Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,417,032	C→T	intergenic (+141/‑49)	pta → / → yfcC	phosphate acetyltransferase/putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,417,032	0	C	T	100.0%	231.2 / NA	64	intergenic (+141/‑49)	pta/yfcC	phosphate acetyltransferase/putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/40);  total (24/40)

TGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTT  >  NC_000913/2416785‑2417275                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    tgcAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCAc                                                                                                                                                                                                                                                  >  1:195368/1‑251 (MQ=255)               aaGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCCTACttccc                                                                                                                                                                                                                                    >  2:543558/1‑248 (MQ=255)                        aaCGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTa                                                                                                                                                                                                                           <  1:3257‑M1/251‑2 (MQ=255)                                gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataac                                                                                                                                                                                                                     <  1:143468‑M1/249‑8 (MQ=255)                                gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataac                                                                                                                                                                                                                     <  1:525277‑M1/249‑8 (MQ=255)                                   ccGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctca                                                                                                                                                                                                                <  1:434045‑M1/251‑13 (MQ=255)                                    cGTGGCGCACTGGTTGACGATATCGTCTACAACATCGCGCTGACTGCGATTCAGTCTCCACAGCAGCAGTCATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTCTTCCCTTAATTTGCATTAATCCTTTCTGTTTATCTTGCTTAACTGCGCTGCATCAATGACTTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcac                                                                                                                                                                                                               >  2:233553‑M1/1‑238 (MQ=255)                                       ggCGCACTGGTTGACGATATCGTCTACACCATCACGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagt                                                                                                                                                                                                             <  1:119148‑M1/250‑16 (MQ=255)                                                gTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggct                                                                                                                                                                                                   <  2:129557‑M1/251‑26 (MQ=255)                                                gTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCAGGTTTGTTATTCTCTGAATTTGCATTAATCCTTTCTGATTATCGTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCCCTTTGCATACTGCCCACTTTGTTacataaccctcaagttaaaatggct                                                                                                                                                                                                   <  2:457839‑M1/251‑26 (MQ=255)                                                     cGATATCGTCTACACCATCGCGCTGACTGCGATTCAGCCTGCACAGCAGCAGTAATCTCGTCAGCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggctttttt                                                                                                                                                                                              <  2:543517‑M1/251‑31 (MQ=255)                                                     cGATAACGTCTACTCCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTTCGCTGCGTATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTTCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggccttttt                                                                                                                                                                                              <  2:395324‑M1/251‑31 (MQ=255)                                                             tatacAACATCGCGTTGACTGCGATTCAGTCTGCTCAGCAGCATTAATCTCGTCATGATCCGCAGCTTTGCGCTTCCGATATCTGAACCGGAAATAATCTCTATTTCCGGTTTTGTAGTCTCTTTATGTGCATTAATCCTGTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataatccacaagggaaaatggcttttttatgttgt                                                                                                                                                                                       <  2:528720‑M1/248‑38 (MQ=255)                                                                acacCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgt                                                                                                                                                                                     <  1:9274‑M1/249‑40 (MQ=255)                                                                   ccATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgt                                                                                                                                                                                <  1:112283‑M1/251‑45 (MQ=255)                                                                     aTCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgt                                                                                                                                                                              >  1:501691‑M1/1‑205 (MQ=255)                                                                                   gATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgacaatggcttttttatgttgtgtattgtgttgtgtgtttgcagg                                                                                                                                                                >  2:411928‑M1/1‑191 (MQ=255)                                                                                       cAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctct                                                                                                                                                            <  2:41470‑M1/251‑65 (MQ=255)                                                                                       cAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgcttgcaggctc                                                                                                                                                             <  1:144570‑M1/250‑64 (MQ=255)                                                                                           cTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgngtattgtgttgtgtgtttgcaggctctttc                                                                                                                                                         >  2:283091‑M1/1‑183 (MQ=255)                                                                                                cagcagcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcag                                                                                                                                                   <  1:284987‑M1/251‑74 (MQ=255)                                                                                                cagcagcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACGTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcag                                                                                                                                                   <  2:50687‑M1/251‑74 (MQ=255)                                                                                                    agcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtct                                                                                                                                               >  1:455608‑M1/1‑174 (MQ=255)                                                                                                                 tcatcaTCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctc                                                                                                                                                                                                                 <  1:385954‑M1/172‑12 (MQ=255)                                                                                                                 tcatcaTCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctc                                                                                                                                                                                                                 >  2:385954‑M1/1‑161 (MQ=255)                                                                                                                     catcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgac                                                                                                                              <  1:564538‑M1/251‑95 (MQ=255)                                                                                                                        ccGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttttgccatcttgacgacaac                                                                                                                           >  2:83232‑M1/1‑154 (MQ=255)                                                                                                                            aGCTTTGCGCTGCGGATATCTGAAACGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagt                                                                                                                        >  1:460485‑M1/1‑150 (MQ=255)                                                                                                                             gCTTTGCGCCGGGGAGATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagta                                                                                                                       >  1:43819‑M1/1‑149 (MQ=255)                                                                                                                                ttGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaac                                                                                                                     <  1:506444‑M1/249‑104 (MQ=255)                                                                                                                                  gcgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacatt                                                                                                                  <  1:27214‑M1/250‑107 (MQ=255)                                                                                                                                        cGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgtt                                                                                                           <  1:318235‑M1/251‑114 (MQ=255)                                                                                                                                           ctatcTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCCTACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcattcaggtcttatgccatcttgacgtcaacagtaacattcaacgttaa                                                                                                         >  2:7783‑M1/2‑135 (MQ=255)                                                                                                                                                 gAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgt                                                                                                   <  1:371252‑M1/250‑122 (MQ=255)                                                                                                                                                  aaCCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtta                                                                                                 <  1:94901‑M1/251‑124 (MQ=255)                                                                                                                                                            aatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgt                                                                                        <  1:543558‑M1/250‑133 (MQ=255)                                                                                                                                                             atgactaTTTCCGGATTTTTATTCTCTTAATTTGCCTTAATCATTTCTGATTATCTTGCTTAACTGCGCTGTATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaactggcttttttatgttgtgttttgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgaagacaacagtaacattcaacgctaaatctgttaataagacgttg                                                                                      >  2:109935‑M1/4‑117 (MQ=255)                                                                                                                                                                aCTAGTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATGGCGCCATCTCACGTTGCATTCTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcat                                                                                   <  2:457822‑M1/251‑138 (MQ=255)                                                                                                                                                                 cTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgttttgtgtgtttgcaggctctttcgtgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatttgttaataagacgttgcatt                                                                                  >  2:431672‑M1/1‑113 (MQ=255)                                                                                                                                                                   aTTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacatgcaacgttaaatatgttaataagacgttgcatta                                                                                 <  1:141235‑M1/250‑140 (MQ=255)                                                                                                                                                                    tttCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattatt                                                                               >  1:265355‑M1/1‑110 (MQ=255)                                                                                                                                                                      tCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcgtgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattg                                                                              <  1:213808‑M1/250‑143 (MQ=255)                                                                                                                                                                          gTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGTATCAATTAATTGCGCCATCTCACTTTGCCTACTTCCCACTTTGTTacataaccctcaagtgaaaagggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcataattgtcctg                                                                         <  2:455777‑M1/251‑148 (MQ=255)                                                                                                                                                                               ttATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTTCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACGTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattttgttgtgtgtttgcaggctctttcatgcaggtcttattcgatcttgacgacaacagtcacattcaacgttaaatatgtttataagacgttgcattattgtcctgaagt                                                                     <  2:123046‑M1/250‑152 (MQ=255)                                                                                                                                                                                 aTTCTCTTAATTTTCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtggttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtgaataagacgttgcattattgtcgttatgttga                                                                  <  2:326983‑M1/251‑155 (MQ=255)                                                                                                                                                                                     tctTAATTTACATTAATCCTTTCTGATTATCTTGCGTAACTGCGCTGCATCAATGACTTGCGCCATCTCACTTTGCATACTTCCCCCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtttattgtgttgggtgtttgccggctctttcatgcaggtcttatgccattttgacgtcaaaagcaacattcaacgttaaatatgttaataagacgttgaattcttgtcctgaagtggc                                                                  >  2:333931‑M1/1‑93 (MQ=255)                                                                                                                                                                                        tAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagc                                                           >  2:510037‑M1/1‑90 (MQ=255)                                                                                                                                                                                         aaTTTGCAGTAATCCTCTCTTATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagca                                                          <  2:2028‑M1/251‑163 (MQ=255)                                                                                                                                                                                              gCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtat                                                     <  1:375789‑M1/251‑168 (MQ=255)                                                                                                                                                                                              gCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgaattattgtcctgaagttgaagatagcaggtat                                                     >  1:319024‑M1/1‑84 (MQ=255)                                                                                                                                                                                                      ccTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACGTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttg                                             <  1:294499‑M1/251‑176 (MQ=255)                                                                                                                                                                                                        tttCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttga                                            >  2:137290‑M1/1‑74 (MQ=255)                                                                                                                                                                                                           cTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcg                                        >  1:254171‑M1/1‑71 (MQ=255)                                                                                                                                                                                                             gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacagcagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatc                                         <  2:447905‑M1/248‑180 (MQ=255)                                                                                                                                                                                                             gATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagtcgttgcattattgtcctgtagtttaatatagcaggtatagcggttgaatc                                         <  1:448139‑M1/248‑180 (MQ=255)                                                                                                                                                                                                                tATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagc                                    <  1:411928‑M1/250‑185 (MQ=255)                                                                                                                                                                                                                 aTCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCTCTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcg                                   <  1:367908‑M1/250‑186 (MQ=255)                                                                                                                                                                                                                      gCTTAACTGAGCTGCATCAATGAATTGCGCCATCTAACTTTGCATACTTCCCACTTTGTTacataaccctcacgtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaaaagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatggcagcgtttcg                              >  2:353817‑M1/1‑60 (MQ=255)                                                                                                                                                                                                                        ttAACTGCGCTGCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcnggtatagcggttgaatcgcagcgtttcgatt                           >  2:252478‑M1/1‑58 (MQ=255)                                                                                                                                                                                                                                 cTGCATCCATGAATTGCACCATCTCACTTTTCATACTTCCCACTTTGTTacataaccctcaagttaaaatggctgttttatgttgtgtattgtgttttgtgtttgcaggctctttcatgcaggtcttatgccttcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcatttttgtcctgaagttgaagagagcaggtatagcggttgaatcgcagcgtttcggttgaaggg                     <  2:41114‑M1/248‑200 (MQ=255)                                                                                                                                                                                                                                   gCATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgtgaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcaga                 <  2:481960‑M1/250‑204 (MQ=255)                                                                                                                                                                                                                                    cATCAATGAATTGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaa                >  1:36220‑M1/1‑46 (MQ=255)                                                                                                                                                                                                                                              ttGCGCCATCTCACTTTGCATACTTCCCACTTTGTTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcg     >  1:237342‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                                                 cgcCATCTCACTTTGCATACTTCCCACTTTGNTacataaccctcaagtgaaaatggcttttttatgttgtgtattgtgttgtgtgtttgcaggctctttcatgcaggtcttatgccatcttgacgacaacagtaacattcaacgttaaatatgttaataagacgttgcattattgtcctgaagttgaagatagcaggtatagcggttgaatcgcagcgtttcgattgaatggcagaaacaaaatgtcgagg  <  2:265355‑M1/251‑219 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    TGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATCACTGAATCCAAACCAACAAGAAGATGGGCAATGCCCGATACGTTGGTGATTATCTTTTTTGTTGCTATTTTAACCAGCCTTGCCACCTGGGTAGTTCCGGTGGGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTT  >  NC_000913/2416785‑2417275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑