| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,269,250 | A→G | I9T (ATT→ACT) | ldrA ← | small toxic polypeptide LdrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,269,250 | 0 | A | G | 94.7% | 37.9 / ‑3.4 | 19 | I9T (ATT→ACT) | ldrA | small toxic polypeptide LdrA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (8/10); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01 | |||||||||||
GCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTT > NC_000913/1269012‑1269331 | gCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt > 2:52655/1‑251 (MQ=255)gCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt > 1:393449/1‑251 (MQ=255) aaTCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCAGGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGGTTCGGACGCATAGTGCCATCACGGCAGCCTTACGCCCGTTTTCACCTTACTTGCGGTTACGCCACCAGCTGACAAACGCTGCGGACATAATTCACACAAGGATCGGGGCTGCTAGGACGAGCCAGAAAGTGAAGGCAAACTGagcgg > 2:300761/1‑250 (MQ=255) gttGCTTGAAAATTAAAGTCTTGGCGTCTTTCGTTAAGATTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGATGGCTGTTACCCCGGCGGCAGGTTGCGGGCGAATATTGCCATCCCGGCAGCCGGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGATGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa < 2:311071/249‑1 (MQ=14) cTTGAAAAATAAAGTATTAGCGTTCTGAGTTAAGACTTTTTTCATGGGAGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTTCGGCAGGTTTCGGGCGCATATTGCCATCACGGCAGCATGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTAACAAACGCTCCCGTAATAATTCCCGCCAGGTTCGGTGCTACCAGGTCGTGCCAGATAGTCATGGCAAACTGCGCGAGCGTAAtatac > 1:321768/1‑250 (MQ=17) aaaaTAAATTATTAGCTTTCTGCGTTAAGTCTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAgccgcc < 1:462517/251‑1 (MQ=255) aaaaTAAAGTATTAGCTTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAgccgcc < 1:141927/251‑1 (MQ=255) aaaaaaggattgccgtgcTGCGTAATGACTTTTGGCAGGGGTGCTGTATTCAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCGGGTTTCGGCCGCATATTGCCATCCCGGCAGCCTGACGCCCGTTTTCACCTTCCTTCCGGTGAGGCCACCAGCGGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCtt < 2:187681/234‑1 (MQ=11) aaTAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGGGCGTCCTATAGCCGCCtt > 2:116383/1‑251 (MQ=255) aTAAAGGATGAGCGTTCTGTGTTAAGACTTTTTTCATGGGGGCGGGATACAAAAAAGTCCGCAGGCGGTTTCCCCGGCGGCGGGTTTGGGGCGCAGATGTCCACCACGGAAGCCAGACGCCGGTTGTCTCCTTACTTCCGGTGCCGCCACCGGTTGATAATCGCTGCGGTAATAATTCCCGCCAGTATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg < 1:30599/251‑1 (MQ=9) aaaggatgagcgtgcggggTTAAGTCTTTTTTCAGGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCTAATATTGCCATCCCGGCAGCCTGACGCCCGTTTTCCCCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATGCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCTTCATATAGCCGCCttgt < 2:430477/232‑1 (MQ=14) cTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACAtt > 1:572521/1‑251 (MQ=255) tggttaaggtttttttCTGGGGTGCCGGATACAAAAAAGGCCGCAGGCTTTTCCCTCTGGGGCCGGTTTCGGGGGCATATTGCCATCACGGCAGCTGGACGCCCGTTTTCCCCTTACTCCCGGTTACGCCACCAGCTGAAAAGCGCTGCGGTATAAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCATAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACAt < 1:266110/238‑1 (MQ=11) ggttaaggcgttttccttGGGGGCCGGATACAAAAAAGGCCGCAGGCTGTTTCCCCTGCGGCCGGTTTCGGGCGAATATTGCCATCCCGGCAGCCTGACGCCCGTTTTCCCCTTCCTTCCGGTCACGCCACCAGTTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTAATGGCAAACGGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTg < 2:194822/234‑1 (MQ=11) cGTTAAGACTTTTTTCAGGGGTGCCGGATACAAAAAAGGCCGCAGCCTGTTACCCCTCCGGCCGGTTTCGGGCGCATATTGCCAGCACGGCAGCCTGACGCCCGTTTTCACCTTACTCCCGGTTACGCCACCAGCTGACAATCGCTGCGTTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTg < 1:194274/251‑1 (MQ=14) gTTAAGACTTTTTTCAGGGGTGCCGGATAAAAAAAAGGCCGCAGGCTGTTACCCCGGCGGCGGGTTTCGGGCGCATTGGGCCACCACGGCAGCCTGACGCCCGTTTCCCCCTTACTCCCGGTTACGCCACCAGCTGACAATCGCGGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGc < 2:572521/251‑1 (MQ=14) tatgtcTTTTTGCATGGTCGCCGGATAAAAAAAAGGCCGCAGGCTGTTCCCCCTGGGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCCCTTTCCTTCCGGTTACGCCACCAGCTGCCAATCGCGGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCATGTCGTTTCAGAAAGGCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCg < 1:362584/245‑1 (MQ=11) tCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGa > 1:505172/1‑250 (MQ=38) ggATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATAGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCAAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCAGGCTTGTTGTAATGACAANATTTTGCGGCTATTCTTggagtggctggttt > 2:266260/1‑238 (MQ=11) | GCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTT > NC_000913/1269012‑1269331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |