Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,576	G→T	intergenic (‑154/‑152)	ibpA ← / → yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,576	0	G	T	100.0%	246.3 / NA	64	intergenic (‑154/‑152)	ibpA/yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/37);  total (27/37)

CTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGAGGGAAAG  >  NC_000913/3867336‑3867821                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cgggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATg                                                                                                                                                                                                                                              <  2:691344/248‑1 (MQ=255)    gctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCt                                                                                                                                                                                                                                          <  1:549829/251‑1 (MQ=255)     ctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCt                                                                                                                                                                                                                                          >  1:645615/1‑250 (MQ=255)         ggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGc                                                                                                                                                                                                                                     <  2:232856/251‑1 (MQ=255)                ttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGta                                                                                                                                                                                                                               <  1:94018‑M1/250‑3 (MQ=255)                ttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGt                                                                                                                                                                                                                                >  1:312514‑M1/1‑248 (MQ=255)                ttCTAAGTGGTTAAACAAACGGTAAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAAACAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGta                                                                                                                                                                                                                               <  2:4077‑M1/250‑3 (MQ=255)                     aGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttg                                                                                                                                                                                                                         >  2:460152‑M1/1‑243 (MQ=255)                      gTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGGCTTTTTTTTTCAGGTTATGGTCTATTGCAAAGtagatttgc                                                                                                                                                                                                                        <  2:686809‑M1/251‑10 (MQ=255)                               caaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgat                                                                                                                                                                                                                >  1:466010‑M1/1‑233 (MQ=255)                                           tCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgc                                                                                                                                                                                                   >  2:83053‑M1/1‑221 (MQ=255)                                                 aGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaat                                                                                                                                                                                             <  2:438098‑M1/251‑37 (MQ=255)                                                    aGAACGTTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCGCCTGAAAGCAGCTAGAATGTAAGACCTTCCGCAATGGACAGGTCAGGTAGCCAGACCACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCATTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaa                                                                                                                                                                                          <  2:706259‑M1/251‑40 (MQ=255)                                                        cGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataatt                                                                                                                                                                                                            >  1:130250‑M1/1‑208 (MQ=255)                                                        cGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCGTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataatt                                                                                                                                                                                                            <  2:130250‑M1/229‑22 (MQ=255)                                                          gTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggc                                                                                                                                                                                    >  1:630524‑M1/1‑206 (MQ=255)                                                            aaaGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcg                                                                                                                                                                                   <  1:235833‑M1/250‑47 (MQ=255)                                                              aGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg                                                                                                                                                                                  >  1:573794‑M1/1‑202 (MQ=255)                                                                cGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg                                                                                                                                                                                  >  1:729015‑M1/1‑200 (MQ=255)                                                                cGGGGATAAATCAAAGTTACGCATAATCAATAACGCATGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCGCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAGCTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatgtgcgggatgataattgcggattgcagaaataaaaagggcggagtt                                                                                                                                                                              <  2:121200‑M1/251‑52 (MQ=255)                                                                 ggggAGAAAGCAAAGATACGCATAATCAATAGCTCCGGAAATCAGCGAGAATGGAAGACCTGCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCATGCGCGTCCTCACCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTGTT‑CAAGCCTTATCAGCGGGCGTTTTTTTTCAGTTTATGGTCTAGAGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatg                                                                                                                                                                             <  2:456204‑M1/251‑53 (MQ=255)                                                                     aTAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatct                                                                                                                                                                         >  2:149319‑M1/1‑195 (MQ=255)                                                                          tCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CCAGCCTTATCAGCGGACTTTTTTTTTCATTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcc                                                                                                                                                                    >  2:180474‑M1/1‑190 (MQ=255)                                                                                 tactcataaTCAATAGCTCCTGAAACCAGCGAGAATGTAAGTCCTTCCACAATGGACAGGTCAGTTAGCCATAACACCCAACAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttc                                                                                                                                                              <  1:453005‑M1/246‑68 (MQ=255)                                                                                   cGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttctt                                                                                                                                                            >  1:485805‑M1/1‑181 (MQ=255)                                                                                     cATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGGCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttata                                                                                                                                                         <  1:485593‑M1/251‑73 (MQ=255)                                                                                        aaTCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagct                                                                                                                                                      <  1:406366‑M1/251‑76 (MQ=255)                                                                                        aaTCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagc                                                                                                                                                       <  1:318644‑M1/250‑75 (MQ=255)                                                                                             aTAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcgcttttcttatagcttcttg                                                                                                                                                 <  1:184974‑M1/251‑81 (MQ=255)                                                                                                      gAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcg                                                                                                                                        >  1:544577‑M1/1‑162 (MQ=255)                                                                                                             gCGAGAATGTAAGACCTTCCACAATGGACCGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagctgatctccgcccttntcttatagcttcttgccggaagcggcgtgac                                                                                                                                 >  2:334156‑M1/1‑155 (MQ=255)                                                                                                               gagaATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacg                                                                                                                               >  1:361112‑M1/1‑153 (MQ=255)                                                                                                                 gaATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGgagatttgcgggatggtaattgcggattgcagaaataaaaagggcggagatgagtcccgcccttttcttatagcttctttccggatgcggcgggaacgcc                                                                                                                             >  2:558405‑M1/1‑151 (MQ=255)                                                                                                                  aaTGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgcct                                                                                                                            <  1:591145‑M1/251‑102 (MQ=255)                                                                                                                   aTGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgcctt                                                                                                                           >  1:106493‑M1/1‑149 (MQ=255)                                                                                                                       aaGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatcc                                                                                                                       <  1:460152‑M1/251‑107 (MQ=255)                                                                                                                        aGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccg                                                                                                                      <  1:298988‑M1/251‑108 (MQ=255)                                                                                                                              tgcacaatGGACAGGTCAGGTAGCCAGAACCCCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcgtgccggatgcggcgtgaacgccttatccggccta                                                                                                                 <  2:122162‑M1/248‑113 (MQ=255)                                                                                                                               ccACAATGGACAGGTCAGGTAGCCAGAACATCCAACAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctac                                                                                                                <  2:204876‑M1/250‑114 (MQ=255)                                                                                                                                 acaATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaa                                                                                                              >  1:353692‑M1/1‑135 (MQ=255)                                                                                                                                       gACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatg                                                                                                       <  1:149319‑M1/251‑123 (MQ=255)                                                                                                                                          aggtcaggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaa                                                                                                     >  1:397133‑M1/1‑126 (MQ=255)                                                                                                                                                        gAACCCCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgc                                                                                      <  1:474092‑M1/251‑140 (MQ=255)                                                                                                                                                        gAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgc                                                                                      <  1:361316‑M1/251‑140 (MQ=255)                                                                                                                                                                  cAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttc                                                                             <  1:124426‑M1/250‑149 (MQ=255)                                                                                                                                                                        ggtctgcagcggctgcgatTTAAAAATGGGTCTGGAAATG‑ACTTTTGCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagatttgcgtaggcc                                                                      <  1:607546‑M1/242‑156 (MQ=255)                                                                                                                                                                        cgTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcc                                                                      <  1:144274‑M1/251‑156 (MQ=255)                                                                                                                                                                        cgTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcc                                                                      <  1:83053‑M1/251‑156 (MQ=255)                                                                                                                                                                         gTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcct                                                                     >  1:1824‑M1/1‑95 (MQ=255)                                                                                                                                                                              cATCGGCTACGATGTAAAAATGGGTCTGGAANTGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaaatcttgccaattcaatatattgcaggaccatgtaggcctgataa                                                                <  2:361112‑M1/251‑162 (MQ=255)                                                                                                                                                                                  ggCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCTGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttaaccgtcctacaaaaccatgcaaattccatacattgcaagcttatcgtacgacagataagcgt                                                            >  2:660073‑M1/1‑86 (MQ=255)                                                                                                                                                                                                 aTGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacg                                             <  1:334156‑M1/251‑181 (MQ=255)                                                                                                                                                                                                   gggTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatcccgcctacaaaatcctgaaaattcaatacattgcaagattttcgtaggactgataagcggagcgcatcacgcacgcc                                           >  2:235052‑M1/1‑69 (MQ=255)                                                                                                                                                                                                     aaaaaatatatctctttt‑caagccttaTCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcg                                         <  2:536349‑M1/238‑185 (MQ=255)                                                                                                                                                                                                             aTGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggattataattgcggattgcagaaataaaaagggcggagatgacctccgcccttttcttatagcttcttgccggatgcggcttgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggtta                                 <  2:397133‑M1/251‑193 (MQ=255)                                                                                                                                                                                                             aTGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggtt                                  >  1:740903‑M1/1‑59 (MQ=255)                                                                                                                                                                                                               gAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttag                                <  1:698861‑M1/250‑194 (MQ=255)                                                                                                                                                                                                                         aaGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaa                       >  1:502848‑M1/1‑48 (MQ=255)                                                                                                                                                                                                                                aTCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggagtgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttcgcgccattaaatatcgat               <  2:630524‑M1/251‑211 (MQ=255)                                                                                                                                                                                                                                  cAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggctgcggcgtgaacgccttatccggcctacaaaatcatgaaaattccatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatat             >  1:435851‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                                    gCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatggaaattcaaaacattgcaagattttcgtaggactgataagcgtagcgcatccggcacgctcgcatggttagcgccattaaatatacatattc           >  2:659403‑M1/1‑37 (MQ=255)                                                                                                                                                                                                                                       gACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgctcttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgcc        <  2:486959‑M1/251‑218 (MQ=255)                                                                                                                                                                                                                                            ttttttTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgccgcttt   >  1:349221‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                              ttttTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgccgctttc  <  1:361429‑M1/250‑224 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTT‑CAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGAGGGAAAG  >  NC_000913/3867336‑3867821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑