Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,467	A→G	K31K (AAA→AAG)	zinT →	metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,467	0	A	G	100.0%	211.0 / NA	61	K31K (AAA→AAG)	zinT	metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/37);  total (24/37)

CACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGG  >  NC_000913/2041230‑2041712                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      tgctgggtacgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACagag                                                                                                                                                                                                                                           <  1:215672‑M2/101‑1 (MQ=255)  gctgggtacgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAgg                                                                                                                                                                                                                                          <  2:567381‑M2/102‑1 (MQ=255)  gctgggtacgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGt                                                                                                                                                                                                                                         <  1:113453‑M2/103‑1 (MQ=255)         acgggactgggttgagaaacacggtcgaagattgaatggctggcgctcggcgtaattggcgtcgctgtggtactggtcacgttgggaaaatatcgcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTGACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCGGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACaa                                                                                                                                                                                                                                  <  2:22277‑M2/110‑1 (MQ=255)         acgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtctgcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACaa                                                                                                                                                                                                                                  <  1:169132‑M2/110‑1 (MQ=255)                                gtcgaagattgaatggctagcgttcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaatcccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTtg                                                                                                                                                                                                            <  1:297592‑M2/132‑1 (MQ=255)                                  ggaagagtgagtggctggtgctcgtgttaattgtctgcgcggtggtacttgccacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTTCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCTAGTCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTtgatg                                                                                                                                                                                                         <  2:126291‑M2/135‑1 (MQ=255)                                  cgaagattgaatggctggcgctcggcttaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTtgat                                                                                                                                                                                                          <  2:233346‑M2/134‑1 (MQ=255)                                  cgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTtgat                                                                                                                                                                                                          <  1:261152‑M2/134‑1 (MQ=255)                                           aatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATg                                                                                                                                                                                                <  1:485248‑M2/144‑1 (MQ=255)                                            atggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTCTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATTCCAATGt                                                                                                                                                                                               <  2:486967‑M2/145‑1 (MQ=255)                                              ggcttgcgctctgcgtaattgtcttcgcggtggtactggtcacgttgtgaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCGTTAGGTGTCTTTATGGTTAGCGCTCCTTCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAAAAAAAAGCTTCTAATGGTGTTTTTGATGATGCCAAGGTAc                                                                                                                                                                                             <  2:505275‑M2/147‑1 (MQ=255)                                              ggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTa                                                                                                                                                                                              <  1:167409‑M2/146‑1 (MQ=255)                                                      ctcggcgtaattgtcttcgcggtggcactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCg                                                                                                                                                                                      >  1:458223‑M2/97‑250 (MQ=255)                                                        cggcgtaattgtctgcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAAc                                                                                                                                                                                   <  1:512‑M2/157‑1 (MQ=255)                                                            gtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTc                                                                                                                                                                               >  1:393564‑M2/91‑251 (MQ=255)                                                                         cggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATg                                                                                                                                                                  <  1:449606‑M2/174‑1 (MQ=255)                                                                               tactggtcacgttgggtaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGGGACTGGGATGGAGTCt                                                                                                                                                            >  2:340106‑M2/72‑251 (MQ=255)                                                                                actggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCt                                                                                                                                                            <  1:462347‑M2/180‑1 (MQ=255)                                                                                         gttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGt                                                                                                                                                  >  1:43428‑M2/62‑251 (MQ=255)                                                                                            gggaaaatatctcttcccggcaaaacccgtagttgcgccagttagtcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGttt                                                                                                                                                <  1:484444‑M2/192‑1 (MQ=255)                                                                                              gaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGTATGGAGTCTGGCAATCCGTTTAt                                                                                                                                              >  2:560447‑M2/57‑250 (MQ=255)                                                                                               aaaatagctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATcc                                                                                                                                            <  2:42761‑M2/196‑1 (MQ=255)                                                                                                 aatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTGCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGGCGAACAAAAAGCTGCTAATGGTGGTTTTGATGATGCCAATGTACAAAACCGTACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTAGCCtt                                                                                                                                          <  2:358243‑M2/198‑1 (MQ=255)                                                                                                      ctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTCTCCTTTACTg                                                                                                                                     >  1:18610‑M2/49‑251 (MQ=255)                                                                                                        cttcccggcaaaacccggagtggcgccagttattcaggactcatcaCGATTCGTCTTTTCAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGGTTTTGATTATGCCTATGGACAAAACCGAACGCTCAGAGACTGGTATGGAGTCGGGCAATCCGTTTATCCTTTACTg                                                                                                                                     <  2:512556‑M2/203‑1 (MQ=255)                                                                                                           cccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCaa                                                                                                                                  >  1:148239‑M2/44‑249 (MQ=255)                                                                                                              ggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTgg                                                                                                                             >  2:548502‑M2/41‑251 (MQ=255)                                                                                                               gcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGc                                                                                                                            >  2:137345‑M2/40‑251 (MQ=255)                                                                                                               gcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGc                                                                                                                            <  1:551909‑M2/212‑1 (MQ=255)                                                                                                                 aaaacccgtagttgcgccagttattcatgacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCa                                                                                                                           >  1:231922‑M2/38‑250 (MQ=255)                                                                                                                             tgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCg                                                                                                               <  1:342342‑M2/225‑1 (MQ=255)                                                                                                                                  cagttattcaggactcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTc                                                                                                         <  1:515437‑M2/231‑1 (MQ=255)                                                                                                                                  cagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTc                                                                                                         >  1:119108‑M2/21‑251 (MQ=255)                                                                                                                                       attcaggacgcctcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCagaa                                                                                                     <  2:173331‑M2/235‑1 (MQ=255)                                                                                                                                                  atcaCGCTTCGTGTTTACAAACTGGCTGTTGCTTTAGGTGTATTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCAAACGGCAAGCCCTTAAAAGAGGTCGAACAAAACGCTGCTGATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATACTTTACTGCAAAGTGGAAAACTTGACCCAGTCTTTCAGAAGAAATCGGATGc                                                                                         >  2:166286‑M2/5‑251 (MQ=255)                                                                                                                                                  atcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGCCTTTCAGAAGAAAGCGGATGc                                                                                         >  2:84339‑M2/5‑251 (MQ=255)                                                                                                                                                     aCGATTCGTCTGTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGa                                                                                      <  1:122167‑M2/250‑1 (MQ=255)                                                                                                                                                      cGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAt                                                                                     <  1:358934/251‑1 (MQ=255)                                                                                                                                                               tttACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTAAGCATGGTCATCCCTAACACGGCAAGCCCTTAACAGAGGACGAACAAAAAGCTACTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGATCAGAGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAtaaaactaa                                                                             >  1:380044/1‑250 (MQ=255)                                                                                                                                                                    aaaCTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACAtt                                                                       >  1:94624/1‑251 (MQ=255)                                                                                                                                                                         ggCTGTTGCTTTAGGTGTTTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCt                                                                   <  1:580758/250‑1 (MQ=255)                                                                                                                                                                         ggCTGTTGCTTTAGGTGTCTTTATTGTTATCGCACCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTATTTGATGATGCCAATGTACAAAACCGAAAGCTCAGTGACTGGGATGGAGTCTGGCAATCCGATTATCCTTTACTGCGAAGTGGCCAACTTGACCCCGTCTTTCGGAAGAAAGCGGATGCAGATACAACTAACACATTTGCTg                                                                  >  2:568405/1‑251 (MQ=255)                                                                                                                                                                            tGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGaa                                                                >  1:374738/1‑250 (MQ=255)                                                                                                                                                                               tGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATTCAGATAAAACTAAAACATTTGCTGAAAtt                                                             <  1:459662/250‑1 (MQ=255)                                                                                                                                                                                gCTTTAGGTGTCTTTATTGTTAGCGCGCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTa                                                            <  1:281386/250‑1 (MQ=255)                                                                                                                                                                                   ttAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTaaa                                                          <  1:548502/249‑1 (MQ=255)                                                                                                                                                                                     aGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGatt                                                      >  1:54238/1‑251 (MQ=255)                                                                                                                                                                                           cTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAATCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATAATTATc                                                >  1:299689/1‑251 (MQ=255)                                                                                                                                                                                             ttaaggTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGAGGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATTCATATAAAACGAAATCATTTGCTGAATTTAAAGATTATTATc                                                <  2:72727/244‑1 (MQ=255)                                                                                                                                                                                             ttATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATc                                                <  2:350241/249‑1 (MQ=255)                                                                                                                                                                                                   ttAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGt                                        >  1:165894/1‑251 (MQ=255)                                                                                                                                                                                                           ccTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTaa                                                           <  1:562803/224‑1 (MQ=255)                                                                                                                                                                                                           ccTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTaa                                                           >  2:562803/1‑224 (MQ=255)                                                                                                                                                                                                                  tttCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGatat                          <  1:447574/250‑1 (MQ=255)                                                                                                                                                                                                                              atcaCTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGGTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTAGCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACAGTTGCTGAAATTAAAGATTATTATCATAAAGGTTATGCAACATATATCGAGATGATTGGc             <  2:43428/251‑1 (MQ=255)                                                                                                                                                                                                                                 actcacACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCAtt          >  1:111328/1‑251 (MQ=255)                                                                                                                                                                                                                                     acacGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAgg      >  1:217193/1‑251 (MQ=255)                                                                                                                                                                                                                                     acacGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTCTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAgg      >  1:327078/1‑251 (MQ=255)                                                                                                                                                                                                                                       acGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGAc    <  1:549835/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACgg  <  1:564835/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCATTGAGGACGG  >  NC_000913/2041230‑2041712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑