Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,212,973	Δ1 bp	intergenic (+27/‑67)	rrlE → / → rrfE	23S ribosomal RNA/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,212,973	0	T	.	100.0%	68.5 / NA	20	intergenic (+27/‑67)	rrlE/rrfE	23S ribosomal RNA/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (3/17);  total (3/17)

GGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATG  >  NC_000913/4212918‑4213222                                                        |                                                                                                                                                                                                                                                          ggTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaa                                                       <  1:288215/251‑1 (MQ=14)  gTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGTTCTCCCCATGCGAGGGTAGGGAACTGCCAGGCATCAAATTAGaaa                                                      <  2:90779/251‑1 (MQ=9)  gTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaa                                                       >  1:28080/1‑250 (MQ=14)       aaTGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTGTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcc                                                  <  2:28080/250‑1 (MQ=18)       aaTGAACCGGGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAccc                                                 <  2:283707/251‑1 (MQ=18)        aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcccc                                                >  1:628904/1‑251 (MQ=21)             ccGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAAGTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTcc                                           <  2:324349/251‑1 (MQ=37)                  aGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATa                                        >  1:458443/1‑249 (MQ=38)                       tAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCCGAAGCGGTCTTATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTTCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGcc                                   <  2:604383/249‑1 (MQ=255)                       tAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg                                  <  2:72548/250‑1 (MQ=255)                       tAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg                                  <  2:601859/250‑1 (MQ=255)                        aaCCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGAGTCAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATGTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCAGGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                 <  2:439711/250‑1 (MQ=255)                            gtACAACGCCGAAGGTGGTGTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACGGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCTGGGttt                            <  2:242631/250‑1 (MQ=255)                             ttcAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCCGGCGGCAGGAGCGCGGTTGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttt                            <  2:448360/248‑1 (MQ=255)                             tACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttt                            <  1:401207/250‑1 (MQ=255)                             tACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtt                             <  1:412009/249‑1 (MQ=255)                               aaaggCCGAGGGTGGTTGGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCGGAACGCAGAAGCGGTTTGATAAAACAGAATTGGCCTGGCGGCAGTAGCTCGGTGGTCCCAGCGGTCCCCATGCCTATCTCAGAAGTGAAACTCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGTGTAGGTAACGGCCAGGCATCAAATAGGAAAAACCCCGGACAATAAGGCCGGGGttgttt                         <  2:316293/247‑1 (MQ=11)                                 aCGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGc                       <  1:333375/251‑1 (MQ=255)                                              tttGGCGGAT‑GAGAGAAGATTTTCAGCCTGNACCAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattatt           <  2:386205/250‑1 (MQ=255)                                                ttgtcggatGAGAGAAGATATTCAGCCAGATACAGAGTAAGTCAGAACGCAGAAGCAGTCTGATAAAACAGAATTTGCCTGGCGGCAGGAGCGCGGTGGTCCCCCCTGACCCCAGGCCGAAGTCAGAAGGGAAACGCCTTAGCGTCGATGGTATTGTGGGGTCACCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTAttt          <  2:424913/242‑1 (MQ=17)                                                 ggCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGATCTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa       <  2:685022/251‑1 (MQ=255)                                                     ggatGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatg     >  1:223756/4‑250 (MQ=255)                                                        tGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAAATGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCatgatg  >  2:246775/1‑250 (MQ=255)                                                        |                                                                                                                                                                                                                                                          GGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATG  >  NC_000913/4212918‑4213222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑