Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,523	A→G	A82A (GCT→GCC)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,523	0	A	G	98.8%	264.1 / NA	82	A82A (GCT→GCC)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (13/68);  total (14/68)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATG  >  NC_000913/3815282‑3815769                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            cgccgcGCCCCCGTTCCTGACGAGCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGt                                                                                                                                                                                                                                                       <  2:566388/247‑1 (MQ=255)    cgcgCCCCCGTTCCTGACGATCAAGCGAAATCAATCCGCCAGCAAGCTGCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtc                                                                                                                                                                                                                                                <  2:515153/251‑2 (MQ=255)    cgcgCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtc                                                                                                                                                                                                                                                <  1:52170/251‑2 (MQ=255)     gcgcCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtcg                                                                                                                                                                                                                                               <  2:696029/251‑3 (MQ=255)      cgctcccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAgtggtcgc                                                                                                                                                                                                                                              <  1:729566/245‑4 (MQ=255)        cccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGGTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCaa                                                                                                                                                                                                                                            <  1:351316/247‑1 (MQ=255)        cccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCCTGATGCCCCGCCAGCGCCAcgg‑cgggagtgggcccc                                                                                                                                                                                                                                             >  1:515487/5‑239 (MQ=255)         ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAAt                                                                                                                                                                                                                                           <  2:240874/248‑1 (MQ=255)               cccTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACAGCATCTACCAGCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTTCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑At                                                                                                                                                                                                                                    <  2:175822/250‑1 (MQ=255)                ccTGACGATCAAGCGAAATCAATCCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCTCGG‑CGGTAGTGGTCGCAATCGGG‑ATc                                                                                                                                                                                                                                   <  1:671277/251‑1 (MQ=255)                  ggACGATCAAGCGAAATCAATACGCCAGCAANCGTCGCCCCATTCGCCTGAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGCTCACATCATTTTCCAGCATTACGCGACCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATccc                                                                                                                                                                                                                                 <  2:368143/250‑1 (MQ=255)                     cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTGGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCttg                                                                                                                                                                                                                              <  2:487258/251‑2 (MQ=255)                       atcaaGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGt                                                                                                                                                                                                                            <  1:345796/247‑1 (MQ=255)                             cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAg                                                                                                                                                                                                                      <  1:221125/251‑1 (MQ=255)                             cGAAATCAAAACGCCAGCAAGCGGCGCGCCATTAGCCTGAATAATCTCCAGCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTACCGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGAGGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAg                                                                                                                                                                                                                      <  2:475513/251‑1 (MQ=255)                               aaaTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAggg                                                                                                                                                                                                                    <  1:267607/251‑1 (MQ=255)                                aaacaatacGCCAGCAAGCGTCGCGCCATTANCCTGAATAATCTCCATCGATGCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCGCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGc                                                                                                                                                                                                                   <  2:350629/244‑1 (MQ=255)                                 aTCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGcc                                                                                                                                                                                                                  <  1:145037/251‑1 (MQ=255)                                          ccAGCAAGCGTCGCGCCAGTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTCCCAGCATGACGCGTCCTTGTAACGCGCCACCAACCAGATTGCCGCCTTCACCGTTGTCTTTTGCCTCTTTGCGGGTAAAGCAGCACGGCAGTTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAAcagcag                                                                                                                                                                                                         <  2:244346/251‑1 (MQ=255)                                              cAAGCGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAAGCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCg                                                                                                                                                                                                     <  2:577760/251‑1 (MQ=255)                                                aGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTCATGATTCTCCGCCCGCGCCACGG‑CGGGAGTGGTCGCAAACGGG‑GTCCCCTTGTAAGCAGGGCCaaaaagacgaacga                                                                                                                                                                                                    >  1:84872/1‑239 (MQ=255)                                                      gcgcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCGTTTGCCTCTTTGGGGTTAAAGCAGTACGTCAGGTCAAGGGCATGATGCTCCGCCAGCGCGTCGG‑CGGTAGTTGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAAt                                                                                                                                                                                             <  1:435270/251‑1 (MQ=255)                                                        gcCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCAGTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGc                                                                                                                                                                                           <  1:387123/251‑1 (MQ=255)                                                         ccATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCTTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGcc                                                                                                                                                                                          <  1:708632/251‑1 (MQ=255)                                                              agCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCCTGATGCTCCGCCCGCGCC‑CAGGCGGGTGTGGTCGCAAACGGGGATCC‑TTTGTAAGCCGGGGCAAAcacccggtcggaatcaatgcccaaa                                                                                                                                                                                      >  1:50363/2‑227 (MQ=255)                                                                     ataatCTCCACCGATGCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCCTTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCGGCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGTCCAATCAGCAGATCGAACTCAATGCCAGAATCCTCCaa                                                                                                                                                                              <  2:212675/251‑1 (MQ=255)                                                                            ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCt                                                                                                                                                                          <  1:517035/248‑1 (MQ=255)                                                                                cgattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGt                                                                                                                                                                   <  1:516382/247‑1 (MQ=255)                                                                                            cGCCTTTCCGGCGGGGATCACAGCATCTACCAGCATTACGCGTCCTTTTAACGCGCTACCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCGCGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCt                                                                                                                                                        <  2:256392/250‑1 (MQ=255)                                                                                            cGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTa                                                                                                                                                       <  1:172190/251‑1 (MQ=255)                                                                                             gCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTaa                                                                                                                                                      <  1:569429/251‑1 (MQ=255)                                                                                                   ccGGCTGTGATCCCATCATCTACCAGCATTACGCGGCCTTGTAACGCGCGACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCTCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGc                                                                                                                                               <  2:654657/251‑1 (MQ=255)                                                                                                   ccGGCGGTGATCACATCATCTACCAGCATTANGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGc                                                                                                                                               <  2:355911/251‑1 (MQ=255)                                                                                                         gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGGAGTGGTCGCCATCCGG‑ATCCCTTTGTAAGCCGGGCCCAACAGCCGATCGAACCCCATGCCAGAAACCACCAATGCTTCAGCGTAAAAACGGCCCAAC‑AGTGcccaggc                                                                                                                                         >  1:377878/1‑246 (MQ=255)                                                                                                           gATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGcc                                                                                                                                                                                          <  1:151135/201‑1 (MQ=255)                                                                                                              cccatcatCTAACAGCATTACGCGTCCTTGTAACGCGCTACTAACCAGAGTGCCGCCTTCACCGTGGTCTTTCGCCGCTTGGCGGTTAAAGAAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑ATTGCC‑AGGTCGCGcc                                                                                                                                   <  2:331598/249‑1 (MQ=255)                                                                                                              cacaTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCgcgc                                                                                                                                    <  1:461766/250‑1 (MQ=255)                                                                                                               ccatcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGccc                                                                                                                                  <  2:536933/250‑1 (MQ=255)                                                                                                                           aGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGGCGCCATCGGG‑GTCCCTTTGTTAGCAAGGGCAAACCGCAGATCGCACTCCATGCCAGAATCCCCCAATGCTTCCGCGTAAAAACCGCCTAACCAG‑GCC‑AAGTCGCGCCCCGGATTAAAc                                                                                                                        >  1:706601/1‑249 (MQ=255)                                                                                                                            gCATTTCGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGc                                                                                                                     <  2:84872/251‑1 (MQ=255)                                                                                                                                tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCg                                                                                                                  <  1:126447/250‑1 (MQ=255)                                                                                                                                    cgTCCTTGTACCGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCAGGATGCGCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt                                                                                                             <  2:515487/251‑1 (MQ=255)                                                                                                                                    cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCCGCGCCACGG‑CGGGAGTGGTCGCCATCCGG‑AACCCTTTGTAAGCCGGGGCCAACAGCAGATCGAACTCCATGCCAGAATCCACCAAAGCTTCCGCGGAAAAAACGCCTAAA‑AATGCC‑CGGTAGCGCCCGGCATTAAACAGCCCGGCGtt                                                                                                             >  2:66617/1‑251 (MQ=255)                                                                                                                                    cgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGtt                                                                                                             <  2:3697/251‑1 (MQ=255)                                                                                                                                     ggccTTGTAACGCGCTACCAACCAGATTGCCNCCTTCACCGGGGTCTTTTGCCTCTGTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCAACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGATCTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCTGTATTAAACAGCCCGGCGTTg                                                                                                            <  2:342186/249‑1 (MQ=255)                                                                                                                                       cctcgTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                                                                                          <  1:683579/247‑1 (MQ=255)                                                                                                                                                  gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGc                                                                                               <  1:348279/251‑1 (MQ=255)                                                                                                                                                        aaccaGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgc                                                                                         <  2:307266/251‑1 (MQ=255)                                                                                                                                                           caGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGTCGCAATCGGG‑ATCCCTTTTTAAGCCGGGCCAAACCGCAGAACGAACTCCATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCCAAC‑AATGCC‑CGGGCGCGCCCGGTATTAAACAGCCCCGCGGTGAAGAAATAAGGGCTTTTgcggc                                                                                      >  1:654874/1‑249 (MQ=255)                                                                                                                                                            aGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGccc                                                                                     <  2:763100/251‑1 (MQ=255)                                                                                                                                                                 gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑tt                                                                               <  1:409945/251‑1 (MQ=255)                                                                                                                                                                 gccgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCCAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAACCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCAGGA‑tt                                                                               <  1:449889/251‑1 (MQ=255)                                                                                                                                                                   cgccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGGGGGCGCCATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGGATTAAAAAGCCCGGCGTTGGAGAAAAAAGGGCTTTTGCGCCCGGG‑tttt                                                                             >  1:532983/1‑250 (MQ=255)                                                                                                                                                                        tCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGc                                                                          <  1:12760/249‑1 (MQ=255)                                                                                                                                                                          aCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGAAGTGGTCGCCATACGG‑ATCCCTTTGTAAGAAGGGGCAAACAGCAGAACGAACTCAAAGCCAGAAACCACCCATGCTTCAGCGTAAAAACGGGCTAAC‑AATGCCCAG‑GCGCGCCCGGTATTAAACAGACCGACGTGTAAGAAATAGGGGCTTTTTCGCCCGGAGTTTCacgta                                                                        >  1:555646/1‑246 (MQ=255)                                                                                                                                                                           ccGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTaaa                                                                     <  1:254614/251‑1 (MQ=255)                                                                                                                                                                             gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCATAATCCACCAATTCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt                                                                   <  1:287802/251‑1 (MQ=255)                                                                                                                                                                             gTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGTCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACt                                                                   <  1:111336/251‑1 (MQ=255)                                                                                                                                                                               ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                  <  1:380240/250‑1 (MQ=255)                                                                                                                                                                               ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                  <  1:496505/250‑1 (MQ=255)                                                                                                                                                                               ggTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCg                                                                 <  1:326281/251‑1 (MQ=255)                                                                                                                                                                               ggTCTTTTGCCGCTTTGCGGTTAAAGCAGGNCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTc                                                                  <  2:377878/250‑1 (MQ=255)                                                                                                                                                                                  cctgtgactttttGCGGTTAACGCTGTACGNGAGGTTGAGGTCATGATGCTCCGCCAGCGCTACGG‑AGGTAGTCGTCGCAATCGGG‑ATCCCTTGGTAAGCTGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCGCCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTATGCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTAAGCGTAAAATCGcc                                                               <  2:343210/240‑1 (MQ=255)                                                                                                                                                                                   ttttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa                                                              <  2:189182/250‑1 (MQ=255)                                                                                                                                                                                    tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCa                                                              <  1:713375/249‑1 (MQ=255)                                                                                                                                                                                        cctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAc                                                                                                                                                     <  2:70603/159‑1 (MQ=255)                                                                                                                                                                                             ttGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTaa                                                      <  1:430913/248‑1 (MQ=255)                                                                                                                                                                                                 ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                               <  1:188804/251‑1 (MQ=255)                                                                                                                                                                                                  gTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCCCGG‑CGGTAGTGGTCGCTATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGACCTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgct                                              <  1:383456/251‑1 (MQ=255)                                                                                                                                                                                                      aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct                                          <  1:665828/251‑1 (MQ=255)                                                                                                                                                                                                               ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGAACGAACTCAATGCCAGAATCCAACAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                                  >  1:273190/1‑250 (MQ=255)                                                                                                                                                                                                                gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                <  1:535397/251‑1 (MQ=255)                                                                                                                                                                                                                gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                <  1:59480/251‑1 (MQ=255)                                                                                                                                                                                                                  aggtcaaggtcaTGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                              <  1:368407/251‑1 (MQ=255)                                                                                                                                                                                                                     tcaaggtcATGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTAAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAAATCCCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCaa                            >  1:719131/4‑250 (MQ=255)                                                                                                                                                                                                                          gtcatGATGCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCGTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACt                      <  2:239696/247‑1 (MQ=255)                                                                                                                                                                                                                                  gCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg               <  2:286183/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCTCCGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg               <  2:715506/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGCCAGCGCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATg          >  1:602887/1‑251 (MQ=255)                                                                                                                                                                                                                                         cagcgCCACGG‑CGGTAGTGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACCGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCCCTgcatttggt       >  2:400353/5‑243 (MQ=255)                                                                                                                                                                                                                                          agcgCCACGG‑CGGTAGTGGGCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCAACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGCGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAATGGAGCTGATATGGttt      >  2:507852/4‑251 (MQ=255)                                                                                                                                                                                                                                           gcgCCACGG‑CGGGTGTGGGCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCACCAATGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGGGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTc     <  2:654874/249‑1 (MQ=255)                                                                                                                                                                                                                                              ccACGG‑CGGTAGGGGTCGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCCAACAGCAGATCGGACTCAATGCCAGAATCCACCCATGCTTCAGCGTAAAAACGGCCTAAC‑AGGGCC‑AGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAAATTTAACACCTGCTTGCTAAGCGCCAATTCAATAAAATGGCGCTGATATgggtttatg  >  2:145440/1‑244 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                            CGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGG‑ATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAC‑AGTGCC‑AGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGA‑TTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATG  >  NC_000913/3815282‑3815769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑