Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,529	T→A	T80T (ACA→ACT)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,529	0	T	A	97.6%	290.3 / NA	84	T80T (ACA→ACT)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (13/69);  total (14/70)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTT  >  NC_000913/3815285‑3815773                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         cgcgCCCCCGTTCCTGACGATCAAGCGAAATCAATCCGCCAGCAAGCTGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtc                                                                                                                                                                                                                                                    <  2:515153/251‑2 (MQ=255) cgcgCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtc                                                                                                                                                                                                                                                    <  1:52170/251‑2 (MQ=255)  gcgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtcg                                                                                                                                                                                                                                                   <  2:696029/251‑3 (MQ=255)   cgctcccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtcgc                                                                                                                                                                                                                                                  <  1:729566/245‑4 (MQ=255)     cccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGGTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCaa                                                                                                                                                                                                                                                <  1:351316/247‑1 (MQ=255)      ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAAt                                                                                                                                                                                                                                               <  2:240874/248‑1 (MQ=255)            cccTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACAGCATCTACCAGCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTTCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑At                                                                                                                                                                                                                                        <  2:175822/250‑1 (MQ=255)             ccTGACGATCAAGCGAAATCAATCCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCTCGG‑CGGTAGTGGTCGCAATCGGG‑ATc                                                                                                                                                                                                                                       <  1:671277/251‑1 (MQ=255)               ggACGATCAAGCGAAATCAATACGCCAGCAANCGTCGCCCCATTCGCCTGAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATTTTCCAGCATTACGCGACCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATccc                                                                                                                                                                                                                                     <  2:368143/250‑1 (MQ=255)                  cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCttg                                                                                                                                                                                                                                  <  2:487258/251‑2 (MQ=255)                    atcaaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGt                                                                                                                                                                                                                                <  1:345796/247‑1 (MQ=255)                          cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAg                                                                                                                                                                                                                          <  1:221125/251‑1 (MQ=255)                          cGAAATCAAAACGCCAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCAGCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTACCGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAg                                                                                                                                                                                                                          <  2:475513/251‑1 (MQ=255)                            aaaTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAggg                                                                                                                                                                                                                        <  1:267607/251‑1 (MQ=255)                             aaacaatacGCCAGCAAGCGTCGCGCCATTANCCTGAATAATCTCCATCGATGCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGc                                                                                                                                                                                                                       <  2:350629/244‑1 (MQ=255)                              aTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGcc                                                                                                                                                                                                                      <  1:145037/251‑1 (MQ=255)                                       ccAGCAAGCGTCGCGCCAGTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATGACGCGTCCTTGTAACGCGCCACCAACCAGATTGCCGCCTTCACCGTTGTCTTTTGCCTCTTTGCGGGTAAAGCAGCACGGCAGTTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAAcagcag                                                                                                                                                                                                             <  2:244346/251‑1 (MQ=255)                                           cAAGCGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAAGCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCg                                                                                                                                                                                                         <  2:577760/251‑1 (MQ=255)                                             aGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATTCTCCGCCCGCGCCACGG‑CGGGAGTGGTCGCAAACGGG‑GTCCCCTTGTAAGCAGGGCCaaaaagacgaacga                                                                                                                                                                                                        >  1:84872/1‑239 (MQ=255)                                                   gcgcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCTTTGGGGTTAAAGCAGTACGTCAGGTCAAGGGCATGATGCTCCGCCAGCGCGTCGG‑CGGTAGTTGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAAt                                                                                                                                                                                                 <  1:435270/251‑1 (MQ=255)                                                     gcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCAGTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGc                                                                                                                                                                                               <  1:387123/251‑1 (MQ=255)                                                      ccATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCTTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGcc                                                                                                                                                                                              <  1:708632/251‑1 (MQ=255)                                                           agCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCCTGATGCTCCGCCCGCGCC‑CAGGCGGGTGTGGTCGCAAACGGGGATCC‑TTTGTAAGCCGGGGCAAAcacccggtcggaatcaatgcccaaa                                                                                                                                                                                          >  1:50363/2‑227 (MQ=255)                                                                  ataatCTCCACCGATGCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCGGCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGTCCAATCAGCAGATCGAACTCAATGCCAGAATCCTCCaa                                                                                                                                                                                  <  2:212675/251‑1 (MQ=255)                                                                         ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCt                                                                                                                                                                              <  1:517035/248‑1 (MQ=255)                                                                             cgattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGt                                                                                                                                                                       <  1:516382/247‑1 (MQ=255)                                                                                         cGCCTTTCCGGCGGGGATCACAGCATCTACCAGCATTACGCGTCCTTTTAACGCGCTACCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCGCGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCt                                                                                                                                                            <  2:256392/250‑1 (MQ=255)                                                                                         cGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTa                                                                                                                                                           <  1:172190/251‑1 (MQ=255)                                                                                          gCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTaa                                                                                                                                                          <  1:569429/251‑1 (MQ=255)                                                                                                ccGGCTGTGATCCCATCATCTACCAGCATTACGCGGCCTTGTAACGCGCGACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCTCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGc                                                                                                                                                   <  2:654657/251‑1 (MQ=255)                                                                                                ccGGCGGTGATCACATCATCTACCAGCATTANGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGc                                                                                                                                                   <  2:355911/251‑1 (MQ=255)                                                                                                      gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGGAGTGGTCGCCATCCGG‑ATCCCTTTGTAAGCCGGGCCCAACAGCCGATCGAACCCCATGCCAGAAACCACCAATGCTTCAGCGTAAAAACGGCCCAAC‑AGTGcccaggc                                                                                                                                             >  1:377878/1‑246 (MQ=255)                                                                                                        gATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGcc                                                                                                                                                                                              <  1:151135/201‑1 (MQ=255)                                                                                                           cccatcatCTAACAGCATTACGCGTCCTTGTAACGCGCTACTAACCAGAGTGCCGCCTTCACCGTGGTCTTTCGCCGCTTGGCGGTTAAAGAAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑ATTGCC‑AGGTCGCGcc                                                                                                                                       <  2:331598/249‑1 (MQ=255)                                                                                                           cacaTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCgcgc                                                                                                                                        <  1:461766/250‑1 (MQ=255)                                                                                                            ccatcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGccc                                                                                                                                      <  2:536933/250‑1 (MQ=255)                                                                                                                        aGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGGCGCCATCGGG‑GTCCCTTTGTTAGCAAGGGCAAACCGCAGATCGCACTCCATGCCAGAATCCCCCAATGCTTCCGCGTAAAAACCGCCTAACCAG‑GCC‑AAGTCGCGCCCCGGATTAAAc                                                                                                                            >  1:706601/1‑249 (MQ=255)                                                                                                                         gCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGc                                                                                                                         <  2:84872/251‑1 (MQ=255)                                                                                                                             tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCg                                                                                                                      <  1:126447/250‑1 (MQ=255)                                                                                                                                 cgTCCTTGTACCGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCAGGATGCGCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt                                                                                                                 <  2:515487/251‑1 (MQ=255)                                                                                                                                 cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCACGG‑CGGGAGTGGTCGCCATCCGG‑AACCCTTTGTAAGCCGGGGCCAACAGCAGATCGAACTCCATGCCAGAATCCACCAAAGCTTCCGCGGAAAAAACGCCTAAA‑AATGCC‑CGGTAGCGCCCGGCATTAAACAGCCCGGCGtt                                                                                                                 >  2:66617/1‑251 (MQ=255)                                                                                                                                 cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt                                                                                                                 <  2:3697/251‑1 (MQ=255)                                                                                                                                  ggccTTGTAACGCGCTACCAACCAGATTGCCNCCTTCACCGGGGTCTTTTGCCTCTGTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCAACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGATCTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCTGTATTAAACAGCCCGGCGTTg                                                                                                                <  2:342186/249‑1 (MQ=255)                                                                                                                                    cctcgTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                                                                                              <  1:683579/247‑1 (MQ=255)                                                                                                                                               gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGc                                                                                                   <  1:348279/251‑1 (MQ=255)                                                                                                                                                     aaccaGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgc                                                                                             <  2:307266/251‑1 (MQ=255)                                                                                                                                                        caGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGTCGCAATCGGG‑ATCCCTTTTTAAGCCGGGCCAAACCGCAGAACGAACTCCATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCCAAC‑AATGCC‑CGGGCGCGCCCGGTATTAAACAGCCCCGCGGTGAAGAAATAAGGGCTTTTgcggc                                                                                          >  1:654874/1‑249 (MQ=255)                                                                                                                                                         aGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGccc                                                                                         <  2:763100/251‑1 (MQ=255)                                                                                                                                                              gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑tt                                                                                   <  1:409945/251‑1 (MQ=255)                                                                                                                                                              gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCCAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAACCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCAGGA‑tt                                                                                   <  1:449889/251‑1 (MQ=255)                                                                                                                                                                cgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGGCGCCATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGGATTAAAAAGCCCGGCGTTGGAGAAAAAAGGGCTTTTGCGCCCGGG‑tttt                                                                                 >  1:532983/1‑250 (MQ=255)                                                                                                                                                                     tCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGc                                                                              <  1:12760/249‑1 (MQ=255)                                                                                                                                                                       aCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGAAGTGGTCGCCATACGG‑ATCCCTTTGTAAGAAGGGGCAAACAGCAGAACGAACTCAAAGCCAGAAACCACCCATGCTTCAGCGTAAAAACGGGCTAAC‑AATGCCCAG‑GCGCGCCCGGTATTAAACAGACCGACGTGTAAGAAATAGGGGCTTTTTCGCCCGGAGTTTCacgta                                                                            >  1:555646/1‑246 (MQ=255)                                                                                                                                                                        ccGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTaaa                                                                         <  1:254614/251‑1 (MQ=255)                                                                                                                                                                          gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCATAATCCACCAATTCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt                                                                       <  1:287802/251‑1 (MQ=255)                                                                                                                                                                          gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGTCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt                                                                       <  1:111336/251‑1 (MQ=255)                                                                                                                                                                            ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                      <  1:380240/250‑1 (MQ=255)                                                                                                                                                                            ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                      <  1:496505/250‑1 (MQ=255)                                                                                                                                                                            ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCg                                                                     <  1:326281/251‑1 (MQ=255)                                                                                                                                                                            ggTCTTTTGCCGCTTTGCGGTTAAAGCAGGNCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                      <  2:377878/250‑1 (MQ=255)                                                                                                                                                                               cctgtgactttttGCGGTTAACGCTGTACGNGAGGTTGAGGTCATGATGCTCCGCCAGCGCTACGG‑AGGTAGTCGTCGCAATCGGG‑ATCCCTTGGTAAGCTGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTATGCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTAAGCGTAAAATCGcc                                                                   <  2:343210/240‑1 (MQ=255)                                                                                                                                                                                ttttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa                                                                  <  2:189182/250‑1 (MQ=255)                                                                                                                                                                                 tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa                                                                  <  1:713375/249‑1 (MQ=255)                                                                                                                                                                                     cctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAc                                                                                                                                                         <  2:70603/159‑1 (MQ=255)                                                                                                                                                                                          ttGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTaa                                                          <  1:430913/248‑1 (MQ=255)                                                                                                                                                                                              ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                                   <  1:188804/251‑1 (MQ=255)                                                                                                                                                                                               gTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCCCGG‑CGGTAGTGGTCGCTATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGACCTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgct                                                  <  1:383456/251‑1 (MQ=255)                                                                                                                                                                                                   aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct                                              <  1:665828/251‑1 (MQ=255)                                                                                                                                                                                                            ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGAACGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                                      >  1:273190/1‑250 (MQ=255)                                                                                                                                                                                                             gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                    <  1:535397/251‑1 (MQ=255)                                                                                                                                                                                                             gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                    <  1:59480/251‑1 (MQ=255)                                                                                                                                                                                                               aggtcaaggtcaTGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                                  <  1:368407/251‑1 (MQ=255)                                                                                                                                                                                                                  tcaaggtcATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTAAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAAATCCCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCaa                                >  1:719131/4‑250 (MQ=255)                                                                                                                                                                                                                       gtcatGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACt                          <  2:239696/247‑1 (MQ=255)                                                                                                                                                                                                                               gCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg                   <  2:286183/250‑1 (MQ=255)                                                                                                                                                                                                                               gCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg                   <  2:715506/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATg              >  1:602887/1‑251 (MQ=255)                                                                                                                                                                                                                                      cagcgCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACCGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCCCTgcatttggt           >  2:400353/5‑243 (MQ=255)                                                                                                                                                                                                                                       agcgCCACGG‑CGGTAGTGGGCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCAACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGCGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAATGGAGCTGATATGGttt          >  2:507852/4‑251 (MQ=255)                                                                                                                                                                                                                                        gcgCCACGG‑CGGGTGTGGGCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTc         <  2:654874/249‑1 (MQ=255)                                                                                                                                                                                                                                           ccACGG‑CGGTAGGGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCCAACAGCAGATCGGACTCAATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCTAAC‑AGGGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAAATTTAACACCTGCTTGCTAAGCGCCAATTCAATAAAATGGCGCTGATATgggtttatg      >  2:145440/1‑244 (MQ=255)                                                                                                                                                                                                                                            cacgg‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGc     <  1:619786/247‑1 (MQ=255)                                                                                                                                                                                                                                             acgg‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGcc    <  1:333199/248‑1 (MQ=255)                                                                                                                                                                                                                                               gg‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCTCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAGATTCAATAAACTGGCGCTGATATTGTTGCATGCCtt  <  1:622741/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         CGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTT  >  NC_000913/3815285‑3815773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑