Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,549	T→C	intergenic (+63/‑37)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,549	0	T	C	100.0%	216.3 / NA	62	intergenic (+63/‑37)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/33);  total (29/33)

CACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCT  >  NC_000913/536313‑536786                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              cACCTGCAAATTGCCGATAATCCGCATCGCNGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  <  2:350277/250‑1 (MQ=255) cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  >  1:350277/1‑250 (MQ=255) cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  <  2:149843/250‑1 (MQ=255)       cAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTTTTGTCCGAATTCAGGAAAAGCGAAAt                                                                                                                                                                                                                           <  2:720994/251‑1 (MQ=255)        aaaTTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGCAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACAACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAtt                                                                                                                                                                                                                          >  2:720562/1‑251 (MQ=255)         aaTTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATGATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAttt                                                                                                                                                                                                                         >  1:423053/1‑251 (MQ=255)          aTTGCCGATAATCCGCCTCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGAAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTAAGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCNGGTTGTCCGCATTCAGGAAAAGCGAAAttt                                                                                                                                                                                                                         >  2:387060/1‑250 (MQ=255)            tGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTaa                                                                                                                                                                                                                       <  1:277513/250‑1 (MQ=255)                  tAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGt                                                                                                                                                                                                                >  1:457006/1‑251 (MQ=255)                    aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGtt                                                                                                                                                                                                               >  1:597532/1‑250 (MQ=255)                    aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTGTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTa                                                                                                                                                                                                              <  2:415066/251‑1 (MQ=255)                      cccgcttgcggcgAACCGGGAACCGGGGAAATTAATTATGGTTATCCATTTTAGGTAATTGAAAAATCCGGAC‑ACAACGGTTGGGTTGGGTGGGAATATAAAACCCAAACCACCCCGGAAGCCGGGTTACGCTTGATGGATCCGTTACC‑TTAAAACGTTGCGCTATTCAGACAAAGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                            <  1:476612/244‑1 (MQ=255)                      ccGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                            <  1:290986/251‑1 (MQ=255)                         cATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑AACGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTAt                                                                                                                                                                                                         >  2:661215/1‑251 (MQ=255)                          aTCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                                                                         <  1:398398/218‑1 (MQ=255)                          aTCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                                                                         >  2:398398/1‑218 (MQ=255)                               ggcgAACCGGGAAACGGAGAAATTAATTAGGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACt                                                                                                                                                                                                   <  2:423053/251‑1 (MQ=255)                                   aaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                                                                >  1:559356/1‑250 (MQ=255)                                      cgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAttt                                                                                                                                                                                            <  1:100715/251‑1 (MQ=255)                                       gggaaccggAGAAATTAATTATGATTAGCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGA‑ACCGTTAAAACGTGACGCCATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGAAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTGCAAAGAGGTTAATTATGAGACTGGGAttt                                                                                                                                                                                            >  2:627479/1‑250 (MQ=255)                                         gaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTAtt                                                                                                                                                                                         <  1:16123/251‑1 (MQ=255)                                          aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  >  1:738200/1‑209 (MQ=255)                                          aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  <  2:738200/209‑1 (MQ=255)                                             cggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑GTCTGTCTACAACGGTTGGGTTGGGTGTGAAGATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTGTTTTAGGCCGCTAAGATGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTgtct                                                                                                                                                                                     <  2:436669/251‑4 (MQ=255)                                                agaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAg                                                                                                                                                                                  <  2:267894/251‑1 (MQ=255)                                                 gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGATGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAATCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGCAATTTAAGAGAGGTTAATTATGAAACTGGGATTTATAGGCTTAgg                                                                                                                                                                                 >  2:142966/1‑251 (MQ=255)                                                  aaaTTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGc                                                                                                                                                                                <  1:720562/251‑1 (MQ=255)                                                                 aTCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGAGCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtag                                                                                                                                                                  <  2:297870‑M1/250‑11 (MQ=255)                                                                       ttAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcat                                                                                                                                                              <  1:339109‑M1/248‑15 (MQ=255)                                                                          aGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagaca                                                                                                                                                        <  1:762042‑M1/251‑21 (MQ=255)                                                                           ggTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacat                                                                                                                                                       <  2:408121‑M1/251‑22 (MQ=255)                                                                                 cGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgc                                                                                                                                                 >  1:97759‑M1/1‑224 (MQ=255)                                                                                     aa‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggaattagacatttgtgcctg                                                                                                                                              >  2:564838‑M1/1‑220 (MQ=255)                                                                                         tCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGACTTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                                                                          >  2:714220‑M1/1‑217 (MQ=255)                                                                                           tGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcg                                                                                                                                         >  1:409470‑M1/1‑215 (MQ=255)                                                                                                      gttgggttggGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcg                                                                                                                             <  1:318501‑M1/251‑48 (MQ=255)                                                                                                           gttgggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCCGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTCgtagtcgtaggaattagacatttgtgcctgatgcgacgcttgacgcgtctt                                                                                                                         >  2:652011‑M1/1‑199 (MQ=255)                                                                                                                      atatAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctac                                                                                                              >  1:389035‑M1/1‑188 (MQ=255)                                                                                                                      atatAAACACCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGACTTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctac                                                                                                              >  2:62426‑M1/1‑188 (MQ=255)                                                                                                                         taAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgaagcgtcttatcaggcctacaacc                                                                                                          >  2:751781‑M1/1‑185 (MQ=255)                                                                                                                                 aatcctccacgtaagcCGTTTTACGCTGGTTGGATCCGG‑ACCGTTAAAACGTGAAGCTATTCTGACAATGATTTTTTTGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgatgcttgacgcgtcttatcaggcctacaaccggtgccgc                                                                                                  <  2:422639‑M1/245‑75 (MQ=255)                                                                                                                                         acGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGGAAAGCGAAACTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcttcttatcaggcctccaaccggtgccgcatccggc                                                                                           >  1:108776‑M1/1‑169 (MQ=255)                                                                                                                                                     ttACGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcaca                                                                               <  1:661215‑M1/250‑94 (MQ=255)                                                                                                                                                       aCGCTGGATGGATCCGT‑ACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaat                                                                             <  2:2013‑M1/250‑96 (MQ=255)                                                                                                                                                                     ccggaccgtTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatg                                                                <  2:747642‑M1/246‑109 (MQ=255)                                                                                                                                                                                    gttaagctgttcagtcaatgCTTTTTTAGGCCGCTAAGTTTGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcttacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                <  2:409470‑M1/242‑125 (MQ=255)                                                                                                                                                                                    gtgacgctatgtagaacattcttttttaggccgctaagttgGCAGGAGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTAAATGATGAAAATGGGATTTATTGGCTTAGGCATTAggagtcgtaggcattagacatttgtgcctgatgcgccgcttgacgcgtcttatctggccgacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttat                                                 <  2:7603‑M1/230‑124 (MQ=255)                                                                                                                                                                                      gacGCTATTCAGACAATACTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGATTTCAGGTAAAGCGAAATTTAAAAGCGGTTAATTATGCAACTGGGATTTATTGGATTAGGCATTAgtagtcgtaggcattagacatttgggcctgatgcgacgcttgacgcgtattatcaggcctacaacaggtgccgcatacggcaattggtgcacaatgactgatgagatgcttgacgcatcttttc                                                >  2:731858‑M1/2‑125 (MQ=255)                                                                                                                                                                                               cAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcctcttatcaggcctacaat                                     >  2:520255‑M1/1‑116 (MQ=255)                                                                                                                                                                                                 gACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgg                                   >  1:459928‑M1/1‑114 (MQ=255)                                                                                                                                                                                                 gACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggccaaccatgg                                   >  2:656850‑M1/1‑114 (MQ=255)                                                                                                                                                                                                  aCAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTACGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgtctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgaacaaagcctgatgcgatgcttgacgcatcttatcaggcctaccatggg                                  >  1:446797‑M1/1‑113 (MQ=255)                                                                                                                                                                                                            tttAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatc                         <  1:142966‑M1/250‑148 (MQ=255)                                                                                                                                                                                                             ttAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcg                        <  2:296239‑M1/250‑149 (MQ=255)                                                                                                                                                                                                                       aaGTTGGCAGGGGATCGTGTTGGCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccgg               <  2:230113‑M1/249‑158 (MQ=255)                                                                                                                                                                                                                       aaGTTGGCAGGGGAACGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggat             <  1:273923‑M1/251‑160 (MQ=255)                                                                                                                                                                                                                           tGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataagg         >  1:666061‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                             gcatgggatcgTGTGGTCCGAAGTCAGGAAAAGCGAAAGTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcg       <  2:520315‑M1/247‑166 (MQ=255)                                                                                                                                                                                                                              cAGGGGATGGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAttagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgt      <  1:516957‑M1/251‑167 (MQ=255)                                                                                                                                                                                                                              cAGGGGATCGTGTGGACCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgcagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggataggtaggccggataaggcgt      >  2:496270‑M1/1‑85 (MQ=255)                                                                                                                                                                                                                                ggggATCGTTTTGTCCGAAGTTAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCGTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatgggtgcacaatgcctgatgcgaggcttggcgcatcttatcaggcctacaatgtgtaccggatcggtaggccggataaggcgttt    <  2:260984‑M1/251‑169 (MQ=255)                                                                                                                                                                                                                                  ggATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGACACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggagcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttac  >  2:704005‑M1/1‑81 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              CACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAA‑TTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGT‑ACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCT  >  NC_000913/536313‑536786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑