Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,356	A→G	intergenic (‑121/‑16)	fecI ← / → ykiE	RNA polymerase sigma factor FecI/protein YkiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,356	0	A	G	100.0%	195.4 / NA	53	intergenic (‑121/‑16)	fecI/ykiE	RNA polymerase sigma factor FecI/protein YkiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/28);  total (25/28)

GGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCG  >  NC_000913/4518110‑4518602                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAag                                                                                                                                                                                                                                                    <  1:219041/251‑1 (MQ=255) ggCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGATTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAag                                                                                                                                                                                                                                                    <  1:341670/251‑1 (MQ=255)     aTGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAaggtag                                                                                                                                                                                                                                                <  1:214052/251‑1 (MQ=255)       gTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTAAACCAGCCGTGATGTGTGCCATAAAAAGCCTCGAACGTTAAAGAAGCTGAGGTAGTGGCGCGGTCAGACATGCGGCGTGCATCACAAATTAATTATCAAGTAGTCATATTAATATGAGAATGGTTATAATTACAATTGGACATAAAATTGTTTCAAATAGAAATTTTTAACATGTTGTTTTTTTAAGTGTTGTAaggtaggg                                                                                                                                                                                                                                              >  2:391621/1‑250 (MQ=255)         cATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAggtaggta                                                                                                                                                                                                                                             <  1:87765/250‑1 (MQ=255)                    aaaGCATACTGGAGTTGGGGCGGCAGCAATCTTTTCAACCAGACGTGATGGGTGCCATAAGGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGAAGTCATATTAATATGAGAATGGTTATCAGTACAATTGGAAATAAAATTGTTTCCAATAGACATTTGTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGAta                                                                                                                                                                                                                                 <  2:93313/251‑2 (MQ=255)                           aCTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCctc                                                                                                                                                                                                                          <  1:138087/251‑1 (MQ=255)                               gAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTAGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCt                                                                                                                                                                                                                      >  1:58180/1‑251 (MQ=255)                                  tttGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCCAACGTTAATTCTCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCt                                                                                                                                                                                                                   >  1:487760/1‑251 (MQ=255)                                    tGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTg                                                                                                                                                                                                                  <  2:364990/250‑1 (MQ=255)                                     gcgcGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTgg                                                                                                                                                                                                                 <  2:205000/250‑1 (MQ=255)                                       gcgTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGCAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGATATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCAt                                                                                                                                                                                                              >  1:92704/1‑251 (MQ=255)                                            agccagcTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATTGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTc                                                                                                                                                                                                         <  1:570782/251‑1 (MQ=255)                                                   ttttCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCCGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGTCATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGCTGTAGCCTCTGCTTCTGGCATGTGTCGGTc                                                                                                                                                                                                     >  2:575209/1‑248 (MQ=255)                                                                       gtgCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTg                                                                                                                                                                              <  1:580416/251‑1 (MQ=255)                                                                             tAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTAAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActc                                                                                                                                                                        >  1:327454‑M1/1‑248 (MQ=255)                                                                                  cGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGTTGTGGTCTGCTActcaaggt                                                                                                                                                                   >  2:524964‑M1/1‑243 (MQ=255)                                                                                       cGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCGTCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGAAATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCAGGTGTCGGTCAGAAGGACTCATGAAGGGGTCTGCTActaacgggggaat                                                                                                                                                              >  2:347864‑M1/1‑238 (MQ=255)                                                                                           cGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtg                                                                                                                                                          <  2:500302‑M1/251‑18 (MQ=255)                                                                                                          ggTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgcc                                                                                                                                           <  1:270130‑M1/251‑33 (MQ=255)                                                                                                                      gTCAGACATGCGGATTGCATCTAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAAATGTTTCCAATAGACATTGTTAACATGTTGTTTTTCTAAGTGTGGTAAGGTAGGTATAAATTGGGATGGATCCTCTGCTTCGGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTTctcaagttggaattgtgtcgcagtataccgccagaaacggggat                                                                                                                               <  2:327454‑M1/251‑45 (MQ=255)                                                                                                                      gTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgaagtataccgccagaaacggggat                                                                                                                               >  2:143003‑M1/1‑207 (MQ=255)                                                                                                                         aGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggctta                                                                                                                             >  1:394931‑M1/1‑204 (MQ=255)                                                                                                                                  gAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactcttt                                                                                                                    >  1:579754‑M1/1‑195 (MQ=255)                                                                                                                                    gTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacc                                                                                                                 <  1:464760‑M1/251‑59 (MQ=255)                                                                                                                                       cattaaaagataATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGCTGTTTTTCTCAGTGTTGTACGGTAGGTATAAAATGAGATGGAGCCTCTGCTTCTGGCATTTGTCGGTCAGAATGACTCAGGATGTGGTCTGCTActcaaggtggaattgtgtcgccggataacgccagaaacggggtttaatactctttacctgt                                                                                                              >  2:314455‑M1/5‑190 (MQ=255)                                                                                                                                        aTCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgtt                                                                                                             >  2:554422‑M1/1‑189 (MQ=255)                                                                                                                                                ttAATTATCACGTCGTCATATTAATATACGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTTTTGTACGGTAGGGATACAATTGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggaggaattgtgtcgcagtataccgccagccacngggataaatcctctttacctgttacccggct                                                                                                     >  2:275080‑M1/1‑181 (MQ=255)                                                                                                                                                  aaTTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggcta                                                                                                    <  1:60084‑M1/250‑72 (MQ=255)                                                                                                                                                  aaTTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggcta                                                                                                    <  2:46934‑M1/250‑72 (MQ=255)                                                                                                                                                   aTTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacg                                                                                                  <  1:391621‑M1/251‑74 (MQ=255)                                                                                                                                                      aTCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggt                                                                                                >  1:262000‑M1/1‑175 (MQ=255)                                                                                                                                                              gTCATATTAATATGAGAATGGTTATAATTACAATTGGAAATAAAATTGTTTACAATAGAACTTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATAACTCATGATGTGGTCTGCTActcagggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacccgttacccgactacgatagctatccc                                                                                       >  2:415713‑M1/1‑167 (MQ=255)                                                                                                                                                                 atatTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtatacagccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagt                                                                                    >  2:24278‑M1/1‑164 (MQ=255)                                                                                                                                                                     tAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttga                                                                                 <  1:371223‑M1/250‑91 (MQ=255)                                                                                                                                                                           gagaATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcac                                                                          >  1:22397‑M1/1‑154 (MQ=255)                                                                                                                                                                                     aTCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactg                                                                >  1:509196‑M1/1‑144 (MQ=255)                                                                                                                                                                                     aTCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaact                                                                 <  2:58652‑M1/250‑107 (MQ=255)                                                                                                                                                                                       cATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgt                                                               <  1:85198‑M1/250‑109 (MQ=255)                                                                                                                                                                                       cATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgt                                                               <  1:225634‑M1/250‑109 (MQ=255)                                                                                                                                                                                          tACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggacttgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacc                                                           >  1:255525‑M1/1‑139 (MQ=255)                                                                                                                                                                                             aaTTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTGGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtggcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccag                                                        <  2:22397‑M1/251‑116 (MQ=255)                                                                                                                                                                                              aTTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccagg                                                       >  1:468543‑M1/1‑135 (MQ=255)                                                                                                                                                                                                    aaTAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccatgcgcata                                                 <  1:404373‑M1/251‑123 (MQ=255)                                                                                                                                                                                                                  cAATAGACATTGTTAACATGTTGTGTTTCGAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTACTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcg                                   <  2:988‑M1/251‑137 (MQ=255)                                                                                                                                                                                                                    aTAGACATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagatgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgtt                                 >  1:572723‑M1/1‑113 (MQ=255)                                                                                                                                                                                                                         cATTTTTAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccataaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgccc                            >  1:175126‑M1/1‑108 (MQ=255)                                                                                                                                                                                                                             tttAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagc                         <  1:570006‑M1/250‑147 (MQ=255)                                                                                                                                                                                                                             tttAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagc                         <  1:554422‑M1/250‑147 (MQ=255)                                                                                                                                                                                                                              ttAACATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGGCGGTCAGAATGACTCATGCTGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggaagctatcccagatgcagataacccacaaactgttacccaggcgcatacggtccttcaagcgttcgcccagca                        >  2:347551‑M1/1‑103 (MQ=255)                                                                                                                                                                                                                                aaCATGTTGTTTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaac                      <  1:499616‑M1/250‑150 (MQ=255)                                                                                                                                                                                                                                 aCATGTTGTTTTTCGAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcgcccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaactt                    <  2:359804‑M1/251‑152 (MQ=255)                                                                                                                                                                                                                                       tgttTTTCTAAGTGTTGTAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctacggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgcccagcaacttggtcat              >  1:178146‑M1/1‑94 (MQ=255)                                                                                                                                                                                                                                                   tgttgtAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTActcaaggtggaattgtgtcgcagtataccgccagaaacggggattaatactctttacctgttacccggctccggtagctatcccagttgaagatcacccacaaactgttacccaggcgcatacggtccatcacgcgttcgccaagcaacttggtcatctcttccatatt  >  2:443‑M1/1‑82 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GGCAATGTCATCTGCATCAAAAGCAGACTGGAGTTTGCGCGTCAGCCAGCTTTTCAACCAGCCGTGATGTGTGCCATAAAGCGACTCGAACGTTAAGGAAGCTGTGGTAGTGGCGCGGTCAGACATGCGGAGTGCATCAAAAGTTAATTATCACGTAGTCATATTAATATGAGAATGGTTATCATTACAATTGGAAATAAAATTGTTTCCAATAGACATTTTTAACATGTTGTTTTTCTAAGTGTTATAAGGTAGGTATAAAATGGGATGGAGCCTCTGCTTCTGGCATGTGTCGGTCAGAATGACTCATGATGTGGTCTGCTATTATTGACATCCTCACTGCCCTAAAGGATGGGGATTTCGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCG  >  NC_000913/4518110‑4518602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑