744/1‑241 (MQ=255)

                                                     gcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACATCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGc                                                                                                                                                                                                <  1:705803/251‑1 (MQ=255)

                                                      ccGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCt                                                                                                                                                                                               <  2:569487/251‑1 (MQ=255)

                                                           gttgttAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGc                                                                                                                                                                                           <  1:542076/250‑1 (MQ=255)

                                                                 aaaaCAACTGTCAGATCGTAAACCTGCGGATTGCGTAGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGGGCGCAAGGCGCGCGACCAAAC‑GATACCGTCGTCTTTCTCCCC‑GGGGACGGCCCTTTC‑ATTATGAAAGGGCAAGAGCCGGGCCCCGTa                                                                                                                                                                                     >  2:281297/1‑250 (MQ=255)

                                                                    acaacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAAc                                                                                                                                                                                 <  2:665124/251‑1 (MQ=255)

                                                                     caacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑TGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACg                                                                                                                                                                                <  1:420134/251‑1 (MQ=255)

                                                                       acTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑GTGGCGCGCAAGTCGCGCGCCCAAAC‑GCTACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGCGCTGGGCACCGTAAAACGc                                                                                                                                                                               >  1:486765/1‑250 (MQ=255)

                                                                         tGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCt                                                                                                                                                                                               <  1:337847/232‑1 (MQ=255)

                                                                         tGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑GTTGCGCGCAAGTCGCGCGGCCAAAC‑AATACCGTCGTCTGTTTCTCC‑GGCGCCGGCTCTTTC‑ATGATGANTGTGCAAGAGCtagagcggacgagcgtcggg                                                                                                                                                                            >  2:337847/1‑232 (MQ=255)

                                                                           tcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGAACAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGaa                                                                                                                                                                                                                             >  1:657377/4‑200 (MQ=255)

                                                                           tcagATCGTAAACCTGCGGATTGCGGCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCTCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCGCC‑GGTGAc                                                                                                                                                                                                                             <  2:657377/198‑1 (MQ=255)

                                                                             agATCGTAAACCTGCGGATTGCGTCGTGCCCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCCGCGGGTTGG‑GCGCCAGTCGCTCGTCCAAAA‑GATACCGTCGTCTGTATCTCC‑GGGGGCGGGCCTTTCTAT‑ATGAATGTGCCAGAGGCGGGCACCGTCAAACGG‑AAgacg                                                                                                                                                                        >  2:42984/2‑247 (MQ=255)

                                                                              gATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGtt                                                                                                                                                                      <  1:541189/251‑1 (MQ=255)

                                                                               aacGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCCAGTCGCGCGACCAAAC‑GATACCGTCGGCTGGATCTCC‑GGTGCCGGCTCTTTC‑ATGGTGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑CAGCAGtt                                                                                                                                                                      >  2:442879/3‑250 (MQ=255)

                                                                                     aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGGCCAAAC‑GATACCGTCGACTGGATCCCC‑GGGGACGGCTCTTTC‑ATGAGGAATGTGCAAGAGCTGGGGACCGTAAAACGA‑CAGCAGTTACCGttt                                                                                                                                                               >  2:665323/1‑250 (MQ=255)

                                                                                     aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGGTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAACCGC‑AAGCAGTTACCGTTg                                                                                                                                                               <  2:637125/251‑1 (MQ=255)

                                                                                     aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGACCACACT‑CGCCCGGAAAATTTCATAACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGGCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGGGACGGCTCTTTC‑ATGATGAAAAGGCAAGAACTGGGCACCCTAAAAAGG‑ACGCCGTTACCGt                                                                                                                                                                 >  1:151043/1‑249 (MQ=255)

                                                                                         tGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑GTGGCGCGCAAGTCGCGCGACC‑AACAGATACCCTCGTCTGTATCTCC‑GGGGACGGCCCTTTCCTTGAT‑AATGTGCAAGAACTGGGACCCGGAAAAAGC‑AAGCAGTTAccctttaaa                                                                                                                                                            >  2:675206/1‑243 (MQ=255)

                                                                                            ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGCCGCCCGACCCAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGATGGGCAACGTAAAACGC‑AAGCAGTTACCGTTGTAAATCg                                                                                                                                                        >  1:188147/1‑251 (MQ=255)

                                                                                                tGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTTAAAATCG‑TCtt                                                                                                                                                   >  1:289963/1‑251 (MQ=255)

                                                                                                 gCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTa                                                                                                                                                  <  1:182203/251‑1 (MQ=255)

                                                                                                  cgtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTAc                                                                                                                                                 <  1:181156/251‑1 (MQ=255)

                                                                                                   gtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑GCTTACt                                                                                                                                                >  1:420906/1‑251 (MQ=255)

                                                                                                     cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCg                                                                                                                                              <  1:221396/251‑1 (MQ=255)

                                                                                                          ccACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAAc                                                                                                                                         <  1:226950/251‑1 (MQ=255)

                                                                                                           cacaGATGTGGGGCAGCACCAGATGTGGGCTNCGCAGCACATCGCCCACACG‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTTCCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAAcc                                                                                                                                        <  2:364230/251‑1 (MQ=255)

                                                                                                             caGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCGTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCCTACTCGATAACCaa                                                                                                                                      <  1:141587/251‑1 (MQ=255)

                                                                                                              aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGCCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑CCTTACTCGATAACCAAc                                                                                                                                     >  1:569973/1‑251 (MQ=255)

                                                                                                              aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAAc                                                                                                                                     >  1:457741/1‑251 (MQ=255)

                                                                                                              aGATGTGGGGCAGCACCAGAGGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCTGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAAc                                                                                                                                     <  2:453726/251‑1 (MQ=255)

                                                                                                                 tgtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACggg                                                                                                                                  >  1:254078/1‑250 (MQ=255)

                                                                                                                  gtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATACCCAACGGt                                                                                                                                  <  1:625851/250‑1 (MQ=255)

                                                                                                                      ggCAGCACCAGATGTGGGTTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCACCACCTCCAGCGGTTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑Aggg                                                                                                                            <  1:396193/251‑1 (MQ=255)

                                                                                                                      ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑Aggg                                                                                                                            <  2:552640/251‑1 (MQ=255)

                                                                                                                        cagcaCCAGATGTGGGCGGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGC‑AAGCAGGTACCGTTGAAAAGCG‑GCTTACTCGATAACCAACGGTT‑AGGGAt                                                                                                                          <  2:115165/251‑1 (MQ=255)

                                                                                                                         agcaCCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCTGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGGTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGGTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATg                                                                                                                         <  2:476263/251‑1 (MQ=255)

                                                                                                                         agcaCCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATg                                                                                                                         <  1:618744/251‑1 (MQ=255)

                                                                                                                         agcaCCAGATGTGCGCTGCGCAGCACATCGCCCACACTACGCC‑GGAAAATTTCATCACCACCAGCGGCTTGGGCCCCATAGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAGGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCACC‑GGTGACGGCTCTTTC‑ATGACGAATGTGCAAGAGCTGGGCCCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGaag                                                                                                                         <  1:618768/251‑3 (MQ=255)

                                                                                                                             ccAGATGTGGGCTGCGCAGCACACCGCCCACACT‑CGCCCGGACAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTGTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCTCCGTAAAACGC‑AAGTAGTGACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑ACGGATGGTTc                                                                                                                     <  2:586496/251‑1 (MQ=255)

                                                                                                                                     gggCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑CTGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAAACAACGGTT‑AGGGATGGTTCGACAATgg                                                                                                             >  1:78755/1‑251 (MQ=255)

                                                                                                                                      ggCTGCGCAGCCCATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgc                                                                                                            <  2:457741/251‑1 (MQ=255)

                                                                                                                                      ggCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgc                                                                                                            <  2:725086/251‑1 (MQ=255)

                                                                                                                                       gCTGCGCAGCACATCGCCCACACG‑CGCCCGGAAAAGTTCATCGCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgca                                                                                                           <  2:13017/251‑1 (MQ=255)

                                                                                                                                        cTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGGGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATggg                                                                                                            >  1:431737/1‑248 (MQ=255)

                                                                                                                                          gcgcAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgcag                                                                                                          <  1:281297/249‑1 (MQ=255)

                                                                                                                                          gcgcAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑ATGGCGAGCAAGTAGCGCGACCAAAA‑GATAACGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGGAAAAAGA‑AAGCAGTTACCGTTGGAAATCG‑TCTTACTAGGTAAACAACGGTT‑TGGGGTGGTTCGACAATGgcagc                                                                                                         >  2:699542/1‑250 (MQ=255)

                                                                                                                                          gagcagcaAATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTGTGGTTTACCGGCGGCGG‑TGGGCGCGCCAGGTGCGCGACCAAAC‑GAGACCGTCGTCTGGTTATCCAGGT‑ACGGCTCATTC‑ATGAAAAATGTGCCAAAGCGGGGCCCCGTAAAACGCCAACCCGT‑AACGTGTAAAATCG‑TATTACTCGATAACCAACGGGT‑AGGGATggggcgccaaggggcagc                                                                                                        >  2:509154/3‑235 (MQ=255)

                                                                                                                                              agcaCATCGCCCACACT‑CGCCCGGAAAATTTCATCCTCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TGGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTg                                                                                                    <  2:123058/251‑1 (MQ=255)

                                                                                                                                               gcaCATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGt                                                                                                   <  1:560036/251‑1 (MQ=255)

                                                                                                                                                 acaTCGCCCCCACT‑CGCCCGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGttt                                                                                                 <  2:563660/251‑1 (MQ=255)

                                                                                                                                                     cGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGtttttcc                                                                                             <  1:253827/251‑3 (MQ=255)

                                                                                                                                                      gCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCCGTTACCGTTGAAAATCG‑GCTTACTCGATAACCAACGGTT‑AAGGTTGGTTCGAACATGGCAGCAAATGtttttcc                                                                                             >  1:161037/1‑248 (MQ=255)

                                                                                                                                                        ccACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAgg                                                                                          <  1:442879/251‑1 (MQ=255)

                                                                                                                                                           cacT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAAc                                                                                       <  1:389026/251‑1 (MQ=255)

                                                                                                                                                                 gCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGAGCGCAAGTCGCGCGACCCAAC‑GATACCGGCGTCTGTATCTCC‑GGGGGCCGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGA‑AAGCAGTTACCGTTTAAAATCG‑GCTTACTCGATAACCAACGGTT‑AGGGATGGGTCGACAATGGGAGCAACTGtttttaccgggaagatat                                                                                  >  1:333164/1‑238 (MQ=255)

                                                                                                                                                                  cccGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATata                                                                                 <  2:128075/251‑3 (MQ=255)

                                                                                                                                                                   ccGGAAAATTTCACCACCCCCAGCGGCTTAGGCCCCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATatag                                                                                <  2:161037/251‑4 (MQ=255)

                                                                                                                                                                     ggAAAATTTCAGCACCTACAGCGGCCTAGGCACCATGGGTTTTGGGTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCGCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTGCCAGGAACGATATAGCg                                                                              <  2:642100/251‑1 (MQ=255)

                                                                                                                                                                        aaaTTTCATCACCTCCAGCGGCTTAGGCACCNTGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGaaa                                                                           <  2:379941/251‑1 (MQ=255)

                                                                                                                                                                          aTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAacc                                                                         >  1:38457/1‑251 (MQ=255)

                                                                                                                                                                                  cccTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGAAACGTAAAACGC‑AAGCAGTGACCGTTGAAAATCG‑TCTTACTCGATACCCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCttcc                                                                 <  2:198628/250‑3 (MQ=255)

                                                                                                                                                                                      ccAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGAt                                                             <  2:289963/251‑1 (MQ=255)

                                                                                                                                                                                        aGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGGCCAAAC‑GATACCGTCGGCTGTATCTCC‑GGGGACGGCTCTTTC‑ATGATGAATGTGCCAAAGCTGGGCACCGTAAAACGC‑AAGCCGTTACCGTTGAAAAACG‑TCTTACTCCATAACCAACGGTTCAGGG‑TGGGTCGCCAATGGGAGCCACTGTTTTTTCCGGGACGAAATAGCGAAACCCCCCTTAcccata                                                            >  2:523204/1‑245 (MQ=255)

                                                                                                                                                                                         gcgtcttAGGCACCATGGGTTTTTGTTTACCGGCGGCTG‑TTGGATCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGCCGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGC‑AAGTAGTTACTGTTGAAAATCG‑TCTTACTCGTTAACCAACGGTT‑AGTGATGGTGCGACAATGGCAGCAACTGTGTTTCCAGGACCGATATAGCGAAACCACCCTTACCGATa                                                            <  2:566421/244‑1 (MQ=255)

                                                                                                                                                                                         gcggctgaggcaCCACGGGGTTTGGGTTACCNGCGGCGG‑TTGTCGCGCACGTCGCGCGACCAAAC‑TATACCGTCGTCGGTATCTCC‑GGTGACGGCTCTTTC‑ATGAGGAATGTGCAAGAGCTGGGCACCGTCAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACGCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTGTCCAGGAACGATATAGCGAAACCATCCGTACCGATAAc                                                          <  2:388362/240‑1 (MQ=255)

                                                                                                                                                                                           ggTTTAGGCCCCATGGGTTTCTGTTTCCCGGNGGCGG‑TTGGCGTGCAAGACGCGCGTCCAATC‑GATACCGTCGTCTTTAACCCC‑GGGGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGTCATCGTAAAACAC‑AAGCAGTAGCCGTTGAAAATCG‑TCTTACTCTATATCCAACGGTT‑AGGGATGGTTCGACAATGTCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTCCCGATAAccc                                                        <  2:361831/251‑1 (MQ=255)

                                                                                                                                                                                            gcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGGTCGACAATGGCAGCAACTGGTTTTCCAGGGACGATATAGCGAAACCACCCTTAACGATAAcccc                                                       >  1:399912/3‑251 (MQ=255)

                                                                                                                                                                                               tAGGCACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCg                                                      <  1:678330/249‑1 (MQ=255)

                                                                                                                                                                                                aggcaCCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGAt                                                    <  2:38457/246‑1 (MQ=255)

                                                                                                                                                                                                  gcatcATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑AGGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTc                                                 <  2:243637/247‑1 (MQ=255)

                                                                                                                                                                                                     ccATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑TGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTc                                              <  1:703840/251‑1 (MQ=255)

                                                                                                                                                                                                      cATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGGTACCGTTGAAAATCG‑TCTTACTCGATAAACAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAACGAAAACACCCTTACCGATAACCCCGATTTCCTcc                                             >  1:354183/1‑250 (MQ=255)

                                                                                                                                                                                                               tGGTTGACCGGCGGCTG‑TTTGCGCGCCAGTCGCGCGATCAAAC‑GATACTGTCGTCTGTAGCTCC‑GGTGACGGCTCTTGC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGTTGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCGTACCGATAACCCCGATTTCCTCATGTTAGcc                                     <  2:108999/250‑1 (MQ=255)

                                                                                                                                                                                                                ggTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGGCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAAAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGCAAATCGGTCT‑ACTCGATAACCAACGGAT‑AGGGATGGTTCGACAATGGCAGCACATGTTTTTCCAGGACAGATATAGCGGAACCACCCTTACCCATAAACCCGATTTCCTCATGTTaacccg                                   >  2:202776/1‑246 (MQ=255)

                                                                                                                                                                                                                ggTTTACCGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGGATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAg                                   <  2:32431/251‑1 (MQ=255)

                                                                                                                                                                                                                      ccGGCGGCGG‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCtt                             <  2:25878/251‑1 (MQ=255)

                                                                                                                                                                                                                       cgtcggcgg‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCCCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTc                            <  2:420906/248‑1 (MQ=255)

                                                                                                                                                                                                                       cggcggcgg‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTc                            <  2:47396/251‑1 (MQ=255)

                                                                                                                                                                                                                       cggcggcgg‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTc                            <  1:764143/251‑1 (MQ=255)

                                                                                                                                                                                                                           ggcgg‑TTGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCtt                                                                                                                                                   <  1:93856/129‑1 (MQ=255)

                                                                                                                                                                                                                                  tGGCGCGCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATccct                   <  1:156791/250‑3 (MQ=255)

                                                                                                                                                                                                                                             ccgcgcgACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTGACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATc       <  2:238343/250‑1 (MQ=255)

                                                                                                                                                                                                                                                 gcgACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCAccc   <  2:172820/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  cgACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCg  <  1:675206/251‑1 (MQ=255)

CTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGG‑TTGGCGCACAAGTCGCGCGACCGAAC‑GATACCGTTGTCTGTATCTCC‑GGTGACGGCTCTTTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCG‑TCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCATGGCCAACACATCACCCG  >  NC_000913/3951566‑3952056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`