| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,634 | A→G | D45D (GAT→GAC) | pyrE ← | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,634 | 0 | A | G | 98.9% | 313.8 / NA | 93 | D45D (GAT→GAC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (32/59); minor base T (0/1); total (33/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC > NC_000913/3815391‑3815880 | cccatcatCTAACAGCATTACGCGTCCTTGTAACGCGCTACTAACCAGAGTGCCGCCTTCACCGTGGTCTTTCGCCGCTTGGCGGTTAAAGAAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑ATTGCC‑AGGTCGCGcc < 2:331598/249‑1 (MQ=255)cacaTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCgcgc < 1:461766/250‑1 (MQ=255) ccatcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGccc < 2:536933/250‑1 (MQ=255) aGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGGCGGTAGGGGGCGCCATCGGGGTCCCTTTGTTAGCAAGGGCAAACCGCAGATCGCACTCCATGCCAGAATCCCCCAATGCTTCCGCGTAAAAACCGCCTAACCAG‑GCC‑AAGTCGCGCCCCGGATTAAAc > 1:706601/1‑249 (MQ=255) gCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGc < 2:84872/251‑1 (MQ=255) tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCg < 1:126447/250‑1 (MQ=255) cgTCCTTGTACCGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCAGGATGCGCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt < 2:515487/251‑1 (MQ=255) cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGTCGCCATCCGGAACCCTTTGTAAGCCGGGGCCAACAGCAGATCGAACTCCATGCCAGAATCCACCAAAGCTTCCGCGGAAAAAACGCCTAAA‑AATGCC‑CGGTAGCGCCCGGCATTAAACAGCCCGGCGtt > 2:66617/1‑251 (MQ=255) cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt < 2:3697/251‑1 (MQ=255) ggccTTGTAACGCGCTACCAACCAGATTGCCNCCTTCACCGGGGTCTTTTGCCTCTGTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCAACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGATCTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCTGTATTAAACAGCCCGGCGTTg < 2:342186/249‑1 (MQ=255) cctcgTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa < 1:683579/247‑1 (MQ=255) gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGc < 1:348279/251‑1 (MQ=255) aaccaGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgc < 2:307266/251‑1 (MQ=255) caGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGTCGCAATCGGGATCCCTTTTTAAGCCGGGCCAAACCGCAGAACGAACTCCATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCCAAC‑AATGCC‑CGGGCGCGCCCGGTATTAAACAGCCCCGCGGTGAAGAAATAAGGGCTTTTgcggc > 1:654874/1‑249 (MQ=255) aGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGccc < 2:763100/251‑1 (MQ=255) gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑tt < 1:409945/251‑1 (MQ=255) gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCCAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAACCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCAGGA‑tt < 1:449889/251‑1 (MQ=255) cgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGGCGCCATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGGATTAAAAAGCCCGGCGTTGGAGAAAAAAGGGCTTTTGCGCCCGGG‑tttt > 1:532983/1‑250 (MQ=255) tCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGc < 1:12760/249‑1 (MQ=255) aCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGAAGTGGTCGCCATACGGATCCCTTTGTAAGAAGGGGCAAACAGCAGAACGAACTCAAAGCCAGAAACCACCCATGCTTCAGCGTAAAAACGGGCTAAC‑AATGCCCAG‑GCGCGCCCGGTATTAAACAGACCGACGTGTAAGAAATAGGGGCTTTTTCGCCCGGAGTTTCacgta > 1:555646/1‑246 (MQ=255) ccGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTaaa < 1:254614/251‑1 (MQ=255) gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCATAATCCACCAATTCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt < 1:287802/251‑1 (MQ=255) gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGTCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt < 1:111336/251‑1 (MQ=255) ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc < 1:496505/250‑1 (MQ=255) ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc < 1:380240/250‑1 (MQ=255) ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCg < 1:326281/251‑1 (MQ=255) ggTCTTTTGCCGCTTTGCGGTTAAAGCAGGNCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc < 2:377878/250‑1 (MQ=255) cctgtgactttttGCGGTTAACGCTGTACGNGAGGTTGAGGTCATGATGCTCCGCCAGCGCTACGGAGGTAGTCGTCGCAATCGGGATCCCTTGGTAAGCTGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTATGCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTAAGCGTAAAATCGcc < 2:343210/240‑1 (MQ=255) ttttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa < 2:189182/250‑1 (MQ=255) tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa < 1:713375/249‑1 (MQ=255) ttGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTaa < 1:430913/248‑1 (MQ=255) ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgc < 1:188804/251‑1 (MQ=255) gTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCCCGGCGGTAGTGGTCGCTATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGACCTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgct < 1:383456/251‑1 (MQ=255) aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct < 1:665828/251‑1 (MQ=255) ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGAACGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa > 1:273190/1‑250 (MQ=255) gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt < 1:535397/251‑1 (MQ=255) gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt < 1:59480/251‑1 (MQ=255) aggtcaaggtcaTGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc < 1:368407/251‑1 (MQ=255) tcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTAAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAAATCCCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCaa > 1:719131/4‑250 (MQ=255) gtcatGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACt < 2:239696/247‑1 (MQ=255) gCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg < 2:715506/250‑1 (MQ=255) gCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg < 2:286183/250‑1 (MQ=255) cGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATg > 1:602887/1‑251 (MQ=255) cagcgCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACCGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCCCTgcatttggt > 2:400353/5‑243 (MQ=255) agcgCCACGGCGGTAGTGGGCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCAACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGCGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAATGGAGCTGATATGGttt > 2:507852/4‑251 (MQ=255) gcgCCACGGCGGGTGTGGGCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTc < 2:654874/249‑1 (MQ=255) ccACGGCGGTAGGGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCCAACAGCAGATCGGACTCAATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCTAAC‑AGGGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAAATTTAACACCTGCTTGCTAAGCGCCAATTCAATAAAATGGCGCTGATATgggtttatg > 2:145440/1‑244 (MQ=255) cacggCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGc < 1:619786/247‑1 (MQ=255) acggCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGcc < 1:333199/248‑1 (MQ=255) ggCGGTAGTGGTCGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCTCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAGATTCAATAAACTGGCGCTGATATTGTTGCATGCCtt < 1:622741/250‑1 (MQ=255) gtggtcGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGctcct > 1:761917/1‑251 (MQ=255) gtggtcGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACGCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGctcct < 2:50363/251‑1 (MQ=255) ggtcgCAATCGGGATCCCTTTGTAAGCAGGNCCAAACAGCAGATCGAACGCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGctcctc < 2:353894/246‑1 (MQ=255) ggtcgCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGACCTCAATGCCAGAATCCACCAATGCTTCAGCGTAAACACGGCCTAAC‑AGTGCC‑AGTTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TGTCAGCGTAAACTCGCCAAACTGTAACACCTGCTTGCTAAGCGCAAATGCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGctcctc < 2:265356/246‑1 (MQ=255) aaatggggATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGAGTAAAAAAGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACTCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTTCTCATCTTa < 2:706601/246‑1 (MQ=255) aaTCGGGATCCCTTTGTAAGCAGGGACAAACAGCAGATCGACCTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAc > 1:599708/1‑251 (MQ=255) aTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACt > 1:515215/1‑251 (MQ=255) gggATCCCTTTGTAAGAAGGGCCAAACCGCAGATCGAACTCAATGCCAGAATCCACCAATGATTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACtttt > 1:649173/1‑251 (MQ=255) gATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt < 1:400911/251‑1 (MQ=255) gATCCCTTTGTAAGCAGGGCCAAACAGAAGATCGACCTCAATGCCAGATACCTCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTTCTAAACCGCCCGGCGTTTAACAAATAGTGGCTTTTGCGCCTGTA‑TTTCAGCGTAAACTCGCCAAACTTGAACACCTGCTTTCTTAGCGCAAATTCAATATACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCt < 2:273190/251‑1 (MQ=255) cccTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTc < 2:412021/228‑1 (MQ=255) cccTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTc > 1:412021/1‑228 (MQ=255) gTAAGCAGGGCCAANCAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCtt < 2:365413/234‑1 (MQ=255) gTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCtt > 1:365413/1‑234 (MQ=255) cAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAac < 2:428478/161‑1 (MQ=255) cAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAac > 1:428478/1‑161 (MQ=255) aaggCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGACGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAAACCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCATTCGCTCCTCATCTTACTTTTCTACAGACaaaaacacg > 2:423312/3‑247 (MQ=255) aGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg < 1:578185/251‑1 (MQ=255) gggCCAAACAGCAGATCGAACTCAATGCCATAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTTCTTTTCTACAGACAAAAAAAAgg < 1:651350/251‑1 (MQ=255) ggCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGc < 2:53674/251‑1 (MQ=255) ccAAACAGCAGATCGAACTCACTGCCCGAATCCACCAATGCTTCAGCGTAAAACCGGCCTAAC‑AGTGCC‑AGGCCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTCCGCCCGTA‑TCTCAGCGTACACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTACTCTTACTTTTCTACCGCCaaaacaacgccg > 2:712565/1‑242 (MQ=255) aaCAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtc > 1:754788/1‑251 (MQ=255) aCAGCAGAACGAACTCAATGCCATAATCCACCAATGCTGCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACGTTTCTACAGACAAAAAAAAGGCGACtca < 2:532983/251‑1 (MQ=255) gatcgatcTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTTCC‑AGGTCGCGCCCGGTAGTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGTA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTGCAGACAAAAAAAATGCGACTCATCAGTc < 2:761917/251‑1 (MQ=255) gATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGATAAAAAAAAGGCGACTCATCAGTc > 1:437118/1‑251 (MQ=255) cAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCACCCGGTATTAAACAGACCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACTTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATACCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCttcaa > 2:494258/1‑247 (MQ=255) aaTGCCAGAATCCACCAAGGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTAGCGCCCGGTATTAAACAGCCCGGCGTGGAAGAAATAGGGGTTGTTGCGCCCGGA‑TTCCAGCGTAAACTCGCCAAACGTTAACACCGGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATTAGTCGCCTTaaaaa < 2:74029/251‑1 (MQ=255) aTGCCCGAATCCACCATGGGTGCAGCGTAAACACGGCATAAC‑AGTGTC‑AGGTCGCACCCGGTATTAAACAGCCCGGCGTTGAACAAAGAGGGGCTTTTGCGACCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCGGCTTGCTAAGCGCAAATTCAATAAACTGTCGCTGATATGGTATCATGCCTTCGCTCCTCCTCTTACTTATCTACAGACAAAAAAAAGGCGACTCATCAGTCTCCTTaaaaag < 2:275483/251‑2 (MQ=255) ccagAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc > 1:523267/4‑249 (MQ=255) agAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTAGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGTCGACTCATCAGTCGCCTTAAAACTCAGtt > 2:577556/2‑251 (MQ=255) gAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTAAATAAACTGGAGCTGATATGGTTTCATGCCTTCGCTCCTCCTCTTACTTTTCTACCAACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGttt > 2:532581/1‑251 (MQ=255) caccaATGCTTCAGCGTAAAAACGGCCTAAC‑AGAGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCCCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAg < 1:369995/251‑1 (MQ=255) tgCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc > 1:550095/2‑251 (MQ=255) ttCAGCGTAAAAACGGCCTAAA‑AGTGCC‑AGGTCGAGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCACCTCATATTACTTTTCTACAGACAAAAAAAAGACGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt > 1:86283/1‑251 (MQ=255) aaacggccgaacggctcc‑AGGTCGCGCCCGGTCTTAAACAGCCCGGCGTTGAAGAAATAGGTGCTTGTGCGCCCGGA‑TTGCAGCGTAAACTCGCCAAACTTTAACTCCAGCTTGCTAAGCGCAAATACAATAAACTGGCGCTGATATGGTTTCATGCCTGCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGGCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt < 2:4788/235‑1 (MQ=255) ggCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa > 2:606690/1‑250 (MQ=255) gCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt > 2:758762/1‑251 (MQ=255) aaC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat < 1:490257/251‑1 (MQ=255) aC‑AGGGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCATTGAAGAAATAGGGGTGTTTGCCCGCGGA‑GTTCAGCGTAAACGCGCCAAACTTTATCACCTGCCTGATAAGCTCAAATTCAATAAACTGTAACTGATATGGTTACATGACTTCGATCCCCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGACAGCGCCGCCGTCTGCGTCGCTACAAGGgatt < 2:17120/251‑1 (MQ=255) c‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCACCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTACCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc > 1:668967/1‑251 (MQ=255) gTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg > 1:481548/1‑250 (MQ=255) gTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg > 1:289564/1‑250 (MQ=255) cc‑AGGTCGCGCCCGGTAGTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TGTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTTCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTGCTGCGTCGCTACAATGgattcgattc < 2:712213/251‑1 (MQ=255) | CACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC > NC_000913/3815391‑3815880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |