BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,754	T→C	intergenic (+1197/‑447)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,754	0	T	C	98.4%	412.5 / NA	121	intergenic (+1197/‑447)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (55/64); total (55/66)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 121 total aligned reads displayed.

GTTACCAGCATTACAGCAGCCGTTAA‑‑ATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑ACAAGTCGCGCGACCGAAC‑GATACCGTTGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCC  >  NC_000913/3951510‑3952002

gTTACCAGCATTACAGCAGCCGTTAAATATCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTa                                                                                                                                                                                                                                                              <  1:659542/250‑1 (MQ=255)

   aCCAGCATTACAGCAGCCGTTAAATATCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                                                                                                                                           <  1:276581/250‑2 (MQ=255)

      aGCATTACTGCATGCGTTAAATATCAATGACTGGCAGCTACACTGCTCGCAGCTGCGTGATGATCATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGGAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCTCCTCCAGCGGCTTAGGCACCa                                                                                                                                                                                                                                                       <  1:248361/251‑1 (MQ=255)

      aGCATTACAGCAGCCGTTAAATATCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACAACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCAccc                                                                                                                                                                                                                                                       >  1:26417/1‑250 (MQ=255)

                 agcCGTTAAATATCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGt                                                                                                                                                                                                                                            >  1:50987/1‑251 (MQ=255)

                   ccgttaaataTCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGttt                                                                                                                                                                                                                                          >  1:556238/10‑251 (MQ=255)

                    cgttaaataTCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGttt                                                                                                                                                                                                                                          >  1:238343/9‑250 (MQ=255)

                       taaataTCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcg                                                                                                                                                                                                                                      >  1:453726/6‑251 (MQ=255)

                         aataTCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggc                                                                                                                                                                                                                                    <  1:123968/248‑1 (MQ=255)

                            aTCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcgg                                                                                                                                                                                                                                  <  1:193810/250‑1 (MQ=255)

                              cAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcggcgg                                                                                                                                                                                                                               <  2:660120/251‑1 (MQ=255)

                              cAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcggcg                                                                                                                                                                                                                                <  1:607701/250‑1 (MQ=255)

                                aTGACTGGCAGCTACCCGGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGtt                                                                                                                                                                                                                             <  2:131575/251‑1 (MQ=255)

                                aTGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCAAATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCCTGGGTTTTGGTTTACcggcggcgggt                                                                                                                                                                                                                             >  2:602588/1‑249 (MQ=255)

                                 tcaCTGGCAGATACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAAAAATGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGAACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCCGCTTAGGCACCCTGGGGTTTGTTTTACCGGCGGCGGtat                                                                                                                                                                                                                            >  2:513729/3‑249 (MQ=255)

                                      ggcagcgtcacTGCGCGCAGCTGCGTGCTGAACATGCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCGGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcg                                                                                                                                                                                                                         <  2:724077/241‑1 (MQ=255)

                                         agctacacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAACCACCTGTCAGATCGTAAACCTGCGGATTGAGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcgcgcac                                                                                                                                                                                                                    >  1:255616/5‑247 (MQ=255)

                                          gctacacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTCCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACAGTCGGCGGtttggccgcc                                                                                                                                                                                                                     >  2:305457/4‑241 (MQ=255)

                                            tacacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTACAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGACAAgc                                                                                                                                                                                                                 >  2:109630/2‑250 (MQ=255)

                                             acacTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcggcgggtcggccccaaggcg                                                                                                                                                                                                                >  2:573468/1‑236 (MQ=255)

                                               acTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGCAAATTTCATCACCTCCAGCGGCTTCGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGgcgcgcacgtagcg                                                                                                                                                                                                              >  2:449477/1‑241 (MQ=255)

                                               acTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GGAAGTcgcg                                                                                                                                                                                                             >  1:586496/1‑251 (MQ=255)

                                                cTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTcgcgc                                                                                                                                                                                                            >  1:444105/1‑251 (MQ=255)

                                                  gcgcgcAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTCGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTcgcgc                                                                                                                                                                                                            <  1:424324/249‑1 (MQ=255)

                                                  gcgcgcAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCCAGTcgcgcg                                                                                                                                                                                                           >  1:47396/1‑250 (MQ=255)

                                                  gcgcgcAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGGcgcgcgc                                                                                                                                                                                                          >  1:379941/1‑250 (MQ=255)

                                                          cTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑g                                                                                                                                                                                                  >  1:404551/1‑250 (MQ=255)

                                                            gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GAt                                                                                                                                                                                                >  1:324719/1‑250 (MQ=255)

                                                                     aCATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCt                                                                                                                                                                                      >  1:364230/1‑251 (MQ=255)

                                                                      catgccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGATAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGACAGGAAAATTTCATCAACTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCCCGCCACCAAAC‑GAAACCGtttct                                                                                                                                                                                       >  1:361831/5‑246 (MQ=255)

                                                                           cTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTAtct                                                                                                                                                                                >  1:128075/1‑251 (MQ=255)

                                                                             ggCGTTACGACCATCCCGGTGACGCTAGCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTAtct                                                                                                                                                                                <  1:121960/249‑1 (MQ=255)

                                                                                gTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑ggg                                                                                                                                                                          >  1:25878/1‑250 (MQ=255)

                                                                                gTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGt                                                                                                                                                                          <  1:602588/251‑1 (MQ=255)

                                                                                 ttACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GGTACCGTCGTCTGTATCTCC‑gggg                                                                                                                                                                         >  1:32431/1‑249 (MQ=255)

                                                                                    cGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGGGACg                                                                                                                                                                      >  1:82507/1‑251 (MQ=255)

                                                                                      cccATCCCGGTGACGCGAGCTACGCGCCGTTGTTGTGAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGc                                                                                                                                                                    <  2:712219/250‑1 (MQ=255)

                                                                                       ccATCCCGGTGACGCTCTCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCGCC‑GGTGACGGct                                                                                                                                                                   <  2:67467/251‑1 (MQ=255)

                                                                                            ccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTc                                                                                                                                                             <  1:573468/251‑1 (MQ=255)

                                                                                            ccGGGGACGCGATCTACGCGCCGTTGGTTTTAAAACAACTTGCAGAGCGTAAACCTGCGGATTGCGTCGGGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTc                                                                                                                                                             <  2:26417/251‑1 (MQ=255)

                                                                                               gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGGCGCGCGACCCAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑atg                                                                                                                                                         >  1:243637/1‑251 (MQ=255)

                                                                                                       cgcTACGGGCCGTTGTTGGTAAAACAACTGTCAGATCGTAATCATGCGGATTGCGTCGCGACCCCAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGAACATCGCCCACACT‑CGTCCGTCAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGC‑GCAAGTCGCGAGACCAAGC‑GTTAGCGTCGTCTGGATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAAtg                                                                                                                                                  <  2:740172/248‑1 (MQ=255)

                                                                                                       aTCTTCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACTTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGAACAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAAtg                                                                                                                                                  <  2:464563/250‑1 (MQ=255)

                                                                                                       aTCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGC‑GCCAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑CGTGACGGCTCT‑TTC‑ATGATGAAtgt                                                                                                                                                 >  1:378951/1‑251 (MQ=255)

                                                                                                           aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCaa                                                                                                                                             <  2:535633/251‑1 (MQ=255)

                                                                                                             gcgcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGC‑GCAAGTCCCGCGCACAAAC‑GATACCGTCGTCTGTATCTCC‑CGGGGCGGCCTT‑TTC‑Atgatgacattgcaa                                                                                                                                             >  2:618744/1‑241 (MQ=255)

                                                                                                               gcCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACATCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGc                                                                                                                                         <  1:705803/251‑1 (MQ=255)

                                                                                                                ccGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCt                                                                                                                                        <  2:569487/251‑1 (MQ=255)

                                                                                                                     gttgttAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGc                                                                                                                                    <  1:542076/250‑1 (MQ=255)

                                                                                                                           aaaaCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCCAGTCGCCCGACCCAAC‑GATACCGTCGTCTGTATCTCC‑CGTGACGGC‑CTCTTC‑ATGATGGAAGGGCCATAGCTGGGCACCgga                                                                                                                              >  2:156791/1‑248 (MQ=255)

                                                                                                                           aaaaCAACTGTCAGATCGTAAACCTGCGGATTGCGTAGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGGGC‑GCAAGGCGCGCGACCAAAC‑GATACCGTCGTCTTTCTCCCC‑GGGGACGGCCCT‑TTC‑ATTATGAAAGGGCAAGAGCCGGGCCCCGTa                                                                                                                              >  2:281297/1‑250 (MQ=255)

                                                                                                                              acaacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAAc                                                                                                                          <  2:665124/251‑1 (MQ=255)

                                                                                                                               caacTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑TGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACg                                                                                                                         <  1:420134/251‑1 (MQ=255)

                                                                                                                                   tGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTTGCGC‑GCAAGTCGCGCGGCCAAAC‑AATACCGTCGTCTGTTTCTCC‑GGCGCCGGCTCT‑TTC‑ATGATGANTGTGCAAGAGCtagagcggacgagcgtcggg                                                                                                                     >  2:337847/1‑232 (MQ=255)

                                                                                                                                     tcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGAACAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGaa                                                                                                                                                                       >  1:657377/4‑200 (MQ=255)

                                                                                                                                        gATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGtt                                                                                                               <  1:541189/251‑1 (MQ=255)

                                                                                                                                         aacGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCCAGTCGCGCGACCAAAC‑GATACCGTCGGCTGGATCTCC‑GGTGCCGGCTCT‑TTC‑ATGGTGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑CAGCAGtt                                                                                                               >  2:442879/3‑250 (MQ=255)

                                                                                                                                               aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGGCCAAAC‑GATACCGTCGACTGGATCCCC‑GGGGACGGCTCT‑TTC‑ATGAGGAATGTGCAAGAGCTGGGGACCGTAAAACGA‑CAGCAGTTACCGttt                                                                                                        >  2:665323/1‑250 (MQ=255)

                                                                                                                                               aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAACCGC‑AAGCAGTTACCGTTg                                                                                                        <  2:637125/251‑1 (MQ=255)

                                                                                                                                               aaCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGACCACACT‑CGCCCGGAAAATTTCATAACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGGCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGGGACGGCTCT‑TTC‑ATGATGAAAAGGCAAGAACTGGGCACCCTAAAAAGG‑ACGCCGTTACCGt                                                                                                          >  1:151043/1‑249 (MQ=255)

                                                                                                                                                   tGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGC‑GCAAGTCGCGCGACC‑AACAGATACCCTCGTCTGTATCTCC‑GGGGACGGCCCT‑TTCCTTGAT‑AATGTGCAAGAACTGGGACCCGGAAAAAGC‑AAGCAGTTAccctttaaa                                                                                                     >  2:675206/1‑243 (MQ=255)

                                                                                                                                                      ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGCCGCCCGACCCAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGATGGGCAACGTAAAACGC‑AAGCAGTTACCGTTGTAAATCg                                                                                                 >  1:188147/1‑251 (MQ=255)

                                                                                                                                                           gCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTa                                                                                            <  1:182203/251‑1 (MQ=255)

                                                                                                                                                            cgtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTAc                                                                                           <  1:181156/251‑1 (MQ=255)

                                                                                                                                                             gtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGGGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGAAAAACGC‑AAGCAGTTACCGTTGAAAAACGTCTTACt                                                                                          >  1:172820/1‑251 (MQ=255)

                                                                                                                                                             gtcgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGGCTTACt                                                                                          >  1:420906/1‑251 (MQ=255)

                                                                                                                                                               cgtGACCACAGATGGGGGGCAGCACCAGATGTGTGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGTCTTAGGCACCATGGTTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTc                                                                                         <  2:556238/250‑1 (MQ=255)

                                                                                                                                                                    ccACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAc                                                                                   <  1:226950/251‑1 (MQ=255)

                                                                                                                                                                     cacaGATGTGGGGCAGCACCAGATGTGGGCTNCGCAGCACATCGCCCACACG‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTTCCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAAcc                                                                                  <  2:364230/251‑1 (MQ=255)

                                                                                                                                                                       caGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCGTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCCTACTCGATAACCaa                                                                                <  1:141587/251‑1 (MQ=255)

                                                                                                                                                                        aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGCCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGCCTTACTCGATAACCAAc                                                                               >  1:569973/1‑251 (MQ=255)

                                                                                                                                                                        aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                               >  1:457741/1‑251 (MQ=255)

                                                                                                                                                                        aGATGTGGGGCAGCACCAGAGGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCTGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                               <  2:453726/251‑1 (MQ=255)

                                                                                                                                                                            gtgGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATACCCAACGGt                                                                            <  1:625851/250‑1 (MQ=255)

                                                                                                                                                                                ggCAGCACCAGATGTGGGTTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCACCACCTCCAGCGGTTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGGCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑Aggg                                                                      <  1:396193/251‑1 (MQ=255)

                                                                                                                                                                                ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCTAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑Aggg                                                                      <  2:552640/251‑1 (MQ=255)

                                                                                                                                                                                  cagcaCCAGATGTGGGCGGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGC‑AAGCAGGTACCGTTGAAAAGCGGCTTACTCGATAACCAACGGTT‑AGGGAt                                                                    <  2:115165/251‑1 (MQ=255)

                                                                                                                                                                                   agcaCCAGATGTGCGCTGCGCAGCACATCGCCCACACTACGCC‑GGAAAATTTCATCACCACCAGCGGCTTGGGCCCCATAGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAGGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCACC‑GGTGACGGCTCT‑TTC‑ATGACGAATGTGCAAGAGCTGGGCCCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGaag                                                                   <  1:618768/251‑3 (MQ=255)

                                                                                                                                                                                       ccAGATGTGGGCTGCGCAGCACACCGCCCACACT‑CGCCCGGACAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTGTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCTCCGTAAAACGC‑AAGTAGTGACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑ACGGATGGTTc                                                               <  2:586496/251‑1 (MQ=255)

                                                                                                                                                                                                ggCTGCGCAGCCCATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgc                                                      <  2:457741/251‑1 (MQ=255)

                                                                                                                                                                                                ggCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgc                                                      <  2:725086/251‑1 (MQ=255)

                                                                                                                                                                                                    gcgcAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgcag                                                    <  1:281297/249‑1 (MQ=255)

                                                                                                                                                                                                    gagcagcaAATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTGTGGTTTACCGGCGGCGGTGGGCGC‑GCCAGGTGCGCGACCAAAC‑GAGACCGTCGTCTGGTTATCCAGGT‑ACGGCTCA‑TTC‑ATGAAAAATGTGCCAAAGCGGGGCCCCGTAAAACGCCAACCCGT‑AACGTGTAAAATCGTATTACTCGATAACCAACGGGT‑AGGGATggggcgccaaggggcagc                                                  >  2:509154/3‑235 (MQ=255)

                                                                                                                                                                                                        agcaCATCGCCCACACT‑CGCCCGGAAAATTTCATCCTCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTGGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTg                                              <  2:123058/251‑1 (MQ=255)

                                                                                                                                                                                                           acaTCGCCCCCACT‑CGCCCGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGttt                                           <  2:563660/251‑1 (MQ=255)

                                                                                                                                                                                                               cGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGtttttcc                                       <  1:253827/251‑3 (MQ=255)

                                                                                                                                                                                                                  ccACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAgg                                    <  1:442879/251‑1 (MQ=255)

                                                                                                                                                                                                                    acacT‑CGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCCACGGTT‑AGGGGTGGNTCGACAATGGCAGCAACTGTTTTTCCAGGa                                   >  2:389026/1‑250 (MQ=255)

                                                                                                                                                                                                                           gCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGAGC‑GCAAGTCGCGCGACCCAAC‑GATACCGGCGTCTGTATCTCC‑GGGGGCCGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGA‑AAGCAGTTACCGTTTAAAATCGGCTTACTCGATAACCAACGGTT‑AGGGATGGGTCGACAATGGGAGCAACTGtttttaccgggaagatat                            >  1:333164/1‑238 (MQ=255)

                                                                                                                                                                                                                            cccGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATata                           <  2:128075/251‑3 (MQ=255)

                                                                                                                                                                                                                             ccGGAAAATTTCACCACCCCCAGCGGCTTAGGCCCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATatag                          <  2:161037/251‑4 (MQ=255)

                                                                                                                                                                                                                              cGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGgca                                                     <  1:31840/223‑1 (MQ=255)

                                                                                                                                                                                                                               ggAAAATTTCAGCACCTACAGCGGCCTAGGCACCATGGGTTTTGGGTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCGCCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTGCCAGGAACGATATAGCg                        <  2:642100/251‑1 (MQ=255)

                                                                                                                                                                                                                                  aaaTTTCATCACCTCCAGCGGCTTAGGCACCNTGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGaaa                     <  2:379941/251‑1 (MQ=255)

                                                                                                                                                                                                                                    aTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAacc                   >  1:38457/1‑251 (MQ=255)

                                                                                                                                                                                                                                           caCCGCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGTCCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTGGAAAATCGTCTGACTCGATAACCAACTGTT‑AGGGATGGTTCGACAAGGGCAGCAACTGGTTTTCCAGGAACGAGATAGCGAAACCACCCtt             <  2:255616/250‑1 (MQ=255)

                                                                                                                                                                                                                                            cccTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGAAACGTAAAACGC‑AAGCAGTGACCGTTGAAAATCGTCTTACTCGATACCCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCttcc           <  2:198628/250‑3 (MQ=255)

                                                                                                                                                                                                                                                ccAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGACCAAAC‑GATACCGTCGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGAt       <  2:289963/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  aGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑GCAAGTCGCGCGGCCAAAC‑GATACCGTCGGCTGTATCTCC‑GGGGACGGCTCT‑TTC‑ATGATGAATGTGCCAAAGCTGGGCACCGTAAAACGC‑AAGCCGTTACCGTTGAAAAACGTCTTACTCCATAACCAACGGTTCAGGG‑TGGGTCGCCAATGGGAGCCACTGTTTTTTCCGGGACGAAATAGCGAAACCCCCCTTAcccata      >  2:523204/1‑245 (MQ=255)

                                                                                                                                                                                                                                                     ggTTTAGGCCCCATGGGTTTCTGTTTCCCGGNGGCGGTTGGCGT‑GCAAGACGCGCGTCCAATC‑GATACCGTCGTCTTTAACCCC‑GGGGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGTCATCGTAAAACAC‑AAGCAGTAGCCGTTGAAAATCGTCTTACTCTATATCCAACGGTT‑AGGGATGGTTCGACAATGTCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTCCCGATAAccc  <  2:361831/251‑1 (MQ=255)

GTTACCAGCATTACAGCAGCCGTTAA‑‑ATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACT‑CGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGC‑ACAAGTCGCGCGACCGAAC‑GATACCGTTGTCTGTATCTCC‑GGTGACGGCTCT‑TTC‑ATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGC‑AAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTT‑AGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCC  >  NC_000913/3951510‑3952002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`