Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	98.2%	182.8 / NA	55	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/27);  minor base C (0/1);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCG  >  NC_000913/674281‑674764                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        ttcgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGACGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑CCGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCgcagg                                                                                                                                                                                                                                            >  2:328806/1‑251 (MQ=255)      cAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGAc                                                                                                                                                                                                                                       >  1:347902/1‑251 (MQ=255)       aGTACGGTATGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATCTACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTAA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATCCGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGAcc                                                                                                                                                                                                                                      >  2:514075/1‑251 (MQ=255)          agggttgggttgtccggcccccAGTTGACCCTAGAGTTCTTTTTATATACCAGGCCTTTTTTATCCAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTTTCCCGGCGTACAGTTTGCCAGCTCGCGGCTCCTTTCA‑TAACCAAAGCACAGAATTTTGAT‑CTGGGTTTTCATATACGCGATG‑TT‑GTCTTTCGTCC‑ACTGGGCCGG‑AGGGGGGTTGTT‑TTTTCCCGCCACGCCTTCCGCAGGCTGACCaaa                                                                                                                                                                                                                                   <  2:454429/231‑1 (MQ=255)                 ggccggtCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATCCCGGGTCTTTTTAATACGGCGCGTGGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTGGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATGTTGGG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTCCGTCC‑ACGGTGCCGG‑AGCGGTGTTTTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTc                                                                                                                                                                                                                             <  2:552235/244‑1 (MQ=255)                    cGTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGTTAAGAATTGCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑CCGGTGCCGG‑AGGTGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAg                                                                                                                                                                                                                         <  1:343902/251‑1 (MQ=255)                       tggggcaccattctcccGCAGAAGTTTTCTTATACACCAGGCCTTTTTTATACAGTTCGTTGAAGAATTTCGTTTCCAAAC‑GG‑TATTTTTCCGGCGTACAGGTTGCCGGCCCGCGGCTCCAGTCA‑TACCCAAAGCCCAGCATTTTGGG‑TTGGTTTTGCATATACGCTTTT‑TT‑GTCGTACGTCC‑AGGGTGCCGG‑GGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGcc                                                                                                                                                                                                                       <  2:129204/235‑1 (MQ=255)                        cGGGCACCAGTTGCCCGAAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCGCGGTAAAGAATTTCTGTTCCCATG‑GG‑TAGTATTCCGCCGTACAGGTTCCCAGCTCGGGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑GGCGGTGTTTTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCg                                                                                                                                                                                                                      <  1:425772/250‑1 (MQ=255)                         gggCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGa                                                                                                                                                                                                                     >  1:1720/1‑250 (MQ=255)                             cccggTTGACCGAGGAAGGCTTTTTATATCCCAGGCCTTTTTTATCCCGTGCGGTGAAAAATTTCTGGCCCCAAG‑GG‑TAGTATTCCGGCGTACAGGTTGCAGGCTCGCGTCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGGG‑CTGGTTTTTCATAGACGCGATG‑TT‑GTCGTCCGCCC‑ACGGTGCCGG‑AGCGGGGTTGTT‑TTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc                                                                                                                                                                                                                <  2:87040/247‑1 (MQ=255)                                 gtgtgccGCAGAAGTTTTCTTATATCCCGGGCTGTTTTTATACAGCGCGGGAAAGAATTTCT‑TTCCCAAC‑GGGTATTTTCCCGGAGTACAGTTTGCCAGCCCGCTGCGCGAGTCA‑TAACAAACCCCCAGCATTTTGAG‑GTGTTTTTTCATATACGC‑ATGGTG‑GCCGTACGACC‑CCGGTGCGGG‑ATCG‑TGTTTTTGTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCa                                                                                                                                                                                                            <  2:446158/246‑1 (MQ=255)                                    gcccggaGAAGTTTTCTTATATCCCAGGCTTTTTTAATCAAGCGCGGTAAAGAATTTCTGTTCCCAAG‑GG‑TATTATCCCGGCTTACGGGTTGCCAGCTCGGGGCCCCAGTCA‑TAACAAAAGCCCAGCTTTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTCCGTCC‑CGGGGGCCGG‑GGCGGTGTTGTT‑TTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAgg                                                                                                                                                                                                          <  2:95519/244‑1 (MQ=255)                                      ccGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCA‑‑CGCGTCCCAGCCGATCGGCTGCAGGACGtt                                                                                                                                                                                                     >  1:161524/1‑251 (MQ=255)                                              gTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGCATTTCTGTTCCCAAC‑GG‑TAGTATTTCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GACGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGATTTTGcc                                                                                                                                                                                               >  2:65353/1‑251 (MQ=255)                                                cttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                              >  1:409964/1‑250 (MQ=255)                                                      atataCCAGGCCTTTTTTATACAGCTCGGTGAAGCATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATCTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAGCGGCTGCAAGACGTTTTTGCCAAGccaac                                                                                                                                                                                       >  2:71714/1‑247 (MQ=255)                                                        atccagtgctttttttAACCAGCTCGTTTAAGAATTTCT‑TTCCCAACCGG‑AAGTATCCCGGCTTCTAGGTTCCCAGCTCGCGGCCCCA‑TCAATAACCAAACCCCAGCTTTTTGAG‑TTGGTTTTCCATATACGCGATG‑TT‑GTCGTACGTCC‑CCGGTCCCGG‑GGCGTTTTTTTT‑TTTCACCGCCACGCCTTCCGCCGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACg                                                                                                                                                                                      <  2:345295/240‑1 (MQ=255)                                                              ggCCTTTTTTACACAGCTCGGTGAAGAATTCCTGTTCCCACC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGGGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTT‑GCCCAGCATACGCTGGTAGc                                                                                                                                                                              <  1:110852/251‑1 (MQ=255)                                                                 cTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGGGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAAGACGGTTTTGCCCCGCATACGCTGGTAGCgg                                                                                                                                                                            >  2:109115/1‑251 (MQ=255)                                                                          caaGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATCCCGGCGTACAGGTTGCCAGTTCGCGGCTCCAGTCA‑TAACAAAAGCCCAGCATTTGGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑CCGGTGCGGG‑GGCGGTGTTGTT‑TTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCAc                                                                                                                                                                    <  1:418502/248‑1 (MQ=255)                                                                            aGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                                 >  1:292369/1‑251 (MQ=255)                                                                                 ggTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGACGTACAGGTTGCCAGCTCGCGGCTCCAGTCC‑TAACCACAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAACCGCCTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                             >  1:238400/1‑250 (MQ=255)                                                                                   tGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATg                                                                                                                                                          >  2:541674/1‑251 (MQ=255)                                                                                   ggaagattgtCTGTTCCCACC‑GG‑TAGTTTCCCGGCGTACAGGTTGCCAGCCCGCGGCCCCAACCA‑AACCCAAAGCCCAGC‑TTTTGGGTCTGTTTTTTCTTATCCGCGAGT‑TT‑GCCGGAGGTCC‑ACGGGGCCGG‑GGCGTTGTTGTT‑TTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAt                                                                                                                                                           <  2:103549/241‑1 (MQ=255)                                                                                            tCTGTTCCCAAC‑GG‑TAGAATTCCGGCGTACAGGTGGCCAGCTCGCGGCTCCAGTCA‑TACCCAAAGCCCAGCTTTTTGAG‑CTGGTTTTTCATATACGCG‑TG‑TTTGTCGTACTCCC‑ACGGTGCCGG‑GGCGTTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                                                                                                                                  <  1:582261/250‑1 (MQ=255)                                                                                              tGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacg                                                                                                                                               >  1:138486/1‑251 (MQ=255)                                                                                                        cggttattatcccgtcGTACAGTTTCCCAGCTCGGGTCTCCAGTCA‑TACCCAAACCCCAGCATTTTGAC‑CTGGTTTTTCATATCCGCGAGG‑TT‑GTGGTACGTCC‑CCGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGc                                                                                                                                       <  1:338006/236‑1 (MQ=255)                                                                                                                     ggtgtccgggTTGCCAGCTCGCGTCTCCAGTCA‑TAACCAAAGCCCGGCATTTTGAG‑CTGTTTTTTCAAATACGCGTGG‑GT‑GTCGTGCGTCC‑AGGGTCCCGG‑AGCGTTGTTGTT‑TTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                                                                                                                          <  1:491964/243‑1 (MQ=255)                                                                                                                                 gCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGCGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCTGAAGGAt                                                                                                               >  1:35515/1‑250 (MQ=255)                                                                                                                                  ccAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGAt                                                                                                               >  1:542908/1‑249 (MQ=255)                                                                                                                                    aGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGGGTAGTCGAACAGCAGGATAggg                                                                                                           >  1:496327/1‑251 (MQ=255)                                                                                                                                       ccgcgGCTCCAGTAA‑AAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATCCGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTTTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg                                                                                                        <  1:436026/250‑1 (MQ=255)                                                                                                                                        ccgcgcccccagtaaaaacaaaaGCCCGGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCGGG‑GGCGGTTTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAg                                                                                                        <  1:541674/231‑1 (MQ=255)                                                                                                                                                  gTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTATGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCCGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATAACGTCACCGATGGGGCAGTTACGTACGTGGCCCATGAGTAGTCGCCCAGAAGGATAGGGAAGCATAGACAGGc                                                                                             >  1:364025/1‑251 (MQ=255)                                                                                                                                                           caaggCCCAGCATTTTGGG‑CTGTTTTTTCATATACGCGGTG‑TT‑GTCGT‑CGTCCCAGGTTGCCGG‑GGCGGTTTTGTT‑TTGCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCGGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAc                                                                                     <  1:103321/247‑1 (MQ=255)                                                                                                                                                           cAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTCCGTCC‑ACGGTGCCGG‑GGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAc                                                                                     <  1:139452/251‑1 (MQ=255)                                                                                                                                                            aaaGCCCAGCATTTTGAG‑ATGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTCATACt                                                                                    >  2:481853/1‑251 (MQ=255)                                                                                                                                                             aaGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGT‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCCGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGAACGTGGCCCCTGTGTCGTCGACCAGAAGGATAGGGAAGCATAGCCAGGCAGTAATACtt                                                                                   >  2:368293/1‑251 (MQ=255)                                                                                                                                                                   aGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGAGAAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGNGAAGCATAGCCAGGCAGTAATACTTCTCttt                                                                             >  2:267361/1‑251 (MQ=255)                                                                                                                                                                   aGCATGTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTGGTACGCCC‑GCGGTGCCGG‑CGGGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGTGAAGCATAGACAGGCAGTAATACTTCTCttt                                                                             <  1:543833/251‑1 (MQ=255)                                                                                                                                                                          ggAG‑CTGGTTTTTCATATCCGCGATT‑TT‑GTCGTCCGTCC‑ACGGGGCCGG‑GGGGGTGTTGTT‑TTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCtctc                                                                       <  1:328806/249‑1 (MQ=255)                                                                                                                                                                                          tatccggggtg‑tt‑gtCGTCCCTCC‑ACGG‑GCCGGGAGCTGTGGTGTT‑TTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑c                                                       <  2:354660/241‑1 (MQ=255)                                                                                                                                                                                                              acgccc‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAgg                                                                                              <  1:527617/190‑1 (MQ=255)                                                                                                                                                                                                              aCGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATcc                                    >  2:516908/1‑251 (MQ=255)                                                                                                                                                                                                              aCGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGGGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAgg                                                                                              >  2:527617/1‑194 (MQ=255)                                                                                                                                                                                                                 ccc‑ACGGTGCCGG‑GGCGGTGTTGTT‑TTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCaa                                 <  1:258027/250‑1 (MQ=255)                                                                                                                                                                                                                  gtcaccgggcgcgcaGCGGTGTTGTT‑CTACACCGCGCCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATATGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCAGCCCa                                  <  2:111883/237‑1 (MQ=255)                                                                                                                                                                                                                  cc‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTCATT‑CAAATGTGCGCTTTTCTTCCCAAt                                >  2:300560/1‑251 (MQ=255)                                                                                                                                                                                                                  cc‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAAt                                >  1:247712/1‑251 (MQ=255)                                                                                                                                                                                                                     ccGGTGCTGG‑GGCTGTGTTGTT‑TTGCACCGCCACGCCTGCCGCAGGCAGACCAAACGCGCCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCTATCACGTCTCCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGa                              <  1:493582/250‑1 (MQ=255)                                                                                                                                                                                                                       ggTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGa                              >  1:347016/1‑249 (MQ=255)                                                                                                                                                                                                                                  gggttttttt‑ttcCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTg                  <  1:368293/243‑1 (MQ=255)                                                                                                                                                                                                                                   ggTGTTGTT‑TTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTgg                 >  1:161157/1‑251 (MQ=255)                                                                                                                                                                                                                                     gtttgtt‑tgtCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt               <  1:65353/249‑1 (MQ=255)                                                                                                                                                                                                                                      gttgtt‑TTTAACCGCCATGCCTTCCGAAGGCGGACCAAACGAGCCACAGCCGATCGGCTGCAGGACGATCTTGCCCAGCATACGCCGGTAGCGGGCGATCACGTCACCGATGGTGGAGTTACGTACGTGGCCCACGTGTAGTCGCACAGAAGGATAGGGAAGCATAGACAGGCAGTACTACTTCTCTTTGCTCTCGTCTTCGGTTACTTACAAA‑GAGCGCATATCATCCCAATGAAGCTGTAATTTGGAtt              >  2:527837/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cgccacGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCg  <  1:516908/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        TTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAC‑GG‑TAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCA‑TAACCAAAGCCCAGCATTTTGAG‑CTGGTTTTTCATATACGCGATG‑TT‑GTCGTACGTCC‑ACGGTGCCGG‑AGCGGTGTTGTT‑TTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTT‑CAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCG  >  NC_000913/674281‑674764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑