BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,091	A→G	N153N (AAT→AAC)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,091	0	A	G	98.4%	425.1 / ‑5.1	123	N153N (AAT→AAC)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (73/48); minor base T (0/2); total (73/50)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 124 total aligned reads displayed.

GCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACG  >  NC_000913/3815847‑3816333

gccgccTTCTGCGTCTCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGt                                                                                                                                                                                                                                               >  1:615787/1‑251 (MQ=255)

  cgccTTCTGCGTCGCTACAATGGATTCGCTTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCCGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGCCTTAAACGAATTCCCGATCGCAAATTCCTTCACCGTTTACAATACCGACAGAAACTCCGGCCACCCTCCCTTTCATCGGGTTGGtct                                                                                                                                                                                                                                             >  2:291257/1‑249 (MQ=255)

  cgccTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGttt                                                                                                                                                                                                                                             >  1:408197/1‑251 (MQ=255)

         tGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCATCCGTCCCCTGCCATTCCATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGTTTACAATACCGACAGAAACTGCGGCTCCAATCCCTTTCATAGGGTTGGTTTTCAGCtt                                                                                                                                                                                                                                      >  2:43602/1‑251 (MQ=255)

          gcgccGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCt                                                                                                                                                                                                                                       <  1:494258/245‑1 (MQ=255)

           cGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGc                                                                                                                                                                                                                                    <  1:400353/251‑1 (MQ=255)

                 tACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGtttt                                                                                                                                                                                                                              >  2:675160/1‑251 (MQ=255)

                   aaaTGGATTCGTGTCCCCCACGGGCCAGCGCAAACAAGGTTGGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCAACACTACGTTCATGGCGGTCTCGGCGGCTGAGTCTTCAACGTATTCCAGAGCGCAAATTTCTTCACCGTTTACAATACCGACAGAAACTGCGGCTTCCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTcc                                                                                                                                                                                                                            <  2:719131/250‑1 (MQ=255)

                      tGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTccacc                                                                                                                                                                                                                         >  1:195433/1‑251 (MQ=255)

                        gattcgattcCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAg                                                                                                                                                                                                                       <  1:210802/251‑1 (MQ=255)

                            cgattcCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTc                                                                                                                                                                                                                   <  1:675160/251‑1 (MQ=255)

                            cgattcCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTc                                                                                                                                                                                                                   >  1:686177/1‑251 (MQ=255)

                               tccccc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTAGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGATTTCAGCTTGACGTTTTCCACCAGCTTATGt                                                                                                                                                                                                                >  1:304856/3‑250 (MQ=255)

                                 cccc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGGCCCCTGCACTTCAATGAGGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgc                                                                                                                                                                                                             <  2:652634/251‑1 (MQ=255)

                                  ccc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAgcg                                                                                                                                                                                                            >  1:734745/1‑251 (MQ=255)

                                   cc‑TCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTATTCAACGTATTCCAGATCGCACATTGCTTCACCGTTTTCAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGtctcgc                                                                                                                                                                                                           >  2:578509/1‑246 (MQ=255)

                                         ggCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCCTCGGTAGCGCATCtg                                                                                                                                                                                                      >  2:255344/1‑250 (MQ=255)

                                          gCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtgc                                                                                                                                                                                                     >  1:15889/1‑249 (MQ=255)

                                             agcgCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAg                                                                                                                                                                                                  <  1:470245/248‑1 (MQ=255)

                                               cgCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAgcg                                                                                                                                                                                                <  2:745094/250‑1 (MQ=255)

                                                 ccAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGGTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAACGcc                                                                                                                                                                                              >  2:181959/1‑251 (MQ=255)

                                                 ccAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGACTTCTGCCGTCCCCTGCACTTCAATGATGCGCACGTCTTCGGTCATCACTACGTTCATGTCGGGCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACAATCCCTTTCATCGGGTTGGTTTTCAGCTTGCAGTTTTCCACCAGCTTCTGTAGCGCATCTGCCACCGcc                                                                                                                                                                                              >  1:432198/1‑251 (MQ=255)

                                                   aacaaGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCAc                                                                                                                                                                                            >  1:92371/1‑251 (MQ=255)

                                                      aaggtGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCa                                                                                                                                                                                         <  1:135374/247‑1 (MQ=255)

                                                       aggtGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAg                                                                                                                                                                                        <  1:151293/248‑1 (MQ=255)

                                                       aggtGAGTAGCTCTTCAGGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAg                                                                                                                                                                                        <  2:408197/248‑1 (MQ=255)

                                                         gtGAGTAGCTCTTCATGGGTGAACGGCTCGCCGTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACGACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCGACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGc                                                                                                                                                                                      <  2:136522/250‑1 (MQ=255)

                                                            aGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCAcc                                                                                                                                                                                   >  1:664798/1‑251 (MQ=255)

                                                             gtagctatttctGGGTGAACGGCGCGCCTTCTGCCGTCACCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGTTTACAATCCCGACAGAAACTGCGGCTACCATCCCTTTCGTCGGGTTGGTTTTCAGCTTGCCGTTTTCCTCCAGCTTCTGTAGCGCATCTGCCAGCGCCACTCAGGCAccc                                                                                                                                                                                  <  2:402768/240‑1 (MQ=255)

                                                              tAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCATTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGNAGCGCATCTGCCAGCGCCACGCAGGCAcccc                                                                                                                                                                                 >  2:373623/1‑250 (MQ=255)

                                                                gCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGt                                                                                                                                                                                >  1:115542/1‑250 (MQ=255)

                                                                   ctTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCCCCAGCTTCTGTAGCGNATCTGCCAGCGCCAAGCAGGCACCCGTAATc                                                                                                                                                                            >  2:356039/1‑251 (MQ=255)

                                                                      cATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCTGTCATCTCTACGTGCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTGTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGAc                                                                                                                                                                         <  1:712565/251‑1 (MQ=255)

                                                                                 ggCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGt                                                                                                                                                              >  1:523224/1‑251 (MQ=255)

                                                                                  gCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTg                                                                                                                                                             >  1:41493/1‑251 (MQ=255)

                                                                                    tCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCAGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGAATTCCCGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCCCCCGTAAACGACACGGTACGCGTgac                                                                                                                                                           >  2:507267/1‑249 (MQ=255)

                                                                                             gCCGTCCCCTGCACGTCAATGATGCGCCCGTCTGCGGTCATCTCTACGTTCATGTCGGTCTCTGCGGCTGATTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCTTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGTTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATc                                                                                                                                                     <  2:503134/248‑1 (MQ=255)

                                                                                                gTCCCCTGCTCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtgcc                                                                                                                                                                                                    <  1:272409/198‑4 (MQ=255)

                                                                                                gTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCCCCGTTTACAATACCGACCGAAACTGCGGCTACCATCCCTTTCATCGGGGTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtgcc                                                                                                                                                                                                    >  2:272409/1‑195 (MQ=255)

                                                                                                gTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTg                                                                                                                                               >  1:257186/1‑251 (MQ=255)

                                                                                                     cTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGcc                                                                                                                                          >  1:285388/1‑250 (MQ=255)

                                                                                                            tCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTAGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTANGCGTGCCACCATCAGCCTCAAGCACGACGc                                                                                                                                    >  2:361576/1‑250 (MQ=255)

                                                                                                            tCAATGATGCCCCCGTCTTCGGCCATCACTACGTTCATGTCGTTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTTGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCACCTGCCAGCGCCACGCAGGCACCCGTAATCGACACGGTACGCGTGCCACCATCAGCCTGAAGCCCGTc                                                                                                                                      <  1:507267/248‑1 (MQ=255)

                                                                                                                 gatgCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCCCCATCAGCCTGAAGCACGTCGCAGTccc                                                                                                                              >  2:242879/1‑250 (MQ=255)

                                                                                                                      gcCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCgg                                                                                                                          >  2:212640/1‑249 (MQ=255)

                                                                                                                       cccGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGGCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTGTTCAGCTTGCCGTTTTCCACCAGCTTCGGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCGCCATCAGCTTGAAGCACGTCGCAGTCCAGCGTaa                                                                                                                        <  1:618247/251‑1 (MQ=255)

                                                                                                                         cGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATg                                                                                                                      >  1:447502/1‑251 (MQ=255)

                                                                                                                           tCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCCCGTCGCAGTCCAGCGTAATggg                                                                                                                    >  2:713299/1‑250 (MQ=255)

                                                                                                                              tCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGa                                                                                                                  <  1:604604/250‑1 (MQ=255)

                                                                                                                                 gTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTc                                                                                                              >  1:386499/1‑251 (MQ=255)

                                                                                                                                 gTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTc                                                                                                              >  1:253107/1‑251 (MQ=255)

                                                                                                                                 atgcatgcgctctcaatgccgctctcTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACt                                                                                                               <  1:321193/228‑1 (MQ=255)

                                                                                                                                     tcaCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAccc                                                                                                          >  1:209503/1‑251 (MQ=255)

                                                                                                                                       aCTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAg                                                                                                        <  1:12502/251‑1 (MQ=255)

                                                                                                                                         tACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAgcg                                                                                                      >  1:720078/1‑251 (MQ=255)

                                                                                                                                          aCGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAgcgc                                                                                                     >  1:9376/1‑251 (MQ=255)

                                                                                                                                          aCGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGACCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCAGCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCGCGTCGCAGTCCCGCGTAATGGTGAACTCACCCAgcg                                                                                                      <  2:188276/250‑1 (MQ=255)

                                                                                                                                                   tCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCCGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCCCCCGTAATCGACGCGGTACGCGAGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGCGAACTCACCCAGCGCTTTCaaacc                                                                                            >  2:283664/1‑249 (MQ=255)

                                                                                                                                                        ctctGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTg                                                                                       >  1:145296/1‑251 (MQ=255)

                                                                                                                                                            gCGGCTGAGTCTTCAGCGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCCCCATCAGCATGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGc                                                                                      <  2:757509/248‑1 (MQ=255)

                                                                                                                                                            gCGGCTGAGTCTTCAACGTATTCCCGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGc                                                                                      <  2:523224/248‑1 (MQ=255)

                                                                                                                                                            gCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCcgc                                                                                   <  1:238483/251‑1 (MQ=255)

                                                                                                                                                            gCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCcgc                                                                                   >  1:526553/1‑251 (MQ=255)

                                                                                                                                                               gctgagTCTTCAACGTCTTCCAGATCGCAAATTGCTTCACCGTTTACAGTACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGATTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCCTCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGCAATCACCCAGCGCTTTCAAATCTACTGCcgcgcg                                                                                >  2:527460/4‑251 (MQ=255)

                                                                                                                                                                  gagTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGa                                                                               <  1:181959/249‑1 (MQ=255)

                                                                                                                                                                   agTCTTCAACGTATTCCAGATCGCAAATGTCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAag                                                                             <  2:734745/250‑1 (MQ=255)

                                                                                                                                                                        tCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACg                                                                       >  2:440543/1‑251 (MQ=255)

                                                                                                                                                                        tCAACGTAGTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCGGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCAc                                                                        <  2:148489/250‑1 (MQ=255)

                                                                                                                                                                          aaCGTATTCCAGATCGAAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCGTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCTCCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACggg                                                                     <  1:220580/251‑1 (MQ=255)

                                                                                                                                                                             gTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCa                                                                                                         >  2:588214/1‑212 (MQ=255)

                                                                                                                                                                             gTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCa                                                                                                         <  1:588214/212‑1 (MQ=255)

                                                                                                                                                                              tATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGAt                                                                 >  1:564996/1‑251 (MQ=255)

                                                                                                                                                                                ttCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGAGCTTTCCAATCTACTGCCGCGCGAAGAGCACGGGCGATc                                                                >  1:460688/1‑250 (MQ=255)

                                                                                                                                                                                   cAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGAc                                                            <  1:269124/251‑1 (MQ=255)

                                                                                                                                                                                      aTCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCt                                                         >  1:490879/1‑251 (MQ=255)

                                                                                                                                                                                           caattgcttcaccgtgtacaATACCGACAGAAACTGCGGCTCCCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGGAATCGACGCGGGACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACCTCTACTGCCGCGCGAAGAGCCCGGGCGATCAGCCGCTGCAtt                                                    >  2:84291/18‑251 (MQ=255)

                                                                                                                                                                                           aaattgCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGAtt                                                    >  2:614898/6‑251 (MQ=255)

                                                                                                                                                                                                  ttcaccGTTTACAATCCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGGTGGTTTTCAGCTTGCCGTTTCCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGGACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCTCGAAGAGCACGGGCGATCAGACGCTGGATTTCCAtg                                              <  2:193991/246‑2 (MQ=255)

                                                                                                                                                                                                  ttcaccGTTTACAATACCGACCGAAACTGCGGCTACCATCCCTTTCATCGTGTTGTTTTTCAGCTTGCCGTTTTCCACCAACTTCTGTAGCTCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGCACGCGGGCCACCATCAGCCAGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCACATCTACTGCCGCGCGAAGAGCACGGGCGATCAGAAGCTGGCGTTCCATtg                                             >  2:464794/5‑251 (MQ=255)

                                                                                                                                                                                                       cGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGt                                        >  2:759500/1‑251 (MQ=255)

                                                                                                                                                                                                        gtttacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGAGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCCGACGCTGGATTTCAATTGTGCGTc                                       >  2:255543/5‑251 (MQ=255)

                                                                                                                                                                                                         tttacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGGTTTCCATTGTGCGTcc                                      >  2:225210/4‑251 (MQ=255)

                                                                                                                                                                                                           tacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAACGCTTTCAAATCTACAGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCAc                                    >  1:506885/2‑251 (MQ=255)

                                                                                                                                                                                                           tacaATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGGAATAGACGCGGTACGAGTGCCACCATAAGCATGATGCACTACGCAGACCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTTCGGAGAGTAGAGCCCGGGCGATCAGACGGTGGGTTTCCATtgggggtcacc                                    >  2:761475/2‑242 (MQ=255)

                                                                                                                                                                                                              aataccGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCt                                 >  1:723766/5‑251 (MQ=255)

                                                                                                                                                                                                              aatacagagaGAAACTGCGGCTACCATCACTTTCCTCGGGTTGGTTTTCAGCTTGCCGTTTTCCCCCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCGGGCACCCGTAATCGACGCGGTACGCGTGCCAACCTCAGCCTGAAGCCCGCCGCAGCCCAGCGTAATGGTGAACTCACACAGCGCTTTCAAATCTACTGCCGAGCGAAGAGCACGGGCGATNAGCAGCGGGAGTTCCATGTTGCGTCCCCCCt                                 >  2:344938/10‑251 (MQ=255)

                                                                                                                                                                                                                    gACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCCCCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGAt                                                     >  2:117081/1‑225 (MQ=255)

                                                                                                                                                                                                                    gACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGAt                                                     <  1:117081/225‑1 (MQ=255)

                                                                                                                                                                                                                     actgAAACTCCGGCTCCCATCCCTTTCACCGGGTTGGGGTCCAGCTTTCCGTTTTCCCCTAGCTTCTGTGGCGCATCTGCCAGCGACACCCAGGCACCCTTAATCGACGCGGTACTCGCGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCAGCCAGCGCTTTCAAATCTACTTCCGCTCCAAGAGCACGGGCGATCAGACGCTGGATTTCCCTTGTGCGTCCACCCTGCTTa                            <  2:15889/246‑1 (MQ=255)

                                                                                                                                                                                                                     aCAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACACTGGATTTCCATTGTGCGTCCACCCTGCTTAcc                          >  1:477369/1‑251 (MQ=255)

                                                                                                                                                                                                                        gAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCttt                       <  1:36407/251‑1 (MQ=255)

                                                                                                                                                                                                                         aaaCTGCGGCCACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGGAGCGCAGCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTTCCGCGCGAAGAGCACTGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCGGCTTACCTTTc                      <  2:205057/251‑1 (MQ=255)

                                                                                                                                                                                                                            cTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGACCTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCCTTGTGCGTCCACCCTGCTTACCTTTcgcc                   >  2:168476/1‑251 (MQ=255)

                                                                                                                                                                                                                            cTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTcgcc                   >  2:551893/1‑251 (MQ=255)

                                                                                                                                                                                                                            cTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTcgcc                   >  1:565261/1‑251 (MQ=255)

                                                                                                                                                                                                                               cGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCCCCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGGCCACCCTGCTTACCTTTcgccgcc                >  2:413904/1‑250 (MQ=255)

                                                                                                                                                                                                                                        tCCCTTTCATCGGGTTGGTTTTCAGCTTGCCCTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCTCGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCCCGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCg       >  2:432545/1‑251 (MQ=255)

                                                                                                                                                                                                                                           cTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTGCCAGCGCCACGCAGGAACACGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGtt     >  1:655638/1‑250 (MQ=255)

                                                                                                                                                                                                                                             gtCATCGGGTTGGTTTGCAGCTGGCCGTTTTCCACCAGCTTCCGTAGCGCATCTGCCAGCGCCCCGCAGGCACCCGTAATCGACGCGGTCCGCGTGCAACCATCAGCCTGAAGCACGTCGCATTCCAGCGGAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCACTTCACGAGCGttcc   <  2:115542/249‑3 (MQ=255)

                                                                                                                                                                                                                                              gcATCGGTTTGGTTTTCAGCTTGCCGTTTTNCACCAGCTTCTGTAGCGCAGCTGCCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCAGTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACg  <  2:390482/249‑1 (MQ=255)

GCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACCAGCGCCACGCAGGCACCCGTAATCGACGCGGTACGCGTGCCACCATCAGCCTGAAGCACGTCGCAGTCCAGCGTAATGGTGAACTCACCCAGCGCTTTCAAATCTACTGCCGCGCGAAGAGCACGGGCGATCAGACGCTGGATTTCCATTGTGCGTCCACCCTGCTTACCTTTCGCCGCTTCACGAGCGTTACG  >  NC_000913/3815847‑3816333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`