Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,070	Δ1 bp	intergenic (‑28/+31)	alsB ← / ← alsR	D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,069	0	G	.	100.0%	288.6 / NA	60	intergenic (‑27/+32)	alsB/alsR	D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/35);  total (25/35)

CAATTGAGATTGAAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGC  >  NC_000913/4311827‑4312304                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            tttgccgttatcgacgaccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTcc                                                                                                                                                                                                                                     <  1:553833‑M2/20‑1 (MQ=255)  ttgccgttatcgacgaccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCcgc                                                                                                                                                                                                                                   >  1:401291‑M2/230‑251 (MQ=255)    gccgttaacgacgaccaggttgattttgcaaccggatgcgcaataggggctaaccgtgacgccttttttcatcgttctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggttattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGa                                                                                                                                                                                                                                 <  2:408177‑M2/24‑1 (MQ=255)          agcgacgaccaggttgattttgcaacctgatgcgcaatagggtcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcttacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCg                                                                                                                                                                                                                            <  2:555418‑M2/29‑1 (MQ=255)           tcgacgaccaggttgcttttgcaacctgatgcgcaataggggcaaaccgagacgacttttttcatcggtctcgctcaagttaatcaaatcacgcatacgcgctctcgactacagtattaatcttttatgccacattttatgtggagtccttccctgatattacgggcactatttattcaacactctgccgaaaaacaggctgtcgtcagatttgacgtgaTGTAAGGGGCCA‑GTAACTCCGCGAGGGCgg                                                                                                                                                                                                                           >  2:274037‑M2/221‑250 (MQ=255)                  ccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAAt                                                                                                                                                                                                                        >  1:72499‑M2/214‑246 (MQ=255)                  ccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcaggtttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAAt                                                                                                                                                                                                                        <  2:72499‑M2/33‑1 (MQ=255)                     ggttgattttgcaacctgatgcgcaatagggnccaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccactttttatgtggggtcagtccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGTACA‑GTAACTCCGCGATGGCGGAATTACctttg                                                                                                                                                                                                                <  2:375642‑M2/41‑2 (MQ=255)                         gattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                                                                                                                                             <  1:6144‑M2/44‑1 (MQ=255)                         gattgtgcaaccggatggggaataggggccaaccgtgacggcttttgtcatcggtctcgctccagtgaatcaaatcgcgcatacgcgctctcgactacagtatgcatctgttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagtttttacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                                                                                                                                            <  2:495102‑M2/45‑1 (MQ=255)                                 aacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                                                                                                                                                    <  2:69597‑M2/53‑1 (MQ=255)                                   cctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgc                                                                                                                                                                                                  >  1:275410‑M2/197‑251 (MQ=255)                                      gatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                                                                                                                                                                 <  1:721959‑M2/56‑1 (MQ=255)                                        agtgcaataggggcaacccgtgactacttttttaatcggtctcgttccagttaatcaaattacgccttcgcgctctcggctacagtaagcatctttgatgccacattttatgtggggtcattcccttagatttcgggcactatttattcaaaactctgacaaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTg                                                                                                                                                                                             <  2:559303‑M2/60‑1 (MQ=255)                                             aataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                                                                                                                                          >  1:172180‑M2/187‑249 (MQ=255)                                                        accgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                                                                                                                                                             <  1:492299‑M2/76‑1 (MQ=255)                                                             gacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAAtt                                                                                                                                                                         <  2:172180‑M2/80‑1 (MQ=255)                                                              acgtcttttttcatctgtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatatttcgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTGATTTCAGCGCGCCTGGTGAGAAGAAAGCAACAATTgc                                                                                                                                                                       <  1:558094‑M2/82‑1 (MQ=255)                                                                 acttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                                                                                                                                                    <  1:206897‑M2/85‑1 (MQ=255)                                                                       ttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttt                                                                                                                                                               <  1:81066‑M2/90‑1 (MQ=255)                                                                       ttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttt                                                                                                                                                               <  1:228940‑M2/90‑1 (MQ=255)                                                                              gtcacgctccagttaatcaaatcacgcatacgcgttctcgactacagtatgcatcttttatgccccattttatgtggggtcattccctgatattacgggcgctatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCata                                                                                                                                                       <  1:71168‑M2/98‑1 (MQ=255)                                                                                   gctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCatcttaat                                                                                                                                                  >  2:127371‑M2/149‑251 (MQ=255)                                                                                   gctcaagttaatccaatcacgcatacgcgctntcgaatacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacggtcactatttattcaaaactctgacgaaaaacaggcggtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGAGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGTCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTCcatattaag                                                                                                                                                  <  2:334313‑M2/103‑2 (MQ=255)                                                                                     tccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCatattaatat                                                                                                                                                >  1:63542‑M2/147‑251 (MQ=255)                                                                                            aatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTg                                                                                                                                         <  1:192117‑M2/112‑1 (MQ=255)                                                                                             atcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAgctttt                                                                                                                                        >  2:616287‑M2/139‑249 (MQ=255)                                                                                                    cacgcatactcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGTCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATg                                                                                                                                  <  1:584872‑M2/119‑1 (MQ=255)                                                                                                              cgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc                                                                                                                       <  1:673259‑M2/130‑1 (MQ=255)                                                                                                                ctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCg                                                                                                                      >  1:537480‑M2/120‑250 (MQ=255)                                                                                                                    cgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGAc                                                                                                                    <  1:338004‑M2/133‑1 (MQ=255)                                                                                                                      actacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAgaga                                                                                                               <  2:63542‑M2/138‑1 (MQ=255)                                                                                                                                    cttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTAAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTcc                                                                                                 >  2:649433‑M2/100‑251 (MQ=255)                                                                                                                                      tttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GAAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAACGTcc                                                                                                 >  1:679141‑M2/98‑249 (MQ=255)                                                                                                                                           gccacattttatggggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAgcaagg                                                                                          <  2:401291‑M2/159‑3 (MQ=255)                                                                                                                                               cattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                                                                                                                                                    <  2:164706‑M2/85‑1 (MQ=255)                                                                                                                                               cattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGcc                                                                                                                                                                    >  1:164706‑M2/89‑173 (MQ=255)                                                                                                                                                     atgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTa                                                                                 >  1:111717‑M2/83‑250 (MQ=255)                                                                                                                                                            gtcattccctgatattacgggcgctagttatgcaaaacgctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGATACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCtt                                                                         <  1:674617‑M2/176‑1 (MQ=255)                                                                                                                                                            gtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGACGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGAAATATTCtt                                                                         >  1:94349‑M2/76‑251 (MQ=255)                                                                                                                                                              cattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGc                                                                       <  1:127371‑M2/178‑1 (MQ=255)                                                                                                                                                                      gatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGc                                                                 <  1:463771‑M2/184‑1 (MQ=255)                                                                                                                                                                      gatattacgggaactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgggaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGCATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGATGAGCTGGGCGACAGCGACAAAAAAAGCGACCAGAAAGGTTGGTTGTAATATTCTTGACGAGGCAt                                                               >  2:692325‑M2/66‑251 (MQ=255)                                                                                                                                                                       atattacgggcactatttattcaaaactctgacgaaaaacaggctggcgtcagttgtgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTAGTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCAtt                                                              <  2:719827‑M2/187‑1 (MQ=255)                                                                                                                                                                          ttacgggcactatttattcaaaactctgccgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACg                                                           >  2:38813‑M2/62‑251 (MQ=255)                                                                                                                                                                            acgggcactatttattcaaaactctgacgaaaaccaggctgtcgtcagttttgacgtgaGGTCAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGAc                                                         >  2:202942‑M2/60‑251 (MQ=255)                                                                                                                                                                            acgggcactatttattcaaaacgctgacgaanaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGAc                                                         <  2:362681‑M2/192‑1 (MQ=255)                                                                                                                                                                               ggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCta                                                      <  1:52904‑M2/195‑1 (MQ=255)                                                                                                                                                                                gcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCta                                                      >  1:24883‑M2/56‑250 (MQ=255)                                                                                                                                                                                gcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGCAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGACCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGCCCta                                                      >  2:525189‑M2/56‑250 (MQ=255)                                                                                                                                                                                 cattatttattaaaaactctgacgaaaaacaagcgtgcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATGTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGTATTACGACCtaat                                                    <  2:729112‑M2/197‑1 (MQ=255)                                                                                                                                                                                   ctatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGGAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTATTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGCGGCATTACGACCtaata                                                   >  2:558353‑M2/53‑250 (MQ=255)                                                                                                                                                                                               aactctgacgaaaaacaggctgtcgtaagatttgacgtgaGGAAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGAGAGAAGAACTCAACAATTCCGCCGGTTTTTTACATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAAAGGCGTTTCCgg                                      >  1:233329‑M2/41‑251 (MQ=255)                                                                                                                                                                                                     gacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGc                                   <  1:292012‑M2/214‑1 (MQ=255)                                                                                                                                                                                                       agaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTg                              <  1:291977‑M2/219‑1 (MQ=255)                                                                                                                                                                                                                          agttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGAGCGCCTGGTGAGAAGAAATCAACAATTGAGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACACAGACAAACAAAGCGTCCAGCCAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGtgagcaaataatatgataggc           >  1:442184‑M2/14‑244 (MQ=255)                                                                                                                                                                                                                              ttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGt       >  1:539105‑M2/10‑251 (MQ=255)                                                                                                                                                                                                                                 acgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTc    <  1:4869‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                                 acgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTc    >  2:4869‑M2/7‑251 (MQ=255)                                                                                                                                                                                                                                   gtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGc  >  1:7793‑M2/5‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CAATTGAGATTGAAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGC  >  NC_000913/4311827‑4312304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑