Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,985,816	T→C	intergenic (‑224/‑31)	yqeF ← / → yqeG	putative acyltransferase/putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,985,816	0	T	C	98.4%	223.8 / NA	63	intergenic (‑224/‑31)	yqeF/yqeG	putative acyltransferase/putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (36/25);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑TTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAA  >  NC_000913/2985571‑2986059                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGttt                                                                                                                                                                                                                                                <  1:2975/251‑1 (MQ=255) cGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGttt                                                                                                                                                                                                                                                >  1:549244/1‑251 (MQ=255)  gACAATCACAACGAATTTCATCACAACCTCGCACCAATTCACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATCTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGCAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGttt                                                                                                                                                                                                                                                >  2:78346/1‑250 (MQ=255)     aaTCCCAACGTCTTTCATCACCACCTCCCACCAATTAACAGTACAATAGAAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAACAGAGATATTTAAAACAAAGGAAATATGCTTGAGCACCAGTCAGAGACATACCTGGCACGTAGGGTTTGCACCCACTAACCCACCAATAGAGGGGCAGATATGGCTTTAATCCCCAATACTTAACCTGGTNTCTGGAAAATGGCCATACATT‑CTGTTAAAtt                                                                                                                                                                                                                                            >  2:258197/1‑251 (MQ=255)      aTCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAAGTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAAttt                                                                                                                                                                                                                                           <  2:549244/251‑1 (MQ=255)             cGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAgatg                                                                                                                                                                                                                                    <  1:541209/251‑1 (MQ=255)               tCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAgatgat                                                                                                                                                                                                                                  >  1:469494/1‑251 (MQ=255)                   tcatcaCAACCACGCACCAATTAACAGTGCNATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACg                                                                                                                                                                                                                               <  2:278081/250‑1 (MQ=255)                                gCACCAATTAACCGTGCAATAGTAATTGATTTTTAATTACAGTTATCTTAATTGTTTAAAAAAAATGATTTTTATCACAAAAGACATTTGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGCCTGCAACCACTAACCCACCAATAGAGGGGCAGATAGGGCGTTAATCTCCCATCCTTAACCTGGTTTATGGAAAATTGCCATCCATT‑CTCTTTAATTTGTAGATGATACGTTCtaaaaaacccc                                                                                                                                                                                                                 >  2:351044‑M1/1‑240 (MQ=255)                                     aaTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAAATGGCACGTAGGGTTTGCAAACACTAACCCAACAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATAGCACTCCATT‑CTGTTTAGTTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                                                                                                             >  1:147196‑M1/1‑235 (MQ=255)                                     aaTTAACAGTGCAATAGTAAATGATAGTTCATTACAGTTAACTTACTTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGAAGATATGGCGTTAATCTCCCATACTTAAACTGGTTTATGGTAAATTGCCATCAATT‑ATGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                                                                                                             >  2:574244‑M1/1‑235 (MQ=255)                                         aaCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttg                                                                                                                                                                                                        >  2:129602‑M1/1‑231 (MQ=255)                                           cAGTGCAATAGTAGATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacctttgcc                                                                                                                                                                                                      >  2:328037‑M1/1‑229 (MQ=255)                                              tGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGCAATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGTTGATACGTTCtaaaaaaaccccgacatttgccggg                                                                                                                                                                                                   >  2:218226‑M1/1‑226 (MQ=255)                                                 aaTAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggtt                                                                                                                                                                                                >  2:348374‑M1/1‑223 (MQ=255)                                                    aGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTACTCTCCCATACTTAACCTGATTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAgatgat                                                                                                                                                                                                                                  >  2:126716/1‑214 (MQ=255)                                                    aGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAgatgat                                                                                                                                                                                                                                  <  1:126716/214‑1 (MQ=255)                                                         aTGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTATTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGATTGCAACCACTAACCCACCAATAGAGGGGAAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcat                                                                                                                                                                                        >  1:460898‑M1/1‑215 (MQ=255)                                                            aTTGTTAATTACAGTTATCTTAATTGTTTCAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataac                                                                                                                                                                                     >  2:337287‑M1/1‑212 (MQ=255)                                                               gTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgt                                                                                                                                                                                   <  2:304532‑M1/250‑42 (MQ=255)                                                                ttAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgta                                                                                                                                                                                  >  2:407945‑M1/1‑208 (MQ=255)                                                                ttAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgta                                                                                                                                                                                  >  1:247284‑M1/1‑208 (MQ=255)                                                                  aaTTACAGTTATCTTAATTGTTTAAAAAAAGGGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatg                                                                                                                                                                               >  1:573249‑M1/1‑206 (MQ=255)                                                                      aCAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGTCACGTAGGGTTTGCAACCACTAACCCACCAAGAGAGGGGAAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaacaaaccccgacatttgccggggttgtgagcataccgtaatgcttt                                                                                                                                                                           >  2:298335‑M1/1‑202 (MQ=255)                                                                        aGTTATCTTAATTGTTTAAAAAAAGGGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACCTAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttatt                                                                                                                                                                         >  2:546541‑M1/1‑200 (MQ=255)                                                                            aTCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttatttta                                                                                                                                                                      <  1:158482‑M1/250‑55 (MQ=255)                                                                                 aaTTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggac                                                                                                                                                                <  1:531940‑M1/251‑61 (MQ=255)                                                                                         aaaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgcc                                                                                                                                                        <  1:302312‑M1/251‑69 (MQ=255)                                                                                         aaaaaaaGTGATTTTGATCACAAAGGAAATNTGCCTGAGCAGCAGTCAGAGACATAACTTGCATGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCACATACTTAACCTGGTTTATGGTAAAGTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgc                                                                                                                                                         <  2:254154‑M1/250‑68 (MQ=255)                                                                                               aGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaac                                                                                                                                                  >  2:245560‑M1/1‑177 (MQ=255)                                                                                                     ttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTGGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTGGTAGATGATACGTTCtaaaaaaaccccgacatgtgccggggttgggagcataacggaatgcttattttaccggacgcatcgccgagaacagaat                                                                                                                                             <  2:151537‑M1/250‑80 (MQ=255)                                                                                                      tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaata                                                                                                                                            >  1:138005‑M1/1‑170 (MQ=255)                                                                                                          tCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtc                                                                                                                                       >  1:468811‑M1/1‑166 (MQ=255)                                                                                                           cacaAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcg                                                                                                                                      >  1:377806‑M1/1‑165 (MQ=255)                                                                                                                aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggat                                                                                                                                 >  1:461611‑M1/1‑160 (MQ=255)                                                                                                                  gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatgg                                                                                                                               >  1:249261‑M1/1‑158 (MQ=255)                                                                                                                     atatGCCTGAGCAGCAGTCAGAGACATAGCTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtat                                                                                                                            <  1:374582‑M1/251‑97 (MQ=255)                                                                                                                     atatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaacccagacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatctccgggaacagcatcacgtcgcggctggtat                                                                                                                            >  2:9319‑M1/1‑155 (MQ=255)                                                                                                                       atGCCTGAGCTGCAGTAAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCAGCAATAGAGGGGTATATAGGGCGTTAATCGCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATG‑CTGTTTAATTTGGAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtata                                                                                                                           <  2:362424‑M1/250‑98 (MQ=255)                                                                                                                       atGCCTGAGCAGCAGTCAGAGACATAACAGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgccattttccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccggaaacagaataccttcgcggttcgtatgg                                                                                                                          >  2:385194‑M1/1‑153 (MQ=255)                                                                                                                         gCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTATCCTGGTTTATGGTAAATTGCCCTCCATC‑CGGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccgggggtgtgagaataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggct                                                                                                                        <  1:559324‑M1/251‑101 (MQ=255)                                                                                                                              agcagcAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCta                                                                                                                                                                                                                          <  2:429262‑M1/148‑3 (MQ=255)                                                                                                                              agcagcAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCta                                                                                                                                                                                                                          >  1:429262‑M1/1‑146 (MQ=255)                                                                                                                                  gcagTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgc                                                                                                                >  1:475774‑M1/1‑142 (MQ=255)                                                                                                                                    agTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGTTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaaca                                                                                                             <  1:564669‑M1/251‑112 (MQ=255)                                                                                                                                     gTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaatccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacag                                                                                                            <  2:105661‑M1/251‑113 (MQ=255)                                                                                                                                            aCATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattacc                                                                                                     <  2:43528‑M1/251‑120 (MQ=255)                                                                                                                                                         gtagggtttgCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaagaacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgatac                                                                                        <  1:372279‑M1/247‑133 (MQ=255)                                                                                                                                                                           aaCCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgacaccaattcccagacctgc                                                                        <  2:487123‑M1/249‑149 (MQ=255)                                                                                                                                                                            aCCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgt                                                                     >  1:239613‑M1/1‑100 (MQ=255)                                                                                                                                                                                     tAGAGGGGGAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGGAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgccatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgtttgtgaaccacattacaatacgttcgatcccaattcccagacctgctgcaggcggta                                                             >  2:48462‑M1/1‑91 (MQ=255)                                                                                                                                                                                         ggggTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagcca                                                        <  1:496966‑M1/251‑165 (MQ=255)                                                                                                                                                                                                aTAGTGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggggaacagcattaccatacggtcggaaacaattcccagaccttctatcggcggtaatgcatgtttc                                                  >  2:430954‑M1/1‑80 (MQ=255)                                                                                                                                                                                                       gTTAATTTCCCATACGTAACCTGTGTTATGGTAAATTGCCCTCCATG‑CTGTTTAATTGGTATATGATACGTTCtaaaaaaaccccgaacttggccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtgtggctgtgggtgaacagcatgaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggt                                          <  2:447945‑M1/251‑179 (MQ=255)                                                                                                                                                                                                         tAATCTCCCATCCTTAACCTGGGTTATGGTAAATTGCCCTCCATTCTTG‑TTAATTTGTAGATGATACGTTCtaaaaaaaccccgacagttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtg                                         <  2:506814‑M1/250‑180 (MQ=255)                                                                                                                                                                                                            tctcCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtnggcggtaagccatgttccagtgcggtgacgg                                     >  2:289717‑M1/1‑68 (MQ=255)                                                                                                                                                                                                               cccATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgcgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggaatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgccgggggtaagcccggttccagtgcggcgacgcaat                                  >  1:248330‑M1/1‑65 (MQ=255)                                                                                                                                                                                                                    gctTAGCCTGTTTTATGGTAAATTGCCCCCCATT‑CTGTTGAATGTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatctccgggaacagaataacggcgcggatggtatggctgttggtgatcagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttc                              <  2:192499‑M1/249‑191 (MQ=255)                                                                                                                                                                                                                             ggTTTATGGTAAATTGCCCGCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgccgtcggcggtaagccatgttccagtgcggtgacgtaatcttcctcgtagaac                    >  2:418762‑M1/1‑51 (MQ=255)                                                                                                                                                                                                                                          ttGCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacatcgcttcgtcgt       >  1:471738‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                            gCCCTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacatcgcttcgtcgtca     <  2:214978‑M1/251‑216 (MQ=255)                                                                                                                                                                                                                                             cccTCCATT‑CTGTTTTATTTGTGGATGATTCGTTCtaaaaaaaccccgccagttgccggtgttgtgagcataacgtaatgcttattttgccggacgcatcgccgggaacagaataacgtctcggatggtatggctggtggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacatcgcttcttcgtca     <  2:581292‑M1/250‑216 (MQ=255)                                                                                                                                                                                                                                               cTCCATT‑CTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacatcgcttcgtcgtcaccc  >  1:507255‑M1/1‑33 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATT‑TTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAA  >  NC_000913/2985571‑2986059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑