Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913160,0920CA28.3% 89.7 / 16.8 60L7F (TTG→TTT) gluQglutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/18);  new base A (0/17);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.69e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATTGCCAGAGAACATCGCCGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCG  >  NC_000913/159847‑160338
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                              
gATTGCCAGAGAACATCGCCGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGGG‑TGTCTGTC‑ATCTCTTTGAACaaaa                                                                                                                                                                                                                                                            >  1:672066/1‑250 (MQ=255)
          gaACATCGCCGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAAAATACGGGG‑TGGCTGTC‑AACTTTTTGAACAAAAAATAAGGAggg                                                                                                                                                                                                                                                 >  2:514601/1‑251 (MQ=255)
          gaACATCGCCGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGAAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCggg                                                                                                                                                                                                                                                 <  1:601297/251‑1 (MQ=255)
                ccccGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑tt                                                                                                                                                                                                                                          <  2:570475/249‑1 (MQ=255)
                  ccGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCCGGATAGTTTCTGCGGCTCCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑c                                                                                                                                                                                                                                       <  1:396932/251‑1 (MQ=255)
                         aGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAACTGCCGAGCGCGGCGATCCG‑AGGG‑CCCAAA‑TGAAGCTCGCCGG‑AAGGGGGGGGGGGGGAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGGACAAAAAATAAGGCGGGGGCAATTT‑cccaac                                                                                                                                                                                                                                  >  2:558012/1‑247 (MQ=255)
                         aGTGCAGACCGTAATGTTCCAGCGGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑ccc                                                                                                                                                                                                                                     <  1:558012/246‑1 (MQ=255)
                            gCAGACCGTAATGTTACAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCCG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑CGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGCACACAAAATAAGGCGGGAGCA‑ttttcccccgttgg                                                                                                                                                                                                                              >  2:321241/1‑240 (MQ=255)
                                        gTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGGC‑TTT‑TCCGCCCGTGGGTAAA‑GTGATgg                                                                                                                                                                                                                >  1:86107/1‑251 (MQ=255)
                                        gTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑ttttccccccgtgggtaacggggtgg                                                                                                                                                                                                                  >  1:374249/1‑228 (MQ=255)
                                         ttCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGCTTTCTT‑CTATGCGCACCAGCCAG‑CGCCCTTTCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCCG‑CGAG‑ACAAAA‑TGAAGCTCGCCGG‑TAGGAGAGGGGGCGAAGGGGCCAAAATACTGTG‑TGTTTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGGGCA‑ATT‑cccccccttggggaaccagatggg                                                                                                                                                                                                                >  2:263059/1‑230 (MQ=255)
                                                 ggccggggATAGTTTCTGCGGCCCCGGGAACTCC‑AGGGGGGGGGTCGATATTTT‑CTATGCGTCCCGGCCAG‑CGACCTGGCCGGGCGCGGGCCGGCAAATAGCTGCCGAGCGCGGGGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTTTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑t                                                                                                                                                                                                      <  1:396451/245‑1 (MQ=255)
                                                    gcggattAGTTCCTGCGGCACCGG‑AACTTCCAGGGGGGGGGCCGATTTCTT‑CTAGGCGTCCCAGCCAG‑GGCCCTTGCCGGGCGCGGGCCTGAAAATAGCTGCCGGGCGCGGCGATCAG‑AGGG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCAAATATACTGTG‑TGTCTGTC‑ACCTCTTTGAAAAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAAt                                                                                                                                                                                                   <  1:306443/245‑1 (MQ=255)
                                                       ggATGGTTTCGGCGGCCCCGGGACCTCC‑AGGGGGGGGGTCGATATCTT‑CTAGGCTTCCCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CAAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCAAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAAtt                                                                                                                                                                                                  <  1:363018/249‑1 (MQ=255)
                                                            gttccTGCGGCGCCGGGACCTCC‑ACGGGGGGGGCCGATTTCTT‑CTTGGCGTCCCCGCCGG‑CGACTTTGCGGGGCGCGGGCCGGAAAATAGCTGCCGAGCGGGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AGGGAGGGGGGGCGAAGCGGCAAATATACGGTG‑TGTCTGTC‑TTTTTTTTGAAAAAAAAATAAGGCGGGGGTA‑TTG‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTagccag                                                                                                                                                                                            <  1:213123/246‑1 (MQ=255)
                                                                     ccccgggaagagtC‑ACGGGGTGGGACGATATCTT‑CTAGGCGGCCCAGCCAG‑CCCCCTGGCGGGGCGGGACCCTACAAATAGCGGCAGGGCGCGGAGACCAG‑AGAG‑CAAAAA‑TGAAGCGCGCCGG‑AAGGAGAGGGGGCGAAGCGGCATATATACTGTG‑TGTCTGTC‑ATCTTTTGGAACAAAAAATAGGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGttt                                                                                                                                                                                  <  2:552034/239‑1 (MQ=255)
                                                                            ggaactccacgggggggtacGATATCTT‑CTAGGCGTACCGGCCAG‑CGCCCTGGCGGGGCGGGGGCCTGAAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCGGG‑AAGGAGAGGGGGCGAAGCGGCAAATATACGGTG‑TGTCTGTC‑ATCTCTTTGAAAAAAAAAAAAGGGGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTcgcg                                                                                                                                                                            <  1:156606/232‑1 (MQ=255)
                                                                                  c‑ACGGGGCGGGTCGTTATCTTATTAT‑CTTACCACCCGG‑CGCCCTTGCCGGGCGCGCGCCTTCAAATAGCGGCCGAGGGCGGCGACAAG‑AGAG‑CAAAAA‑TGAAGCGCGCAGG‑AGGGAGGGGGGGGGAGGCGGCCAATATACTGTG‑TGTCTGTC‑ATTTTTTTGAAAAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTca                                                                                                                                                                     <  2:397274/251‑1 (MQ=255)
                                                                                      ggggggggTCGATATCTT‑CTATGCGT‑CCCGCCAGGCCACCGTCCGGGGCGAGAGCTCGAAAATGGCTGCGGAGCGGGGCGATAAG‑AGAG‑CAAAAA‑GGAAGCTCCCCGA‑AAGGAGAGGGGGGGAAGCGTCAAATATACGGTG‑TGTCTGTC‑CTCTCTGTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTcagcc                                                                                                                                                                  <  2:53034/246‑1 (MQ=255)
                                                                                        ggggggccGATATCTC‑CTAGGCGTACCAGCGAG‑CGCCCTTGCCGGGCGCGAGCCTGCAAATAGCTGCGGAGCGCGGCGATCAG‑AG‑G‑CCAAAAATGAAGCTCGCGGG‑AAGGAGGGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTcagccag                                                                                                                                                                <  1:324591/244‑1 (MQ=255)
                                                                                        ggCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCCCCCGTGG‑TAAACGTGATGGAACCGCTG‑TTAATAACCCGCCATCTCTTTTTTGCGGATTTtagcccg                                                                                                                                                                >  1:743569/1‑249 (MQ=255)
                                                                                                    ttttcctaggcggccccgccag‑cGCCCTTGCCGGGCGGGAGCCGGCAAATAGCTGCCGGGCGCGGCGATCAG‑AGAG‑CCAAAA‑GGAAGCTCGCCGG‑AAGGAGGGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTTTTTAAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAg                                                                                                                                                      <  1:52397/233‑1 (MQ=255)
                                                                                                       t‑ctATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCCGCGTTTTGCAGTCGAt                                                                                                                                                 >  1:99843/1‑251 (MQ=255)
                                                                                                           aTGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGACGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCC‑TTT‑CCCGCCCGGGG‑TCAACGTGATGGAACGGCTG‑GAATTAGCCAGCCCTCCGTTTTTTGCCGATTTCAGCCCGCGTTTTGACGTCGATGc                                                                                                                                               >  2:562300/1‑250 (MQ=255)
                                                                                                           aTGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGcc                                                                                                                                              >  1:232215/1‑250 (MQ=255)
                                                                                                               gacccggcaag‑cGCCCTTGCGGGGCGGGAGCNGGAAAATAGCTGCGGAGGGCGGCGA‑CAGGAGAG‑CCAAAA‑GGAAGCCCGCGGG‑AGGGAGGGGGGGCGAGGCGGCAAATAT‑CCGTGGTGCCGGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGa                                                                                                                                          <  2:374249/242‑1 (MQ=255)
                                                                                                                     gccag‑cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCt                                                                                                                                    >  1:159341/1‑251 (MQ=255)
                                                                                                                       cag‑cGCCCTGGCGGGGCGCGAGCCTGCAAATGGCTGCCGAGCGCGGCGATCAG‑AGAG‑CAAAAA‑TGAAGCTCGCCGG‑AAGGAGGGGGGGCGAGGCGGCAAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGt                                                                                                                                  <  1:562300/251‑1 (MQ=255)
                                                                                                                            gcccTTGCCGGGCGCGGGCCTGAAAAGAGCTGCAGGGCGCGGCGATCAG‑AGAG‑CCAAAA‑GGAAGCTCGCGGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCggg                                                                                                                              <  1:761949/249‑1 (MQ=255)
                                                                                                                            gACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TTAACGTGGTGGAACGGCTGGTAAT‑AGCCCGCCCTCTGTTTTTCGCGGATTTTAGCCAGCGTTTTTCCGTCGGTGGACCGATCGGCGGTccgg                                                                                                                              >  2:490367/1‑248 (MQ=255)
                                                                                                                                       gcgcGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCCGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCCCAGATCGGCCGTCGGGCGCGC‑CTcc                                                                                                                    >  1:615592/1‑249 (MQ=255)
                                                                                                                                         gggAGCCTGAAAATAGCTGCCGAGCGCGGCGAACAG‑AGAG‑CAAAAA‑GGAAGCTCGCCGG‑AAGGAGAGGGGGCGAGGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGAc                                                                                                                <  1:346346/249‑1 (MQ=255)
                                                                                                                                            ggCCTGCAAATAGCTGCCGAGCGGGGCGATCAG‑AGAG‑CAAAAA‑TGAAGCTCGCCGG‑AGGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAAAAAGGCGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGc                                                                                                             <  1:514601/250‑1 (MQ=255)
                                                                                                                                                ggCAAAGAGCTGCGGAGCGGGGCGATCAG‑‑GAGGCAAAAA‑GGAGGCGCGCGGG‑AAGGGGAGGGGGCGAAGGGGCAAATATATTGTG‑TGTTTGTC‑ACCTCTTTGAAAAAAAAATAGGGCGGGATCT‑TTC‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAAt                                                                                                         <  2:736141/250‑1 (MQ=255)
                                                                                                                                                      tAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TCAAAGTGATGGGACGGCTG‑TAATTAGCCCGCCATCTGTTTTTCGCGAATTTCAGCCAACGATTTGCAGTCGATGCACAGATCGGCTGGCGGGCGCGC‑GTcccgaacgcggaaaccaat                                                                                                   >  1:538307/1‑234 (MQ=255)
                                                                                                                                                                 cgcgGCGAGCAG‑AGAG‑CAAAAA‑TGAAGCGTGCCGG‑AGGGTGAGGGGGCGAAGCGGCCAATGTACGGTG‑TGTCTGCC‑ACCTCTTTAAAAAAAAAAAAAGGGTGGAGCA‑TTC‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGc                                                                                        <  2:536434/251‑1 (MQ=255)
                                                                                                                                                                  ggggggaacAG‑AGGG‑CCAAAA‑GGAAGC‑CGCCGGGAAGGAGGGGGGGGAAAGCGGCCAATTTACTTGG‑GGTCTGTC‑ATCTCTTTAAAAAAAAAATAAGGGGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCa                                                                                       <  2:99843/243‑1 (MQ=255)
                                                                                                                                                                     gcaatcgg‑agag‑CAAAAA‑GGAAGCCCGCGGA‑AAGGGGGGGGGGCAAAGGGGCAAATATACTGTT‑TGTCGGTC‑ATTTTTTTGAAAAAAAAAAAAGGCGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGAGGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGa                                                                                    <  2:483404/244‑1 (MQ=255)
                                                                                                                                                                          cAG‑AGAG‑CCAAAA‑TGAAGCACGCGGG‑AAGGAGGGGGGGCGAAGCGGCCAATATACTGTG‑TTTCTGTC‑TTCTCTTTGAAAAAAAAATAAGGGGGGGGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCg                                                                                <  1:513797/250‑1 (MQ=255)
                                                                                                                                                                           ag‑agag‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGGGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGGATACCAATTTTAACACCGccgggttccc                                                                               >  1:241610/1‑241 (MQ=255)
                                                                                                                                                                                    caaaaagaaagcgccgggg‑AAGGAGAGGGGTCGAACCAGCAAATAT‑CTGTT‑TTTTTGTCCATCCCTTAGAACAAAAAACAGGGGGGGAGCA‑TTC‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGcc                                                                      <  1:47341/234‑1 (MQ=255)
                                                                                                                                                                                        a‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTTCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCCGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GGAGCCGGAAt                                                                 >  1:380444/1‑251 (MQ=255)
                                                                                                                                                                                        a‑TGAAGCCCGCGGG‑AGGGAGAGGGCGCGAGGCGCCAAATATACTGTT‑GGTCTGTC‑CTCTTTTTGAAAAAAAAATAAGGCGGGTTCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTCCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATGCCAATTTCAACACCGCAGGATTCGC‑C‑GTAGCCGAAAt                                                                 <  1:397920/251‑1 (MQ=255)
                                                                                                                                                                                          tgcaGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAACTAAGGCGGGAGCA‑TTT‑TCCGCCTTTGG‑TAAACGCGATGGAACGGCTG‑TAATTTGCCCGCCCTCTGTTTTTCGCGAATTTCTGCCAGCGGTTTGCAGGCGATGCCCAGAACTGCTGTCTGGGGCGCCTTCC‑GGCGGAGAATACCAATTTCAACACCGCAggctttgccagaaccgaaatt                                                                  >  2:486968/4‑232 (MQ=255)
                                                                                                                                                                                                  gCCGG‑AAGGAGAGGGGGCGAAGCGGCAAATATGCTGTT‑TGTCTTTC‑ATCTCTTTGAAAAAAAAATAAGGCGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCtt                                                       <  1:551020/251‑1 (MQ=255)
                                                                                                                                                                                                            agagGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCCGCGTTTTGCAGTCGATGCACAGATCGGCCGTCGGGGGGCC‑TTCCCGACGGCGAATACCAATTTCAACACCGCCGGGTTTGC‑A‑GGAGCCGAAAACTTCTGT‑TTCCACttttt                                              >  2:496987/1‑251 (MQ=255)
                                                                                                                                                                                                              ggggggCGAATCGACCAATATACTGTG‑TGTCTGTC‑ATTTCTTGAAAAAAAAAAAAAGGCGGGATCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTc                                            <  1:117491/250‑1 (MQ=255)
                                                                                                                                                                                                                ggggCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTTCAGTCGATGCACCGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGACTACCCATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTc                                            >  1:622427/1‑249 (MQ=255)
                                                                                                                                                                                                                     gAAGCGGCAAATATACTGTG‑TGTCTGCC‑ATCTCTTTAAACAAAAAATAAGGGGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCaa                                                                                              <  1:658769/197‑1 (MQ=255)
                                                                                                                                                                                                                           gcaaaTATACTGTG‑TGTCTGTC‑ATCTTTTTGAAAAAAAAAAAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGa                               <  1:496987/248‑1 (MQ=255)
                                                                                                                                                                                                                              aatttattgtg‑tgGCAGTC‑ATCTCTTGAAAAAAAAAATAAGGCGGGCACA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCt                            <  1:173869/244‑1 (MQ=255)
                                                                                                                                                                                                                              aaTATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGGGAATTCCCATTTCCACAACGCAGGATTACC‑C‑GAAGCCGAAATCTTC‑GTATTCCACTTTTTTCAGCGTCTTCTCGATCt                            >  1:54223/1‑251 (MQ=255)
                                                                                                                                                                                                                               atatACTGGG‑TGTCTGTC‑ATCTCTTTGAAAAAAAAAAAGGGGGGGAGCT‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCtt                           <  1:321241/251‑1 (MQ=255)
                                                                                                                                                                                                                                 ataCTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAACCCGCCCTCTGTTTTTTGCGAATTTCAGCCAGCGGTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGGGC‑TTCCAGACAGCGAATACCAATTTCAAAACCCCAGGATTCGC‑AAGT‑GCCGAAATCTTC‑GTCTTCCCCTTTTTTCAGCCTCTTCTCGATCtttt                         >  1:612656/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gtggtctgtccATCTCTTTGAAAAAAAAATA‑GGGGGGAGCA‑TTTGCCCGCCTGTGG‑GAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCTTCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGAt                     <  2:237290/247‑1 (MQ=255)
                                                                                                                                                                                                                                        g‑tgtCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTTGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGAAGGCGAATACCAATTTCAACACCGCAGGGTTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTTAGCGTCTTCTCGATCTTTTTGATccg                  >  1:340486/1‑249 (MQ=255)
                                                                                                                                                                                                                                            tctgtc‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAAAGGGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCACGAATTTCAGCCAGCGGTTTGCAGTCGATGCACAGATAGGCTGTCGGGCGCGC‑TTCCAGACGGCGAACACCAATTTCAAAACCCCCGGATTCGCCA‑G‑AGCCGCAAACCTC‑GTCTTCCCCTTTTTTCCACGTCCTCTCGATCTTTTTGAttatctt               >  1:115584/1‑245 (MQ=255)
                                                                                                                                                                                                                                                       ctTTGAAAAAAAAATAAGGCGGGAGCA‑TTC‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTcg        <  1:263059/248‑1 (MQ=255)
                                                                                                                                                                                                                                                         ggtaaCAAAAAATAGGGCGGGAGCT‑GTG‑CCCGCCTTTGG‑TTAACGTGAGGGAACGGTTT‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTACAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TGCCAGTCGGCGAAGACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GGCTTCCTCTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCGCGCGAt    <  2:627353/247‑1 (MQ=255)
                                                                                                                                                                                                                                                          tGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGGTGCACAGATCGGCTGTCGGGCGCGC‑TTCCCGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGaacg  >  1:239863/1‑248 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                              
GATTGCCAGAGAACATCGCCGTCCCAGTGCAGACCGTAATGTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTC‑ACGAGGCGGGTCGATATCTT‑CTATGCGTACCAGCCAG‑CGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAG‑AGAG‑CCAAAA‑TGAAGCTCGCCGG‑AAGGAGAGGGGGCGAAGCGGCCAATATACTGTG‑TGTCTGTC‑ATCTCTTTGAACAAAAAATAAGGCGGGAGCA‑TTT‑CCCGCCTGTGG‑TAAACGTGATGGAACGGCTG‑TAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGC‑TTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGC‑A‑GTAGCCGAAATCTTC‑GTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCG  >  NC_000913/159847‑160338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑