Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,417,047	A→C	intergenic (+156/‑34)	pta → / → yfcC	phosphate acetyltransferase/putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,417,047	0	A	C	100.0%	229.8 / NA	64	intergenic (+156/‑34)	pta/yfcC	phosphate acetyltransferase/putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/42);  total (22/42)

AACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCT  >  NC_000913/2416808‑2417283                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             aaCGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTa                                                                                                                                                                                                                                   <  1:3257‑M1/251‑2 (MQ=255)         gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataac                                                                                                                                                                                                                             <  1:525277‑M1/249‑8 (MQ=255)         gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataac                                                                                                                                                                                                                             <  1:143468‑M1/249‑8 (MQ=255)            ccGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctca                                                                                                                                                                                                                        <  1:434045‑M1/251‑13 (MQ=255)             cGTGGCGCACTGGTTGACGATATCGTCTACAACATCGCGCTGACTGCGATTCAGTCTCCACAGCAGCAGTCATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTCTTCCCTTAATTTGCATTAATCCTTTCTGTTTATCTTGCTTAACTGCGCTGCATCAATGACTTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcac                                                                                                                                                                                                                       >  2:233553‑M1/1‑238 (MQ=255)                ggCGCACTGGTTGACGATATCGTCTACACCATCACGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagt                                                                                                                                                                                                                     <  1:119148‑M1/250‑16 (MQ=255)                         gTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggct                                                                                                                                                                                                           <  2:129557‑M1/251‑26 (MQ=255)                         gTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCAGGTTTGTTATTCTCTGAATTTGCATTAATCCTTTCTGATTATCGTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCCCTTTGCATACTGCCCACTTTGTTacataaccctcaagttaaaatggct                                                                                                                                                                                                           <  2:457839‑M1/251‑26 (MQ=255)                              cGATATCGTCTACACCATCGCGCTGACTGCGATTCAGCCTGCACAGCAGCAGTAATCTCGTCAGCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggctttttt                                                                                                                                                                                                      <  2:543517‑M1/251‑31 (MQ=255)                              cGATAACGTCTACTCCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTTCGCTGCGTATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTTCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggccttttt                                                                                                                                                                                                      <  2:395324‑M1/251‑31 (MQ=255)                                      tatacAACATCGCGTTGACTGCGATTCAGTCTGCTCAGCAGCATTAATCTCGTCATGATCCGCAGCTTTGCGCTTCCGATATCTGAACCGGAAATAATCTCTATTTCCGGTTTTGTAGTCTCTTTATGTGCATTAATCCTGTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataatccacaagggaaaatggcttttttatgttgt                                                                                                                                                                                               <  2:528720‑M1/248‑38 (MQ=255)                                         acacCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgt                                                                                                                                                                                             <  1:9274‑M1/249‑40 (MQ=255)                                            ccATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgt                                                                                                                                                                                        <  1:112283‑M1/251‑45 (MQ=255)                                              aTCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgt                                                                                                                                                                                      >  1:501691‑M1/1‑205 (MQ=255)                                                            gATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgacaatggcttttttatgttgtgtattgtgttgtgtgtttgcagg                                                                                                                                                                        >  2:411928‑M1/1‑191 (MQ=255)                                                                cAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctct                                                                                                                                                                    <  2:41470‑M1/251‑65 (MQ=255)                                                                cAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgcttgcaggctc                                                                                                                                                                     <  1:144570‑M1/250‑64 (MQ=255)                                                                    cTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgngtattgtgttgtgtgtttgcaggctctttc                                                                                                                                                                 >  2:283091‑M1/1‑183 (MQ=255)                                                                         cagcagcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcag                                                                                                                                                           <  1:284987‑M1/251‑74 (MQ=255)                                                                         cagcagcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACGTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcag                                                                                                                                                           <  2:50687‑M1/251‑74 (MQ=255)                                                                             agcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtct                                                                                                                                                       >  1:455608‑M1/1‑174 (MQ=255)                                                                                          tcatcaTCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctc                                                                                                                                                                                                                         <  1:385954‑M1/172‑12 (MQ=255)                                                                                          tcatcaTCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctc                                                                                                                                                                                                                         >  2:385954‑M1/1‑161 (MQ=255)                                                                                              catcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgac                                                                                                                                      <  1:564538‑M1/251‑95 (MQ=255)                                                                                                 ccGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttttgccatcttgacgacaac                                                                                                                                   >  2:83232‑M1/1‑154 (MQ=255)                                                                                                     aGCTTTGCGCTGCGGATATCTGAAACGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagt                                                                                                                                >  1:460485‑M1/1‑150 (MQ=255)                                                                                                      gCTTTGCGCCGGGGAGATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagta                                                                                                                               >  1:43819‑M1/1‑149 (MQ=255)                                                                                                         ttGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaac                                                                                                                             <  1:506444‑M1/249‑104 (MQ=255)                                                                                                           gcgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacatt                                                                                                                          <  1:27214‑M1/250‑107 (MQ=255)                                                                                                                 cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgtt                                                                                                                   <  1:318235‑M1/251‑114 (MQ=255)                                                                                                                    ctatcTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCCTACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcattcaggtcttatgccatcttgacgtcaacagtaacattcaacgttaa                                                                                                                 >  2:7783‑M1/2‑135 (MQ=255)                                                                                                                          gAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgt                                                                                                           <  1:371252‑M1/250‑122 (MQ=255)                                                                                                                           aaCCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtta                                                                                                         <  1:94901‑M1/251‑124 (MQ=255)                                                                                                                                     aatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgt                                                                                                <  1:543558‑M1/250‑133 (MQ=255)                                                                                                                                      atgactaTTTCCGGATTTTTATTCTCTTAATTTGCCTTAATCATTTCTGATTATCTTGCTTAACTGCGCTGTATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaactggcttttttatgttgtgttttgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgaagacaacagtaacattcaacgctaaatctgttaataagacgttg                                                                                              >  2:109935‑M1/4‑117 (MQ=255)                                                                                                                                         aCTAGTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATGGCGCCATCTCACGTTGCATTCTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcat                                                                                           <  2:457822‑M1/251‑138 (MQ=255)                                                                                                                                          cTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgttttgtgtgtttgcaggctctttcgtgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatttgttaataagacgttgcatt                                                                                          >  2:431672‑M1/1‑113 (MQ=255)                                                                                                                                            aTTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacatgcaacgttaaatatgttaataagacgttgcatta                                                                                         <  1:141235‑M1/250‑140 (MQ=255)                                                                                                                                             tttCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattatt                                                                                       >  1:265355‑M1/1‑110 (MQ=255)                                                                                                                                               tCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcgtgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattg                                                                                      <  1:213808‑M1/250‑143 (MQ=255)                                                                                                                                                   gTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGTATCAATTAATTGCGCCATCTCACTTTGCCTACTTCCCACTTTGTTacataaccctcaagtgaaaagggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcataattgtcctg                                                                                 <  2:455777‑M1/251‑148 (MQ=255)                                                                                                                                                        ttATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTTCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACGTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattttgttgtgtgtttgcaggctctttcatgcaggtcttattcgatcttgacgacaacagtcacattcaacgttaaatatgtttataagacgttgcattattgtcctgaagt                                                                             <  2:123046‑M1/250‑152 (MQ=255)                                                                                                                                                          aTTCTCTTAATTTTCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtggttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtgaataagacgttgcattattgtcgttatgttga                                                                          <  2:326983‑M1/251‑155 (MQ=255)                                                                                                                                                              tctTAATTTACATTAATCCTTTCTGATTATCTTGCGTAACTGCGCTGCATCAATGACTTGCGCCATCTCACTTTGCATACTTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtttattgtgttgggtgtttgccggctctttcatgcaggtcttatgccattttgacgtcaaaagcaacattcaacgttaaatatgttaataagacgttgaattcttgtcctgaagtggc                                                                          >  2:333931‑M1/1‑93 (MQ=255)                                                                                                                                                                 tAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagc                                                                   >  2:510037‑M1/1‑90 (MQ=255)                                                                                                                                                                  aaTTTGCAGTAATCCTCTCTTATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagca                                                                  <  2:2028‑M1/251‑163 (MQ=255)                                                                                                                                                                       gCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtat                                                             <  1:375789‑M1/251‑168 (MQ=255)                                                                                                                                                                       gCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgaattattgtcctgaagttgaagatagcaggtat                                                             >  1:319024‑M1/1‑84 (MQ=255)                                                                                                                                                                               ccTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACGTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttg                                                     <  1:294499‑M1/251‑176 (MQ=255)                                                                                                                                                                                 tttCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttga                                                    >  2:137290‑M1/1‑74 (MQ=255)                                                                                                                                                                                    cTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcg                                                >  1:254171‑M1/1‑71 (MQ=255)                                                                                                                                                                                      gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacagcagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatc                                                 <  2:447905‑M1/248‑180 (MQ=255)                                                                                                                                                                                      gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagtcgttgcattattgtcctgtagtttaatatagcaggtatagcggttgaatc                                                 <  1:448139‑M1/248‑180 (MQ=255)                                                                                                                                                                                         tATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagc                                            <  1:411928‑M1/250‑185 (MQ=255)                                                                                                                                                                                          aTCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCTCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcg                                           <  1:367908‑M1/250‑186 (MQ=255)                                                                                                                                                                                               gCTTAACTGAGCTGCATCAATGAATTGCGCCATCTAACTTTGCATACTTCCCACTTTGTTacataaccctcacgtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaaaagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatggcagcgtttcg                                      >  2:353817‑M1/1‑60 (MQ=255)                                                                                                                                                                                                 ttAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcnggtatagcggttgaatcgcagcgtttcgatt                                   >  2:252478‑M1/1‑58 (MQ=255)                                                                                                                                                                                                          cTGCATCCATGAATTGCACCATCTCACTTTTCATACTTCCCACTTTGTTacataaccctcaagttaaaatggctgttttatgttgtgtattgtgttttgtgtttgcaggctctttcatgcaggtcttatgccttcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcatttttgtcctgaagttgaagagagcaggtatagcggttgaatcgcagcgtttcggttgaaggg                             <  2:41114‑M1/248‑200 (MQ=255)                                                                                                                                                                                                            gCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtgaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcaga                         <  2:481960‑M1/250‑204 (MQ=255)                                                                                                                                                                                                             cATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaa                        >  1:36220‑M1/1‑46 (MQ=255)                                                                                                                                                                                                                       ttGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcg             >  1:237342‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                          cgcCATCTCACTTTGCATACTTCCCACTTTGNTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgagg          <  2:265355‑M1/251‑219 (MQ=255)                                                                                                                                                                                                                                  cACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggatttgatc  <  1:452772‑M1/251‑227 (MQ=255)                                                                                                                                                                                                                                  cACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgaggattt      <  1:537813‑M1/247‑223 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             AACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCT  >  NC_000913/2416808‑2417283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑