Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	196.1 / NA	52	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

AGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCG  >  NC_000913/2112930‑2113421                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tgcttggacattacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACtgcgtgc                                                                                                                                                                                                                                                    >  1:413954‑M2/138‑251 (MQ=255)      ggatattacatactcctggcaggtgtgatcaccgcgctgaaggctctctgccgtgaatgttcgcaaataagtatacaaattacttattaaataataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATAAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCCCTGCGTGCATGcc                                                                                                                                                                                                                                               <  2:441637‑M2/119‑1 (MQ=255)             acattctcctgtcaggtgtgatcaccgcgctgaaggctcgtggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTTCTTCAGAGCATGCTGGGAAATAAGCGGCTCTCAGTTTGTTTAATAGAGGTATTAATGCACG‑CTACCGCACCTGGCTTTACAGCTTCCAGAGCACTGCGTGCATGCCTatgat                                                                                                                                                                                                                                         <  1:301593‑M2/125‑1 (MQ=255)                         caggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGtt                                                                                                                                                                                                                             <  1:293136‑M2/137‑1 (MQ=255)                                         gctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGAACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCgctacac                                                                                                                                                                                                            >  2:522368‑M2/98‑248 (MQ=255)                                            gaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCg                                                                                                                                                                                                         >  2:452446‑M2/95‑251 (MQ=255)                                              atgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaa                                                                                                                                                                                                       <  1:452446‑M2/159‑1 (MQ=255)                                               tgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaa                                                                                                                                                                                                       >  1:313243‑M2/92‑250 (MQ=255)                                                gctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaattataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaaa                                                                                                                                                                                                      <  2:150336‑M2/160‑1 (MQ=255)                                                          gggaatggtcgcaaataagtataaaaagtacgtattaaagtataaaaaacttatccatgcttatctgtttacggctttatTTATTCCATATTACTGCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTGACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCGCTAGCGCTAAACTTGAAAAATTCAAAC‑g                                                                                                                                                                                           <  2:61626‑M2/170‑1 (MQ=255)                                                           ggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCt                                                                                                                                                                                         >  1:364613‑M2/80‑251 (MQ=255)                                                            gaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTa                                                                                                                                                                                        <  1:121516‑M2/173‑1 (MQ=255)                                                            gaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGAATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGCATAATTCAAAC‑GCt                                                                                                                                                                                         >  2:58288‑M2/79‑250 (MQ=255)                                                             aatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTa                                                                                                                                                                                        >  1:213846‑M2/78‑250 (MQ=255)                                                                  tcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTc                                                                                                                                                                                  >  1:538788‑M2/73‑251 (MQ=255)                                                                   cgcaaataagtagacaaagttcttattaaagtataaatagcatatccaggcttatatgcttacggctttatTTATTCCATATTACGTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGACTATGATGTGACGAGCGTTACCCACTCGCGCTAACCCCGAAAAATTCAAAC‑GCTAATTGTc                                                                                                                                                                                  <  2:410355‑M2/179‑1 (MQ=255)                                                                    gcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCtt                                                                                                                                                                                <  2:158363‑M2/181‑1 (MQ=255)                                                                       aataagtatacaaagtccttataaaagtaaanatagcttacccaggcttatatgcttacggatttatTTATTCCATATTACTTCATAGCATGCTTGGAAATAAGCGGCTCTCATTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTAcc                                                                                                                                                                             <  2:261664‑M2/184‑1 (MQ=255)                                                                       aataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTAcc                                                                                                                                                                             >  1:105492‑M2/68‑251 (MQ=255)                                                                          aagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCaa                                                                                                                                                                           <  1:565931‑M2/186‑1 (MQ=255)                                                                          aagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAAt                                                                                                                                                                          <  1:508742‑M2/187‑1 (MQ=255)                                                                                             taaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTAcc                                                                                                                                                                             >  2:198500‑M2/46‑229 (MQ=255)                                                                                             taaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTAcc                                                                                                                                                                             <  1:198500‑M2/184‑1 (MQ=255)                                                                                                   ataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCt                                                                                                                                                 <  1:86628‑M2/212‑1 (MQ=255)                                                                                                     aaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTgg                                                                                                                                               <  1:522368‑M2/214‑1 (MQ=255)                                                                                                     aaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGATCTGGAAACAAGGAAAATCCTgg                                                                                                                                               >  1:110654‑M2/38‑251 (MQ=255)                                                                                                       agagcttagccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGAACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGAGCATGCCTCTGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATGGTCTTACCACTCCGCACTGGAACCAAGGAAAATCATGGa                                                                                                                                              >  2:87304‑M2/36‑250 (MQ=255)                                                                                                             tatccatgcttatatgtttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACtt                                                                                                                                        <  2:78669‑M2/221‑1 (MQ=255)                                                                                                              atccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGCCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTg                                                                                                                                      >  1:536021‑M2/29‑251 (MQ=255)                                                                                                                    gcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaa                                                                                                                                 >  2:71761‑M2/23‑250 (MQ=255)                                                                                                                       tatatgcttacggttttttTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAAGCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATaaaa                                                                                                                               <  2:352325‑M2/230‑1 (MQ=255)                                                                                                                          atgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCt                                                                                                                           >  1:6304‑M2/17‑250 (MQ=255)                                                                                                                            gcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAATCCCTAc                                                                                                                         <  2:179114‑M2/236‑1 (MQ=255)                                                                                                                               tacggttttatTTATTCCATATTACTGCAGNGCATGCTGTTAAATAGGCGGCTCTCAGTTTGATTAATAGAGGTATTAAGGCCCG‑CTACCGCCCCTGTCTTTACAGTTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGc                                                                                                                      <  2:275209‑M2/239‑1 (MQ=255)                                                                                                                                      ttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCg                                                                                                               <  1:71761‑M2/246‑1 (MQ=255)                                                                                                                                                   atTACTTCAGGGCATGCTGTGAAATAAGCGNCTCTCAGTTTGATTAATAGAGGTATTAATGCCCG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGAt                                                                                                  <  2:282090/250‑1 (MQ=255)                                                                                                                                                         tCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGttt                                                                                             <  1:543822/249‑1 (MQ=255)                                                                                                                                                         tCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGttt                                                                                             <  1:336418/249‑1 (MQ=255)                                                                                                                                                         tCAGAGCATGCTGTGAAATAAGCGGCTCGCAGTTTAATTAATAGAGGTATTAATG‑ACGGCTACCGCCCCGGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTCCCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCATACGGCTAACTCGTTGTTATTCTGATGGtt                                                                                              <  2:83184/248‑1 (MQ=255)                                                                                                                                                          cAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTta                                                                                            >  1:217170/1‑249 (MQ=255)                                                                                                                                                          cAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGGATTAATGCCCG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTta                                                                                            <  2:217170/249‑1 (MQ=255)                                                                                                                                                                         aaTAAGCGCCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACGGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAgg                                                                           <  2:473774/251‑1 (MQ=255)                                                                                                                                                                           tAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGa                                                                          >  2:385428/1‑250 (MQ=255)                                                                                                                                                                            aaGCGGCTCTCAATTTGATTAATAGCGGTATTACTGCACG‑CTACCACCCCTGGCTTAACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGCCGAGCGATTCACACTCGCGCTAAACCCGAAACATT‑AAACAGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGACAATCCTGGAAAACTTTGCATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAcacaacaacagga                                                                          >  2:559216/1‑242 (MQ=255)                                                                                                                                                                             aGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGaa                                                                                                                                                               <  1:583329/163‑1 (MQ=255)                                                                                                                                                                             aGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGaa                                                                                                                                                               >  2:583329/1‑163 (MQ=255)                                                                                                                                                                                ggCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTc                                                                    <  1:385428/251‑1 (MQ=255)                                                                                                                                                                                      cAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAACAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAACCTTTGAATAAAACCATACTGCTAAATCGTTGTTATTCTGATGGTTNATATAAAACAACGTCAGGGAGATTCGcaaa                                                               >  2:252354/1‑249 (MQ=255)                                                                                                                                                                                        gTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGcaacaa                                                             >  1:247270/1‑250 (MQ=255)                                                                                                                                                                                                    ggggTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTTCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgctg                                                <  2:413954/249‑1 (MQ=255)                                                                                                                                                                                                    gagGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTTCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGCAAAATTCAAAC‑GCTACTTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGACAACTTTGAATAAAACCCTACTGCTAACTATTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtttttctt                                                >  2:34968/1‑247 (MQ=255)                                                                                                                                                                                                                                   tttACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTTTAATTTTCttt                  >  2:197900/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cACTGCGTGCATGCCTATGATTTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCg  <  2:364613/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      AGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACG‑CTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAAC‑GCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTCTTATCAACCGTTACATCCG  >  NC_000913/2112930‑2113421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑